Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,105,253 T→A 5.3% L274Q (CTG→CAG)  mltC → membrane‑bound lytic murein transglycosylase C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,105,2530TA 5.3% 251.9 / 3.4 76L274Q (CTG→CAG) mltCmembrane‑bound lytic murein transglycosylase C
Reads supporting (aligned to +/- strand):  ref base T (32/40);  new base A (2/2);  total (34/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

AGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAA  >  NC_000913/3105108‑3105397
                                                                                                                                                 |                                                                                                                                                
aGCCGTTCCTATGCGCTTGGATTTATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCTCATGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTgg                                                                                                                                                <  1:231967/148‑1 (MQ=255)
aGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGTAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTgg                                                                                                                                                <  2:340827/148‑1 (MQ=255)
  ccGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGa                                                                                                                                             >  3:75954/1‑149 (MQ=255)
        cGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGa                                                                                                                                       <  7:362912/149‑1 (MQ=255)
            gcgcGGGGATTAATGCAGGGGGCACAACATACTGCCGGGACAGATGTGGTCCGCGCGCCGGGGAAATCCGGCACCCCGAGCCGCAGGTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACaa                                                                                                                                   <  6:26720/149‑1 (MQ=255)
             cgcTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAAt                                                                                                                                  <  1:261133/149‑1 (MQ=255)
              gcTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATg                                                                                                                                 <  5:192289/149‑1 (MQ=255)
              gcTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATg                                                                                                                                 <  2:31512/149‑1 (MQ=255)
              gcTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGGTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGCTGCTGAACAATg                                                                                                                                 >  6:73191/1‑149 (MQ=255)
               cTGGGATTAATGCAGGTGGTACAACATACTGCCGGTAAAGATGTGTTCCGCTCGCAGGGGAAATCGGGCACGACGAGCCGAAGTTTCTTGTTTGATCCTGCCAGAAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGt                                                                                                                                <  8:144965/149‑1 (MQ=255)
                 gggATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGttt                                                                                                                              <  6:268450/149‑1 (MQ=255)
                  ggATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGttt                                                                                                                              <  8:217477/148‑1 (MQ=255)
                  ggATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGttt                                                                                                                              <  5:254429/148‑1 (MQ=255)
                         tGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTcggc                                                                                                                      >  4:163776/1‑149 (MQ=255)
                              gtggtACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAAt                                                                                                                 >  2:113634/1‑149 (MQ=255)
                               gggtACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAAtt                                                                                                                <  4:66186/148‑1 (MQ=255)
                                 gtACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGa                                                                                                              >  1:257101/1‑149 (MQ=255)
                                  tACAACATACTGCCGGGAAAGATGTGTTACGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGa                                                                                                              >  8:181147/1‑148 (MQ=255)
                                   acaacaTACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGAt                                                                                                             >  6:115614/1‑148 (MQ=255)
                                      acaTACTGCCGGGATAGATGTGTTCCTCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCTTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAAcc                                                                                                         <  1:374803/149‑1 (MQ=255)
                                            tGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACAt                                                                                                   >  8:28439/1‑149 (MQ=255)
                                             gCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATc                                                                                                  >  2:290076/1‑149 (MQ=255)
                                             gCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATc                                                                                                  >  6:42223/1‑149 (MQ=255)
                                               cGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATcgc                                                                                                >  6:269279/1‑149 (MQ=255)
                                                 ggAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGc                                                                                                                                          <  1:89963/105‑1 (MQ=255)
                                                 ggAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGc                                                                                                                                          >  2:89963/1‑105 (MQ=255)
                                                 ggAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCgcgg                                                                                              >  4:316152/1‑149 (MQ=255)
                                                   aaaGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCgcggcg                                                                                            <  5:115614/149‑1 (MQ=255)
                                                     aGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGt                                                                                           >  1:283681/1‑148 (MQ=255)
                                                      gATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTa                                                                                         <  5:42223/149‑1 (MQ=255)
                                                            ttCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGt                                                                                   >  3:229801/1‑149 (MQ=255)
                                                                    gCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCAGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCg                                                                           >  2:313846/1‑149 (MQ=255)
                                                                     gaGGGGAAATCCGGCACTCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGCTACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTAGCTCGGCGGAATTGATAACCCAACATCGCGGCGGTATGCCGTCATCACCGc                                                                          <  7:221017/148‑1 (MQ=255)
                                                                     cAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGc                                                                          >  2:288890/1‑149 (MQ=255)
                                                                      aGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGcc                                                                         <  1:120220/149‑1 (MQ=255)
                                                                      aGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCATATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGcc                                                                         >  8:362750/1‑149 (MQ=255)
                                                                      aGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGAGCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCCACATCGCGGCGTTATGCCGTCATCACCGcc                                                                         >  5:275873/1‑149 (MQ=255)
                                                                       ggggAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATCACCCAACATCGCGGCGTTATGCCGTCATCACCGcc                                                                         >  5:243968/1‑148 (MQ=255)
                                                                       ggggAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGcc                                                                         >  5:69846/1‑148 (MQ=255)
                                                                        gggAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCta                                                                       <  1:288890/149‑1 (MQ=255)
                                                                          gAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCtat                                                                      >  5:143598/1‑148 (MQ=255)
                                                                          gAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCtat                                                                      >  7:220669/1‑148 (MQ=255)
                                                                           aaaTCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATaa                                                                    >  8:78970/1‑149 (MQ=255)
                                                                              tCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCAGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAAcgg                                                                 >  1:69482/1‑149 (MQ=255)
                                                                                cGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAAcggc                                                                <  6:143598/148‑1 (MQ=255)
                                                                                cGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAAcggc                                                                <  8:220669/148‑1 (MQ=255)
                                                                                 ggCACGCCGATCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAAcggcgg                                                              <  4:229801/149‑1 (MQ=255)
                                                                                                 gTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGtgctg                                              <  2:257101/149‑1 (MQ=255)
                                                                                                  tttCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGtgctgc                                             >  4:315183/1‑149 (MQ=255)
                                                                                                  tttCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCAGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGtgctgc                                             <  2:69482/149‑1 (MQ=255)
                                                                                                  gttCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGtgctgc                                             <  6:206892/148‑1 (MQ=255)
                                                                                                     cTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGa                                           <  7:28439/148‑1 (MQ=255)
                                                                                                     cTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGa                                           <  5:72069/148‑1 (MQ=255)
                                                                                                     cTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAg                                          >  8:91203/1‑149 (MQ=255)
                                                                                                     cTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCAGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGa                                           <  1:313846/148‑1 (MQ=255)
                                                                                                      ttGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGc                                                    <  2:350172/138‑1 (MQ=255)
                                                                                                      ttGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGc                                                    >  1:350172/1‑138 (MQ=255)
                                                                                                             aTCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACAACGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGCCAGCGTGCTGCGAGTCTTTTc                                   >  6:54120/1‑148 (MQ=255)
                                                                                                                    cAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATa                           <  2:283681/149‑1 (MQ=255)
                                                                                                                          tatTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTc                                   <  1:243681/135‑1 (MQ=255)
                                                                                                                          tatTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTc                                   >  2:243681/1‑135 (MQ=255)
                                                                                                                           atTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGc                                                    >  6:357640/1‑117 (MQ=255)
                                                                                                                           atTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGc                                                    <  5:357640/117‑1 (MQ=255)
                                                                                                                            ttGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCa                    <  1:113634/148‑1 (MQ=255)
                                                                                                                            ttGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCa                    <  3:316152/148‑1 (MQ=255)
                                                                                                                            ttGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAg                   <  1:290076/149‑1 (MQ=255)
                                                                                                                               aTACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCgca                                                          >  1:288517/1‑107 (MQ=255)
                                                                                                                               aTACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCgca                                                          <  2:288517/107‑1 (MQ=255)
                                                                                                                               aTACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCt                >  3:289406/1‑149 (MQ=255)
                                                                                                                                         cgcgTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTa                                                                                         >  8:326004/1‑66 (MQ=255)
                                                                                                                                         cgcgTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTa                                                                                         <  7:326004/66‑1 (MQ=255)
                                                                                                                                          gcgTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAAtattat     <  4:75954/149‑1 (MQ=255)
                                                                                                                                           cgTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATattatt    <  3:163776/149‑1 (MQ=255)
                                                                                                                                           cgTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATattatt    <  4:289406/149‑1 (MQ=255)
                                                                                                                                             tATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTaa  <  3:315183/149‑1 (MQ=255)
                                                                                                                                             tATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTaa  <  7:181147/149‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAACGGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATTATTAA  >  NC_000913/3105108‑3105397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: