Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	96.0%	67.4 / NA	27	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (11/13);  total (13/14)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

ATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132649‑2132926                                                                                                                                           |                                                                                                                                            aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTg                                                                                                                                         >  2:531318/1‑143 (MQ=255)  tCTGGTAGAGGGTAACCAGCTGATTTTTTTTGCTGGCGCGGTCTAATTTGTCTTTGTGCTTGCGGTAAAAGTTGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                        <  2:383295/143‑1 (MQ=255)      gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATCTGACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                    >  1:131659/1‑143 (MQ=255)          agGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTGCCCGGAGAGTTTTTTCGGTGAAGAGGTGATCTGCATTTTACTGGGATTGATAGGCAGGGTATGCGTTGCctc                                                                                                                                >  1:544221/1‑143 (MQ=255)           gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctct                                                                                                                               <  1:548893/143‑1 (MQ=255)                      tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCAACGTGATTGATATGCAGGCTCTGCGTAGGCTCTTCTTCtttacc                                                                                                                    >  1:80554/1‑140 (MQ=255)                       gATATTTTTTGCTGGCGCGGTCGCATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGGGTCGCCTTTTCTACTTTCCAc                                                                                                                   >  2:623033/1‑143 (MQ=255)                            tgtgtggttgggcgGTCGATTTTGTCGTTCTGCTTGCGGCAGACGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTTATTGATATGCAGGCTCTGCGTCGCTTCTTCTACTTTTCACGGTTc                                                                                                              <  2:598335/132‑1 (MQ=255)                               ttGCTGGCGCGGTCGACGTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATCTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc                                                                                                           <  1:440633/143‑1 (MQ=255)                                  cTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTAGCATGAGAATATTTGCGGTGACGAGGTGAGCAGCAGTTCACCGTGATTCAAACACAGGATCTGCGTCGCCTCTTCTACTTTCGACGGCTCCCCGTa                                                                                                        >  2:670065/1‑143 (MQ=255)                                   tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATACAGGCTCTGCGTCGCCTCTTATCCTTTCCACGGTTAGCCGTAc                                                                                                       >  2:176722/1‑143 (MQ=255)                                          gTCGACGTTGTCTTTGTGCTTGCGGTAGAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGTTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca                                                                                                <  1:182425/143‑1 (MQ=255)                                                    tCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAg                                                                                      <  1:687978/143‑1 (MQ=255)                                                          tgCTTGCGGTAAAAGTGCAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAt                                                                                <  1:166962/143‑1 (MQ=255)                                                               gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGACGCTGCCGt                                                                           >  1:64888/1‑143 (MQ=255)                                                                                 cccGGAGACTTTTTTCGTTGACGAGGTGACCTGCATCTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                         <  2:63497/143‑1 (MQ=255)                                                                                          ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACACCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt                                                <  1:377447/143‑1 (MQ=255)                                                                                                 ggTGACGCGGTGCTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaa                                         <  1:637889/143‑1 (MQ=255)                                                                                                   tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaaccaac                                       >  1:315420/1‑143 (MQ=255)                                                                                                    gacgtcgtcaTCTGCATTTCGCCCTGATTGATATGCAGGTTTTGCGTCGCCTCTTCTACTTTCGACGGTTCGACCTACGCCACCTCCTTCCGTAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                      <  2:271551/134‑1 (MQ=255)                                                                                                    gACGGGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                      <  1:13280/143‑1 (MQ=255)                                                                                                             aTCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTCTGCCGCTTTCAGTGCGGTATCGAACCAACCCTCTTTGaa                             >  2:327456/1‑143 (MQ=255)                                                                                                               cTGCATTTCACCGTGATTGATATGCAGGCTCTCCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTCTGCCGCTTTCAGTTCCGTATCGAACAAACACTCtgtgcgac                           >  2:380983/1‑139 (MQ=255)                                                                                                                tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATGGAACAAACACTCTTTGAAACg                          >  1:665525/1‑143 (MQ=255)                                                                                                                        aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACGAACACTCTTTGAAACGCAATGccc                  >  2:94463/1‑143 (MQ=255)                                                                                                                                  atatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACACCCGGCCCCAATAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc        <  1:531318/143‑1 (MQ=255)                                                                                                                                        aGGCTCTTCGTCGCCTCTGCTGCGTTCCACGGTTCGCCGTACGCCACCGCCTTCCGCTGGAAGATGTCGTGATCCTGTTCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCATGTAAAGACCTGGtt  <  2:133786/143‑1 (MQ=255)                                                                                                                                           |                                                                                                                                            ATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132649‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑