mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.32.0b
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | A6_F017_R1_F14_I30_S2156_L001_R1_001.good.fq | 1,498,977 | 223,347,573 | 100.0% | 149.0 bases | 149 bases | 96.4% |
| errors | A6_F017_R1_F14_I30_S2156_L001_R2_001.good.fq | 1,396,501 | 199,699,643 | 100.0% | 143.0 bases | 143 bases | 95.8% |
| total | 2,895,478 | 423,047,216 | 100.0% | 146.1 bases | 149 bases | 96.1% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | CP009273 | 4,631,469 | 87.3 | 1.7 | 100.0% | Escherichia coli BW25113, complete genome. |
| total | 4,631,469 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 30947 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 552 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.036 |
| reference sequence | pr(no read start) |
|---|---|
| CP009273 | 0.77403 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.8 |
| Consensus minimum coverage each strand | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum coverage each strand | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.2 |
| R | 3.4.2 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 18:11:26 15 Jun 2018 | 18:12:03 15 Jun 2018 | 37 seconds |
| Read alignment to reference genome | 18:12:03 15 Jun 2018 | 18:15:06 15 Jun 2018 | 3 minutes 3 seconds |
| Preprocessing alignments for candidate junction identification | 18:15:06 15 Jun 2018 | 18:15:35 15 Jun 2018 | 29 seconds |
| Preliminary analysis of coverage distribution | 18:15:35 15 Jun 2018 | 18:16:56 15 Jun 2018 | 1 minute 21 seconds |
| Identifying junction candidates | 18:16:56 15 Jun 2018 | 18:17:04 15 Jun 2018 | 8 seconds |
| Re-alignment to junction candidates | 18:17:04 15 Jun 2018 | 18:17:46 15 Jun 2018 | 42 seconds |
| Resolving alignments with junction candidates | 18:17:46 15 Jun 2018 | 18:18:34 15 Jun 2018 | 48 seconds |
| Creating BAM files | 18:18:34 15 Jun 2018 | 18:19:44 15 Jun 2018 | 1 minute 10 seconds |
| Tabulating error counts | 18:19:44 15 Jun 2018 | 18:20:18 15 Jun 2018 | 34 seconds |
| Re-calibrating base error rates | 18:20:18 15 Jun 2018 | 18:20:19 15 Jun 2018 | 1 second |
| Examining read alignment evidence | 18:20:19 15 Jun 2018 | 18:27:26 15 Jun 2018 | 7 minutes 7 seconds |
| Polymorphism statistics | 18:27:26 15 Jun 2018 | 18:27:27 15 Jun 2018 | 1 second |
| Output | 18:27:27 15 Jun 2018 | 18:27:40 15 Jun 2018 | 13 seconds |
| Total | 16 minutes 14 seconds | ||
