Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,2680TC30.9% 67.4 / 19.1 44intergenic (+474/+168)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base T (15/14);  new base C (8/5);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 7.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAC  >  NC_000913/4295990‑4296551
                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                              
aCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTTGGTCGGATAAGGCGTTTAGGCCGCATcc                                                                                                                                                                                                                                                                                       >  1:482134/1‑288 (MQ=25)
    cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCATGCATCAGCGTGATTTTGGAGTTCGGATAAGGCGTTTACGACGCATCCGACa                                                                                                                                                                                                                                                                                   >  2:106446/1‑288 (MQ=255)
    cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACa                                                                                                                                                                                                                                                                                   >  1:831870/1‑288 (MQ=25)
         tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAATGCGTTTCTGCCGCATCCCACATCAACGCCTGCTGCGCCCCTTCACGCGTCTTATCAGGCCTACGCCAGACCTACCAATAGCCTTCCTGCCCTCGATTTTGTATGCCGGATAAGGCGTTTACGCCGCCTCCGCCTTCaat                                                                                                                                                                                                                                                                              >  2:124148/1‑287 (MQ=11)
                      cGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAc                                                                                                                                                                                                                                                                  >  1:2931/1‑287 (MQ=21)
                          aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCAAAACAGCGCAATAGCCTGATTTGGCGTGATTGTGTCGTTAGGCTAAGGCGTTTACGCACCAGACGACATCAATGCCTGATGCGTCGCttg                                                                                                                                                                                                                                                             >  2:825304/1‑287 (MQ=11)
                          aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACCGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAAGCCTACGCCAGCGCTCACAATCACCTGATTTAGCGTGATTTTGGAGATCGGATAAGGCGTTTACGCCGCACCCGCACTCAATGCCCGACGCTACTCttt                                                                                                                                                                                                                                                             >  2:158850/1‑287 (MQ=11)
                                     tggaagaGGCGTTTACGCCGCAGCGGACATCAACGCCGGATGCGACGCTGGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCCATAGCCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                                                                                                                                                  <  2:1006426/282‑1 (MQ=11)
                                     cgagtAAGGCGTTTACCCCGCATCCGACATCAACCCTTGGTTAGACGCTTGCCGCGTCTTATTAGGACGACGCCAGACAGCGCAATAGCCTTATTTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                                                                                                                                                  <  1:207450/284‑1 (MQ=11)
                                     cGGATAAGGCGTTAACTCCGCTTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATGTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                                                                                                                                                                                                                  <  1:1025994/288‑1 (MQ=255)
                                         tAAGGCGTTTACGCCGCCTCCGCCATCAACTCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCGGACAGCGCAATTGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGc                                                                                                                                                                                                                                             <  1:998118/289‑1 (MQ=11)
                                                  tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCATGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACAACAATGCCTGATGCGACGCTTGCCTCGTTCTATCAGGCATAccttaa                                                                                                                                                                                                                                    >  1:1009367/1‑284 (MQ=11)
                                                  tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAAACAGCGCAATATCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCTCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACACGGCAATAGCCTGATTTAGCGTGCGTTTTTGTATCGGAAACGGCGTTTACGCGAAATCCTAACACAATGCCAGATGCGCCGCTGCCCGCGTCTAATCCGGCTTatccta                                                                                                                                                                                                                                     >  2:917933/1‑283 (MQ=11)
                                                                                                                                                                        cGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGtt                                                                                                              >  1:422614/1‑289 (MQ=12)
                                                                                                                                                                                       cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa                                                                                               <  1:825304/289‑1 (MQ=255)
                                                                                                                                                                                             aTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGCCGGATCAGGCGTTTACGCCGCATGCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTAACCGTTGGTTAATTTTTCAACATCtt                                                                                          >  1:40141/1‑288 (MQ=11)
                                                                                                                                                                                                   cGCTTGCCGCTTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCGGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑Accc                                                                                  <  1:262246/289‑1 (MQ=255)
                                                                                                                                                                                                    gcttgccgcgTCTTAGCAGGCCTAGGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCGTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑Accc                                                                                  <  1:106446/282‑1 (MQ=255)
                                                                                                                                                                                                       tgccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTa                                                                              >  1:652121/4‑289 (MQ=255)
                                                                                                                                                                                                        gccgcgTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACACGGTa                                                                              >  1:313419/3‑288 (MQ=255)
                                                                                                                                                                                                          cgcgGCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGTGCGGATAAGGCGGTTATGCCGCATCCGACATCCACGCCTGATGAGACGCTTAACGAGTCTTATCAGTCCTACGCCAGACAGCGCAATATCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCCTCCGACATCAATGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCa                                                                           <  1:1006735/289‑1 (MQ=14)
                                                                                                                                                                                                            tgTCTTATCCGCCCTACCCCAGACAGCGAAATAGCCTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCaaa                                                                         <  1:464656/288‑1 (MQ=255)
                                                                                                                                                                                                             gTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCaaa                                                                         <  1:55302/288‑1 (MQ=255)
                                                                                                                                                                                                                           aagcgaGACATCGCAATATCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGTCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCt                                                          <  1:1086517/284‑1 (MQ=255)
                                                                                                                                                                                                                           aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCt                                                          <  1:1033870/289‑1 (MQ=255)
                                                                                                                                                                                                                            cGCCAGACAGCGCAATAGCGTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGAAGCTTGCCGAGTCTTATCATGCCTCTCTTAACCGTTGGTTAATTTTTCAAAATCTTACA‑ACCGGGTAACAAACCCTTCCATTAAGCt                                                          >  1:1032212/1‑288 (MQ=14)
                                                                                                                                                                                                                            cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAAcc                                                                       <  1:820026/275‑1 (MQ=255)
                                                                                                                                                                                                                            cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTc                                                         >  1:532709/1‑289 (MQ=255)
                                                                                                                                                                                                                                   cgtggcAAAAGCCTGTTTTGGATTGATTTTGTAGTTCGGATAAGGCGTTTAGGCTGCATCCAACATCAACGCCTGATGCGACGCTTACCGCTTCTTATCAGGCTTACGCCAGACAGCTCAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTTACGCCTCATCCGACATCAATGCCTGATGCGACTCTTTCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                                     <  1:978799/282‑1 (MQ=255)
                                                                                                                                                                                                                                   cAGCTCAATAGCCTGATTTAGCGTGATTTTTTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTGTCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTCAGGt                                                     <  1:824317/286‑1 (MQ=255)
                                                                                                                                                                                                                                      cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCCGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGGATGTCGGATAAGGCCTTTACGCCGCCTCCGACATCACAGCCTGATGCGAAGCTTCACGCGTCTTATCCGGCCTATCTTAACCGTTGGGTAATTTTTCAACTTCTTACC‑ACCCGGTAACCAACCCTTCACTACACCTCCGGTTCacca                                               >  2:612862/1‑286 (MQ=14)
                                                                                                                                                                                                                                      cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTTCCAAACCCTTCCATACAgtgttcccttttac                                               >  2:820026/1‑276 (MQ=14)
                                                                                                                                                                                                                                      cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAcc                                               >  1:319800/1‑289 (MQ=255)
                                                                                                                                                                                                                                                tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgcg                                      >  2:854822/1‑288 (MQ=255)
                                                                                                                                                                                                                                                   ttttgggggATTTTGTAGGTCGGTTAAGGGTGTTATGGCGCCTCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGGGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCt                                   <  2:599240/280‑1 (MQ=18)
                                                                                                                                                                                                                                                        cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCt                             >  1:720029/1‑289 (MQ=255)
                                                                                                                                                                                                                                                        cGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCATCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCCTCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTTGTTAATTTTTCAACCTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCt                             >  1:202985/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                tgtgGGGCGGATTATGCCTTTATGCCGCATCCGCCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttcttc                     <  1:341198/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                 tgaGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTGTCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTtcttct                    <  1:383004/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                       cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTACTTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCTGTGAAGCGCCTTTTGCTTGTTCTTCTCGATaa              >  1:986345/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                        ggATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATaaa             <  1:711718/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                         gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCCAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTTCCGCGCCTTTGTCTTTTTCTTCTCGATaaa             >  2:626802/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                 ttttACGCCGCCTCCGACACCAACGCGTTGTGCGACGCTTACCGCGTCTTATCAGGCGTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttctt     <  2:948409/287‑1 (MQ=21)
                                                                                                                                                                                                                                                                                    tATGCCGCATCCGACATCAAAGCCTGATGCGACGCTTAACGCGTATTATCAGGCCTACGACAGACAGAGAAATAGCCTGATCTAGCGTGATTTTGTCGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGAGTCTTCTCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAA‑TCATTACAATCCGGTATCAAACCCTTCAATCCAAATAAGTTTCACACTATGCACCGCCTTTTCCTTGTTACTCTCTATAAGACATTCTTCAc  >  2:488718/1‑288 (MQ=14)
                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                              
ACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTA‑‑CCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAC‑ACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAC  >  NC_000913/4295990‑4296551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑