Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,560,4551.G98.4% 206.8 / NA 64intergenic (‑2/+1)glpR/glpRpseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (1/0);  major base G (25/36);  minor base A (0/1);  total (26/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGC‑‑TGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATC  >  NC_000913/3560182‑3560715
                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                    
ccACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGC‑‑TGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGc                                                                                                                                                                                                                                                                       <  2:726285/274‑1 (MQ=255)
 cACCGGCGAGAATGACGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATCGC‑‑TGTGATTGAGCAGTGCGTGCGCTACCGCCTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGTGTGGCCTTGCGATCGTGCCACGGCGTGTGAACCGAACTGGAGGGCAGCGCCGCACCGCCATGATGGCCa                                                                                                                                                                                                                                                                    >  2:127123/1‑275 (MQ=255)
      gCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGC‑‑TGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCGCAGCCGCACCGCCATGATGGcccagga                                                                                                                                                                                                                                                                >  2:70761/1‑269 (MQ=255)
      gCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGC‑‑TGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGa                                                                                                                                                                                                                                                                 >  1:343236/1‑274 (MQ=255)
                     aaaTCTTCTTTTACGTTCGACGGGTTCGCAACGTTGAGATTGTATGGGACAATGCTCAAATTGC‑‑TGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGCTATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCTCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGTTCCg                                                                                                                                                                                                                                                  <  1:711624/274‑1 (MQ=255)
                                 aCCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGCTGTGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                                                                                                                                                        <  1:18297/274‑1 (MQ=255)
                                    ctCAAAGTGTTAGCAACGTTGGGATTGTTGGTGACAATGCGCAAATTGC‑‑GTTTATTGAGCATTGCGGGCGCTACCCCTTCCGGCTGGGTGCCGATTTCGATAAGCAGCGTCGAGCCATGGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTc                                                                                                                                                                                                                                  <  2:110597/274‑1 (MQ=255)
                                      caaggggtTGGCAGCGTTTGGGTTGTTGGTGACAATGCGCAAATTGC‑‑TGTGATTTGGCGGTGCGTTGGTTACCGCTTCCGGCGTGGGGCCGGTATCTATAAACAGCGTCGGGCCCTGGGGGATTGGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTcgc                                                                                                                                                                                                                                <  2:903167/269‑1 (MQ=255)
                                      cAACGTTGTTGCAACGTTCAGAGTGTGTGTGACAATGCGCATAGTGC‑‑TGTGATAAGGAAGTGCGTGCGCTACCGCTTCCGGCGGGGGGCCGATATCGATAAACAGCGTCGGGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCGCTTTTGCTTCGGTCTGGGGGGGCTGGCGATCGTGCCACGGCGTGTTAGCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGATTTGCTCCGCCAGCTCATTGAGGTcgc                                                                                                                                                                                                                                <  2:394161/275‑1 (MQ=255)
                                      cAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGC‑‑TGTGATTGGGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTcgc                                                                                                                                                                                                                                <  1:127123/275‑1 (MQ=255)
                                             tgtgCAGCGTTGGGTTTGCTGTTGACAATGCGCAAATTC‑‑TGTTCATTGGCCAGTGCGTGCGCTACCGCTTCGGGGGTGGTGCGGATATCGCTGAGCGGCGTCGGGCCATTGGGAATTTGCTCCGCCACTTTGCGGGGGATGCGCTCTTTTTCTTCGGTCGGGGTTGCCTTGCGATCGTGCCGCGGCGTTTTAACCGAACTATAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGGAt                                                                                                                                                                                                                         <  2:226317/272‑1 (MQ=255)
                                               aGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGC‑‑TGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCAGTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCAGGATGGCGCAGGATCAGGTGTGTCGCCGCCAGGTCACTGAGGTCGCGGAGAATAg                                                                                                                                                                                                                       >  1:688504/1‑275 (MQ=255)
                                               aGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGC‑‑TGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCAATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGCTGGCCTTGCGATCGTGCCACGGCGTGTTAAACGCACCGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGACCAGGTTTTGCCCCGCAAGCTCATTGAGGTACCGGCGAATAg                                                                                                                                                                                                                       >  1:456780/1‑275 (MQ=255)
                                                       tGAGATTTTTAGAGGCGATGCGCAATTGGC‑‑TGCGATTGGGCGAGTCGAGAGCTACCGCTTCCAGCGTTGTGCCGATATCTGTAAACAGCGTCAAGCCATTGCGGATTTGCTCCGCCACTTTGCGGGCTATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACAGCGTGTTAACCGTACTGAAAAGCAGCGCCTAACCGCCATGATGGCACAGGATCAGGTTTTGCTCCGCCAGCTCATGTGGGTCGCGGCGAATAGTCTGCggg                                                                                                                                                                                                               <  2:382506/275‑1 (MQ=255)
                                                       tGAGATTGTTGGTGACAATGCGCAAATTGC‑‑TGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGCTGGCCTTGCGATCGTCGCACGGCGGGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCCCATCGAGGCCGGGGCGAATAGTccgtgg                                                                                                                                                                                                                >  2:593431/1‑269 (MQ=255)
                                                         agaTTGTTGGTGACAATGCGCAAATTGC‑‑TGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGGGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGAACGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCTGGAGCAGGGTTTGCTGCGCCAGCCCATTGAGGTCGCGGCGAACAGTCTGCGGGc                                                                                                                                                                                                              >  1:259459/1‑274 (MQ=255)
                                                               ttgGTGACAATGCGCAAATTGC‑‑TGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCGGCCCATTGAGGTCGCGGCGAATAGTCTGCGGGCTgtagga                                                                                                                                                                                                       >  2:910018/1‑270 (MQ=255)
                                                                   tGACAATGCGCAAATTGC‑‑TGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCTAGCTATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCCCTCTTTTTCTTCGGTCTGGGTGGCCTTGCTATCGTCCCACGACTCGTTAACCGAATCGGATGGCAGCGCCGTACAGCCATCATGTCGCCTCATCAGGTTCTGCTTCCCCAGCCCATTGAGGCCGCGGCTAATAGGCGGGGGCCTGAccgccca                                                                                                                                                                                                    >  2:337312/1‑268 (MQ=255)
                                                                           cgcAAATTGC‑‑TGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTAc                                                                                                                                                                                           <  1:623038/275‑1 (MQ=255)
                                                                              aaaTTGC‑‑TGTGATTGGGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGAATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCGCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAg                                                                                                                                                                                        <  1:235834/275‑1 (MQ=255)
                                                                                  ggC‑‑TGTGATTTGGCAGTGCGTGCGCTACCGCTTCCGGCGGGGTGCCGATATCGATAAACAGAGTCGAGCCATTGGGGATTTACGCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGctc                                                                                                                                                                                     <  1:731113/273‑1 (MQ=255)
                                                                                    c‑‑TGTGATTGGGCAGTGCGTGCACTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTATGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                                                   <  1:593431/274‑1 (MQ=255)
                                                                                             ttGAGCCGTGCCTTCGCTACCGCTTCCGGCTTTGTACCGATATCAATAAACAGCGTCGAGCCATTGGGAATGTGCTCCGCCGCTTTGCGGGCGATGCGCTCTTGTGCTTCGGTCTTGGTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGAGCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                                             <  2:753687/274‑1 (MQ=255)
                                                                                                      tgcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTTCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTGGCTCCCCCCGTCCATTGAGGCCGCGGCGACTAGTCCGCGGGCTGCCGGCGAAATGCCCTACCACCTTTTCCGTACTGACATacc                                                                                                                                                                    >  2:544306/1‑273 (MQ=255)
                                                                                                      tgcgtgcgCTACCGCTTCCGGCGCGGTGCCGATATCGATAAACAGCGTCGAGTCATTGGGGACTTGCTCCGCCACCTTGCGGGCGATTCGCTCTTTTTCTTCGGTCTGGGCGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGACATGCTCTACCAGTTCTCCGGTACTGACATaa                                                                                                                                                                     >  1:65528/1‑274 (MQ=255)
                                                                                                       gcgtgcgCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCCCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGCACTGGAAGGCAGCGCCGCACCGCCATGCTGGCGCAGGATCAGGGTTTGCTCCGCCACCTCCTTGCGGTCGCGGCGAATAGTCTGCGGGCCGACGGCGAAAGGCTCTACCAGCCCTTCGGTACTGACATacac                                                                                                                                                                   >  2:629861/1‑272 (MQ=255)
                                                                                                           gcgcTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa                                                                                                                                                                                                                          <  1:701832/216‑1 (MQ=255)
                                                                                                                aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGcacgttg                                                                                                                                                                                 >  2:905388/1‑246 (MQ=255)
                                                                                                                aCCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                                                                                                                                                          >  1:867047/1‑275 (MQ=255)
                                                                                                                    cTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTAc                                                                                                                                                                             <  1:905388/252‑1 (MQ=255)
                                                                                                                       ccGGCGTGGTGCCGCTGTCGATGAGCTGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTTCGGGCGATGCGCTCTTTTTCTGCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCAGCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCGGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACTGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                                                                                                                                                    <  2:92221/274‑1 (MQ=255)
                                                                                                                       ccGGCATGGTGCCGAAATCGATAAACAGCGTCGAGGCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCGGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGATGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCaa                                                                                                                                                    <  1:337312/274‑2 (MQ=255)
                                                                                                                         ggCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGtt                                                                                                                                                  <  1:910018/274‑1 (MQ=255)
                                                                                                                                tgCCGATATCGATAAACAGCGTCGGTCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata                                                                                                                                          <  1:728491/275‑1 (MQ=255)
                                                                                                                                gggctatttCGGTAAACAGCGTGGAGCCGTTGGGGATTTGCGCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGCGCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGataata                                                                                                                                          <  2:867047/267‑1 (MQ=255)
                                                                                                                                   cGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGcg                                                                                                                                                                                                                                                                      <  2:188086/148‑2 (MQ=255)
                                                                                                                                   cGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                                                   <  1:547612/231‑1 (MQ=255)
                                                                                                                                            tAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGc                                                                                                                                                                                       >  2:226406/1‑218 (MQ=255)
                                                                                                                                                cAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCtt                                                                                                                                                                                   <  1:226406/218‑1 (MQ=255)
                                                                                                                                                    gTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTa                                                                                                                                                                              >  1:292995/1‑219 (MQ=255)
                                                                                                                                                     tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCAGTACTGACATAACCCTGCCGTTTAACCAGTTCGTTAATACCGTTGTGACGTTGtgtttg                                                                                                                      >  1:821067/1‑274 (MQ=255)
                                                                                                                                                       gggCCGTTGGTGGTTTTCTCCGCCCCTTTGCGGTCGATGCGCTCTGTTTCGTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGGACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                                                                                                                                                          <  2:360286/234‑1 (MQ=255)
                                                                                                                                                        aGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGa                                                                                                                                                                          <  2:292995/219‑1 (MQ=255)
                                                                                                                                                         gcccgtggtgttttGCTCCGTCCCTTGGCGGGCGTTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCCATCGTGCCACGGGGTTTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTc                                                                                                                  <  2:416341/263‑1 (MQ=255)
                                                                                                                                                                  gATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTaa                                                                                                                                                        >  1:834290/1‑227 (MQ=255)
                                                                                                                                                                     ttGCTCCGCCCCTTTGCGGTGGATGCGCTTTTTTTCTTCGGGCTGGGGGGGCTTGGGATCGTGCCACGGCGTGTTAACCGAACTGGGAGGCAGCGCCGCACGGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATccc                                                                                                     <  2:517127/275‑1 (MQ=255)
                                                                                                                                                                      tGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAg                                                                                                                                                    <  2:834290/227‑1 (MQ=255)
                                                                                                                                                                          ccGCCCCTTTGCGGGGGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGaa                                                                                                <  2:343236/275‑1 (MQ=255)
                                                                                                                                                                                 tttccggcggtggcgcTTTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGGGTGTTAACCAAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTAtttt                                                                                         <  2:823162/262‑1 (MQ=255)
                                                                                                                                                                                          gATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAATCGAACTGGAAGGCAGCGCCGCACTGCCATGATGGCGCAGGATCAGGTTTTGATCTGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCTGCTCTTCGGTACTAACATAACCCTGCTGTTTAACCAGTTCGATAATACCGCTGTGACGCTGTGCTTGTTTCATGTATAAACCCATGGGATTAttttcgttcttgc                                                                                >  1:475823/1‑270 (MQ=255)
                                                                                                                                                                                                           cGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCTTGCTGTTTAACCAGTTAGATTATATCGTTGTGACGTTGTGTTTGTTTCATTTCTACATCCCCGGAATTTTTTTCGTTTTCGCGCATTGGGCGAATcaac                                                               >  1:275505/1‑275 (MQ=255)
                                                                                                                                                                                                             gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGGTTAACCAGTGCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTGGCGCATTGAGGGAATcaaaa                                                              >  2:321301/1‑272 (MQ=255)
                                                                                                                                                                                                                   gTGGCCTTGCGGTCGTGCCACGGGGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCa                                                       <  1:495639/275‑1 (MQ=255)
                                                                                                                                                                                                                        cTTGCGATCGTGCCGCGGCGTGTTAACCGAACTGGAAGGCGGCGCCGCCCCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                                                  <  1:867800/275‑1 (MQ=255)
                                                                                                                                                                                                                        cTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACAAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                                                  >  1:735656/1‑275 (MQ=255)
                                                                                                                                                                                                                         gcccGACCGTGCCGCGGCGTGTAAACCGAACTCGGCGGCAACGCCGCACCGCCATGATGGCGCTGGGTCATGTTTTGCTCCGCCAGATCATTGTGGTCGCAGCAAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATGCCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                                                  <  2:134784/271‑1 (MQ=255)
                                                                                                                                                                                                                            cGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGACCAGGTTTTGCCCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGTGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACAAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATACATTTCTGGAATTATTTTCGTTTTCGCGGATTGAGCGAATCAACAAAAGCCATCGCTaa                                                >  1:134784/1‑273 (MQ=255)
                                                                                                                                                                                                                                 gTGCCACGGCGTGTTACCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCTGGTTTTGCTCCGCCAGCCGCTTGAGGTAGCGGCGAATAGGCCGCGGGCTGACGGACATTTGCCCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTACCCAGTCCGATCATACCGTCGTTCCGTTGTGTATGTTTCATTTATCAATTCCTGCCATTATTTTCGTTTTCTCGGATTGAGCCATTCAACAAAAGCCTACGCAAAACCTACg                                         >  2:544835/1‑275 (MQ=255)
                                                                                                                                                                                                                                 gTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACg                                         >  2:867278/1‑275 (MQ=255)
                                                                                                                                                                                                                                            tgtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTta                                                                                                             >  1:41383/1‑196 (MQ=255)
                                                                                                                                                                                                                                             gtTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc                              <  1:70761/274‑1 (MQ=255)
                                                                                                                                                                                                                                             gtTAACCGAACGGGAAGGCAGGGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCGGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc                              <  2:878996/274‑1 (MQ=255)
                                                                                                                                                                                                                                               tAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAt                           <  1:321301/275‑1 (MQ=255)
                                                                                                                                                                                                                                               tAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGAt                           <  1:765446/275‑1 (MQ=255)
                                                                                                                                                                                                                                                aaCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATaaa                                                                                                         <  2:41383/196‑1 (MQ=255)
                                                                                                                                                                                                                                                      aCTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGc                     >  1:292680/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                         ccGCCATGATGGCTCAGTATCAGGTTGTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATACTCTGTGTGCTGACGGAGTAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACTTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCTCGCATTGAGCGAATCAACAAAATCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATc  <  1:110106/274‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                    
CCACCGGCGAGAATGATGCGAAAATCTTCTTTTACCATCAACGTGTTAGCAACGTTGAGATTGTTGGTGACAATGCGCAAATTGC‑‑TGTGATTGAGCAGTGCGTGCGCTACCGCTTCCGGCGTGGTGCCGATATCGATAAACAGCGTCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTATAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCGATGTGTGCTCCGTTCGCCATCGACATC  >  NC_000913/3560182‑3560715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: