New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 374863565 (0.710)4 (0.050) 4/492 8.8 4.8% coding (59/663 nt) sgbH 3‑keto‑L‑gulonate 6‑phosphate decarboxylase
?NC_000913 = 3748885 97 (1.090)coding (309/663 nt) sgbH 3‑keto‑L‑gulonate 6‑phosphate decarboxylase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCGACCACTCATCACTTGAAGCCGCGCAGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3748361‑3748635
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcagcgTTGTGCCATTGCGTGGCCTTTTTCTACCGTCGCGAGCGGCGCGGCGCAGATGATGGTCATCCAGTTAGCGCCTGCGCCAAACGCCTGTTGCGCGAGCGTTTCACCAGCGTCGGCGACCTTCCAGTCAGCAACGATGATTTTGTCCGGGCACTGTTCGCGCAAGGCTTTCACCGCGCCAAGCCCTTCGTTTAAACAGAGAATGGTGCCCGCTTCGACGATATCGACGC  <  NC_000913/3748885‑3748659
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
tacagaccCCGGCTGCTTTGGTGCGGCCCTTGCCCCCCTCATCGGCACCGGGGTTTATCACAACTTCAGCTACGCCCAACGTGACTTGCCACACCCGGTGCGCACCCTGCGGCCAGAAATGACCACCCATCGGCTTTACCAAAAAAAATATCAACGTTATCCGCCTCTCATTGCCGCACTTCAGGGCTGACACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCGACCACTCATCACTTGAAGCCGCGCAGCG                                                                                                                                                                                                                                     <  2:893241/267‑1
                                                            CAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCGACCACTCATCACTTGAAGCCGCGCAGCGTTGTGCCATTGCGTGGCCTTTTTCTACCGTCGCGAGCGGCGCGGCGCAGATGATGGT                                                                                                                                                                            >  1:153438/1‑272
                                                             AACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCGACCACTCATCACTTGAAGCCGCGCAGCGTTGTGCCATTGCGTGGCCTTTTTCTACCGTCGCGAGCGGCGCGGCGCAGATGATGGTCCTC                                                                                                                                                                        >  1:867855/1‑275
                                                                TTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCGACCACTCATCACTTGAAGCCGCGCAGCGTTGTGCCATTGCGTGGCCTTTTTCTACCGTCGCGAGCGGCGCGGCGCAGATGATGGTCATC                                                                                                                                                                        <  2:153438/272‑1
                                                                                    CTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCGACCACTCATCACTTGAAGCCGCGC                                                                                                                                                                                                                                         >  1:218095/1‑187
                                                                                        CGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCGACCACTCATCACTTGAAGCCGCGCAGCG                                                                                                                                                                                                                                     <  2:218095/187‑1
                                                                                                                                                                                                                                   ACTCCTGCAACTGGCCCTGGACCACTCATCACTTGAAGCCGGGCAGCGTTGTTCCATTGCGTGGCCTTTTTCTCCCGTCGCGAGCGGCGCGGCGCAGAGGATGGTCATCCAGTTAGCGCCTGCGCCAAACGCCTGTTGCGCGAGCGTTTCACCAGCGTCGGCGACCTTCCAGTCAGCAACGATGATTTTGTCCGGGCACTGTTCGCGCAAGGCTTTCACCGCGCCAAGCCCTTCGTTTAAACAGAGAATGGTGCCCGCTTCGACGATATCGACGC  <  2:867855/275‑1
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
GGAAGAAACCGGCTGCTTTGGTGCGGCCCTTGCCGCCCGCGTCGGCACCGGGGTTTATCACAACTTCAGCGAAGCCCAACGTGACTTGCGACACCCGGTGCGCACCCTGCTGCCAGATATGACCGCCCATCAGCTTTACCAAAAAAAATATCAACGTTATCAGCATCTCATTGCCGCACTTCAGGGCTTTCACGCCCGCATTAAGGAGCACACATTATGAGCCGACCACTTCTGCAACTGGCCCTCGACCACTCATCACTTGAAGCCGCGCAGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3748361‑3748635
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcagcgTTGTGCCATTGCGTGGCCTTTTTCTACCGTCGCGAGCGGCGCGGCGCAGATGATGGTCATCCAGTTAGCGCCTGCGCCAAACGCCTGTTGCGCGAGCGTTTCACCAGCGTCGGCGACCTTCCAGTCAGCAACGATGATTTTGTCCGGGCACTGTTCGCGCAAGGCTTTCACCGCGCCAAGCCCTTCGTTTAAACAGAGAATGGTGCCCGCTTCGACGATATCGACGC  <  NC_000913/3748885‑3748659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.