| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 2,270,615 | A→G | 29.4% | E279E (GAA→GAG) | yejF → | microcin C transporter, ATP‑binding subunit; ABC family |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 2,270,615 | 0 | A | G | 29.4% | 19.2 / 10.7 | 21 | E279E (GAA→GAG) | yejF | microcin C transporter, ATP‑binding subunit; ABC family |
| Reads supporting (aligned to +/- strand): ref base A (7/7); new base G (3/3); total (11/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.80e-01 | |||||||||||
TGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAA > CP009273/2270467‑2270754 | tGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGaa < 1:341433/149‑1 (MQ=255)tGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGaa < 1:487331/149‑1 (MQ=255) aaaaCGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCATGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGCTGGTGGACAg > 1:50001/1‑149 (MQ=255) tgtgTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAGGTTGc < 1:19010/149‑1 (MQ=255) aaTTACGCCGCTACGCTATTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAGGTTGCCTTCCCCATTCg < 1:243070/149‑1 (MQ=255) ctacgctaTTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAATGCCGTTGCCAGAAACTGCCTCAACGTTGCTGGATGTTAGACAGCTTGCGGTTGCGTTCCCCATTGGCaaa > 2:203022/1‑143 (MQ=255) tttGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTGCAGGTTGCGTTCCCCATTCGCAAAGGGAtttt > 2:61856/1‑143 (MQ=255) ccACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCACCGTTGCTGGATGTTGAGCAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGAt > 1:361802/1‑149 (MQ=255) ccACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAGCAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGAt > 1:408947/1‑149 (MQ=255) ccACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAGCAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGAt > 1:361861/1‑149 (MQ=255) aCTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCa > 1:38688/1‑149 (MQ=255) aCTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGACGTTTCCAGAACCTGCCACCACGTTGCTGTATGTTGACCAGCTTCAGGTTGCCTTCCCCATTCGCTAAGGGATTTTGTTTCGCATTGTGTATct > 1:205537/1‑148 (MQ=255) ttACACACAAAAGCTACTCAACAGTGAACCGTCAGGCAATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATCTTGAAGCGAATTGTGGATCAtc > 2:101895/1‑142 (MQ=255) tACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCAGaa > 2:209419/1‑143 (MQ=255) acacacAAAAGCTACTCAACCGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATgtggtg < 1:463353/149‑1 (MQ=255) tCCAGTGCCGTTGCCCGAAACTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTTAAAAACATCAGTTTTACGCTACGAGCgg < 2:455990/143‑1 (MQ=255) cGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAaca < 2:319030/143‑1 (MQ=255) ttGCTGGATGTTGAACAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCgg < 1:448684/149‑1 (MQ=255) gCTGGATGTTGAACAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGa < 1:467824/149‑1 (MQ=255) gagtttGAGCAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCgg < 2:366752/139‑1 (MQ=255) gATGTTGAGCAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCgg < 2:322370/143‑1 (MQ=255) gATGTTGAGCAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCgg < 2:322425/143‑1 (MQ=255) tGAACAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGaaaa > 2:328129/1‑143 (MQ=255) | TGCAAAACGGTCGCTGTGTCGAGCAAAATTACGCCGCTACGCTATTTGCATCACCCACTCATCCTTACACACAAAAGCTACTCAACAGTGAACCGTCAGGCGATCCAGTGCCGTTGCCAGAACCTGCCTCAACGTTGCTGGATGTTGAACAGCTTCAGGTTGCCTTCCCCATTCGCAAAGGGATTTTGAAGCGCATTGTGGATCATAATGTGGTGGTGAAAAACATCAGTTTTACGCTACGAGCGGGTGAAACACTGGGTTTAGTGGGCGAGTCCGGTTCCGGGAAAA > CP009273/2270467‑2270754 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |