Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499–1300697	1300697	1–1199	58 [0]	[0] 59	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	56 (1.380)	53/276	0.0	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

CGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300561‑1300840                                                                                                                                          |                                                                                                                                               cagttagttatatggatttccctaacggcttctccgcaccttcatgttattttcgccacatcataatcctgggctcgctgcaggataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataata                                                                                                                                           <  2:229375‑M2/6‑1 (MQ=255)  agttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcatgggcttgctgaagaataattgaaatgatattattaattccactacctttggtagaggaaagtgctaaataataa                                                                                                                                          >  2:45462‑M2/137‑143 (MQ=255)        ttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtataggaaagtgctaaATAATAATCAAtt                                                                                                                                    <  2:561796‑M2/13‑1 (MQ=255)          ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctacATAATAATCAATTGt                                                                                                                                  >  2:405228‑M2/129‑143 (MQ=255)           taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaggtgctaaATAATAATCAATTGtt                                                                                                                                 >  1:419597‑M2/128‑143 (MQ=255)              ggatttccttaactgcttctcctcaccatcatgttatcttcgccacatcatactcctggccttgctgaagaataattgaaatgatattattaatcccactgcctttggaataagaaagcactaaATAATCATCAATTTttagc                                                                                                                              >  2:595074‑M2/125‑141 (MQ=255)                atttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAatt                                                                                                                            >  1:460289‑M2/123‑143 (MQ=255)                 tttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAatta                                                                                                                           <  1:364700‑M2/22‑1 (MQ=255)                      ttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgtg                                                                                                                      >  1:107920‑M2/117‑143 (MQ=255)                      gtaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgataaATCATAATCAATTGTTAAATTATtgtg                                                                                                                      <  2:637856‑M2/27‑1 (MQ=255)                        aactgcttctcctcaccatcatgttattttcgccacatcataaccctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCa                                                                                                                    <  1:298019‑M2/29‑1 (MQ=255)                         actgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattcccctgcctttggtagagcaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCAt                                                                                                                   >  2:330042‑M2/114‑143 (MQ=255)                             cttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCa                                                                                                               >  2:420284‑M2/110‑143 (MQ=255)                              ttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCac                                                                                                              <  2:369944‑M2/35‑1 (MQ=255)                              ttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAAGCAATTGTTAAATTATTGTGCATTTCac                                                                                                              >  1:502682‑M2/109‑143 (MQ=255)                               tctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCact                                                                                                             >  1:3413‑M2/108‑143 (MQ=255)                                ctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccacttcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCacta                                                                                                            <  1:118457‑M2/37‑1 (MQ=255)                                   ctcaccatcatgttattttcgccacatcataatcctgggcttgctgcagaataattgaaatggtagtattagttccactgcctttggtagaggaaagtgctaaATAATAATCAATTCTTCAATTATAGTGCATTTcactactc                                                                                                         >  2:352167‑M2/104‑142 (MQ=255)                                    tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtaaagacaagcgctaaTTAATAATACATGGTCAAATTATTGTGCATTTCACTACTgg                                                                                                        >  2:117502‑M2/103‑143 (MQ=255)                                      accatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGaa                                                                                                      <  1:672772‑M2/43‑1 (MQ=255)                                            atgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTaa                                                                                                >  2:692724‑M2/95‑143 (MQ=255)                                             tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattcccctgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTATGCATTTCACTACTGGAACTGtaaa                                                                                               >  2:289892‑M2/94‑142 (MQ=255)                                              gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                              >  1:691789‑M2/93‑143 (MQ=255)                                              gttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATc                                                                                              <  2:152373‑M2/51‑1 (MQ=255)                                                tattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTCGAACTGTAATAAg                                                                                            >  2:263309‑M2/91‑143 (MQ=255)                                                 attttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTACATTTTGGTGCATTTCACTACTGGAACTGTGCTCAGa                                                                                           >  1:132631‑M2/90‑143 (MQ=255)                                                  atgtcgcaacatcataatcctgggcaagcagaaaaataattgaaatgatattataaattccactgcctttggtagaggaaagggctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaa                                                                                          <  2:474357‑M2/55‑1 (MQ=255)                                                       gccatatcataatcctgggtttgctgaataataattgacatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGAt                                                                                     <  2:2848‑M2/60‑1 (MQ=255)                                                        caacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATa                                                                                    <  2:218948‑M2/61‑1 (MQ=255)                                                         cacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctagATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAg                                                                                   <  1:317339‑M2/62‑1 (MQ=255)                                                              cataatcctgggcttgctgaagaataattgaaatgatattattaattccactgactttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATg                                                                              >  2:243762‑M2/77‑143 (MQ=255)                                                               ataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                             >  2:65476‑M2/76‑143 (MQ=255)                                                                  atcctgggcttgctgaagaataattgaaatgatattactaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTa                                                                          <  1:133487‑M2/71‑1 (MQ=255)                                                                    cctgggcttgctgaagaataattcaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGc                                                                        <  1:34592‑M2/73‑1 (MQ=255)                                                                       gggcttgctgaagaataagtgaaatgatattattaattccactgcctttggtagaggaaagtgctaaAGAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCaa                                                                     <  2:201040‑M2/76‑1 (MQ=255)                                                                               tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTatc                                                             >  1:665277‑M2/60‑142 (MQ=255)                                                                               tgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGAAATGCTTAGCCAATCTCTAtt                                                             >  1:695754‑M2/60‑143 (MQ=255)                                                                                tacgaataagtgaaatgatattattatttccacggcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAttt                                                            <  2:144049‑M2/85‑1 (MQ=255)                                                                                        attgaaatgttcttattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaat                                                    <  2:300191‑M2/93‑1 (MQ=255)                                                                                        attgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaat                                                    <  2:370295‑M2/93‑1 (MQ=255)                                                                                          tgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaattg                                                  >  1:97810‑M2/49‑143 (MQ=255)                                                                                            aaatgatattattaattccactgcttttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGAttgaattgaa                                                <  2:425836‑M2/97‑1 (MQ=255)                                                                                             aattatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                               <  2:30381‑M2/98‑1 (MQ=255)                                                                                             aatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGaaa                                               >  1:177251‑M2/46‑143 (MQ=255)                                                                                                          atgccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                                  <  1:632069‑M2/111‑1 (MQ=255)                                                                                                             ccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCa                               <  2:350870‑M2/114‑1 (MQ=255)                                                                                                               actgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATg                             >  1:218659‑M2/28‑143 (MQ=255)                                                                                                                  gcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCCGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGAt                          <  2:363593‑M2/119‑1 (MQ=255)                                                                                                                    ctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGc                        >  2:616672‑M2/23‑143 (MQ=255)                                                                                                                            gaggaaagtgctgaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTACGGCATGGATGCAAGCtata                <  1:12936‑M2/129‑1 (MQ=255)                                                                                                                             aggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAg               >  1:293210‑M2/14‑143 (MQ=255)                                                                                                                              ggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTATAATCAGAAAAGATATACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGa              <  2:47950‑M2/131‑1 (MQ=255)                                                                                                                               gaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAt             >  1:439332‑M2/12‑143 (MQ=255)                                                                                                                                 gagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACGACTGGAACTGTAATCAGAGAAGATAGACAGGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTc           <  2:143837‑M2/134‑1 (MQ=255)                                                                                                                                    tgctaaCTAATAATAAATTGTGCAATTTGTCTCCATTCCACGACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGACAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATCCTGa        <  1:366930‑M2/137‑1 (MQ=255)                                                                                                                                    tgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGa        <  1:294549‑M2/137‑1 (MQ=255)                                                                                                                                      ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGACAAGATAGACATGCTTAGCCAATCTCTATTTGATTTAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATa      <  2:198199‑M2/139‑1 (MQ=255)                                                                                                                                       taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCACGATATGACTGCTGATAc     >  2:538828‑M2/4‑143 (MQ=255)                                                                                                                                          ataataatCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAACCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGt  <  2:477770/143‑1 (MQ=255)                                                                                                                                          |                                                                                                                                               CGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATACGGT  >  NC_000913/1300561‑1300840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑