Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,123,175	C→T	6.1%	T55T (ACG→ACA)	ftsN ←	essential cell division protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,123,175	0	C	T	6.1%	258.6 / 4.5	93	T55T (ACG→ACA)	ftsN	essential cell division protein
Reads supporting (aligned to +/- strand):  ref base C (39/45);  new base T (3/3);  total (43/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.03e-01

CTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCC  >  NC_000913/4122896‑4123456                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                           gtGTTCCGGCGTCTGTTCCTTCCACGGAACTTCAGCCAGCTTCGTTGGCTGTTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGGTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCg                                                                                                                                                                                                                                                                                           <  2:247605/280‑1 (MQ=255) cTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCg                                                                                                                                                                                                                                                                                           <  1:558247/281‑1 (MQ=255)  tGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCg                                                                                                                                                                                                                                                                                           <  2:981315/280‑1 (MQ=255)  tGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCg                                                                                                                                                                                                                                                                                           >  1:981315/1‑280 (MQ=255)    ttCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGGGTTGGCTGCTGGGGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACGTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTa                                                                                                                                                                                                                                                                                         <  2:1058048/280‑1 (MQ=255)    ttCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCCTCTCGGACTCTTCTTTCTTGTGATGCGTaa                                                                                                                                                                                                                                                                                        >  1:483137/1‑281 (MQ=255)       cGGCGTGTTGTCATTCCACGGGAAGTCAACCAGCTGGGTGGGCTGCTGGCGCATATCGGCCTGCATTTGTTACAGAAGCTGACGTTGTTCTGGTGTCAGTGGCTCCGGCTTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCGCTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCGTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATg                                                                                                                                                                                                                                                                                      <  2:1029349/280‑1 (MQ=255)        ggcggcTGTTCATTCCCCGGAACTTCAACCGGCGGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATg                                                                                                                                                                                                                                                                                      <  1:983396/279‑1 (MQ=255)              tGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCCCTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCGGCGTGCTTCTGGGTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCGCGGACGCTGCTTTCTTGTGATGCGTAATTAAGTACa                                                                                                                                                                                                                                                                               >  2:983396/1‑280 (MQ=255)                ttCATTCCGCGGAACTTCAGCTAGCTGTTTTGGCTGGTGGGGCACATCAGACTGCTGTTGTTCAAGAAGCTGACGTTGGTCTGGTGTCGGTTGCGCCGGCGTTAGCACTTCGCCACGGGCAGAAGGTTCTG‑TGCGCGTACGCACTCCCGGCTGGCGACTTTCCAGCTCGTTGATGT‑AGCGCGAGCGTTTTTCTGGTTTTGGGGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGTGTAATGAAGTACAGac                                                                                                                                                                                                                                                                            <  2:763970/281‑1 (MQ=255)                  cATTCCACGGAAGTTCAAACAGCTGCGTTGGCTGCTGGCGCAGATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGacc                                                                                                                                                                                                                                                                           <  1:49754/280‑1 (MQ=255)                            gaCTTCGACGAGCTTCGGTGGCTGTTGGCGCAGTGCAGCGTGGGTTTTTTCAGGGGACTGTCGTTGTTCGTGTGTGATTTGCTGTGGCGTTTTCGATTCACCGCCGGC‑GAAGGGTTTGATGGGCGACCGGGCTCCCGGCTTGCGACTTTCCAGCTCTGTGATGT‑GGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGTTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTAATGCGTAATGAGGGACAGACCACCGATAAAg                                                                                                                                                                                                                                                                 <  2:264851/279‑1 (MQ=255)                                 cAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGGTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACGCTTCTTTCTCGTGATGGGTAATGAAGTCAACACCACCGATAAAGGTcaca                                                                                                                                                                                                                                                           >  2:13565/1‑281 (MQ=255)                                         tGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTCCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAAcggc                                                                                                                                                                                                                                                   <  1:139786/281‑1 (MQ=255)                                           cGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAAcggcgg                                                                                                                                                                                                                                                 <  1:264886/281‑1 (MQ=255)                                                   gctgGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATa                                                                                                                                                                                                                                         >  1:1026083/1‑281 (MQ=255)                                                     tgGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGacc                                                                                                                                                                                                                                                                           >  1:383595/1‑245 (MQ=255)                                                     tgGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGacc                                                                                                                                                                                                                                                                           <  2:383595/245‑1 (MQ=255)                                                     tgGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTGTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTCCAGACCACAGATAAAGGTCACAAGAACGGCGGCCGCAATAg                                                                                                                                                                                                                                        >  1:558238/1‑280 (MQ=255)                                                            tatCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGacc                                                                                                                                                                                                                                                                           >  2:580963/1‑238 (MQ=255)                                                            tatCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGacc                                                                                                                                                                                                                                                                           <  1:580963/238‑1 (MQ=255)                                                                  ccTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTGTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGTCTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGCTAAAGGTCGCAAGAACGGCGGCAGCAAAACGGACCATAGCggg                                                                                                                                                                                                                           >  2:371393/1‑280 (MQ=255)                                                                     gttttttgtaaagcagCTGAGGGTGTTGTGGTGTTGGTTGCTCCGGGGTTTTCATTTCACCACCCGCGGAAGGTTCTG‑GGGGCGCACGCACTCCCGGGTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTTGTGGTATTCCGGTTCCGGTCACTTTCTGGCTGTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGgaga                                                                                                                                                                                                                        <  2:885595/270‑1 (MQ=255)                                                                      cATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTCCTGGTGTTGGTGGTAGTCCGTTCCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACCACTCTTTCTTGTGATGCGTAATGAAGTACAGACAACCGATAAAGGTACCAAAAACGGCGGCAGCAATAGCGCCCAGAGCGGGAGaaa                                                                                                                                                                                                                      >  2:452451/1‑281 (MQ=255)                                                                            ttCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCgca                                                                                                                                                                                                                 >  2:478903/1‑280 (MQ=255)                                                                                     cTGACGGTGTTTTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCATCGTTATTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTc                                                                                                                                                                                                       <  2:916712/281‑1 (MQ=255)                                                                                       gACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGt                                                                                                                                                                                                     <  1:478903/281‑1 (MQ=255)                                                                                                        aGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGTCGACTTTCCAGCTCTTTCATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGT‑ATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGCTTTCGTTGCTTTTTCCGTGAGGTg                                                                                                                                                                                   >  1:1018802/1‑281 (MQ=255)                                                                                                        aGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCTGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACATGAACGGCGGCAGCAATAGCGACAATAGCGGGAGAAACTGCAGGCAGATTTCGTTGTGTTTTCCGCGAGGt                                                                                                                                                                                    >  2:410323/1‑281 (MQ=255)                                                                                                                  gCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACGCTTCTTTCTTGTGATTCGTAATGAAGTACAGACCACCGATAAAGGTCAAAAGAACGGCGGCCGCAATAGCCACCATAGCGGGAGAAACCGTCGGCAGCGTTGGGTGCTTTTTCAGGGGGGGGCCAtttttt                                                                                                                                                                          >  2:374501/1‑281 (MQ=255)                                                                                                                          agttcgcCGCCGGCAGGAGGTTGTA‑GGGGCGCTCAGAATCCCGGATGAGCACTTTCAGGCTCTTGAAGTT‑GGGGCCGACGTTCTTTGGGTGTTGGGGGTAGTCCGGTTGCGGTCACTTTCGGGCTTGGCAGCGTCTCGGACTGTGCTTTCTTGTGATGAGTAATGAAGTACAGACCACCGATAGAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCcgcg                                                                                                                                                                  <  2:61671/278‑1 (MQ=255)                                                                                                                           ctgcccccccGGGAGCAGGTTGTG‑TGGTGGAACGCACTGCCGGCTGGCGACGTTCCAGCTCTGTAATGT‑AGCGACGGCGTTTTTCTGGTTTGTGTGGTAGTCCGTTTCCGGTCACTTTCTGACTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGa                                                                                                                                                                 <  2:746227/272‑1 (MQ=255)                                                                                                                               accaccGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTg                                                                                                                                                            >  1:360071/1‑281 (MQ=255)                                                                                                                                    cGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCCGATTTCGATGCTTTTTTCGTGAGGTGCTCTTTTTTG‑GCCGCGAAGGTGCCGGt                                                                                                                                                       >  1:326681/1‑281 (MQ=255)                                                                                                                                          aaGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTTGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTg                                                                                                                                                 <  1:695723/281‑1 (MQ=255)                                                                                                                                            ggTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGGCTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCTGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACATGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTgcg                                                                                                                                               <  1:410323/281‑1 (MQ=255)                                                                                                                                                cTG‑TGCGCGCGCACCCTCCCTGCTCGCGACTTTCCAGC‑GTTTGACGTCAGCGCCACCTTTTTTCTGGTTTTGTTGGACTTCCGTTTCCGGTCAGTTGCGGGCTGTGCAGGGTCTCGGACTCTGCTTTCTTGTTGTGCGTAATGAGGGACAAACCACCGATAAAGGGCACAAGAACGGCGGCAGCAATAGCGACCAGAGCGGGAGAAACCGCAGGCAGATGTCGTTGCTTTTTCCGTGGGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTgcggcg                                                                                                                                            <  2:491054/280‑1 (MQ=255)                                                                                                                                                 tg‑tgGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGt                                                                                                                                           >  2:138791/1‑280 (MQ=255)                                                                                                                                                   gttggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTGTTGGTGATAGTCCGTTGCCGGGCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACAGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCGGAGTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTa                                                                                                                                          <  1:220972/277‑1 (MQ=255)                                                                                                                                                    ttggCCCACGCCCTCCCGGCTTGCGGCTTTCCATCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACa                                                                                                                                        <  2:307443/279‑1 (MQ=255)                                                                                                                                                    tgGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTa                                                                                                                                          <  1:554314/279‑1 (MQ=255)                                                                                                                                                     gggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCCGATTTCGTTGATTTTTCCGTGAGGGGCTCTTTTTTC‑GCCGCGACTGTGCCGGTTGGCTGCGGGGTACAt                                                                                                                                       >  1:720335/1‑281 (MQ=255)                                                                                                                                                     gggCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACAt                                                                                                                                       >  1:669314/1‑281 (MQ=255)                                                                                                                                                       gcgcACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAACGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCTCTTTCTGGCTTTGCAGCGCCTCGCACTCTTCTCTCTTGTTATGCGTACTGATTTACAGATAACCGATAACGGTCACAAGAACGGCGGCCGTAATAGCGACTATAGCCGGAGAAACCGCAGGCAGCTTTCGTTGCTTTTTCCCTGTGGTGC‑CTTCTTTCAGCCGCCATGGTGCCCGCTGGGTGGCGCGTatccaa                                                                                                                                     >  2:223998/1‑276 (MQ=255)                                                                                                                                                         gcacgcacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGCTGGCTGCGGCGTACATAAt                                                                                                                                    >  1:918218/1‑280 (MQ=255)                                                                                                                                                         gcacacacTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCACCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAAt                                                                                                                                    >  1:795057/1‑280 (MQ=255)                                                                                                                                                          cacgcacTCCGCTCTGGCGACTTGCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTTGTAGTCCGTTTCCTGTCACTGTCTGGCTTTGCAGCGTCTCTGTCTCTTCTTTCTTGTGATGCGTAATGAAGTACCGACCACCGATAAAGGTCACAGGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAAtct                                                                                                                                  <  2:667275/281‑1 (MQ=255)                                                                                                                                                                cTCCCGGCTTGCGCTTTTCCAGCCCTTTATTGT‑AGGTCCGGTGTTCTTCTGGTTTTTGTGTTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGATTCTTCTTTCTTGTGATGCATAATAAAGTACAGACCACCGATAAAGGTCACAAGAACAGCGGCAGCAATAGCGACCATAGCGGGAGAGGCCGCAGGCAGATTTCGTTGCTTTTTCCGTAAGGTGCTCGTTTTTC‑GCAGCGAAGGTGCCGGTTGACTGCGGCGTACATAATCTCGTtg                                                                                                                             <  2:640336/280‑1 (MQ=255)                                                                                                                                                                cTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTtg                                                                                                                             <  1:214678/280‑1 (MQ=255)                                                                                                                                                                   ccGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGTGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCCGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTCGGCTGCGGCCTACATacttctgttttgcc                                                                                                                         >  1:505972/1‑268 (MQ=255)                                                                                                                                                                         ggCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTg                                                                                                                                                 <  1:727379/251‑1 (MQ=255)                                                                                                                                                                         ggCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTg                                                                                                                                                 >  2:727379/1‑251 (MQ=255)                                                                                                                                                                         ggCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATACCGGGAGAAACCGCAGGCAGATTTCGTTTCTTTTTCCGTGGGGTGCTCTTTTTTC‑GCCGCCAAGGTTCCGGTTGGCTGGGGCTGTCCTTATCTCGTTGTGCCACTTTc                                                                                                                   >  2:549449/1‑281 (MQ=255)                                                                                                                                                                             aCTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCCGCGTCTCGGACTCTTCTTGCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTGAGTTGCTTCTTCCGTGCGCTGCTCTTTTTTA‑GCGGCGCAGGTGCCGGTTGGCTGCGGCGTACATAATCTCTTGGTGCCCCTATCGttt                                                                                                               >  2:774565/1‑281 (MQ=255)                                                                                                                                                                             aCTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGTGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGttt                                                                                                               >  1:1035670/1‑281 (MQ=255)                                                                                                                                                                                ttCCATCTCTTTAATTT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCg                                                                                                             <  1:371393/280‑1 (MQ=255)                                                                                                                                                                                ttCCAGCTCTTTAATGT‑ATCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCg                                                                                                             <  1:1009329/280‑1 (MQ=255)                                                                                                                                                                                ttCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCg                                                                                                             <  1:549449/280‑1 (MQ=255)                                                                                                                                                                                 tCCGGCTCTTTCATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCt                                                                                                           <  1:718672/281‑1 (MQ=255)                                                                                                                                                                                 tCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACGATCGTTTCGCt                                                                                                           >  1:986754/1‑281 (MQ=255)                                                                                                                                                                                           tAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTAttc                                                                                                 >  1:563659/1‑281 (MQ=255)                                                                                                                                                                                                    cgcCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCa                                                                                         >  1:249578/1‑281 (MQ=255)                                                                                                                                                                                                    cgcCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCGGTATTTATTCGTTCGTCa                                                                                         >  2:1039728/1‑281 (MQ=255)                                                                                                                                                                                                        aGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATATCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTTAGccc                                                                                     >  1:921453/1‑281 (MQ=255)                                                                                                                                                                                                              cttctGTTTTTGGTGTTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGGGGTGCTCTTTTGTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCAt                                                                                <  1:1056495/280‑1 (MQ=255)                                                                                                                                                                                                                     ttttGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTa                                                                        <  1:138791/281‑1 (MQ=255)                                                                                                                                                                                                                         ggtggtAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGTCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGGCGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTGGCGGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAgcg                                                                    >  1:739029/1‑281 (MQ=255)                                                                                                                                                                                                                         ggtggtAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCCCTTTTTTC‑GCCGCGAGGGTGCCGGTTGGCTGCGGCGTACATCATCTCGTTGTGCCACTATTGGTTCTCGGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAgcg                                                                    >  2:826108/1‑281 (MQ=255)                                                                                                                                                                                                                             gtAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGCTGCGTAATTAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAgcggcg                                                                 <  1:774565/280‑1 (MQ=255)                                                                                                                                                                                                                               aGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAACGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGc                                                              >  1:420745/1‑281 (MQ=255)                                                                                                                                                                                                                                     tttCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGTGGCGGGCCTTTGa                                                        >  1:945445/1‑281 (MQ=255)                                                                                                                                                                                                                                     tttCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTCCCGGTTGGCTGCGGCGTACATCATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCCGCATGTTACTTAAGCGGCGGTCCTTTg                                                         >  2:327263/1‑280 (MQ=255)                                                                                                                                                                                                                                      ttCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGTGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGa                                                        <  2:1035670/280‑1 (MQ=255)                                                                                                                                                                                                                                      ttCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGa                                                        <  2:669314/280‑1 (MQ=255)                                                                                                                                                                                                                                             cACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGc                                                                                                                          >  2:155491/1‑207 (MQ=255)                                                                                                                                                                                                                                             cACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGc                                                                                                                          <  1:155491/207‑1 (MQ=255)                                                                                                                                                                                                                                                tttggtgttttGAAGCCGCTCTGCCTCTTCTTTGTTGTGATGCGTAATGAAGTCACGACAACCGATAAAGGTCACAAGGACGGCGGCAGCAATATCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTGTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGc                                             <  2:720335/273‑1 (MQ=255)                                                                                                                                                                                                                                                tttCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTTATGCATACTGAAGTACAGACCACCGATAAAGGTCACAGGACCGGCGGCAGCAATAGCGACCATAGCGGGAGAACCCGCAGGCAGATTTCGTTGCTTTTTCCCTGAGGTACTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGc                                             <  1:167169/281‑1 (MQ=255)                                                                                                                                                                                                                                                 tccTGGCTTTACGCCGTATCAGACTGTTCTTTGTTGTGATGCGTGGTGAACTACACACCCCCGAAAAGGGTCACAAGAGCGGCGGCAACAATTGGGACCATGGCGGGAGAAACCGACGGCAGATTTCGTGGCTTTTTCCGTGGGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGTCCAGTCACGGCa                                            <  2:326681/279‑1 (MQ=255)                                                                                                                                                                                                                                                       cTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTa                                      <  1:1051714/281‑1 (MQ=255)                                                                                                                                                                                                                                                          tGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GTCGCGAAGGCGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGGATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCAGGGCAGTCCTAAAg                                   >  1:404420/1‑281 (MQ=255)                                                                                                                                                                                                                                                            cAGCGTGTCGGTCTCTTCTTTCTTGTGATCCTTAATGAAGTACAGACAACCGATACAGGGCACAAGTACGGCGGCATCAATAGCGACCATCGCGGGAGAAACCGCAGCCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCGACTATCGTTTCGCTGTAGTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGt                                 <  2:739029/281‑1 (MQ=255)                                                                                                                                                                                                                                                             aGCGTCTCGGACTCTTCTTTATTGTGGTGCGTAATGAAGTGCAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGtt                                <  1:223998/281‑1 (MQ=255)                                                                                                                                                                                                                                                             aGCGTCTCCGACTCTTCTTTCTTGTGATGCGTAATGAAGTCCATACCACCCATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGtt                                <  2:986754/281‑1 (MQ=255)                                                                                                                                                                                                                                                              agctctcGGACTCTTCTTTCTTGGGATGCGTAATGAACGACAGACCACCAAAAAAGGTAACAAGAACGACGGCAGCAATAGCGACCATGGCGGGGGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGGGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGtt                                <  2:483137/277‑1 (MQ=255)                                                                                                                                                                                                                                                                   tcGGCCCCTTCTTTCTGTGGTTGCTTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACttt                          <  2:360071/281‑1 (MQ=255)                                                                                                                                                                                                                                                                        attttctttctGGTGGTGGGTAATGGAGTAACAACCGCCGATCAAGGTCACAAGAACGGCCGCAGCAAAAGCGACCATAACGGGAGAAACCGCAGGATGATTTGGTTTGTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGGGTACAAAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGt                     <  2:945445/278‑1 (MQ=255)                                                                                                                                                                                                                                                                                tcttGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCGCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGTAGTCTTAAAGGTTTACCTTCAGGTAAcgcgc              >  1:446003/1‑280 (MQ=255)                                                                                                                                                                                                                                                                                   tgtgATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCCCGTTGTGCCACTATCGTGTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCgtgt          >  1:487192/1‑281 (MQ=255)                                                                                                                                                                                                                                                                                     tgATGTGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGa                                                        >  2:143941/1‑233 (MQ=255)                                                                                                                                                                                                                                                                                     tgATGTGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGa                                                        <  1:143941/233‑1 (MQ=255)                                                                                                                                                                                                                                                                                     tgATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGtt                                <  1:821016/257‑1 (MQ=255)                                                                                                                                                                                                                                                                                     tgATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGtt                                >  2:821016/1‑257 (MQ=255)                                                                                                                                                                                                                                                                                          cGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGGGAAGGTGCCGGTTGGCTGGGGCGTACATAATCTCGTTGTGCCCCTATCGGTTCGCTGTATTTATTCGTTCGTCAGCCCCCACTGTTACTTAAGCGGCGGGCCTTTGACCAGGCACGGCAGTCTTAAAGGGTTAATTTAAGGGAAcgggggggtttgaccc   >  2:564214/1‑267 (MQ=255)                                                                                                                                                                                                                                                                                          cGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTCTTTATTCGGTCGTCAGCCCGCCATGTTCCTTAAGCGTCTGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTCACTTTAATGTAACGTGCGTGTTG‑Tcccc  >  1:627226/1‑281 (MQ=255)                                                                                                                                                                                                                                                                                          cGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATccc   <  1:1039728/281‑1 (MQ=255)                                                                                                                                                                                                                                                                                          cGTAAGGAAGTCCCGACCCCCGATAAAGTTCACAAGAACGGCGGCAGCAATCGCGACCGTAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATccc   <  2:563659/281‑1 (MQ=255)                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                           CTGTTCCGGCGTCTGTTCATTCCACGGAACTTCAACCAGCTGCGTTGGCTGCTGGCGCATATCAGCCTGCATTTGTTCAAGAAGCTGACGTTGTTCTGGTGTCAGTTGCTCCGGCGTTTTCACTTCACCACCGGCAGAAGGTTCTG‑TGGGCGCACGCACTCCCGGCTGGCGACTTTCCAGCTCTTTAATGT‑AGCGCCAGCGTTCTTCTGGTTTTGGTGGTAGTCCGTTTCCGGTCACTTTCTGGCTTTGCAGCGTCTCGGACTCTTCTTTCTTGTGATGCGTAATGAAGTACAGACCACCGATAAAGGTCACAAGAACGGCGGCAGCAATAGCGACCATAGCGGGAGAAACCGCAGGCAGATTTCGTTGCTTTTTCCGTGAGGTGCTCTTTTTTC‑GCCGCGAAGGTGCCGGTTGGCTGCGGCGTACATAATCTCGTTGTGCCACTATCGTTTCGCTGTATTTATTCGTTCGTCAGCCCGCCATGTTACTTAAGCGGCGGGCCTTTGACCAGTCACGGCAGTCTTAAAGGTTTACTTTAAGGTAACGCGCGTGTTGATCCCC  >  NC_000913/4122896‑4123456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑