| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 764,350 | T→C | 44.4% | L306P (CTG→CCG) | mngB → | alpha‑mannosidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 764,350 | 0 | T | C | 44.4% | ‑3.8 / 7.0 | 9 | L306P (CTG→CCG) | mngB | alpha‑mannosidase |
| Reads supporting (aligned to +/- strand): ref base T (2/3); new base C (2/2); total (4/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.91e-01 | |||||||||||
GAGATAATCTGGCAACCCTGAAAGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGG > CP009273/764218‑764458 | gagaTAATCTGGCAACCCTGAAAGGGGGATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTGCGCGTATGTGTATCAAAATTGGCCGCGCGCGTATTGAATATCAGATTGTTAATCTTCTGGAACCACTGGCa > 2:6235/1‑146 (MQ=255) cccTGAAAGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCCGGAACCGCCGGCAACACTGGCCTGGCCg > 2:235674/1‑146 (MQ=255) tttAGTGAGGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATa < 2:94607/146‑1 (MQ=255) aTTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACcaccac < 1:114164/150‑1 (MQ=255) aTGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGGCGTTGGGTTTTGAATACCACCACGGCt > 1:96884/1‑150 (MQ=255) tGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCCGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGctggctggga > 2:14580/1‑144 (MQ=255) caccGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCCGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGaaa < 1:24541/147‑1 (MQ=255) ttCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTaaaaaa < 1:28191/150‑1 (MQ=255) gCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCCGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCgg < 2:99343/146‑1 (MQ=255) | GAGATAATCTGGCAACCCTGAAAGGGGAATTTATTGATGGCAAATATATGCGCGTGCATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAATAAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATACCACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGTATCGG > CP009273/764218‑764458 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |