Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,470,410	(T)7→6	coding (1280/1332 nt)	gtrS →	serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,470,404	0	T	.	100.0%	46.3 / NA	12	coding (1274/1332 nt)	gtrS	serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (6/6);  total (6/6)

ATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAACTTAAATGCATCATTGCCCACAACAAACC  >  NC_000913/2470150‑2470651                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                        aTCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAaataat                                                                                                                                                                                                                                    >  2:823974/1‑275 (MQ=255)                                                              atatCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg                                                                                                                                                                       <  1:823974/275‑1 (MQ=255)                                                                                              ttttATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtct                                                                                                                                       <  2:707896/275‑1 (MQ=255)                                                                                                                                         tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCTTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TCTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTCCGCGCCAATCATGGCGCGAACAAGCTATAACACTAACCTAATTTCTCTCCCCCTTAGAGGGACTATATCTCATAATAGACTTGCggca                                                                                             >  2:811744/1‑272 (MQ=255)                                                                                                                                                                  aTGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGccca                                                                                                                                                               >  2:693155/1‑183 (MQ=255)                                                                                                                                                                      gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGNCAATAGCGATNNAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCt                                                                                                                                                           <  1:693155/183‑1 (MQ=255)                                                                                                                                                                           ttttCGGGAAAGGATATAGAGATTTAAGCAATCATTTATTTAGAATGTATTTTTCATTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGTCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGt                                                          <  1:811744/275‑1 (MQ=255)                                                                                                                                                                                                          gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcg                                                                                                                                                                     >  2:848466/1‑137 (MQ=255)                                                                                                                                                                                                          gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCACGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAACtt                            >  1:462829/1‑274 (MQ=255)                                                                                                                                                                                                          gcaTTTATTTGGAATGCATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAACTCCTGATAGAATTGCGGTATTGACTATTAAATGACCTGATTCGTTGTTTATTGTAATTTCTCCTCCATCTCAAGACGATAACtt                            >  2:821019/1‑274 (MQ=255)                                                                                                                                                                                                              ttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGca                                                                                                                                                                 <  1:848466/137‑1 (MQ=255)                                                                                                                                                                                                                                    tcgACCTTTTCTTATACATAACAGTCG‑TTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAATTTTAAATTGCGCGCCAAGCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGATTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAACTTAAATGCATCATTGCCCACAACAAAcc  <  1:821019/274‑1 (MQ=255)                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                        ATCAAACATACTAACGACTATTACTAACATAAAAGACAATATATTTAAAAACATTAGATTTATATCATTGTTATTATTTTTTATTGCTTCTATTTTTATTAGAAATAATAAAATAAAGGCATCTTTATTTGTAGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGTTTATTGTAATATCTCCTCTATCTGCAGACGATAACTTAAATGCATCATTGCCCACAACAAACC  >  NC_000913/2470150‑2470651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑