Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3610C.92.9% 370.4 / 18.7 98pseudogene (2/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base . (55/36);  total (58/40)
Fisher's exact test for biased strand distribution p-value = 4.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.
*NC_0009132,173,3620C.92.0% 355.6 / 13.2 98pseudogene (1/442 nt)gatCpseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base . (54/36);  total (58/40)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

TACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGC  >  NC_000913/2173100‑2173634
                                                                                                                                                                                                                                                                      ||                                                                                                                                                                                                                                                                                 
tACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCagcagc                                                                                                                                                                                                                                                               <  1:46892/281‑1 (MQ=255)
  cacccTGCTTTTAAGGCCCCCGGATTGGCCGCCAGTTGGGGGTGCAAACGCTTAGTTTGCGTGGGGATCCACATGGTGTTGCTCATAATGATGACGCCCGAGATTAAGGTGGGGACCACATTTCCACGGTGCAGCGCGACCGCCACAGCCACGAAGAAGTCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGAACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCGGATACCA‑‑CCGCCGTATGTCCCagcagc                                                                                                                                                                                                                                                               <  2:1150127/277‑1 (MQ=255)
       ggCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACCCAAACA‑GCAATTAAAATGGTGAGTGGGCTAAAAATCAGGCTTGCCGATCCCA‑‑CCTCCGTTGGTCCCAGCAGCACCGcc                                                                                                                                                                                                                                                         >  1:666596/1‑280 (MQ=255)
       ggCTTTTAACGCCCCAGCAGTAGCCGCCAGTTGGGTGTGCAAACCAATAGCTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGGGGCAAGCTCGCCAAACGGCAGCCCCCGAGTCACCGGCACCCAAACA‑GCACCTAAAAGGGTGAGTGGGCTAAAAATCAGGGTTGCAGATACCA‑‑CCGCCGTATCTCCAAGCAGCAACGcgg                                                                                                                                                                                                                                                        >  2:692737/1‑279 (MQ=255)
        gCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATCATGATGACATCCTAGATTAAGGTGCGGAACAGATTTCCCCGACGCACGGCGACCGCCATCGCCCAGACGAAGCCGATGGTGGCAAGCTCGCCAAACGGCAACACCTTATTTCCCGGCACACACACACGCAA‑CAAAATTGTGCGGGGGAAAAAAATCAGCCTTGTCGAT‑‑CACAACCCCGTATGGCCcagccacacatcct                                                                                                                                                                                                                                                        >  2:1093077/1‑270 (MQ=255)
           ttgaaCGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAAACAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATc                                                                                                                                                                                                                                                    <  1:602599/278‑1 (MQ=255)
                       tccggaGCCTCCAGGTGGGTGTGCAAAACAAGAGGTTGGGTAGCGATCCGCCGGGTGATGCTCATAATGATGACAACGGAGCTTAAGGTGCGGAACAGTGTTCCGAGGTGCACGGGCACCGCCATCGCCACGCAGAAGCCGATCGTGGCAGGATCGCCAAACGGCAGTAGCTGATTACCCGAGACACAAACA‑GCAATTAAAATGGTGAGTGGGAGAAATATCAGGCTTGCAGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAAGCAg                                                                                                                                                                                                                                        <  2:1198646/276‑1 (MQ=255)
                                 ccAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATTATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATCGTGGCAAGATCGCCAACCGGCAGCACCTGATTACCCTGCACACAAACA‑GTCATTAAAATGGTGAGCGGGATACAACTCAGGCTTGCCGATACCA‑‑CCGCCGATTCTCCCAGCAGCAACGCCGGATCACGGCACACCAGGAAccccct                                                                                                                                                                                                                               >  2:110000/1‑278 (MQ=255)
                                  cAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTg                                                                                                                                                                                                                               >  1:1058542/1‑279 (MQ=255)
                                                       aGTTTGGGTCGCGATCCACAGGCTGATTCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTaaa                                                                                                                                                                                                        <  1:576477/280‑1 (MQ=255)
                                                           tGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATA‑‑ACACCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACCCCTGACCGCCGAACTTC‑GCCGGTAAACg                                                                                                                                                                                                      >  1:1172416/1‑278 (MQ=255)
                                                             cGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTa                                                                                                                                                                                                  >  2:808771/1‑280 (MQ=255)
                                                                 gcgATCCCCAGGGTGATGATCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAAAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGc                                                                                                                                                                                             <  1:196615/281‑1 (MQ=255)
                                                                  cgATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGACCTTC‑GCCTGTAAACGACTAAGAGcc                                                                                                                                                                                            >  1:817347/1‑281 (MQ=255)
                                                                          aGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                                                                                                                                                               <  1:887583/223‑1 (MQ=255)
                                                                          aGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                                                                                                                                                               >  2:887583/1‑223 (MQ=255)
                                                                           gggTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCg                                                                                                                                                                                   >  1:1030264/1‑281 (MQ=255)
                                                                            ggTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAACAAGGCCACTCAGGAACTCCTGACCGCCGAACTGC‑GCCTGTAAAAGACTACGAGCCTGCTTAGcaa                                                                                                                                                                                  >  2:397013/1‑279 (MQ=255)
                                                                            ggTGATGCTCATAATCATGACATCCGAGACTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCTGACGGTGGCAAGATCGCCAAACGGCAGCACCTGCTTACCCGGCACGCAAACA‑GCAATTAAAATGGTGAGAGGGATAAAAATAAGGCTTGCCGAT‑CCA‑ACCGCCGTAGGTACCAGCAGCAACGTCGGATCAAGGACAATCAGTAACCCCAGACCACCGAAATTC‑CACTGGAAAAGACGACGAGACTGGGTAGcaa                                                                                                                                                                                  >  2:878249/1‑279 (MQ=255)
                                                                             gTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                                                                 <  1:808771/281‑1 (MQ=255)
                                                                               gATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCCGCTTAGccagg                                                                                                                                                                                >  2:1168379/1‑276 (MQ=255)
                                                                                aTGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTAACCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGACAAAAATCAGGCTTGCCGATACCA‑‑CAGCCGTAAGTCCCAGCAGCAACGCCGCATCAAGGCCAATCCGGAACACCTGACCGCCGGACTCC‑CCCCGTAAACGACTGCGACGCTCCTTAGCGGTgg                                                                                                                                                                               >  2:322027/1‑280 (MQ=255)
                                                                                aTGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTACCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTTCCAGCAGCAAAGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGCACTTC‑GCCTGTAAACGACCACGAGCCTGCTTAGCGATgt                                                                                                                                                                               >  1:1015179/1‑279 (MQ=255)
                                                                                 tGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGc                                                                                                                                                                             >  1:817905/1‑281 (MQ=255)
                                                                                    tCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGACCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGCTGGGCGt                                                                                                                                                                           >  1:879333/1‑280 (MQ=255)
                                                                                     cATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCg                                                                                                                                                                            <  1:1080510/278‑1 (MQ=255)
                                                                                      aTAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGCTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGAATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACACCTGACCGCCGAACTTC‑GCCTGTAACCGACTACGAGCCTGCTTAGCGATGGGCGTTaa                                                                                                                                                                        >  1:73747/1‑281 (MQ=255)
                                                                                        aaTGATGCCAGCCGAAGTTCAGGTGCGGAACCGCGTTCCACACTCCACGGCGACCGCCATCGCCACGAAGGAGCCCATCGTGGCAAAATCGCCAAACCGCAGCACCTGATTACCCGGCACACAAACA‑GCAATGAAAATGGTGAGTGTGATAAAAAGCATGCTTGCCCAGACCA‑‑CCGCCGTATGTCCCAGCAGCCACGCCGGTTCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                                                                                                                                                                      <  2:817347/281‑1 (MQ=255)
                                                                                        aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑Gc                                                                                                                                                                                                               <  2:499755/240‑1 (MQ=255)
                                                                                        aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑Gc                                                                                                                                                                                                               >  1:499755/1‑240 (MQ=255)
                                                                                        aaTGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTAAGAGCCTTCTTAGCGATGGGCGTTAAAc                                                                                                                                                                      >  1:258888/1‑281 (MQ=255)
                                                                                         atgatgACACCCGTGTTTAAGGTGCGGACCAGATTTCCACGATGCACGGCGACCGCCATCGCCCCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                                                                                                                                                                      <  1:335710/280‑1 (MQ=255)
                                                                                           gatgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCt                                                                                                                                                                                                             >  1:349070/1‑239 (MQ=255)
                                                                                           gatgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCt                                                                                                                                                                                                             <  2:349070/239‑1 (MQ=255)
                                                                                            atgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATCAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGGCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑TCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatc                                                                                                                                                                  >  1:393745/1‑281 (MQ=255)
                                                                                             tgaCACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatcc                                                                                                                                                                 >  1:185569/1‑281 (MQ=255)
                                                                                                cacCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatg                                                                                                                                                              <  1:596446/281‑1 (MQ=255)
                                                                                                     gacatagaGGTTCGGGACAGATTTCCAAAATGCCGGGCGACCTCCATCGCCACGGAACAGACGATTGTAGCAATTGAGCCAAACGGCAGCACCTGATTACCCAGCACACAAACA‑CCAATTAAAATCGTTGGTGTGAGAAATTTCAGGCTTGCCCATAC‑‑CGCCGCCGTATGTCCCAGCAGCAACGCCGGATCAGTGCCAATCAGGAGCGCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGTGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATggg                                                                                                                                                         <  2:73747/274‑1 (MQ=255)
                                                                                                     agggacaggttGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGACGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAGCTTC‑GCATGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATgg                                                                                                                                                          <  1:22584/270‑1 (MQ=255)
                                                                                                      agaTTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGACAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGACGTATGTACCAGCAGGAACGCAGGAACAAGGCAAAACAGGAACTCCAGACCGCCGAAATTG‑GACTGTAAAAGACCACGAGCCCGCCGTGCGAAGGGCGTTAAACCATCAATGATtgg                                                                                                                                                         >  2:1113399/1‑280 (MQ=255)
                                                                                                             ggTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCt                                                                                                                                                                                           >  1:226002/1‑239 (MQ=255)
                                                                                                                 cGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAccc                                                                                                                                              <  1:1168379/280‑1 (MQ=255)
                                                                                                                   gAACAGATATCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGTTCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATCAAAATGGTGAGTGGGATTAAACTCAGGCTTCCCGATATCA‑‑CCACCGTATGTCCCACCAGCAACGCAGGATGAAGGCTACTACGGAACTCCGTACCGCCGAACTTC‑CACTGTAAACCACCCCGAGCCTGCCGAGCGATGGGTGCTAACACATCCATGCTGCTTCCAATCAACCGTg                                                                                                                                           >  2:569716/1‑281 (MQ=255)
                                                                                                                      cAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGCTGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCa                                                                                                                                        >  1:699150/1‑281 (MQ=255)
                                                                                                                        gATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATTGCCAAACGGCATCACCTGATTACCCGGCCCACAAACA‑GCAAATAAAATGTCGCGTGGGATATAAATCAGGCTTGCCGATACCA‑‑CCGCCGGAGGTTCCAGCACCAACGCCGGCACAAGGCCAACCAGGAACTCCTGACCGCCGCAGTTT‑GCCTGTAACAGACTACGACACCGCTTACCGATGGGGTTTATACCATCCATGTGGGTTTTAATCccccccactct                                                                                                                                       >  2:22584/1‑273 (MQ=255)
                                                                                                                         aTTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCAtt                                                                                                                                      >  1:359193/1‑280 (MQ=255)
                                                                                                                           ttCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTagc                                                                                                                                   >  1:252664/1‑281 (MQ=255)
                                                                                                                              cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCC‑‑CCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCCTTAAACCATCCATGATGGGTTTAATCACCCGTAGCATTagcag                                                                                                                                 >  1:793535/1‑280 (MQ=255)
                                                                                                                              cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                                                                                                                                                                   >  1:482936/1‑246 (MQ=255)
                                                                                                                              cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                                                                                                                                                                   <  2:482936/246‑1 (MQ=255)
                                                                                                                              cACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCAAATCAGGAAATCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACAATCCATGATGGGTTTTATTACCCGCGGAATTagacg                                                                                                                                 >  1:674990/1‑277 (MQ=255)
                                                                                                                                        ggCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACT‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CTGCCGTATGTCCCAGCAGCAACGCCGGATCAACGCCAATCAGGAACTCCTGACCGCCGAACTTC‑TCCGGTAAACGACTACGAGCCTGCTTAGCGATGGGCGCTAAACCATTCATGATGGGTTTAATCACCCGTGGCCTTAGCAGCATTACTgca                                                                                                                       >  1:1121680/1‑279 (MQ=255)
                                                                                                                                        ggCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGTCTGCTTAGCGATGGGCGGTAAACCATCCATGATGGGTTTAATCACCCGTGGCATCTGCAGCATCTCTgccg                                                                                                                      >  1:673756/1‑281 (MQ=255)
                                                                                                                                             ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATTACTgccgcctt                                                                                                                  >  1:1069605/1‑278 (MQ=255)
                                                                                                                                                    cGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAccc                                                                                                                                              >  2:1147165/1‑245 (MQ=255)
                                                                                                                                                    cGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAccc                                                                                                                                              <  1:1147165/245‑1 (MQ=255)
                                                                                                                                                      cccccaagaagCCGATGGTGCGACGAACGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGTTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                                        <  2:1058542/276‑1 (MQ=255)
                                                                                                                                                        aCGAAGGAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAg                                                                                                      <  1:1224738/281‑1 (MQ=255)
                                                                                                                                                        aCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgcc                                                                                                                                                                                                                                                                                >  1:852245/1‑111 (MQ=255)
                                                                                                                                                        aCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATaccaccgcc                                                                                                                                                                                                                                                                                <  2:852245/114‑4 (MQ=255)
                                                                                                                                                        aCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAg                                                                                                      <  1:559453/281‑1 (MQ=255)
                                                                                                                                                        aCGAAGAAGCCGATGGGGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAg                                                                                                      <  1:397013/281‑1 (MQ=255)
                                                                                                                                                         ggaagaaGCCGAGGGTGGCAAGTTAGCCAAACGGCACCACCTGATTACCCGGCACACAAACA‑GCGATAAAAATGGTGACTGGGATAAAATTCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCTACGCCAGTTCAAGGCCACTCAGGAACTCCTTACCGCCGAACTGC‑GCCTTTAAACTACTACTAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAg                                                                                                      <  2:252664/279‑1 (MQ=255)
                                                                                                                                                                gCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATa                                                                                              <  2:359193/281‑1 (MQ=255)
                                                                                                                                                                    aTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGGTTTTACCGCCAGCTGCAATacac                                                                                           >  1:169832/1‑280 (MQ=255)
                                                                                                                                                                          gaaaGAGCGCCAAACGGCAGCACCTGATTACCCGGGACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATAAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTg                                                                                      <  1:110000/277‑1 (MQ=255)
                                                                                                                                                                               aTCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCt                                                                                                                                                                                                                                >  2:568692/1‑137 (MQ=255)
                                                                                                                                                                               aTCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCt                                                                                                                                                                                                                                <  1:568692/137‑1 (MQ=255)
                                                                                                                                                                                 cGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCCGCGCCAATACACCTTTGGAATCGTaa                                                                             >  1:878551/1‑281 (MQ=255)
                                                                                                                                                                                        cGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCcgcg                                                                       <  1:692737/280‑1 (MQ=255)
                                                                                                                                                                                           cagacccTGATTACCCGGCACAGAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGa                                                                   <  1:1113399/276‑1 (MQ=255)
                                                                                                                                                                                              caCCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGc                                                                >  1:457978/1‑281 (MQ=255)
                                                                                                                                                                                              caCCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGAATTAGCAGCACCACTGCCGCCGTTTTTACCGCCCGCTGCAATAAACCTTTGACATCGTAACCCCCGAGGATGc                                                                >  2:37845/1‑281 (MQ=255)
                                                                                                                                                                                               aCCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGcc                                                               <  1:322027/281‑1 (MQ=255)
                                                                                                                                                                                                         ccGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTTCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTATCGATGGGCGTTAAACCATCCATTATGGGTTTAATCACCCGTGGCATTCGCAGCATAACTGCCGCCGCTTTTACCGCCAGCTGAAATACACCTTCGACATCCAAACCCGCCAGGCTGCCGATCATCAGc                                                     >  2:825859/1‑281 (MQ=255)
                                                                                                                                                                                                           ggCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGccc                                                   >  2:43704/1‑281 (MQ=255)
                                                                                                                                                                                                              acacaAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAt                                                                                                                                                   >  1:73402/1‑182 (MQ=255)
                                                                                                                                                                                                              acacaAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAt                                                                                                                                                   <  2:73402/182‑1 (MQ=255)
                                                                                                                                                                                                                  aaaCA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTTGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcaca                                            >  1:8595/1‑281 (MQ=255)
                                                                                                                                                                                                                  aaaCA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATcaca                                            >  1:1222497/1‑281 (MQ=255)
                                                                                                                                                                                                                      a‑GCAATTAAAATGTTGAGTGGGATAACAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCACTCAGGCCCTCCTTGCCGCCGAACTTC‑GCCTGTCAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAAc                                        <  2:1172416/281‑1 (MQ=255)
                                                                                                                                                                                                                          aaTTAAAATTGTGAGTGGGATATAAATCAGGCTTGCCGTGACCA‑‑CCGCCGAATGTCCCAGCAGCAATGCCGTATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCa                                     <  1:825859/281‑1 (MQ=255)
                                                                                                                                                                                                                          aaTTAAAATGGTGAGGGGGATAAAAATCAGGCTTGCCGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGTCCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCGTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCa                                     <  1:1093077/281‑1 (MQ=255)
                                                                                                                                                                                                                           aTTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCAc                                    >  2:131081/1‑281 (MQ=255)
                                                                                                                                                                                                                            ttAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                                        <  2:280397/212‑1 (MQ=255)
                                                                                                                                                                                                                            ttAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                                        >  1:280397/1‑212 (MQ=255)
                                                                                                                                                                                                                                  tGGTGAGGGGGATAAAAGTCAGGCTTGCCGATACCA‑‑CCGACGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                                                                                                                                                                 <  2:37667/133‑1 (MQ=255)
                                                                                                                                                                                                                                    gTGAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATgg                                                                                                                                                                               >  1:37667/1‑133 (MQ=255)
                                                                                                                                                                                                                                      gAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGTGCGTTAAACCATCCATAATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATAAACCTTTGACATCGTAACCCGCTAGGATGACGCTAATAAGCCCCCTCACAAAATCCACGGTGACACGCt                         >  1:363825/1‑281 (MQ=255)
                                                                                                                                                                                                                                      gAGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGAACAAGGCCAATCAGGAACTCCTGATCGCCGAATTTC‑GCCTGTAAACGACTACGATACTGCTTAGAGATGGGCGCTAAACCATCCATGCTGGGACTAATCACCCGTGGCATTAGCAGCACCTATGCCGCCGTTCTTTTCGCCAGCTGCAATCCTTCTACGACACCGTACCCCGCGCGGCTGCCATTATTCAGCCCCATCACACAACCCACGGTGACCGGCt                         >  2:602869/1‑281 (MQ=255)
                                                                                                                                                                                                                                       aGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCg                                                              <  2:43975/243‑1 (MQ=255)
                                                                                                                                                                                                                                       aGTGGGATAAAAATCAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCg                                                              >  1:43975/1‑243 (MQ=255)
                                                                                                                                                                                                                                                      aGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                                        <  2:432816/186‑1 (MQ=255)
                                                                                                                                                                                                                                                      aGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGcc                                                                                                        >  1:432816/1‑186 (MQ=255)
                                                                                                                                                                                                                                                      aGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCAACGTTGCCAGGCTCGCCAAATGGACCaaa         >  1:498584/1‑281 (MQ=255)
                                                                                                                                                                                                                                                      aGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACACGCGAGGATGACGATAATCAGCCTCATCACAAAACCCACGGTGACAGGCTCGCAAAATGGAcc            >  1:798466/1‑278 (MQ=255)
                                                                                                                                                                                                                                                            gCCGGTACC‑CCCCGCC‑TAGTTCCCACAACCAACTCCGATTCAAGGCCAATCAGGAATCCCTAAACGCCGAACTTCTGCGTG‑AAAAGCGTAGCGGCGTGGTTAGCGATGGGCGTTAAACCAGCCATGCTGGGTTTAAGCACCCGTGGCATTAGCAGCATCCCTGCCGCCGTGTTTACCGCCAGCTGCAATACACCTTAGGCATCGTAACCCGCGAAGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTGgcg   <  2:699150/281‑1 (MQ=255)
                                                                                                                                                                                                                                                            gCCGATACCA‑‑CCGCCGTATGTTCCAGCAGCAACGCCGGAGCAAGGCCAATCAGGAACTCCTGACTGCCGAACTTC‑GCCTGTAAACGACTACGAGGCTGCTTAGCGATGGGCGATAAAGCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTGTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCTCCATCACGAAACCCACGGTGACAGGCGCGCAAAATGGACCAAAtcggc    >  1:732955/1‑276 (MQ=255)
                                                                                                                                                                                                                                                            gCCGATAC‑‑CCCCGCCGTATGTCCCTGCAGCAACGCCGAATCAAGGCCAATCAGGATCTCCTGACCGCCGAACTTC‑GCCTGTCAACGACTCCGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTgcg   <  1:878249/281‑1 (MQ=255)
                                                                                                                                                                                                                                                             ccGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTgcgc  >  1:458621/1‑281 (MQ=255)
                                                                                                                                                                                                                                                                      ||                                                                                                                                                                                                                                                                                 
TACCCCCGGCTTTTAACGCCCCAGCATTAGCCGCCAGTTGGGTGTGCAAACCAATAGTTTGCGTCGCGATCCACAGGGTGATGCTCATAATGATGACACCCGAGATTAAGGTGCGGAACAGATTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACA‑GCAATTAAAATGGTGAGTGGGATAAAAATCAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTC‑GCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCCGTGGCATTAGCAGCATCACTGCCGCCGTTTTTACCGCCAGCTGCAATACACCTTTGACATCGTAACCCGCGAGGATGCCGATAATCAGCCCCATCACAAAACCCACGGTGACAGGCTCGCCAAATGGACCAAATTTGCGC  >  NC_000913/2173100‑2173634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑