| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP009273 | 3,178,277 | G→T | 42.1% | E27* (GAA→TAA) | yqiC → | BMFP family putative fusogenic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP009273 | 3,178,277 | 0 | G | T | 42.1% | 13.2 / 17.4 | 22 | E27* (GAA→TAA) | yqiC | BMFP family putative fusogenic protein |
| Reads supporting (aligned to +/- strand): ref base G (6/6); new base T (4/5); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTT > CP009273/3178129‑3178405 | atggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGa > 1:15850‑M2/126‑150 (MQ=255) gTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAg > 2:232811/1‑146 (MQ=255) aacggtctaaataggctgattcaaagcatgtacggaagaaaaaatcggctcaaagatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGgag > 1:145736‑M2/116‑150 (MQ=255) cggtctaaataggcagattcaaggcatttacgggagcatcaatagggtcaaccatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGaa < 1:115206‑M2/37‑1 (MQ=255) aataggctgattcaaggcatttacgggagaaaaaatcgactcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGaaaaa < 2:80597‑M2/40‑1 (MQ=255) gctgattcaaggcattgacgggagaaaaaatcggcccaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATcc < 2:89178‑M2/45‑1 (MQ=255) gccgaatcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCa < 1:78954‑M2/49‑1 (MQ=255) caaggcatttacgggagaaaaaatcagctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGATAAAAATCCGCCAaacccgt > 1:195446‑M2/95‑148 (MQ=255) aacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCCGGGAGTTCGGGGGAGATGTGGAGACAAAAATCCGCCAAACCCTACAAGCGCAg > 1:184651‑M2/86‑150 (MQ=255) tCAACACCTATTAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGc < 1:223154/150‑1 (MQ=255) atcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCg < 1:84019‑M2/78‑1 (MQ=255) aCAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGAcc < 1:82285/150‑1 (MQ=255) cAGGGAAACTACAATGATTGACCCGTAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCAAAACCCTACAAGCGCAGCTGACGCGCCTCGACCt < 1:167719/150‑1 (MQ=255) gAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCt < 2:67231/146‑1 (MQ=255) aTTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAACAATCCGCCAACCCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAGGCCGTGa > 2:69820/1‑146 (MQ=255) aaaaaaTTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGAAATCCGGAAGTTCGGGAAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTcc > 1:117313/1‑150 (MQ=255) ccAGGTTCTCGAAGCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAACCCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTAc < 2:174557/146‑1 (MQ=255) cgcttattcgccccttccCTAAAGGAATGAGGGAGTTCGGGGAAGATGAGCAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGTCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACgg < 2:127249‑M2/128‑1 (MQ=255) ttcgcaccttccCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGaaa > 1:208442‑M2/13‑150 (MQ=255) cTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCACCTCGACCTTGTAACCCGTGAAGAGTTCGACGTCCAAACGCCAGTCCTGTTACGGACCCGGGAACACCTGgcc > 2:98758/1‑145 (MQ=255) aaaGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAATCCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCtt > 1:38238/1‑150 (MQ=255) aaaGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAAGGCAAGTCCTGTTACGGAGTCGTGAAAAAATGGCgct > 2:54412/1‑146 (MQ=255) | ATTCTGGTTTATGACTTACCCTTATCGCACTACAATGGCACTCAACACCTATCAGTACAGGGAAACTACAATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTT > CP009273/3178129‑3178405 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |