Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,173,742 Δ1 bp 5.7% pseudogene (537/914 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,7420A. 5.7% 209.8 / 4.7 70pseudogene (537/914 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base A (34/28);  new base . (2/2);  total (39/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01

GTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACGGGTCAGTAACATCGCCACGTTAACCAGAATCGCAATCGGAATCGCCACCAGCGCAATTTGCGACGCCCAGGTCATTGG  >  NC_000913/2173483‑2174017
                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                   
gTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCCCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCATGGGATTTTCTCGATGATAGCATCGACCAACACCGCAATCGGCCCCATATCCGACGCCGTCCCGTGCGGACTAGCAAAACCTTCCCGCTCa                                                                                                                                                                                                                                                                >  2:844577/1‑281 (MQ=255)
 tttAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCa                                                                                                                                                                                                                                                                >  1:187470/1‑280 (MQ=255)
 tttAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACAAAATTTGCGCTGAATATCGTCGGCGCTAAATCTAATTCGGTTTACGCCTGGGATTATCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATATGCCTAAGTAACGTGCGGCATAGCAAttgtcctttatacac                                                                                                                                                                                                                                                               >  1:271086/1‑267 (MQ=255)
   tAATCACCCGTGGCGTTAGCAACATCACGGCCGCCGTTTTGACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAg                                                                                                                                                                                                                                                             <  2:41947/281‑1 (MQ=255)
         cccTTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCaa                                                                                                                                                                                                                                                                              <  1:488805/258‑1 (MQ=255)
           cGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAACTGGACCAAATTTGCGCTGAATATCGTCGGCGCTCAATTTATTTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAAACGGCCCCATCTCCCCCGACCTACCGTGCGGAATAGCAAAACCTTCCAGcctgtctcttatac                                                                                                                                                                                                                                                      >  2:488805/1‑267 (MQ=255)
                         caTCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTATCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCCCAAGAAAATGTCGGGTATCGAgggga                                                                                                                                                                                                                                        >  1:891813/1‑278 (MQ=255)
                              cTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAg                                                                                                                                                                                                                                  >  1:170183/1‑281 (MQ=255)
                                   gccgTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCAGCGCCAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGAACAACACCGCAATCGGCCCCATATACGCCGACGTACCGTACGGCATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGACAACCAGTTGcc                                                                                                                                                                                                                             >  1:1062400/1‑281 (MQ=255)
                                    ccgTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCg                                                                                                                                                                                                                            >  2:1125036/1‑281 (MQ=255)
                                               gCCATCTACAATACCCCCTTAACCTCGTAACCCGCGAGGATGCCGATAATCACCCCCATCCCAAAACCCACGTTGACGGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATaaac                                                                                                                                                                                                                 <  2:170183/281‑1 (MQ=255)
                                                 cAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATCGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGCGCTTATcaacaa                                                                                                                                                                                                               >  1:602834/1‑281 (MQ=255)
                                                               cTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCCTCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGCATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAAAGCCGCGTGAAt                                                                                                                                                                                                  >  1:891088/1‑280 (MQ=255)
                                                                        cGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAAcg                                                                                                                                                                                                            <  2:59938/261‑1 (MQ=255)
                                                                        cGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAAcg                                                                                                                                                                                                            >  1:59938/1‑261 (MQ=255)
                                                                         gTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCCGAATATCGTCGGCGCTAAATTTAATTCCGTTAACGCCTGGGATTTTCCCGATGATAGCATCGACCAGCACCGCAATCGGCCGCCTACACGTCGACGACCCGTGCGGAATAGCCATACCTTCCCGCCCCAAGAAATTTCGGGCATCCCGGGCAACCCCGTCGCCGAGCTCATCAACAACACCCGCGTGAACCACCACAcccg                                                                                                                                                                                       >  2:179017/1‑279 (MQ=255)
                                                                             cccGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCa                                                                                                                                                                                    <  1:1125036/280‑1 (MQ=255)
                                                                                 cgAGGATGCCGATACTCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGAACAAATTTGCGCTGAATATCGTCGGCACTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGAGGATAGACTCGACCAGCACCGCAATCGGCCCCATATACGCCTACGTACCGTGCGGAATCGCAAAACCTTCCAGCCAACAGAAATTTCAGGTATCGCGGGCTAAACAGTCGCCGATCTTATAAAAAAAAACCGCGTGAAGTCACACACTCTCCATccc                                                                                                                                                                                >  2:18556/1‑280 (MQ=255)
                                                                                    cagttccGATAACCAGCCCCATCACCAACCCCGCGGTGACAGGCTCGCCACATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTatc                                                                                                                                                                            <  2:403930/276‑1 (MQ=255)
                                                                                                  aGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTaccat                                                                                                                                                                          >  1:403930/1‑269 (MQ=255)
                                                                                                         tCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAg                                                                                                                                                        >  1:1073842/1‑280 (MQ=255)
                                                                                                         accaaaaaCCCCCGGTGACCGGCTCGCCAAATGGCCCAAATTTACTCTGAATGTGATCAGCGGTAAATTTAATTCGGTTACCGGCGGGGATTTTCTCAATGATAGGAGCGACCAGCACCGGAATCGGCCCCATATACGCCGACGAACCGTGCGGAATAGCAATACCTTCCAGCTCAAGGAAATTTCGGGTATCGCGGGCAAGACAGTTGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGa                                                                                                                                                       <  2:891088/277‑1 (MQ=255)
                                                                                                              aaaCCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTAAAAACACACGCCGCCTGAACTACCACACCTGCCATCCCCATCCTCCACAACCCGGTTGCCCCATgca                                                                                                                                                   >  2:796480/1‑280 (MQ=255)
                                                                                                                    aCGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGTTGATAGCATCGACCAGCACCGCAATCCGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCAAACCGGCCACCCCAATCATCCACTAACCCGCTCCCAGATgcagccatgc                                                                                                                                            >  1:747133/1‑276 (MQ=255)
                                                                                                                     cGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCCAGCTTAAAAACAAACGCCGCGTGAATTACCACAACTGCCATCCCCATCATCCATGAACCGGTTGCCAGATGCAGCAACgagc                                                                                                                                          >  2:654958/1‑281 (MQ=255)
                                                                                                                     cGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGATAGCAATACCTTCCAGCACAAAGAAATTTCGGGTACCGCGGGCAAACCAGTCGCCCAGCCGAACAACAAACGCCGAGTGAATCCAGACACCTGCCAGCCTTACCATCCATGACCCGGTTGCCAAATGCAGCAAcgccc                                                                                                                                          >  2:963596/1‑279 (MQ=255)
                                                                                                                      ggTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCAATCCCTATCATCCATGAACCGGTTTCCAGATGCAGCAAcgccc                                                                                                                                          >  2:829994/1‑279 (MQ=255)
                                                                                                                           cAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCg                                                                                                                                                                                                                            >  2:525193/1‑194 (MQ=255)
                                                                                                                           cAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCg                                                                                                                                                                                                                            <  1:525193/194‑1 (MQ=255)
                                                                                                                                  gCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCCCACCTGCCATCCCTATCATCAATGAACCGGTTGCCAGAGGCAGCAACCCGCCGGGGAAGGTCa                                                                                                                              >  2:423836/1‑281 (MQ=255)
                                                                                                                                  gCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGc                                                                                                                                                                                      <  2:862364/225‑1 (MQ=255)
                                                                                                                                  gCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGc                                                                                                                                                                                      >  1:862364/1‑225 (MQ=255)
                                                                                                                                       aTGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGGGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCatat                                                                                                                           <  1:608417/279‑1 (MQ=255)
                                                                                                                                       aTGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCAATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCa                                                                                                                              <  1:423836/276‑1 (MQ=255)
                                                                                                                                        tGGACCAAATTTGCGCTGGATTTCGTCGGCGCCAAATTTAATTCGTTTAACGCCTCGGATTTTCTCGATGATAGAATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTTTCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTACCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGcc                                                                                                                        <  1:963596/280‑1 (MQ=255)
                                                                                                                                        tGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGcc                                                                                                                        <  1:654958/280‑1 (MQ=255)
                                                                                                                                          gACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCGTCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTAc                                                                                                                                                                                               <  1:660238/208‑1 (MQ=255)
                                                                                                                                           aCCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACCCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGc                                                                                                                                                           <  1:699313/243‑1 (MQ=255)
                                                                                                                                            ccAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGa                                                                                                                     <  1:829994/280‑1 (MQ=255)
                                                                                                                                                          aaTATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCatat                                                                                                                           >  1:1212231/1‑260 (MQ=255)
                                                                                                                                                          aaTATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCatat                                                                                                                           <  2:1212231/260‑1 (MQ=255)
                                                                                                                                                          aaTATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCa                                                                                                       >  1:57992/1‑280 (MQ=255)
                                                                                                                                                                    gcgcTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTTCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTCAAGGTCATATTCCAGATATTCCAGATATCAACATTTaccac                                                                                            >  1:60322/1‑281 (MQ=255)
                                                                                                                                                                    gcgcTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTaccac                                                                                            <  1:1052541/281‑1 (MQ=255)
                                                                                                                                                                    gcgcTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGAATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCcgcg                                                                                                                                                                                                       <  1:201136/174‑1 (MQ=255)
                                                                                                                                                                    gcgcTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGAATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCcgcg                                                                                                                                                                                                       >  2:201136/1‑174 (MQ=255)
                                                                                                                                                                    gcgcTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGAGGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTaccac                                                                                            <  1:844577/281‑1 (MQ=255)
                                                                                                                                                                    gcgcCAAATTTAATTCGGTTAACGCCTGCGATGTTCTCGATGATAGCATCGGCCAGCACCGCAATCGGCCCCATATACGCCGACGTACCTTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTaccac                                                                                            <  2:57992/281‑1 (MQ=255)
                                                                                                                                                                               aaTTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCCCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAAAGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCTAGCTATTCCAGATATCAAAATTTACCCCCCGCGTCAtcc                                                                                 >  2:898643/1‑279 (MQ=255)
                                                                                                                                                                               aaTTCGGTTAACGCCTGGGATTTTCTCGATGAAAGCATCTACCAGCACCGCAAACGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATa                                                                                  <  1:859599/280‑1 (MQ=255)
                                                                                                                                                                                      ttAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATAGCAACATTTACCACCCGCGTCATACGGGTCa                                                                           >  1:1199024/1‑280 (MQ=255)
                                                                                                                                                                                              tGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACGGGTCAGTAACATCg                                                                  <  2:1073842/281‑1 (MQ=255)
                                                                                                                                                                                               gggATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACGGGTCAGTAACactg                                                                  >  1:193676/1‑277 (MQ=255)
                                                                                                                                                                                                ggATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTTCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGTTTGCCAGATTCAGCCACGCGCCGGTGAAGGTCATATTCCAGATATTCCAGATATCAACATTTACCACCCGCGGCAGTCGGGTCAGTAACATCGc                                                                 >  1:1205579/1‑280 (MQ=255)
                                                                                                                                                                                                           atggtAGCAGCGACCAGCACCCCAATCGGCCCCACATACGCCGACGGACCGGGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGGTGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACGGGTCAGTAACATCGCCACGTTAACCa                                                      <  2:115402/280‑1 (MQ=255)
                                                                                                                                                                                                               tAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATTAACCGGTTGCCAGATGCAGCAATCCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACGGGTCCGTAACCTCGCAACGTTAACCAGCATc                                                 >  1:72066/1‑281 (MQ=255)
                                                                                                                                                                                                                                ataagggacagcATAGACGCCAACGTACCATGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCAGGTATCGCGGGCAAGCCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACGGGTCAGTAACATCGCCACGTTAACCAGAATCGCAATCGGAATCGcca                                 <  1:1090497/269‑1 (MQ=255)
                                                                                                                                                                                                                                 cAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGGTGCAGCAACGCGCCGGTGCAGGTCATATGCCAGATATTCCAGATACCAAAATTTACCATCCGCGTAATACGGGTCAGTAACATCCCCACGTTAACCAGACACGCAATCGGAACCGcccac                               >  1:111949/1‑278 (MQ=255)
                                                                                                                                                                                                                                 cAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACg                                                                                >  1:638599/1‑232 (MQ=255)
                                                                                                                                                                                                                                            atatACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACGGGTCAGTAACATCGCCACGTTAACCAGAATCGCAATCGGAATCGCCACCAGCGCAATTTg                    <  1:796480/281‑1 (MQ=255)
                                                                                                                                                                                                                                               taCGCCAACGTACCGTTCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGGCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACGGGTCAGTAACATCGCCACGTTAACCAGAATCGCAAGCGGAATCGCCACCAGCGCAATTTGCg                  <  1:277224/280‑1 (MQ=255)
                                                                                                                                                                                                                                                   cccaCGGACCTTGCGGTATCGCAATGCCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGCACTTATAAACCAGCGCCGCGTGCATTACCACACCTTCCGTCCCTATCATCCATGAACCGGTTGCCGGATGCAGCAACGCTCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTTCCACCCGCGTCATACGGGTCAGTAACATCGCCACGTTAACCAGAATCGCAATCGGAATCGCCACCAGCGCAATTTGCGACGcc             <  2:168530/278‑1 (MQ=255)
                                                                                                                                                                                                                                                   ccGACGTACCGAGCGGAATAGACATACCTTCCAGCTCAACGAAATTTCGCGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTAGCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACGGGTCAGTAACATCGCCACGTTAACCAGAATCGCAATCGGAATCGCCACCAGCGCAATTTGCGACGcc             <  2:602834/281‑1 (MQ=255)
                                                                                                                                                                                                                                                     gACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACACGACATCCCTATCATCCCTGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATCTGCCCGCTATTCCAGATATCAACATTTACCACTCGCGTCATACGGGTCAGTAACATCGCCACGTTAACCAAAAACGCAACCCGAACTGCCACCACCCCCATTTGCGCAGccc            >  2:277224/1‑280 (MQ=255)
                                                                                                                                                                                                                                                       cGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTaccac                                                                                            <  2:1105246/198‑1 (MQ=255)
                                                                                                                                                                                                                                                       cGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTaccac                                                                                            >  1:1105246/1‑198 (MQ=255)
                                                                                                                                                                                                                                                       cGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACGGTTCAGTAACCTCGCCCAGTTAACCAGAATCGCAATCTGAATCGCCACCATCGCAATTTGCGCCCCCCAgg         >  2:1002283/1‑281 (MQ=255)
                                                                                                                                                                                                                                                       cGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACGGGTCAGTAACATCGCCACGTTAACCAGAATCGCAACCGGAATCGCCACCAGGCCAATTTGCGCACCCCAgg         >  2:283782/1‑281 (MQ=255)
                                                                                                                                                                                                                                                           ccGGGCGGAAAAACAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTAGAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGCACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACGGGTCAGTAACATCGCCACGTTAACCAGAATCGCAATCGGAATCGCCACCAGCGCAATTTGCGACGCCCAGGTCa      <  2:1199024/280‑1 (MQ=255)
                                                                                                                                                                                                                                                              tGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACGGGTCAGTAACATCGCCACGTTAACCAGAATCGCAATCGGCATCGCCACCAGCGCAATTTGCGACGCCCAGGTCATTgc  >  1:168530/1‑280 (MQ=255)
                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                   
GTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGCTGAATATCGTCGGCGCTAAATTTAATTCGGTTAACGCCTGGGATTTTCTCGATGATAGCATCGACCAGCACCGCAATCGGCCCCATATACGCCGACGTACCGTGCGGAATAGCAATACCTTCCAGCTCAAAGAAATTTCGGGTATCGCGGGCAAACCAGTCGCCGAGCTTATAAACAAACGCCGCGTGAATTACCACACCTGCCATCCCTATCATCCATGAACCGGTTGCCAGATGCAGCAACGCGCCGGTGAAGGTCATATGCCAGATATTCCAGATATCAACATTTACCACCCGCGTCATACGGGTCAGTAACATCGCCACGTTAACCAGAATCGCAATCGGAATCGCCACCAGCGCAATTTGCGACGCCCAGGTCATTGG  >  NC_000913/2173483‑2174017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: