Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,708,758 G→C 9.7% E614Q (GAA→CAA)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,7580GC 9.7% 131.8 / 4.3 58E614Q (GAA→CAA) rsxCSoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base G (32/18);  new base C (3/3);  total (36/22)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCG  >  NC_000913/1708483‑1709036
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                        
gTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTACAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCCCAAGATGGAACAGCAACAGGCTAATGCGGCACCAGAACAACAGGTCGACCCGCGCAAAGCCGCCGTCGACCCCGCCATTGCCCCCGCCAAAGCGCGCAAGCCGCAACACCAACAGGCGACAGCGGACCCAGAAAAACa                                                                                                                                                                                                                                                                                      >  2:980059/1‑280 (MQ=255)
  gCAATTAGTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAgg                                                                                                                                                                                                                                                                                    <  1:315247/280‑1 (MQ=255)
   cAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGt                                                                                                                                                                                                                                                                                   >  1:198356/1‑280 (MQ=255)
      ttattGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTCGAACAGCAACAGGGTAATGCGGAATCAGAAGAAAAGCTCGAt                                                                                                                                                                                                                                                                               >  1:425117/1‑281 (MQ=255)
        attGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATc                                                                                                                                                                                                                                                                              >  2:257577/1‑280 (MQ=255)
          ggcagcaCGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAt                                                                                                                                                                                                                                                                               <  1:696169/276‑1 (MQ=255)
                    gAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAgccg                                                                                                                                                                                                                                                                  >  1:907247/1‑280 (MQ=255)
                       gCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGACGAACAGGTCGATCCGCGCAAAGccgccg                                                                                                                                                                                                                                                               >  1:420876/1‑280 (MQ=255)
                         ccGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCCGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAACGCGAGCAAGCCCGAAAAGAAACAGGCAAATGCCGAGCCAGAAGCACCGGTCCAGTCGCGCaactggaccctg                                                                                                                                                                                                                                                             >  2:27070/1‑270 (MQ=21)
                            tAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAAAAGGTTGATCCGCGAAAAGCCGCCGTCGAAg                                                                                                                                                                                                                                                         >  1:365741/1‑281 (MQ=255)
                             aaaaCGCACGCCAAAGCGAAACAGGCAGGACCGCATCAAGCTAACGACGCAGCAACCGTGCCTGATCCACGTAAAACTGCCGTTGGAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                                                         <  1:855526/280‑1 (MQ=18)
                             aaaGCGCAAGCCAAGGCCAAACAGGGAAAACTGCAAGAAAATAACGACGCCGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTAGCGGCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGGTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAg                                                                                                                                                                                                                                                         <  1:1026704/280‑1 (MQ=18)
                              aaGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGc                                                                                                                                                                                                                                                        <  1:257577/280‑1 (MQ=255)
                                   cAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGCAAGCCGGCACACCAACAGGCGAATGCCGACCCAACACAACCGGCTGACCCGCGCAACGCCCCCGGCGAAGCCGCTa                                                                                                                                                                                                                                                   >  2:688130/1‑280 (MQ=18)
                                          tgtcGAAACAGTCACAACTGCAGCAAACTAACGCCGCATAAACCGTTGATAAACCACGAAAAACTGCCGTAAAGGAAGCGATCACCCGCGCAGAAACGCGCAAGCTGGAACAGCAACAGGCTAGTGCCGAACCAGAACAACAGGTCGATCCACGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCg                                                                                                                                                                                                                                            <  2:716376/277‑1 (MQ=17)
                                                              cagcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCTGTGCCAAAGCGCGCAAGCTGGAACAGAAACAAGCTAATGCGGAACCAGAAGAACAGGTCGATACGCGCAAAGCTGCAGTCGAAGTTGCCAGTGCCCGTCCCAATGCACGCAAGCAGGa                                                                                                                                                                                                                       >  1:312343/1‑281 (MQ=18)
                                                               agcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGCCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAtt                                                                                                                                                                                                                                                 >  1:7041/1‑254 (MQ=255)
                                                                     tataggacgCGGCAAGCCTTGATGGTGCACAGAAAACGGCCGTCGAGGAAGCCCTCACACCCGCCAAAGAGACCAAGCTGGCAGAGCAACAGGCTAATGCGGAAGCAGAACAACAGGCCGATCCGCGAAAAGCCGCCGTAGAAGCCGCTATTGCCCGGGACAACGCGGGCAAGCAGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                <  2:420876/276‑1 (MQ=14)
                                                                     cTAACGACGCAGCAACCGTTGCTTATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACACCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                <  1:772438/281‑1 (MQ=255)
                                                                     cTAACGACGCAGCAACCGTTGCTGATCCACGAAAAACAGCCGTTGAAGCAGCTAGCGCCCGCGCCAAAGCGCGCAAGCTGTAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCACGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                <  1:385625/281‑1 (MQ=255)
                                                                                   aCCGTTGGCGATCCACCTAAAACTGCCGGTGAAGCAGCTATCGCCCGCGCAAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCAGAACCAGAACAACAAGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGa                                                                                                                                                                                                  <  1:27070/281‑1 (MQ=255)
                                                                                                  cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCCAAGCGCGCAAGCCGGCACAGCAACAGGCTAATGCGGAGGCAGAAGAACAAGTCGATCCGCGCCAAGCCGCCGTCGCAGCCGCGATTGCCCGAGCCAAAGCCCGAAAACGGTAAACGCAAACGGCTAATGCCGAACAAGaaaaaacagt                                                                                                                                                                                   >  2:969228/1‑276 (MQ=11)
                                                                                                  cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGAACCGCGCAACGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCAGAGCCAGAAACACAggg                                                                                                                                                                                   >  1:182702/1‑280 (MQ=255)
                                                                                                       aaCTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCCGCAGTCGACGCCGCTATTGCCCGCGCCAAAGGACGCAAGCCGGAACAGCAACAGGCTAAATCCGAGCACGAAACATAGGTCGaac                                                                                                                                                                              >  1:811826/1‑279 (MQ=14)
                                                                                                            ccGTTGAAGCATCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCCAAGCCGCCGCCGAAGCCGCCATTGACCGCGCCAACCCACGCAAGCTGGAACAGAAAACGGCTAACGCGGAGACAGAAGAACAgtgttagccgcc                                                                                                                                                                          >  1:1006353/1‑269 (MQ=14)
                                                                                                             cGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGGGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCa                                                                                                                                                                                                                              >  1:45523/1‑227 (MQ=255)
                                                                                                                                       aaGCGCCCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAAAACAACCGGTCGATCCGCGCAAAGCAGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGc                                                                                                                                               <  1:457180/280‑1 (MQ=255)
                                                                                                                                                      aaCAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCCCGCAAGCCGGACCAGCAACAGGCTAATCCCGAGCCCGAACTACAGGTCGACACACGCAAAGCCGCCGTTGAAGCCCCCATTGCCCGCGCCaaacgccaa                                                                                                                               >  1:618768/1‑275 (MQ=14)
                                                                                                                                                                     aTGCAGAACCAGAACAACAGGTCGAGCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCaa                                                                                                                <  1:725732/281‑1 (MQ=255)
                                                                                                                                                                      tGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCACGTGCCAAAGCACCCAAGCCGGAACAGCAACAGGCTAATGCCGAGCCAGAAGCACAGGTCGATCCGCGCAAAGCCGCCGCCGAAGCCGCCATTGCACGAGCCAAAGCGACGAAACGGGCACCGCAAc                                                                                                               >  1:269236/1‑281 (MQ=14)
                                                                                                                                                                         ggAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaaca                                                                                                                                                                                         <  1:191387/204‑1 (MQ=255)
                                                                                                                                                                           aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAAAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGAGAACAGCAAACGGCTa                                                                                                         >  1:905356/1‑281 (MQ=255)
                                                                                                                                                                                    aacaGGTCGATCCGCGCGAAGCCGCCGTCGAACCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAGGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCcgcg                                                                                                                                                                          <  1:363586/208‑1 (MQ=255)
                                                                                                                                                                                           cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGGCGATCCGCGCAAAGCCGCCGTCGACGCGGCCATTGCCCGTGCCAAAGCCCGCCAGCGGGAACAGCAACAGGCTAATGCCGACCCCGAAAAAAACGGCGAACCATCCAAAGCCGTCGTCCAAGACGCAAATGCACGAGACCAAGCACGCAAACGGACCCAGAAAACGGCAAATGCggccgcacaca                                                                                          >  2:792835/1‑272 (MQ=14)
                                                                                                                                                                                              tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACAGGAACAGCAACAGGCTAATCCGGAGCCAgaagaa                                                                                        >  1:1065464/1‑280 (MQ=255)
                                                                                                                                                                                                            ataggagacaggCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGACCAGGTCGATCCGCGCGAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcgcg                                                                         <  1:505558/269‑1 (MQ=255)
                                                                                                                                                                                                                         tATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGAAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGATGCAGCAGAACAGGTTGATCCGCGCAAAGCTGCCGGCg                                                           >  1:964117/1‑281 (MQ=255)
                                                                                                                                                                                                                            ttcccGTGGCAAAGCGCGCAAGCTGAAACAGCAACAGGCTGATGCCGAACCAAAATAACAGGTCGATCCGCGCAAAGCCGCCTTCGAAGCCGCTATTGCCCGTGCCAACGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGACGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGa                                                          <  1:467717/277‑1 (MQ=14)
                                                                                                                                                                                                                             gccggagCCAAAGCGTCTAAGCAGCAACAGCAACAGGCCAAGGCGGACCCAGAAGATCAGGTCGATCCGCCCAAAGCCGCCGTCGAAGCCGCCATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAg                                                        <  1:606683/274‑1 (MQ=14)
                                                                                                                                                                                                                              cccGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACCGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACGGGAACAGCAACCGCATAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAACGCTGCAGTTCAAGc                                                       >  1:416115/1‑280 (MQ=255)
                                                                                                                                                                                                                                 gcgCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCa                                                                                                                                                                                                                                       <  2:376490/100‑1 (MQ=11)
                                                                                                                                                                                                                                  tGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCCGGAACAGACACCGGCTAATGCGTAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGCCGCAGCCGCCATTGCCCCCGCCCAAGCGCACCAACGGGCACCGCAAACGCATCACGCGGCACCAGAAACAAAGGGTGCACCGCCGCAAACCGCCGTCCCAGCGGcc                                                   >  2:477015/1‑279 (MQ=11)
                                                                                                                                                                                                                                                     gacccgCAACAGGCTAATCCGGAACCCGCAGCACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACg                              <  1:901786/276‑1 (MQ=255)
                                                                                                                                                                                                                                                       aCAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCa                            >  1:575525/1‑281 (MQ=255)
                                                                                                                                                                                                                                                           cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGTTAATGACGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGATCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGAATCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCACGTGCTCAACGCAGCAAGcc                        >  1:308398/1‑280 (MQ=14)
                                                                                                                                                                                                                                                           cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCCGAAAACCGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTCGAACCGCGCAAACCTGCCGTCAAAGCGGCCAATGCACGCGCCAAAGCACGaaatac                        >  1:657638/1‑278 (MQ=14)
                                                                                                                                                                                                                                                           cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGACGAGCCAGAACAACAGGTCGAGTCGCGCAAAGCCGCTGCCGACGCCGCTATTGCCCGAGCCCAAGCCGGCAAACGGGAAAAGCAAAAGGATAAAGCCGAGCCATAAGCACAGGTGTAATCGCGCAAAGCTGCCGTCGAAGCGGCTCTTGTCCGCGCCAAAgggagcaaacc                        >  2:854575/1‑271 (MQ=14)
                                                                                                                                                                                                                                                           cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCCATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCCAAGCCGCCGTCGCAGCCGCCCTTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAAAGCGGACCCCGAAGACCCGGTCGCACCGCCAAAACGTGACGCCGAAGCCGCCAACGCACCCACCCAAGCACGCAAgg                         >  2:100281/1‑279 (MQ=14)
                                                                                                                                                                                                                                                           cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAATCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTCCCCGCGCCAAAGCACGCAAGCt                        >  1:198319/1‑281 (MQ=255)
                                                                                                                                                                                                                                                               aGGCTAATGCGGAACCAGAACAGCTGGTCGATCCTCGCAAAGGCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGaa                    <  1:688130/281‑1 (MQ=255)
                                                                                                                                                                                                                                                                ggCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCGGCTATTGCAAGCGCCAAAGCACGAAAggcggcac                   >  1:233079/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                     atacGGAACCAGAACACCCGGCCGATCCGGCCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCAAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa              <  1:948288/278‑1 (MQ=255)
                                                                                                                                                                                                                                                                     aTGCGGAACCAGAACAACCGGTCGATCCCCGCAAACACGCCGTCGAACCCGCTATTGCCCGGGCAAAAGCACGCAAGCTGGAAAAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa              <  1:1068975/281‑1 (MQ=255)
                                                                                                                                                                                                                                                                           aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGAGGAGCCAGAAGAACAGGTGGATCCGCGCAAAGCTGCCGTAGAAGCGGCTATTGCACGCGCCAAAGGACGCAAGCTGGAACAGCAAAAggat        >  1:308859/1‑279 (MQ=255)
                                                                                                                                                                                                                                                                           aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCAGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAggac        >  1:1066052/1‑279 (MQ=255)
                                                                                                                                                                                                                                                                           aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGAGCAAAGCTGCCGTCGACGCGGCTATTGCCAGCGCCAAAGCACGCAAACTGGACCAGACACCGGCt        >  1:769656/1‑281 (MQ=255)
                                                                                                                                                                                                                                                                           aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGGTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCCGGAACAGCAACAGGCt        >  1:980583/1‑281 (MQ=255)
                                                                                                                                                                                                                                                                           aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTAATGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCt        >  1:841405/1‑281 (MQ=255)
                                                                                                                                                                                                                                                                                 aagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACCGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCCCCAAACGGGAACAGCAACCGGCTCAAGCGGAGCCAGAAGAACAGGTTGATCCGCCCCAAGCTGCCGCCGAACCGGCGATTGCACGCCCCACAGCCCGCAATCTGGAACAGCAAACGGCTaactcg  >  2:1118757/1‑277 (MQ=17)
                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                        
GTGCAATTATTGCAGCACGGGAAGCCCGTAAAGCGCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCG  >  NC_000913/1708483‑1709036

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: