BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	4,546,084	G→T	H18Q (CAC→CAA)	yjiC ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,546,084	0	G	T	98.1%	353.2 / NA	107	H18Q (CAC→CAA)	yjiC	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (0/2); new base T (48/56); total (48/59)
Fisher's exact test for biased strand distribution p-value = 5.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

TTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCAT  >  CP009273/4545940‑4546228

ttttCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGAGGAAGGAATTGAg                                                                                                                                              >  2:509375/1‑149 (MQ=255)

 tttCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGc                                                                                                                                             <  1:1315878/149‑1 (MQ=255)

   tCTAATTTCGACAACGCTTTTTCCTTTTTTTTTTTAGTTATTGCCCAGAGGAAGAGGCGGACGATCGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTTTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCAt                                                                                                                                           >  1:1173121/1‑149 (MQ=255)

    cTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATc                                                                                                                                          >  2:1731959/1‑149 (MQ=255)

     tAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCt                                                                                                                                         <  2:912694/149‑1 (MQ=255)

       aTTTCGACAACGCTTTTTCCATCGTAATTGTGGTTATTGCCACGAGTAAGAGGGGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTACTGTACGTGTAAACTCACTGTCTACTATTGGGTGATGAAGGAATTGAGCATCTGt                                                                                                                                       >  1:971717/1‑149 (MQ=255)

       aTTGCGACAACGCGTTTTGCATCGTAATTATGGTTATTGCCACGAGGAAGAGGCGGAAGATCGAGGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGt                                                                                                                                       <  1:1390436/149‑1 (MQ=255)

         ttCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGtaa                                                                                                                                     <  2:1757842/149‑1 (MQ=255)

         ttCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGtaa                                                                                                                                     <  1:1547664/149‑1 (MQ=255)

            gACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGtaataa                                                                                                                                  >  1:88446/1‑149 (MQ=255)

             aCAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGCAGGAATTGAGCCTCTGTaataat                                                                                                                                 >  1:417150/1‑149 (MQ=255)

              cAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAACTTTCGTGTAAACTCTCTGTCGTCTATTGGGTGATGAAGGAATTGCGCATCTGTAATAAtt                                                                                                                                <  1:772620/149‑1 (MQ=255)

                 cGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGa                                                                                                                             >  2:635018/1‑149 (MQ=255)

                  gCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGCGCATCTGTAATAATTTggc                                                                                                                            >  2:290161/1‑147 (MQ=255)

                   cTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAACCTCACTGTCGACTGTTGGGTGATGAAGGAATTGAGCATCTGTAATAATTGGAct                                                                                                                           >  2:241663/1‑149 (MQ=255)

                   cTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGAct                                                                                                                           <  2:1734283/149‑1 (MQ=255)

                   cTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGAct                                                                                                                           <  2:1651327/149‑1 (MQ=255)

                    tttttCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGActc                                                                                                                          >  1:656564/1‑149 (MQ=255)

                    tttttCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGCAATAATTTGActc                                                                                                                          >  1:1480007/1‑149 (MQ=255)

                      gtcccATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTg                                                                                                                        <  1:1799172/146‑1 (MQ=255)

                          cATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTCGACTCTGAAgg                                                                                                                    >  2:895656/1‑149 (MQ=255)

                           aTCGTAATTATGGTTATTGCCACGAGTAAGAGTCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAggg                                                                                                                   >  1:943006/1‑149 (MQ=255)

                           aTCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAGTTGGGCATCTGTAATAATTTGACTCTGAAggg                                                                                                                   >  2:324/1‑149 (MQ=255)

                           aTCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAggg                                                                                                                   >  2:196760/1‑149 (MQ=255)

                             cGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCa                                                                                                                 <  1:1231614/149‑1 (MQ=255)

                               tAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCAtt                                                                                                               >  2:1548258/1‑149 (MQ=255)

                               tAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCAtt                                                                                                               >  1:1778558/1‑149 (MQ=255)

                                  ttATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCt                                                                                                            <  1:1201911/149‑1 (MQ=255)

                                   tATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCtt                                                                                                           <  1:1775808/149‑1 (MQ=255)

                                     tGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTaa                                                                                                         <  1:1060828/149‑1 (MQ=255)

                                       gTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTTGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATg                                                                                                       <  2:1512047/149‑1 (MQ=255)

                                       gTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTAGTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATg                                                                                                       >  2:600041/1‑149 (MQ=255)

                                       gTTATTGCCACGAGGAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATg                                                                                                       <  1:1444933/149‑1 (MQ=255)

                                        ttATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATgg                                                                                                      >  2:497687/1‑149 (MQ=255)

                                         tATTGCCACGAGGAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGGATCTTTAATAATTTGACTCTGAAGGGCATTGCTTAATGGc                                                                                                     <  1:307438/149‑1 (MQ=255)

                                          aTTTCCAGGAGTCAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAACCTCCCTGGCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCa                                                                                                    <  2:1173205/149‑1 (MQ=255)

                                           ttGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCAt                                                                                                   >  2:1473065/1‑149 (MQ=255)

                                             gCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAg                                                                                                 <  1:1774886/149‑1 (MQ=255)

                                               cACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGAc                                                                                               <  1:1021574/149‑1 (MQ=255)

                                                 cGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACat                                                                                             <  1:963669/149‑1 (MQ=255)

                                                     tAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACataata                                                                                         <  1:899294/149‑1 (MQ=255)

                                                     tAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACataata                                                                                         <  1:1599797/149‑1 (MQ=255)

                                                     tAAGAAGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACataata                                                                                         <  1:74833/149‑1 (MQ=255)

                                                      aaGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAg                                                                                        <  2:411898/149‑1 (MQ=255)

                                                       agagGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTTACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGt                                                                                       >  1:559727/1‑149 (MQ=255)

                                                        gagGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGtt                                                                                      >  1:1681224/1‑149 (MQ=255)

                                                        gagGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGtt                                                                                      >  1:599698/1‑149 (MQ=255)

                                                          ggCTGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTAc                                                                                    <  1:1310098/149‑1 (MQ=255)

                                                           gCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCAGTACTACCGTGGCGCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTAcc                                                                                   >  2:924376/1‑149 (MQ=255)

                                                             aaaaGATAAAGGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCtt                                                                                 <  1:271627/147‑1 (MQ=255)

                                                                agacagatATTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAACCTCACTGTCGACTAGTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTa                                                                              <  2:883179/149‑1 (MQ=255)

                                                                  atagatGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATg                                                                            <  1:248428/149‑1 (MQ=255)

                                                                  atagatGTTTTCCGAATCCATGCGTGCAGATTTTAATTTACGTGTAAACTCACTGGCGACTATTAGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGCCATAATAGTTACCTTTTATg                                                                            >  2:302887/1‑149 (MQ=255)

                                                                    agatGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAg                                                                          <  1:475710/149‑1 (MQ=255)

                                                                     gatGGTTTCCGAATCCATGCGGGCATATTTTAATTTACGTGGAAACTCACTGTCGTCTATTGGGTGATTAAGGAATTGAGCATCTGTAATAATTTGACACTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGt                                                                         <  1:783936/149‑1 (MQ=255)

                                                                           ttCCGAATCCATGCGTGCATATTGTTATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCa                                                                   <  1:1806195/149‑1 (MQ=255)

                                                                             ccGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGATATAATAGTCACCTTTTATGAGTATTTCACt                                                                 <  2:1014017/149‑1 (MQ=255)

                                                                             ccGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACt                                                                 <  1:180443/149‑1 (MQ=255)

                                                                              cGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAGGGTATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGCCATCATAGTTACCTTTTATGAGTATTTCACTg                                                                >  2:379499/1‑149 (MQ=255)

                                                                               gtctCCATGCGTGCATATTTTACTGTCCGTGTAAACTCACTGTCGCCTATTGGGTGCTGAAGGAATTGAGCATCTGTAATAATTTGTCTCTGAAGGGCATTTCTTAATGGCATAGCCATAATAGTTACCTTTTATGAGTATTTCACTGa                                                               <  2:665733/146‑1 (MQ=255)

                                                                                   ccATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTAATGtt                                                           >  1:405562/1‑149 (MQ=255)

                                                                                     aTGCGTGCATATTGTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTa                                                         <  2:834317/149‑1 (MQ=255)

                                                                                      tGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAg                                                        <  2:236941/149‑1 (MQ=255)

                                                                                           gCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGaaaaa                                                   >  1:849516/1‑149 (MQ=255)

                                                                                             atatTTTAATTTGCGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGTATCTGGAAGTATGGGCCGAATGGGATGGGACTAATACTTACCTTTTATGCGTATTACGCCGTTTTTTAGAAAAATa                                                 <  1:1726552/149‑1 (MQ=255)

                                                                                             atatTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATa                                                 <  2:1808496/149‑1 (MQ=255)

                                                                                                ttttAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGAt                                              >  2:1722077/1‑149 (MQ=255)

                                                                                                 tttAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATa                                             >  2:1336906/1‑149 (MQ=255)

                                                                                                  ttAATTTACGTGTAAACTCACGGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGTCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATaa                                            <  2:1781501/149‑1 (MQ=255)

                                                                                                      tttACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAttt                                        >  1:791785/1‑149 (MQ=255)

                                                                                                      tttACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAttt                                        >  2:906728/1‑149 (MQ=255)

                                                                                                       ttACGTGTAAACTCCCTGTCGACTATTGTGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTAGTTCACTGATGTTTAGAAAAACAGATAAAtttt                                       >  1:996097/1‑149 (MQ=255)

                                                                                                        tACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAAttttt                                      <  2:1055399/149‑1 (MQ=255)

                                                                                                          cGTTTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAAtttatctatttttct                                    <  1:1384776/149‑15 (MQ=255)

                                                                                                          cGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGACCACCTGTAAGAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCt                                    <  2:884713/149‑1 (MQ=255)

                                                                                                           gtgtAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTg                                   >  1:1768909/1‑149 (MQ=255)

                                                                                                           gtgtAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTg                                   >  1:1768974/1‑149 (MQ=255)

                                                                                                             gtAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTgta                                 <  1:580744/149‑1 (MQ=255)

                                                                                                             gtAAACTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTgta                                 >  1:1515245/1‑149 (MQ=255)

                                                                                                                 aCTCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTaa                             >  2:1149220/1‑149 (MQ=255)

                                                                                                                   tCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTaaaa                           >  2:1856178/1‑149 (MQ=255)

                                                                                                                   tCACTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTGTATGAGTATTTCACTGATCTTTAGTAAAACAGATAAATTTTTCTGTAGTaaaa                           <  2:1067789/149‑1 (MQ=255)

                                                                                                                     aCTGTCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGCTAAATTTTTCTGTAGTAAAAag                         <  1:860005/149‑1 (MQ=255)

                                                                                                                       tGTCGCCTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACCCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAagag                       <  2:1533419/149‑1 (MQ=255)

                                                                                                                         tCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGaa                     <  1:1431770/149‑1 (MQ=255)

                                                                                                                         tCGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGaa                     >  2:1254802/1‑149 (MQ=255)

                                                                                                                          cGACTATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAg                    <  2:937234/149‑1 (MQ=255)

                                                                                                                            aCTATTGGGGGAGGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTa                  <  1:837421/149‑1 (MQ=255)

                                                                                                                              tATTGGGTGGTGAAGGAATTGGGCATCTGTAATAAATTGACTCTGAAGGGCATTGCTTAATTGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAACAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTaaa                <  1:555017/149‑1 (MQ=255)

                                                                                                                              tATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTaaa                >  1:1802968/1‑149 (MQ=255)

                                                                                                                              tATTGGGTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTaaa                <  1:752820/149‑1 (MQ=255)

                                                                                                                              tATTGGGTGATGAAGGAAATGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACAAAAAAAAAAAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTaaa                <  1:1628731/149‑1 (MQ=255)

                                                                                                                                   ggTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAACAAGAGAAGTAAACAAAt           >  2:322231/1‑149 (MQ=255)

                                                                                                                                   ggTGATGAAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAAt           >  2:1377765/1‑149 (MQ=255)

                                                                                                                                     tgatgaAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAACTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGa         >  2:591976/1‑149 (MQ=255)

                                                                                                                                       atgaAGGAATTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAca       <  2:656621/149‑1 (MQ=255)

                                                                                                                                         gaaggtatTGAGCATCTGTAACAATGTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTAACTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACTTAGGAcatg     <  1:1554414/149‑1 (MQ=255)

                                                                                                                                         gaaggaatTGAGCATCTGTAATAATTTGACTGTGAGGGGCAGTGCTTAATGGCATAGACATAATAGTTAACTTTTATGAGCATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatg     >  1:15986/1‑149 (MQ=255)

                                                                                                                                            ggaatTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTTCAATAGTGGAGAAATTTTTCTGTAGTAAAAAGAGCAGTAAACAAATGAcatgcat  >  2:545436/1‑149 (MQ=255)

                                                                                                                                            ggaatTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatgcat  >  2:1697122/1‑149 (MQ=255)

TTTTCTAATTTCGACAACGCTTTTTCCATCGTAATTATGGTTATTGCCACGAGTAAGAGGCGGAAGATAGATGTTTTCCGAATCCATGCGTGCATATTTTAATTTACGTGTAAACTCACTGTCGACTATTGGGTGATGAAGGAAGTGAGCATCTGTAATAATTTGACTCTGAAGGGCATTGCTTAATGGCATAGACATAATAGTTACCTTTTATGAGTATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCAT  >  CP009273/4545940‑4546228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`