New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2224019	66 (0.980)	3 (0.050)	3/282	10.4	4.7%	coding (850/933 nt)	pbpG	D‑alanyl‑D‑alanine endopeptidase
	?	NC_000913	2742802 =	56 (0.840)					coding (422/1227 nt)	yfiN	putative membrane‑anchored diguanylate cyclase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCG  >  NC_000913/2742660‑2742944                                                                                                                                               |                                                                                                                                               ggccGTGATAAGGAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTGTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTgg                                                                                                                                                <  1:861931/140‑1 (MQ=255) gTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTgg                                                                                                                                                >  2:186911/1‑143 (MQ=255) gTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTgg                                                                                                                                                >  1:526840/1‑143 (MQ=255)    cGTGATAAGCAGCGAGATATTCTGGCATCCTGGCATTACACCCGTACGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCC‑CCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGaa                                                                                                                                            <  1:944364/143‑1 (MQ=255)    cGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGa                                                                                                                                             >  2:1077276/1‑143 (MQ=255)         ggagcaggAGAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGTCACTGGCTCTTCCCTGCCCCCATCTTTCCATTAACTTTCTACAATAGTGAATCCATTGTCGAAGTAc                                                                                                                                        <  1:834850/141‑1 (MQ=255)          aaGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGCAACCATTGGCGAAGTACg                                                                                                                                       >  1:140509/1‑143 (MQ=255)           agcagcAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGc                                                                                                                                      <  2:343140/143‑1 (MQ=255)           agcagcAAAAGATTCTGGCATCCTAGCATTACACCCGTAAGGATCCCGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGc                                                                                                                                      <  2:825752/143‑1 (MQ=255)               gcaAAATATTCTGGCATCCTGGCATTACACCCGTACGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTCCCCCCATCATTCACCCCATTCGTCCCAATGGTGCAACCATTCGCGCAGCACGCTTaa                                                                                                                                  >  1:275029/1‑143 (MQ=255)               gcaAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTaa                                                                                                                                  <  2:682154/143‑1 (MQ=255)               gcaAAATATTCTGGCAGTCTGGCATTACACCCGTAAGGATCCAGGCGCTACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTaa                                                                                                                                  <  1:671160/143‑1 (MQ=255)                caAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGCAGTACGCTTAAc                                                                                                                                 >  2:190787/1‑143 (MQ=255)                 aaaaTACTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAAcc                                                                                                                                <  2:100427/143‑1 (MQ=255)                     tatTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCta                                                                                                                            >  2:4567/1‑142 (MQ=255)                       ttCTGGCATCCTGGCATTACACCCGTAACCCTCCAGGCGATACTTTCAGTAATTTCATAAGCAACAAACAAAACACTACCCCAATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCAcgc                                                                                                                          >  2:7591/1‑143 (MQ=255)                         cTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCACCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGa                                                                                                                        <  2:165441/143‑1 (MQ=255)                              aTCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGGCAGtt                                                                                                                   >  1:592034/1‑143 (MQ=255)                              aTCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCACTGGAGCAGTACGTTTAACCGcactcagcagtt                                                                                                                   >  1:326196/1‑132 (MQ=255)                              aTCCCGGCATTACACCCGTAAGGATCCAGGCGATACCTTCAGTAATTTCATACGCCACTGGCTTTTCCCTGCCCCCATCATTCACCCGATTCGTCAAAATGGTGAAACCATTAGCGAATTACACTTAAAAGCTAGCGACAgca                                                                                                                   >  2:526145/1‑141 (MQ=255)                                  tGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGATCGCGACAATTCAAt                                                                                                               >  1:620378/1‑143 (MQ=255)                                           acCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGAAAGGGTCAGTTCAATCAGCCAttt                                                                                                      >  1:282109/1‑143 (MQ=255)                                                   ggATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGt                                                                                              <  2:344967/143‑1 (MQ=255)                                                      tCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATCTTACCTGGtttt                                                                                           >  1:395504/1‑143 (MQ=255)                                                        cAGGCGATGCTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTtcg                                                                                         <  1:670920/143‑1 (MQ=255)                                                        cAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTtcg                                                                                         <  2:526840/143‑1 (MQ=255)                                                         aGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTtcgc                                                                                        <  1:227040/143‑1 (MQ=255)                                                              aTACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATNCAGCCGATTCGTCACAATGGTGAAACCCTTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGcc                                                                                   <  2:698357/143‑1 (MQ=255)                                                                  tttCCGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTAc                                                                               <  2:886391/143‑1 (MQ=255)                                                                  ttccAGTAATTTCCTAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTAc                                                                               <  1:658005/140‑1 (MQ=255)                                                                       gTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATATTATCAAGTTTTAGCTCGCAGTACTGaac                                                                          >  2:353561/1‑141 (MQ=255)                                                                             tCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGt                                                                    >  1:1078718/1‑143 (MQ=255)                                                                                tAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTAtt                                                                 >  2:890844/1‑143 (MQ=255)                                                                                tAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGCTCAATCAGCCATTTTATCTGGTTTTCGCCCGCCGTACTCACCGGTTGTAtt                                                                 >  1:1052087/1‑143 (MQ=255)                                                                                  aGCCACGGGCGCTGCCCGGCCCCCATCATTCAGGCGATTCGTCACTATTGTGAAACCATTGGCGAAGTACGCTGAACCGCGCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTct                                                               <  1:502689/143‑1 (MQ=255)                                                                                      aCTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGCAGTACGCTTAACCGCTCGCGACCGTTCAATCAGCCATTTTATCTGGGTTTCGCTCGCCGTACTGCCCGGTTGTATTcggctg                                                           >  2:638733/1‑143 (MQ=255)                                                                                                  cTGCCCCCATCATTCAGCCGATTCGTTACAATGGTGAAATCATTGGCTAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATc                                               <  1:7591/143‑1 (MQ=255)                                                                                                  cTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATc                                               >  1:219842/1‑143 (MQ=255)                                                                                                  cTGCCCCCATCATGCAGTCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATc                                               <  1:679441/143‑1 (MQ=255)                                                                                                   tcccccccTCATTCACCCGACTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCATCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCg                                              <  2:287944/141‑1 (MQ=255)                                                                                                        ccATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTTGCGACAGTTCCATCTGCCCTTTTATCTGGTTTTCGCTCCCTGTACTGACCGGTTGTCTTCTGCTGGCATCAGCCCTCGccctc                                         >  2:772214/1‑139 (MQ=255)                                                                                                           tcatTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTAcc                                      <  1:746624/143‑1 (MQ=255)                                                                                                            ccttCGGCGGATTCGTCACAGTGGTGAACCCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTGTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTAccc                                     <  1:363280/141‑1 (MQ=255)                                                                                                             atTCAGCGGATTCGTCACAATTGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTCTCTCGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCt                                    <  2:906594/143‑1 (MQ=255)                                                                                                             acccttggtcttCGTCACAACGGTGAAACCATTGGCGAAGTACGCTTAACCGCCCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCCGGCATCAGGCATCGCAATTACCCt                                    <  2:896271/133‑1 (MQ=255)                                                                                                               tCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCa                                  <  2:282109/143‑1 (MQ=255)                                                                                                                  gCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCAccc                               >  2:893205/1‑143 (MQ=255)                                                                                                                       gtCGTCAGTATGCGGAAACCATTGGCGAAGGTCTCTGAACCGCGCGCGACAGTTCAATCGGCCATGTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATGCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCAt                          <  1:30140/142‑1 (MQ=255)                                                                                                                                    tGACACCATTGGCGACGTACGCCTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGcc             <  2:240273/143‑1 (MQ=255)                                                                                                                                      aaaCCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTg           <  2:1078718/143‑1 (MQ=255)                                                                                                                                      aaaCCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTg           <  2:1113021/143‑1 (MQ=255)                                                                                                                                      aaaCCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTg           >  2:1074003/1‑143 (MQ=255)                                                                                                                                        aCCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGt         <  2:711333/143‑1 (MQ=255)                                                                                                                                         ccATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTa        >  2:611569/1‑143 (MQ=255)                                                                                                                                          cATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGAACCTGCAAGCGCTTTTACCCTCACCCGCCGTTTGCACAATGGCCTGGTAg       >  1:1003240/1‑143 (MQ=255)                                                                                                                                           atgggCGAAGTACGCTTAGCCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGa      <  1:1044095/140‑1 (MQ=255)                                                                                                                                            ttGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGac     >  2:1064333/1‑142 (MQ=255)                                                                                                                                              ggCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTGGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAGTGGGCTGGTAGAAgc   >  2:353942/1‑143 (MQ=255)                                                                                                                                              ggCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTGTTCGCTCGCCGTACACACCGGGTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGGAgc   >  2:165841/1‑143 (MQ=255)                                                                                                                                               gCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAgcg  >  2:641964/1‑143 (MQ=255)                                                                                                                                               |                                                                                                                                               GTACGTGATAAGCAGCAAAATATTCTGGCATCCTGGCATTACACCCGTAAGGATCCAGGCGATACTTTCAGTAATTTCATAAGCCACTGGCTCTTCCCTGCCCCCATCATTCAGCCGATTCGTCACAATGGTGAAACCATTGGCGAAGTACGCTTAACCGCTCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCG  >  NC_000913/2742660‑2742944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.