BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,875,525	G→T	10.5%	V30F (GTT→TTT)	yidX →	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,875,525	0	G	T	10.5%	336.3 / 7.9	123	V30F (GTT→TTT)	yidX	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base G (65/43); new base T (13/2); total (78/45)
Fisher's exact test for biased strand distribution p-value = 5.03e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.98e-01

Only 100 of 123 total aligned reads displayed.

TTTGCCGTGCGTGTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGG  >  NC_000913/3875254‑3875803

tttGCCGTGCTTGGAAAGTAGTACAAACGTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGTGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCAt                                                                                                                                                                                                                                                                                 <  2:319685/280‑1 (MQ=255)

             aatgttagtaTAAAATTATAAGAGTGGACGACAAGTTAGATCCAAAAAAAAAGAACGCCTGAAAAAATGTCTGTGGTCGGGTAGAAGTCTGAAAAGGGATCTCAGGCGGTAAACACGATGGAAAGAAGGATATGCTGTCAACGGATTAGCGAGCCGTTTAAAAGATTAATCATGAAGTTGAATTTTAAGGTATTTTTTAAGGCTTCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTAGGCTCTGGTTTCCCATCCCGCAAAGGGTa                                                                                                                                                                                                                                                                    <  2:1054549/275‑1 (MQ=255)

                   agtaCAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGATGTCGTCTGCTTTACCACAAAGGTGAGTTCAgg                                                                                                                                                                                                                                                             >  1:1156193/1‑281 (MQ=255)

                   agtaCAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGGATGCACTGGTTTCCCATACCGAAAAGGGAAGTTCAgg                                                                                                                                                                                                                                                             >  1:914781/1‑281 (MQ=255)

                            ttATATTGTTGTACTACAATTTAGTTCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGGCTTTTCCTTTAACAAAAAGAGTCGTTCAGGAAAGTAtaa                                                                                                                                                                                                                                                    >  1:928796/1‑279 (MQ=255)

                                    ttgtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACTGCAAAGGGTAGTTTAGGAAAGTATCAAT‑CGCAGt                                                                                                                                                                                                                                           >  1:26976/1‑281 (MQ=255)

                                    ttgtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGt                                                                                                                                                                                                                                           >  1:248147/1‑281 (MQ=255)

                                      gtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTATACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTCAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACTGCAAAGGGTAGTTCAGGAAAGTATCAAT‑AGCAGTc                                                                                                                                                                                                                                          >  1:328375/1‑280 (MQ=255)

                                      gtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGGTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCACACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGGAGTTCAGGGAAGTATTAAT‑CGCAGTc                                                                                                                                                                                                                                          >  2:533248/1‑280 (MQ=255)

                                      gtACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCTTTACCGAAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTc                                                                                                                                                                                                                                          >  1:664316/1‑280 (MQ=255)

                                        actaaAATTTAAAACACAAATAGAACATGGCATAAAAAAATGACCTGCGACAGGAAGAAATCTGACAAGTGATTTCAGGCGCTAAACAGTAGGAAAAAAAGATTATGCTTTCAAAGTCTTAGCTACTCGTTTAAAATATTAATCATGAAGTTGAATTTTAATGGATTGTTTAAGGCTGCCTTTTTATTCCCACTGGCGCTTATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCTGTCAGa                                                                                                                                                                                                                                       <  2:451234/281‑1 (MQ=255)

                                         ctacAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTGCCTTACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAg                                                                                                                                                                                                                                        >  1:1106881/1‑279 (MQ=255)

                                           acAATTTAGATCACAAAAAGAACAATGCATAAAAAAAAGACATGCGTCGGGAAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGacac                                                                                                                                                                                                                                    <  2:248147/281‑1 (MQ=255)

                                           aaaaattaaaacacaAAAAGAACAAAGCATAAAAAAACGACATGCGTCGGTCACAAAGCTGAAGAGGAAGTTCGAGATCTAAACAGAAGGAAAAGAAGAGTATGCTTTCAACGTCTTAGCTACTCGTGTAAAGGATTAATCATTAAGTTGACTTTGTAGCGAGTTTTTAAGGCTTCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGacac                                                                                                                                                                                                                                    <  2:914781/270‑1 (MQ=255)

                                                   gaaaacaAAAAGAAGAATGCATAAAAAAAGGACATGCGTCGGGCAGAAAGCGGAAAAGGGAAATCAGGCGCTAAACAGGAGGGAAAGAAGAGTAGGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTAAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCAc                                                                                                                                                                                                                              <  2:1106881/275‑1 (MQ=255)

                                                   aaTCACAAAAAAAACAATGCATAAAAAAATGGCATGCGTCGTGAATAAATCTGAAAAGGTATATCAGGCGCTAAAGAGAAAGGAAAGAAGAGTATGCTTTCTACGGCTTATCTACTAGTTTAAAGGATTAATCATGAAGTTGGATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCATGCTGTATCTCGTATGCTATGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCAc                                                                                                                                                                                                                              <  2:26976/278‑1 (MQ=255)

                                                    aTCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTAAGACACCATCACTg                                                                                                                                                                                                                            >  1:487337/1‑280 (MQ=255)

                                                      cacaAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACTGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGAAACAATCACGGGGc                                                                                                                                                                                                                         >  1:770721/1‑281 (MQ=255)

                                                      cacaAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCCGCAGTCAGACACCATCACTggg                                                                                                                                                                                                                          >  1:20983/1‑281 (MQ=255)

                                                         aaaaaGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATa                                                                                                                                                                                                                                                                                <  1:1065816/224‑1 (MQ=255)

                                                         aaaaaGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCa                                                                                                                                                                                                                                                                           >  1:554585/1‑229 (MQ=255)

                                                         aaaaaGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCa                                                                                                                                                                                                                                                                           <  2:554585/229‑1 (MQ=255)

                                                         aaaaaGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGAAAAGGGAAGTTCAGGCAAGTATCAAT‑CGAAGTCAGACACCATCACTGGGCTAt                                                                                                                                                                                                                      >  1:329888/1‑281 (MQ=255)

                                                               caCAATGCATAAAAAAATGACATTCGTCGGTCATAGATATGAAAAGGGATGTAAGGGGCTAAACCTGAGTGAAAGACGTGTATGCTTTCATCGGCTTAGCTACTCGTTTAAAGGATTAATCATTGAGGTGAATTTTATGTGATTTATGAAGGCTGCCGGTTTATCCCCTTTTGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGTTTGCCCATACCGCAAAGGTTAGTTAAGGAGAGTGTCGAT‑CGCAGTCAGACACCATCACTGGTCTATCGCAgg                                                                                                                                                                                                                <  2:328375/280‑1 (MQ=255)

                                                                   aTGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGTTCTGCCTTAACGAAAAGGGTAGTTCAGGAAATTATCAAT‑CTCAGTCAGACACCCTCCCTGGGCTATAGCAGGCaaa                                                                                                                                                                                                            >  1:754024/1‑281 (MQ=255)

                                                                   aTGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGATGTATCTCGTATGCTCTGGTTTCCCATACAGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCACCACTGGGCTATCCAAGGCaaa                                                                                                                                                                                                            >  2:951032/1‑281 (MQ=255)

                                                                            aaaaTGACATGCGGCGGGCAGAAATCAGAAAAGGGATATAACGAGCGAAACGGGAGGGAAAGAAGATTATACTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTaa                                                                                                                                                                                                   <  2:487337/281‑1 (MQ=255)

                                                                                    aTGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGTTACTCTTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACaa                                                                                                                                                                                           <  2:1066559/281‑1 (MQ=255)

                                                                                                  aaTCTGAAAAGGGATATCAGGCGATAAACAGGAGAGAAAAATTAGTAGGCTTTCAACGGCTATGCTACTCGTTTAAAGGCTTAATCATGAACATAAATTTTAAGGAAGTTTTTAAAGCTTCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACCCCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGtttt                                                                                                                                                                              <  2:770721/280‑1 (MQ=255)

                                                                                                     ctgggaaaggAAATCAGGCGCGAAACAAGGTGGAATGAAGAGTATGCTTTCAACGGCTTCACTACTCTTTTAAAAGATTAATAATGAAGTTGAATTATAAGGGATGTTTTAAGGATGCGGGGTTATTCCTACAGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTAGTTTACCATAACGCAAAGGGTAGTTCAGGAAATTATCAAT‑CGCAGTCAGAAACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGt                                                                                                                                                                           <  2:620270/272‑1 (MQ=255)

                                                                                                      tGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGTTCTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACATCATCCCTGTGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTa                                                                                                                                                                          >  1:920469/1‑280 (MQ=255)

                                                                                                      tGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACAGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAAGGGAACAAACGGATATGTTTTTGTa                                                                                                                                                                          >  2:819549/1‑280 (MQ=255)

                                                                                                      tGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCGTTTCCCTTACCGAAAAGGGGAGTTCAGGAACGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAATAGGCTACGTTTTGGTa                                                                                                                                                                          >  1:359809/1‑280 (MQ=255)

                                                                                                      tGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTCAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGGAAAAGATAGTAATGGAACCAAAGGCTATGTTTTTGTa                                                                                                                                                                          >  1:109221/1‑280 (MQ=255)

                                                                                                           aGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTCGTTTGCTAGACAGAAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGATATCGCATGCTAACGATAGTAATGGAACAAAAGGAAATGTTTTTGTTGGGCaa                                                                                                                                                                    >  1:1212581/1‑281 (MQ=255)

                                                                                                                tatTAGGCGCTAAACAGGAGGGAAAGAAGAGGATGCTTTAAACGGCTTAGCTATTCTTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTGTGAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGc                                                                                                                                                                <  1:819549/280‑1 (MQ=255)

                                                                                                                      tcgttAAAGAGGGTGGGAAAAAGAGTGTTCATTCAACGGCTTTGCTGCGCGTTTAAAGGATTAATCTGTAAGTTGAATTTTAAGGGATTTGTAAAGGCAGCCGGTTTATTCCCACTGGCGCTGATGCTTTCATGGTGTAACTCGTATGCGCTGGTGTCCCATACCTCACATGGTATTTCTGGAAAGTATCAAT‑CGCATTCAAACTTCATCCCTGGGCTATCGCAGGTAAAATATAGTAATTGAACAAAAGGCTATGTTTTTGTAGGTGATTCGCTGAAt                                                                                                                                                           <  2:329888/275‑1 (MQ=255)

                                                                                                                      acgatAAACAGGAGGGGAAGAAGGGTATGTGTTCAACGACTTAGCTACCTGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCAGGTTTATTCCCGCTGGCGCTGATGCTTACAGGCTGTATCTGGGATGATCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCGATGTTTTTGTAGGGGAATCGCTGGAt                                                                                                                                                           <  2:218977/275‑1 (MQ=255)

                                                                                                                          aaaaCAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTCTTAAGCCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTCTGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCACT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCCAAATAGAGTCATGGAACAAAAGCCTATGTTCTTGTAGGGGAACCGCTGGATTCCCtt                                                                                                                                                     >  2:1000260/2‑281 (MQ=255)

                                                                                                                            aaCAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGAAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTAt                                                                                                                                                   >  1:36921/1‑281 (MQ=255)

                                                                                                                                ggaggGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCaa                                                                                                                                                                                                             >  1:508291/1‑219 (MQ=255)

                                                                                                                                ggaggGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCaa                                                                                                                                                                                                             <  2:508291/219‑1 (MQ=255)

                                                                                                                                   gggAAAGAAGGGTATGCTTTCAACGGCTTAGATGATCGTTGAAAGGTTTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGACGGTTTAGTCCCGCTGGCGCAGATGCTTGCAGGCTGGATCTCGTATGATCTGGTTTCCCATGCCGCAAAGGTTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCGGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGa                                                                                                                                             <  2:109221/280‑1 (MQ=255)

                                                                                                                                    ggAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAggg                                                                                                                                                                                                                                                                      >  2:847834/1‑159 (MQ=255)

                                                                                                                                    ggAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAggg                                                                                                                                                                                                                                                                      <  1:847834/159‑1 (MQ=255)

                                                                                                                                    ggAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGGTTTGCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑TGCAGTCAGACACCATCATTGGGCTATCGCAGGCAAAAGAAAGGAATGGAACAAAAGGATAGGTTTTTGTAGGGGAATCGCTGGAATACCTTATTACCGATg                                                                                                                                           >  1:1115227/1‑281 (MQ=255)

                                                                                                                                      aaaGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATg                                                                                                                                                                                                 >  2:321106/1‑225 (MQ=255)

                                                                                                                                      aaaGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATg                                                                                                                                                                                                 <  1:321106/225‑1 (MQ=255)

                                                                                                                                      aaaGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCACAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTAACTTATCACTGATGGt                                                                                                                                         >  1:609978/1‑281 (MQ=255)

                                                                                                                                          aagaGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCAGGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAATCCGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGt                                                                                                                                         <  2:20983/278‑1 (MQ=255)

                                                                                                                                              gTATACTTTCGACGGCTTAGCTAGTCGTTTAAGGGTTTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTTCCGGTTTATTCCCACTGGCGATGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGa                                                                                                                                 <  1:533248/281‑1 (MQ=255)

                                                                                                                                               tATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑Cg                                                                                                                                                                                                                                               <  2:1005633/170‑1 (MQ=255)

                                                                                                                                               tATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑Cg                                                                                                                                                                                                                                               >  1:1005633/1‑170 (MQ=255)

                                                                                                                                               tATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCATGCAAAAGATAGTACTGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGATGGATTACATTATCACTACTGGTGCCGTTGAc                                                                                                                                >  1:1314305/1‑281 (MQ=255)

                                                                                                                                                aTGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTGCCATACCGAAAAGGGTAGTTCAGTAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTACGGGAACAAAAGGCTATGTTTTTTTAGGGGAATCGCTGGCTTACCTTATCAATGATGTTGCCGATGACa                                                                                                                               >  1:364643/1‑281 (MQ=255)

                                                                                                                                                aTGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGGTTTCCTATACAGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGTATCGCTGCATTACCTTACCACCGATGGTGCCGATGACa                                                                                                                               >  1:183956/1‑281 (MQ=255)

                                                                                                                                                    tttCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCTTACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTTTCACTGATGGTGCCGATGACATc                                                                                                                             >  1:1080665/1‑279 (MQ=255)

                                                                                                                                                    tttCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCa                                                                                                                                                                                                                                                                           >  1:1120517/1‑138 (MQ=255)

                                                                                                                                                    tttCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATc                                                                                                                             >  2:924623/1‑279 (MQ=255)

                                                                                                                                                       cAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCa                                                                                                                                                                                                                                         <  2:667438/168‑1 (MQ=255)

                                                                                                                                                       cAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCa                                                                                                                                                                                                                                         >  1:667438/1‑168 (MQ=255)

                                                                                                                                                       cAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTTTGCTTGAAAAAAAAAAGTAGATCAGGAAAGTACCAAT‑CGCACTCAGACACCATCACTGGGATATCGCAGGCACAAGATAGCAATGGAATCAAAGGATATGTTTTTGTAGGGGAATTGCTGGCTTACATTATCACTGATGGGTCCGATGACATCGTTaa                                                                                                                        >  1:1341065/1‑281 (MQ=255)

                                                                                                                                                                aGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGCTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGATGGATTACCTTATCACTGATGGTTCCGATGACATGGGTAAGATGCCCa                                                                                                                >  2:544004/1‑280 (MQ=255)

                                                                                                                                                                      tCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCACGCAAAAGATAGTAATGGAACAAAAGGCTATCTTTTTGTAGGGGAATCGCTGGATTACCTTATTATTGATGGTGCACATGACATCGTTAACATGCCCAATGATcc                                                                                                         >  1:604680/1‑281 (MQ=255)

                                                                                                                                                                       cGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATcc                                                                                                         >  1:385536/1‑280 (MQ=255)

                                                                                                                                                                           tAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTAGCTCGTATGCTCTGGTTTCCCCTACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTAAGACACCATCACTGCGCTATCGCAGGCAAACGATAGTAATGTAACAAAAGGCTATGTTGTTGCAGGGGAATCGCTGGATTACCTTCTCACTGATGCTCCCGATGAACTTGTTAAGATCCTCAATGATCCAGCa                                                                                                     >  2:330681/1‑280 (MQ=255)

                                                                                                                                                                                  ttAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCg                                                                                             >  2:85431/1‑281 (MQ=255)

                                                                                                                                                                                  ttAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATAGATGGATTAACTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATACAGCACTTAACCg                                                                                             >  1:1083363/1‑281 (MQ=255)

                                                                                                                                                                                  ttAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTAAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCCTATCACTGATGGTGCCGATGCCATCGTTAATAGGCTTAATGATACAGCACTTAAcca                                                                                             >  1:793944/1‑280 (MQ=255)

                                                                                                                                                                                  ttAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCATTCATACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGTTCACTTCGTTAAGATGCTCAACGCTCTAGCACTTACCCg                                                                                             >  1:1084512/1‑281 (MQ=255)

                                                                                                                                                                                  ttAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATg                                                                                                                                           >  1:241186/1‑235 (MQ=255)

                                                                                                                                                                                  ttAATCATGAAGTGTAAATTGAATGGATCTTTTAAGGCTTCCGGTTTATTCCCCCTGGCGCTGATGCTTTCAGGCTTTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGTTAGTTCAGGAAAGGATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCg                                                                                             <  2:385536/281‑1 (MQ=255)

                                                                                                                                                                                   tAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTCTGCCTTAAAAAAAAAGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGAAAACCTCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAAGCGCTGGATTACCTTACCACTGATGGTGCCGATGAAAGCGTTAAGATGCGTAATGAGCCAGCACTTAACCgg                                                                                            >  1:1277771/1‑281 (MQ=255)

                                                                                                                                                                                   tAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCGTCTGCCTGACAGAAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAACATGCTCAATGATCCAGCACTTAACCgg                                                                                            >  1:476134/1‑281 (MQ=255)

                                                                                                                                                                                     aTCCTGAAGTTTAAATTAAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCGCTGGCGCTTATTCTTTCAGGCTGTATCTCGTATTCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAATATCAAT‑CGCAGTCATACACCATCACAGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGca                                                                                          <  2:1080665/281‑1 (MQ=255)

                                                                                                                                                                                     aTCATTAAGTGTGATTTTGAGGGATTTTTTGAGGTTGCCGGTTTGTTCCCGATGCCTTTGATGCTTTCAGGCTGTATCTCGTATTCCCTTTTTTCCCAAACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCAGCACTGGGCTATCGCAGGCAAAAGAAAGTAATGGAACAAAAGGCTATTTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTAAATGATCCAGCACTTAACCGGca                                                                                          <  2:1233767/281‑1 (MQ=255)

                                                                                                                                                                                          gAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGTTCTCCCTTACAGAAAAGGGTAGTTACGGAAAGTAACCAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCCAAATGATTCAGCACTTAACCGGCACAata                                                                                     >  1:1072687/1‑281 (MQ=255)

                                                                                                                                                                                              ttGAATGTTAAGGGGTTTTTTAATGCTAGCGATTTAGTCACACTGGGTCTTATGCTTTCATGCTGTATCTCTTATGCTTGTGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATGCAT‑CGCAGTCAGACACCATCACAGGGCTATCGCAGGCAAAAGATAGTAATGGAAAAAAAGGCTATGTTTTTGTAGGTGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTGAATGGTCCAGCACTTAACCGGCACAATATTCa                                                                                 <  2:36921/281‑1 (MQ=255)

                                                                                                                                                                                                gAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGAGCCAGCACTTAACCGGCACAAGATTAAgg                                                                               >  2:1106537/1‑281 (MQ=255)

                                                                                                                                                                                                      tAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGATATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCg                                                                         >  1:900065/1‑281 (MQ=255)

                                                                                                                                                                                                        aGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTGTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CTCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGTTGCCGATGACATCGTTAAGATGCTCAATGATTCAGCACTTAACCGGCACAATATTCAGGTTGACGAt                                                                       >  1:169177/1‑281 (MQ=255)

                                                                                                                                                                                                        aGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTCTTTTGCTAGACCAAAAAGGGTAGATCAGGAAAGTAACAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCATGCAAGAGATAGTAATGGAACAGAAGGCTATGTTTTTGTAGGGGAATCGCTGGCTTACCTTATCACTGATGGTGCCGATGACATCGGTAAAATGCTCAATGATCCAGCCGTTAACCGGCACAATATTCAGGTTGCCGAt                                                                       >  1:128978/1‑281 (MQ=255)

                                                                                                                                                                                                                    tagCTGCCATTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTAGCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTAGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCGATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGGGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGAt                                                             <  1:153278/277‑1 (MQ=255)

                                                                                                                                                                                                                    aGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGAtt                                                            <  1:1106537/280‑1 (MQ=255)

                                                                                                                                                                                                                          cctgTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGAt                                                                       <  1:544689/260‑1 (MQ=255)

                                                                                                                                                                                                                                tATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGGGCCGATGACATCGTTAAGATGCCCAATGATCCAGAACTTAACGGGCACAATATTCAGGTTGCCGGGGACGCAAGATTTGTTTTAAATGCg                                               >  2:1242245/1‑281 (MQ=255)

                                                                                                                                                                                                                                tATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCCGTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGCTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCg                                               >  1:67513/1‑281 (MQ=255)

                                                                                                                                                                                                                                          ggCGCTGATGCTTTCAGGCTGTATCTCGTATGCCCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCCCTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGa                                     >  1:347641/1‑281 (MQ=255)

                                                                                                                                                                                                                                            cgcTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGaa                                    >  1:822635/1‑280 (MQ=255)

                                                                                                                                                                                                                                            cgcTAATGCTTTCAGGCTGTATCTCGTATGCCGTGTTTTCCTATAACGAAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAAACGGAACAATATTCAGGTTGCCGACGACGCAAGATTTTTTTTAAATGCGGGGAAAAAGaa                                    >  1:1050832/1‑280 (MQ=255)

                                                                                                                                                                                                                                                 attgtttCATGCTGTATGTCGTATGGTCGTGTTTCCCATACCGCAAAGGTTAGTTAAGGAAATTATCAAT‑CGCATTCAGACACCATCGCTGTGCTATCGCAAGCAAAAGATATTAATGGAACAAAATGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATAGTAAAGGTGCTCAATGATCCAGCACTTAACCGTCACAATATTCAGGTTGCCGATTACTCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTAc                              <  2:609978/277‑1 (MQ=255)

                                                                                                                                                                                                                                                             tGTAACTGGTATGCTCTGGTTTCCCATACGTCGAAAGGTAGGACATGAAAGTATAGAT‑AGCAGTCAAACACCATTACTAGGCTATCGAAGACAAAAGATAGTAAGTGAACAAAAGGCTATGTTTTTGTAAGGAAATCGCTGGATTACCTTCTCACGGATGGTGCCGATGACATCGTTAAGATGCTAAATGATCCAGCACTTAACCGGAAAAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAatat                   <  2:4206/280‑1 (MQ=255)

                                                                                                                                                                                                                                                               tATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATc                                                                                                          >  2:132518/1‑191 (MQ=255)

                                                                                                                                                                                                                                                               tATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCg                 >  1:138760/1‑280 (MQ=255)

                                                                                                                                                                                                                                                               tATCTCGTATGCCCTGGTTTCCCTTACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATc                  >  1:532475/1‑279 (MQ=255)

                                                                                                                                                                                                                                                                 tttcggtggCTCTGGTTTCCCAGAACGCAAAGGTTAAGTCAGGAAAGTATCAAT‑CGCAGGCGGACACCAGCACTGGGCTATCGAAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGTGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGTTGCTCAATGATCCAGCACTTAACCGTCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCtt              <  2:900065/273‑1 (MQ=255)

                                                                                                                                                                                                                                                                    cttATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAAGGGAACAAAAGGCGATGTTTTTGTAGGGGAATCGGTGGATTACCTTATCACTGGTGGTGCAGATGAAATCGTTAAGATGCTCGATGATCAAGCACTTAACCGGCACAATAATCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCtt              <  1:1000260/276‑1 (MQ=255)

                                                                                                                                                                                                                                                                         gCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAACGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGAAGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGAACAATATTCCTTtaccacca      >  2:1175643/1‑279 (MQ=255)

                                                                                                                                                                                                                                                                          ctctGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTtactactac     <  1:924623/281‑1 (MQ=255)

                                                                                                                                                                                                                                                                             tGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTgg  >  2:794443/1‑281 (MQ=255)

TTTGCCGTGCGTGTAATGTAGTACAAACTTATATTGTTGTACTACAATTTAGATCACAAAAAGAACAATGCATAAAAAAATGACATGCGTCGGGCAGAAATCTGAAAAGGGATATCAGGCGCTAAACAGGAGGGAAAGAAGAGTATGCTTTCAACGGCTTAGCTACTCGTTTAAAGGATTAATCATGAAGTTGAATTTTAAGGGATTTTTTAAGGCTGCCGGTTTATTCCCACTGGCGCTGATGCTTTCAGGCTGTATCTCGTATGCTCTGGTTTCCCATACCGCAAAGGGTAGTTCAGGAAAGTATCAAT‑CGCAGTCAGACACCATCACTGGGCTATCGCAGGCAAAAGATAGTAATGGAACAAAAGGCTATGTTTTTGTAGGGGAATCGCTGGATTACCTTATCACTGATGGTGCCGATGACATCGTTAAGATGCTCAATGATCCAGCACTTAACCGGCACAATATTCAGGTTGCCGATGACGCAAGATTTGTTTTAAATGCGGGGAAAAAGAAATTTACCGGCACAATATCGCTTTACTACTACTGG  >  NC_000913/3875254‑3875803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`