Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	66 [0]	[1] 66	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	63 (1.040)	49/274	0.2	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

ACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGG  >  NC_000913/1300560‑1300839                                                                                                                                           |                                                                                                                                              acagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataat                                                                                                                                           <  1:340515‑M2/5‑1 (MQ=255)     ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATc                                                                                                                                       >  1:141162‑M2/135‑143 (MQ=255)       agttataaggatttcgttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCaa                                                                                                                                     <  2:263897‑M2/11‑1 (MQ=255)        gttataaggagttccttaactgcttctcctcaccatcatgttattgtcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAt                                                                                                                                    <  1:641576‑M2/12‑1 (MQ=255)        gttataagaattaccttaactgcttcgcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAt                                                                                                                                    <  1:614189‑M2/12‑1 (MQ=255)         ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                   <  1:185039‑M2/13‑1 (MQ=255)         ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAAtt                                                                                                                                   <  1:778444‑M2/13‑1 (MQ=255)           ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGt                                                                                                                                 <  2:981702‑M2/15‑1 (MQ=255)               ggagttccttaactgcttctcctcaccgtcatgttattttcggcacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                             <  2:725205‑M2/19‑1 (MQ=255)                gatttccttaactgcttctcctcaccatcatgttattttcgccacatcatactcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttgctagaggaaagtgctaaATAATAATCAATTGTTAAat                                                                                                                            >  2:408532‑M2/124‑143 (MQ=255)                gatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCACTTGTTAAat                                                                                                                            >  2:273802‑M2/124‑143 (MQ=255)                gatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAat                                                                                                                            >  1:923958‑M2/124‑143 (MQ=255)                  tttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagcataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAatta                                                                                                                          >  1:425409‑M2/122‑143 (MQ=255)                      cttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgt                                                                                                                      <  2:558997‑M2/26‑1 (MQ=255)                       ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                     >  1:745745‑M2/117‑143 (MQ=255)                       ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgcggaatagtaagggaaatgatagtattaattccactgcgtttgggagaggaaaatggcgtATAATAATCAATTGTTAAATTATggtg                                                                                                                     >  2:120221‑M2/117‑143 (MQ=255)                         aatttgttttcctcaccatcctgttagtttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCa                                                                                                                   <  2:1017405‑M2/29‑1 (MQ=255)                            tgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAttt                                                                                                                <  2:632356‑M2/32‑1 (MQ=255)                                   ccccaccagcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCactact                                                                                                         <  1:473741‑M2/39‑1 (MQ=255)                                     tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                       >  1:392386‑M2/103‑143 (MQ=255)                                     tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                       <  1:331515‑M2/41‑1 (MQ=255)                                       accatcatgttattttcgccacatcataatcctgggcttgctgaagaataattggaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGaa                                                                                                     >  1:917645‑M2/101‑143 (MQ=255)                                             atgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTaa                                                                                               <  1:189867‑M2/49‑1 (MQ=255)                                               gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                             <  1:753300‑M2/51‑1 (MQ=255)                                                   ttttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                         <  2:923958‑M2/55‑1 (MQ=255)                                                       cgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGa                                                                                     <  1:834282‑M2/59‑1 (MQ=255)                                                        gccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                    <  1:371045‑M2/60‑1 (MQ=255)                                                            catcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGAc                                                                                <  1:924521‑M2/64‑1 (MQ=255)                                                                 taatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCt                                                                           >  2:985784‑M2/75‑143 (MQ=255)                                                                  aatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACGTGCtt                                                                          >  1:29611‑M2/74‑143 (MQ=255)                                                                    tcctcggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTCGAACTGTAATCCGAAAAGATAGACATGCTTAg                                                                        <  2:570995‑M2/72‑1 (MQ=255)                                                                      cttttcttgctgaataataattaaaattatattattaattccattgccttttgtatagtaaagttctaaATAATAATCAATTTTTAAATTATTGTTCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGcc                                                                      <  2:396599‑M2/74‑1 (MQ=255)                                                                               ctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAt                                                             >  2:430885‑M2/61‑143 (MQ=255)                                                                                tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAtt                                                            >  1:921788‑M2/60‑143 (MQ=255)                                                                                 gaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGCAAGGATAGACATGCTTAGCCACTCTCTAttt                                                           >  1:255380‑M2/59‑143 (MQ=255)                                                                                  aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGCACTGTAATCAGACAGGATAGACATGCTTAGCAAATCCAtattcg                                                          >  1:280324‑M2/58‑141 (MQ=255)                                                                                  aagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTGTATttg                                                          >  2:827389‑M2/58‑143 (MQ=255)                                                                                    gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgat                                                        >  1:344228‑M2/56‑143 (MQ=255)                                                                                       taattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgattga                                                     <  2:466748‑M2/91‑1 (MQ=255)                                                                                        aattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaa                                                    <  1:575694‑M2/92‑1 (MQ=255)                                                                                             aaatgatattattaattccactgcctttggtaaaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCAATACTGCAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGAtcgaattgaa                                               >  2:125535‑M2/47‑143 (MQ=255)                                                                                              aatgatattatttattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                              <  2:631996‑M2/98‑1 (MQ=255)                                                                                                 gatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAt                                           >  2:807622‑M2/43‑143 (MQ=255)                                                                                                     ttattaatttcactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                       <  2:1021622‑M2/105‑1 (MQ=255)                                                                                                     ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                       >  2:252989‑M2/39‑143 (MQ=255)                                                                                                     ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                       <  2:344228‑M2/105‑1 (MQ=255)                                                                                                     ttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgttt                                       <  2:921788‑M2/105‑1 (MQ=255)                                                                                                       attaattccactggctttggtagaggaaagggctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAtgtttgt                                     >  1:1051195‑M2/37‑143 (MQ=255)                                                                                                               cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                             >  2:57552‑M2/29‑143 (MQ=255)                                                                                                               cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                             <  1:1091942‑M2/115‑1 (MQ=255)                                                                                                               cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAGGTTTGTTAAGGCAt                             >  1:19937‑M2/29‑143 (MQ=255)                                                                                                                      tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCa                      >  2:832222‑M2/22‑143 (MQ=255)                                                                                                                      tttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTAGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGatgaa                      >  2:209541‑M2/22‑141 (MQ=255)                                                                                                                       ttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                     <  2:392386‑M2/123‑1 (MQ=255)                                                                                                                       ttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                     <  1:412097‑M2/123‑1 (MQ=255)                                                                                                                       ttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                     <  1:887245‑M2/123‑1 (MQ=255)                                                                                                                       gtggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCaa                     <  1:557579‑M2/123‑1 (MQ=255)                                                                                                                          gtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCt                  <  1:345274‑M2/126‑1 (MQ=255)                                                                                                                           tagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCta                 >  2:270223‑M2/17‑143 (MQ=255)                                                                                                                            agaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGCTGCAAGCtat                >  1:96777‑M2/16‑143 (MQ=255)                                                                                                                              aggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGCTGCAAGCTATAg              >  2:135961‑M2/14‑143 (MQ=255)                                                                                                                                 aaagtgttaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAtt           <  2:369608‑M2/133‑1 (MQ=255)                                                                                                                                  aagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAgatac          <  1:569454‑M2/134‑3 (MQ=255)                                                                                                                                        taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCATAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAc    >  2:152424‑M2/4‑143 (MQ=255)                                                                                                                                          aataataatCAATTGTTAAATTATTGTACATCGATCTAATAGAACTTTAATCAGAAAAGATAGACATGCTTAGCCAAACTCAATGATAAATAAATGAAAGATGTTTGGTAAGGCATGGATGCAAGCGATAGATTCTGATACgg  >  2:20227/1‑143 (MQ=255)                                                                                                                                          aATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAGGATAGACATGCTTAGCCAATCTCTATTTGATTGAATGGAAAGACGTTGCTTAAGCCGAGGAGGCAAGGTGTGAAATCTGATACgg  >  2:581386‑M2/2‑143 (MQ=255)                                                                                                                                           |                                                                                                                                              ACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGG  >  NC_000913/1300560‑1300839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑