Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC97.5% 230.7 / NA 79I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base C (39/38);  total (40/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132647‑2132926
                                                                                                                                            |                                                                                                                                           
ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGCTATGCAGGCTc                                                                                                                                           >  2:1070740/1‑143 (MQ=255)
   tCTTGTAGAGGGTAAACAGTTGATGTTTTTTGCCGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                        <  2:431258/143‑1 (MQ=255)
        tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATAGATATGCCGGCCCTGCGTcac                                                                                                                                   >  2:931461/1‑141 (MQ=255)
         agagGGTAAACAGCGGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGcc                                                                                                                                  <  2:1118852/143‑1 (MQ=255)
             ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCCCTGCGTCGCCtctt                                                                                                                              >  1:141173/1‑143 (MQ=255)
              gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAAGTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttc                                                                                                                             <  2:277867/143‑1 (MQ=255)
                    aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTACACGTGGAAGTACCCGGAGCATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCCCCGCGTCGCCTCTGCTACttt                                                                                                                       >  1:170022/1‑143 (MQ=255)
                       tgctatTCTGTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                    <  2:579584/140‑1 (MQ=255)
                       tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTTAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTTTTCTCCTTTCCa                                                                                                                    >  2:1320862/1‑143 (MQ=255)
                       tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                    >  2:1181262/1‑143 (MQ=255)
                       tGATATGTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                    <  2:1201057/143‑1 (MQ=255)
                          cgttgttccttGGCGCGGTCTAATCGAGGTTGGTGTTGGCGGTACAAGGTGAAGTACTCGGAGAATTTTTTCGGTGACGATGTGATCTTCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACgg                                                                                                                 <  1:1043797/133‑1 (MQ=255)
                             tttgtGCGGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTc                                                                                                              <  2:848123/143‑1 (MQ=255)
                               tttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGc                                                                                                            <  2:1068735/143‑1 (MQ=255)
                                    tGGCGCGGTCGCATTTGTCTTTGTCCTTGCGGTATAGGTGGCCGTACCCCGTGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGACATGCCGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAc                                                                                                       <  1:1068177/143‑1 (MQ=255)
                                       cgcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc                                                                                                    <  2:407973/143‑1 (MQ=255)
                                        gcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTAGGTGACGAGGTGATCTGCATTTAACCGTGATTGATATGAAGGCTCAGCGTCGCCTCCTCCACGCTCCACGGTTCGCCGTACTgca                                                                                                   >  1:1290457/1‑143 (MQ=255)
                                         cgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGGTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGGCGCCGCTTCTACTTTCCACGGTTCGCCGCACGccac                                                                                                  >  1:54242/1‑143 (MQ=255)
                                         cgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac                                                                                                  <  2:53466/143‑1 (MQ=255)
                                         cgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGAGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTGGCCTCTTCTACTTTCCACGGTTCGCCGTACTgcac                                                                                                  >  1:487441/1‑143 (MQ=255)
                                          gtgtgtaTTTGTCTTTGTGCTGGCGGTTACAGTTGAAGTACCCGGAGAATTTTTTCGGTTACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc                                                                                                 <  2:536546/137‑1 (MQ=255)
                                           gTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCGACGGTTCGCCGTACTccacca                                                                                                >  1:22706/1‑143 (MQ=255)
                                              gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCAGTACTacaccacca                                                                                             >  1:178765/1‑143 (MQ=255)
                                               aaTGTGTCTTTGTGTTGGCGGTTAGAGGGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCTGGCTTTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCACCAt                                                                                            <  1:289419/143‑1 (MQ=255)
                                                aTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc                                                                                           >  2:1133444/1‑143 (MQ=255)
                                                   tGTCTTTGTGCTTGCGGTAAAAGTGGTAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGc                                                                                        <  1:1009646/143‑1 (MQ=255)
                                                   tGTCTTTGTACTGGCGGTCATATTGTAAGTCCCCGGAGATTTTTTCCGGTGACGAGGTTATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCTCGGTTCTCCGTACTCCACCACCTTCCGc                                                                                        <  1:392202/143‑1 (MQ=255)
                                                    gaccttGTGCCAGCGGTGAAAGTCGAAGTCCCCGGAGGATGTCTTCGGAGACGAGGTCATCTGCATTTCACCGTGATCGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa                                                                                       <  2:159067/139‑1 (MQ=255)
                                                       tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa                                                                                    >  2:1107101/1‑143 (MQ=255)
                                                       tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGGTCGCCGTACGCCACCACCATCCCCAGGa                                                                                    >  2:797547/1‑143 (MQ=255)
                                                       tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGa                                                                                    >  1:471752/1‑143 (MQ=255)
                                                         tgtgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCCGTCTCCCCCGCCATCCGCACGAAg                                                                                  >  2:892668/1‑143 (MQ=255)
                                                         cgtgCTGGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAg                                                                                  <  2:112463/142‑1 (MQ=255)
                                                           tgCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAt                                                                                <  2:232032/143‑1 (MQ=255)
                                                           ggCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATAGGCAGGCTCTGCGTCGCCTCCTCTAATCTCTAAGGTTGTCCGTACTCCACCACCATCCGCAGGAAGAt                                                                                <  2:930268/142‑1 (MQ=255)
                                                              ttGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGAAGATGTc                                                                             >  2:1265371/1‑143 (MQ=255)
                                                               tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg                                                                            >  2:941540/1‑143 (MQ=255)
                                                                gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCGACCACCATCCGCAGGCAGATGCCGt                                                                           >  2:1147615/1‑143 (MQ=255)
                                                                gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGt                                                                           >  2:1143698/1‑143 (MQ=255)
                                                                 cGGTAAAAGTGGAAGTACCCGGAGAATTTTTTTTTTTTTTTTTTGATCTGCATTTCACCGTGATTGATATTCTGGCTCTTCGTCGCCTCTTCTACTTTCCTCGCTTCTCCGTTCTCCACCACCATCCGCACGACAATGTcttt                                                                          >  1:1095096/1‑140 (MQ=255)
                                                                  ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAAGTCGGaa                                                                         >  1:1142316/1‑143 (MQ=255)
                                                                     aaaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATcc                                                                      >  2:841366/1‑143 (MQ=255)
                                                                       aaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                   >  2:456117/1‑80 (MQ=255)
                                                                       aaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                   <  1:456117/80‑1 (MQ=255)
                                                                        aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGGAATCCtgt                                                                   >  1:1125710/1‑143 (MQ=255)
                                                                            gAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCg                                                               >  2:535591/1‑143 (MQ=255)
                                                                                 aCCCGGAGAATTTTTTCGGTCACGTGGTGATCTGGATTTCTCCGTGAGTGATATGCAGGCGCTGCGTCGCCTCTTCTACTTTCCTCGGTTCGCCGTACTCCACCACCATCCGCAGGAGGATGTCGTAATCCTGTGCCGCTTTc                                                          <  1:69174/143‑1 (MQ=255)
                                                                                  cccggggcgttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATGGATATGCAGGCTCTGCGTCGCTTCTTCTACTGTCCACGGTCCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                         <  1:871315/134‑1 (MQ=255)
                                                                                   ccGGAGAATTCTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAg                                                        <  2:1227725/143‑1 (MQ=255)
                                                                                     ggAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                      <  1:739108/143‑1 (MQ=255)
                                                                                     ggAGAATTGTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATACGCGTTGTTTCTTCGACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                      <  2:626100/143‑1 (MQ=255)
                                                                                       cgatgtttttCGGTGACTTGGTGATCGGAATCTGTCCTTGGTTGATATTGCGGCGCTGCGGCGCCTCTTGGACTTTCTACGGTTCGCCGGTATCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                    <  1:174242/138‑1 (MQ=255)
                                                                                       agcaTTTTTGCGGTGACGCGGTGATCTGCATTTCACCGTGATTGATATGCTGGCTCTGCGTCTCCTCTTCTACTGTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                    <  1:1172154/140‑1 (MQ=255)
                                                                                       agaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCg                                                    <  1:941540/143‑1 (MQ=255)
                                                                                            tttttCGGTGACGAGGTGTTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                               <  1:1177624/143‑1 (MQ=255)
                                                                                             tgttCGGTGCCGATGTTGGCTGCACTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg                                              <  1:206045/141‑1 (MQ=255)
                                                                                                  ggTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTGTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaa                                         <  2:517109/143‑1 (MQ=255)
                                                                                                   gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                        <  2:383165/143‑1 (MQ=255)
                                                                                                    tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTCATCCTCTGCCGCTTTCCGTTCGCTAACGcacaaac                                       >  1:71402/1‑143 (MQ=255)
                                                                                                       cGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTTGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAATAct                                    <  1:1181262/143‑1 (MQ=255)
                                                                                                        gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACActc                                   >  2:748918/1‑143 (MQ=255)
                                                                                                        gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGAGATCGaacaaacgatc                                   >  2:1292290/1‑139 (MQ=255)
                                                                                                        gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCAACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTCCAGTTCGGAATCGAACAAacacac                                   >  1:1197616/1‑141 (MQ=255)
                                                                                                              aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGGCGTACTCCACCACCATCCGCAGGAAGATGTCGTACTCCTGTGCCGCTTTCAGTTCGGGATCGAACAAACACCCTTTGac                             >  2:973369/1‑142 (MQ=255)
                                                                                                                ctccATTTCACCTGGTTTGATGTGCGTGCTCCGCGGCGGCTCTTCTACTTTCCACGGTGCGCCGTACTCCACCACCCTCCGCAGGAAGATGTCGTAAGCCTGGTCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAAc                           <  1:740788/140‑1 (MQ=255)
                                                                                                                cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTCTCCTAGCCTGTTCGGTATCGAACAAACACTCTTTGacac                           >  1:594793/1‑143 (MQ=255)
                                                                                                                 tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACCCTCtttccaccg                          >  2:932593/1‑137 (MQ=255)
                                                                                                                 tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACAACCATCCGCAGGAAGATGTCGTAATCGTGTGCCGCTTTCAGTTCGGTATCGCACAAACACTCTTTGACACg                          >  1:1275907/1‑143 (MQ=255)
                                                                                                                      ttCACCGTTATTTTTATGCAGGCTCTTCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATg                     <  2:366107/143‑1 (MQ=255)
                                                                                                                       tCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                         >  2:391665/1‑106 (MQ=255)
                                                                                                                       tCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                         <  1:391665/106‑1 (MQ=255)
                                                                                                                             tgattgatAGGCGGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCCCCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACGAACACTCTTTGAAACGCCATGCCCAGGt              <  1:962197/143‑1 (MQ=255)
                                                                                                                                   atatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGCAAAGAc        >  1:1113739/1‑143 (MQ=255)
                                                                                                                                   atatGCAGGCGCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc        <  2:1048876/143‑1 (MQ=255)
                                                                                                                                    tatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTCTCGAACAAACACTCTTTGCACCGCCACGCCCAggcaccgacc       >  1:184088/1‑135 (MQ=255)
                                                                                                                                       gCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATcc                                                                                          <  2:142456/57‑1 (MQ=255)
                                                                                                                                       gCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATcc                                                                                          >  1:142456/1‑57 (MQ=255)
                                                                                                                                        cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGt   >  2:1230298/1‑143 (MQ=255)
                                                                                                                                         aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt  >  2:195139/1‑143 (MQ=255)
                                                                                                                                            |                                                                                                                                           
GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132647‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: