Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,950,341 Δ1 bp coding (21/99 nt) ilvL → ilvG operon leader peptide

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,3410G.97.0% 277.2 / ‑0.5 67coding (21/99 nt)ilvLilvG operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base . (27/38);  total (28/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGAC  >  NC_000913/3950204‑3950478
                                                                                                                                         |                                                                                                                                          
ttATATTGAAATGATTAAACGCATCATATGAATCCGCCAACCAAGATCTTTTACTATTTACAAAACCTATGGTAACTCTTTAGGCATACCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGc                                                                                                                                      <  1:244840/143‑1 (MQ=255)
ttATATTGAAAGTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGc                                                                                                                                      <  2:155206/143‑1 (MQ=255)
   aatTGAAAGTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTg                                                                                                                                   <  1:56351/142‑1 (MQ=255)
    atTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTgg                                                                                                                                  >  2:702723/1‑143 (MQ=255)
      tGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTc                                                                                                                                <  1:361875/143‑1 (MQ=255)
          attattAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACACGACGCAAGACCAGACGATATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGa                                                                                                                            <  1:1038327/143‑1 (MQ=255)
             attaAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTCGCCTGGTCGTGATTa                                                                                                                         >  1:131869/1‑143 (MQ=255)
             attaAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTa                                                                                                                         <  1:260095/143‑1 (MQ=255)
               taAACGGATCAGAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACGCTTTAGACATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGc                                                                                                                       <  2:635504/143‑1 (MQ=255)
               taAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTATTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCAACGAGGATTAGCCgggaccggaagagc                                                                                                                       >  2:63719/1‑132 (MQ=255)
                       tcatAAAAGTCGGCATAAAGATAGCTTGTGCTATTTACAAAGCGTATGGTAACTCTTTAGGCATTCCTGCGAGCAAGATGCAAGGAAAGACAAAATGACGGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCgtggtggt                                                                                                               <  2:496802/143‑1 (MQ=255)
                          tAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAACCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGat                                                                                                            >  1:847499/1‑143 (MQ=255)
                           aaaaaTCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTACCTCTTTAGGCATTCCTTCGAACAAGATGCAGGCGAAGACTAAATGTTAGTCCTGTTACGACTATTACCCTGGTCGTGATTAGCGTGGTGGAGata                                                                                                           >  1:681392/1‑142 (MQ=255)
                            aaaaTCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGatta                                                                                                          <  1:483257/143‑1 (MQ=255)
                             aacttctccAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCTAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGattat                                                                                                         <  2:935381/136‑1 (MQ=255)
                                tCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACATGATGCAAGAAAAGACAAAATGACAGACCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATtattat                                                                                                      <  2:1043812/143‑1 (MQ=255)
                                tCGGCCAAAAAAGATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATtattat                                                                                                      <  2:573841/143‑1 (MQ=255)
                                 cGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATc                                                                                                     <  1:712365/143‑1 (MQ=255)
                                   gCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCCAGAAAAAACATGAAGACTGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATGAGccc                                                                                                   >  1:1084669/1‑143 (MQ=255)
                                      aaaaaaTATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCAcc                                                                                                >  1:936473/1‑143 (MQ=255)
                                       aaaaaTATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGCACCTCACTCAAAAAAATACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCg                                                                                               <  1:505208/143‑1 (MQ=255)
                                            tatCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCgg                                                                                          <  1:253278/143‑1 (MQ=255)
                                              tCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATTACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCgggg                                                                                        <  2:886698/143‑1 (MQ=255)
                                                ttGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCt                                                                                      >  1:698482/1‑143 (MQ=255)
                                                ttGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCt                                                                                      >  1:160131/1‑143 (MQ=255)
                                                ttGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAACATGGTCGTAATTAACGTGTTGGTGATTATGATCACACCGtaagatgat                                                                                      >  2:579238/1‑135 (MQ=255)
                                                   tACTATTTACAAAACCTATGGTAACTCTTTAGGGGTTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCCACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGCTTATTATCCCACCGTGCGGGGCTGCa                                                                                   >  2:1012909/1‑143 (MQ=255)
                                                   tACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCGCTTCTACGACTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGCGGCTGCa                                                                                   >  2:884793/1‑143 (MQ=255)
                                                      tATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACtt                                                                                >  2:537862/1‑143 (MQ=255)
                                                       aTTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTg                                                                               >  2:696693/1‑143 (MQ=255)
                                                         ttACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGa                                                                            <  2:160131/143‑1 (MQ=255)
                                                         ttACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGa                                                                            <  2:698482/143‑1 (MQ=255)
                                                         ttACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGa                                                                            <  1:1063513/143‑1 (MQ=255)
                                                            cAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGGGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGa                                                                         <  2:1006768/143‑1 (MQ=255)
                                                            cAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGa                                                                         <  2:235185/143‑1 (MQ=255)
                                                             aaaaCCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAg                                                                        >  1:827681/1‑143 (MQ=255)
                                                             aaaaCCTATGGGAACGGTGGAGGCATCCCGGCGAACAAGATGCAAGAAAAGACAAAATGACAGCTCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGGGGTGATTATTATCCCATCGTGCGGGGCTGCACTTGGACGAg                                                                        <  1:666618/143‑1 (MQ=255)
                                                               aaCCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGAGGTGGTGCTTACTATCCCACCGTGCGGGGCCGCACTTGGACGAGGa                                                                      >  2:482756/1‑143 (MQ=255)
                                                                  cTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTAAGAGTATTAGCCTGGTCGTAATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGaagg                                                                   >  2:634809/1‑141 (MQ=255)
                                                                      ggTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCtt                                                               >  1:742916/1‑143 (MQ=255)
                                                                      ggTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCtt                                                               <  2:742916/143‑1 (MQ=255)
                                                                         aacgcgggaGGGATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGGGATTAGCGTGGTGGTGAATACTATCCCATCATGCGGGGCTGCAATATGGCGAGGAAAGGCTTaga                                                            <  2:441030/135‑1 (MQ=255)
                                                                          aCTCTGTAGGCAGTCCTTCGAACACGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTATCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGCACGAAGAAAGGCTTagag                                                           <  2:603710/143‑1 (MQ=255)
                                                                           ctctGTAGGCACGCCTTAGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAgaga                                                          <  2:562319/143‑1 (MQ=255)
                                                                                   gCATTCCCTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCt                                                  <  2:1084669/143‑1 (MQ=255)
                                                                                      ttCCTTCGAACAAGATGCAAGAAAAGACAAAATGACCGCCCTTCTACGAGTATTAGCATGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCCGGGATGCACGTGGCCGAGGAAAGGCTCAGCGATCCAGCCGTaa                                               >  2:468989/1‑143 (MQ=255)
                                                                                       tCCTTCGAACAAGATGCAAGAAAAGACAGAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGAGATCCGCATGGCGGTGCTTATTATCACACCGTGCGCGGCTGCACTTGGGCGAGGACAGGCTCAGAGCTCAAGCATTAAc                                              >  2:886521/1‑143 (MQ=255)
                                                                                          ttCGAACAAGATGCAAGAAAAGACAACATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGAGTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGaa                                           <  1:299611/143‑1 (MQ=255)
                                                                                          ttCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGaa                                           >  2:815799/1‑143 (MQ=255)
                                                                                                 aaGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGAc                                    >  1:607220/1‑143 (MQ=255)
                                                                                                    tgccAAGAAGCGACGAAAGGACAGCCCTTCTACGCGTAGTAGCCTGGTCGGCATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGAcccc                                 <  2:889729/140‑1 (MQ=255)
                                                                                                    aTGCAAGAAAAGACAAAATGACATCCCTTCTACGAGTATTAGCCGGGTCGTGATTAGCGTGGTGGTGATTATTAGCCCACCGTGCGGGGCCACCATAAAAATATTAAAGGTTTATAGATCAAGCCTTAACGAACTAAGAcccc                                 <  1:207976/143‑1 (MQ=255)
                                                                                                     tacAAGAAAAGACAAAATGACGGCCCTGCTACGTGTATTAGTCTGTTCGTGATTAGCGTTGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAATCCTTAACGAACTAAGAccccc                                <  2:405208/141‑1 (MQ=255)
                                                                                                       cAAGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGc                              <  1:4004/143‑1 (MQ=255)
                                                                                                        aaGAAAAGACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGACTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATGAAGCCTTAACGAACGAAGACCCCCGCa                             >  2:963686/1‑143 (MQ=255)
                                                                                                           aacaGACAAAATGACCGCCCTTCCACGAGTAGTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCg                          <  1:124578/140‑1 (MQ=255)
                                                                                                               gACAAAATGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAg                      <  2:151909/143‑1 (MQ=255)
                                                                                                                  aaaaTGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCACGCCTTAACGAACTAACACCCCCGCACCGCCAGGTc                   >  1:885230/1‑143 (MQ=255)
                                                                                                                  aaaaTGACAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTc                   <  2:59767/143‑1 (MQ=255)
                                                                                                                        acggctcTTGTACGAGTGTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCggggg             <  2:448900/137‑1 (MQ=255)
                                                                                                                        aCAGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGCAACAGGGTGATGTTTGTGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCggggg             >  1:1082545/1‑143 (MQ=255)
                                                                                                                          aGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGGCCCGAAAGGACCAGCGGtg           >  2:317063/1‑142 (MQ=255)
                                                                                                                          aGCCCTTCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGtt           >  1:138279/1‑143 (MQ=255)
                                                                                                                            cacgtCTAGGAGTATTGGCCTGGTCGTGATGAGGGGGGTGGTGATTATTATCCCACCGTGGGGGGTTGGCTCGGTACAACGGTGGGCTTATCCATCAACCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGtttt         <  1:1088091/139‑1 (MQ=255)
                                                                                                                                tCTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGtttttttt     <  1:531164/143‑1 (MQ=255)
                                                                                                                                 cTACGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTg    >  2:113381/1‑143 (MQ=255)
                                                                                                                                   aCGAGTATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGAc  <  1:1030856/143‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                          
TTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAACCTATGGTAACTCTTTAGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGAC  >  NC_000913/3950204‑3950478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: