Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	CP009273	3,178,254	IS5 (+) +4 bp	54.3%	coding (56‑59/291 nt)	yqiC →	BMFP family putative fusogenic protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	2282398 =	NA (NA)	12 (0.840)	11/272	NT	59.3%	noncoding (1195/1195 nt)	IS5	repeat region
	?	CP009273	3178254 =	8 (0.580)					coding (56/291 nt)	yqiC	BMFP family putative fusogenic protein
*	?	CP009273	= 2283592	NA (NA)	7 (0.490)	7/274	NT	45.9%	noncoding (1/1195 nt)	IS5	repeat region
	?	CP009273	= 3178257	8 (0.570)					coding (59/291 nt)	yqiC	BMFP family putative fusogenic protein

AGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/2283452‑2283592 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGGCATTGATTCGTGAACCTGGCGAGCGATTTGCTCAATTTTTTTCGGGTCAATCATTGTAGTTTCCCTGTACTGATAGGTGTTGAGTGCCATTGTAGTGCGATAA  <  CP009273/3178257‑3178150                                                                                                                                                                                                                                                           AGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCATT                                                                                                     >  1:241289/1‑150           TTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCATTGATTCTTGAA                                                                                           >  1:236021/1‑150                      GACGGCGCTTACTGCTGAATTCACGGTCGGGGAAGGTAAGCTGTTGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCATTGATTCGTGAACCTGGCGAGCG                                                                                <  1:245863/150‑1                                                 CGGCGAAGGTAAGTGGATGACTCATGATGCACCCTGTTCTATGGCTCCTGGTGACAAACATGATCTCATATCAGGCACTTGTTCGCACCTTCCTTAGGCATTGATTCGTGAACCTGGCGAGCGATTTGCTCAATTTTTTTCGGGTC                                                         <  2:127190/146‑1                                                                         TGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCATTGATTCCTGACCTTTGCGAGCGATTTGCTCAATTTTTTTCGGGTCAATCATTGTAGTTTCCCTGTACTGATAG                             >  1:192376/1‑150                                                                                 CCTGTTCTATGGCTCCAGATGACAAACAGGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCATTGATTCGTGACCCTGGCGAGCGCTTTGCTCAATTTTTTTCGGGTCATTCATTGTCGCTTCCCTTTACTGtcagctgt                         >  2:26846/1‑138                                                                                                    TGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCATTGATTCGGGAACCTGGCGGGCGATTTGCTCAATTTTTTTCGGGTCAATCATTGTAGTTTCCCTGTACTGAGAGGTGTTGAGTGCCATTGTAGTGAGAGAA  >  1:5057/1‑150                                                                                                                                                                                                                                                           AGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  CP009273/2283452‑2283592 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGGCATTGATTCGTGAACCTGGCGAGCGATTTGCTCAATTTTTTTCGGGTCAATCATTGTAGTTTCCCTGTACTGATAGGTGTTGAGTGCCATTGTAGTGCGATAA  <  CP009273/3178257‑3178150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.