Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 912,095 T→A 5.5% Q19L (CAA→CTA)  hcr ← HCP oxidoreductase, NADH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913912,0950TA 5.5% 233.5 / 2.6 72Q19L (CAA→CTA) hcrHCP oxidoreductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base T (32/35);  new base A (2/2);  total (34/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

TATTCACTCACGCCTGGCGTGGAGGAAATGGTGTAAGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAG  >  NC_000913/911948‑912242
                                                                                                                                                   |                                                                                                                                                   
tatTCACTCACGCCTGGCGTGGAGGAAATGGTGTAAGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCtt                                                                                                                                                     <  8:198227/148‑1 (MQ=255)
  ttCACTCACGCCTGGCGTGGAGGAAATGGTGTAAGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGc                                                                                                                                                   <  3:3855/148‑1 (MQ=255)
    cactcacGCCTGGCGTGGAGGAAATGGTGTAAGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGt                                                                                                                                                 <  4:171759/148‑1 (MQ=255)
           gCCTGGCGTGGAGGAAATGGTGTAAGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGtgat                                                                                                                                         <  3:256554/149‑1 (MQ=255)
             cTGGCGTGGAGGAAATGGTGTAAGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGtgatg                                                                                                                                        >  2:117249/1‑148 (MQ=255)
             cTGGCGTGGAGGAAATGGTGTAAGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGGTTCTTGCGTAATGtgatga                                                                                                                                       <  8:141135/149‑1 (MQ=255)
                    ggaggaAATGGTGTAAGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCa                                                                                                                                >  5:104499/1‑149 (MQ=255)
                          aaTGGTGTAAGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGc                                                                                                                           >  3:215244/1‑148 (MQ=255)
                          aaTGGTGTAAGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGc                                                                                                                           >  4:332472/1‑148 (MQ=255)
                              gtgtAAGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACgg                                                                                                                      >  7:281045/1‑149 (MQ=255)
                              gtgtAAGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACgg                                                                                                                      >  2:13786/1‑149 (MQ=255)
                              gtgtAAGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACgg                                                                                                                      <  1:288195/149‑1 (MQ=255)
                                   aGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCAtt                                                                                                                 <  3:33954/149‑1 (MQ=255)
                                    gCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCAtt                                                                                                                 >  5:55812/1‑148 (MQ=255)
                                    gCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTAGCGTAATGTGATGAACCTGCATCCGCCACGGGCAtt                                                                                                                 >  3:145899/1‑148 (MQ=255)
                                    gCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGGGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTAGCGTAATGTGATGAACCTGCATCCGCCACGGGCAtt                                                                                                                 >  3:151026/1‑148 (MQ=255)
                                       cGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGt                                                                                                                                                 >  8:83970/1‑113 (MQ=255)
                                       cGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGt                                                                                                                                                 <  7:83970/113‑1 (MQ=255)
                                        gCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTc                                                                                                            <  6:55812/149‑1 (MQ=255)
                                        gCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTc                                                                                                            <  2:34945/149‑1 (MQ=255)
                                           gCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGtt                                                                                                         <  4:283354/149‑1 (MQ=255)
                                           gCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGtt                                                                                                         <  3:321966/149‑1 (MQ=255)
                                           gCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGtt                                                                                                         >  2:20535/1‑149 (MQ=255)
                                           gCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGtt                                                                                                         <  6:116044/149‑1 (MQ=255)
                                           gCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGtt                                                                                                         <  1:47491/149‑1 (MQ=255)
                                            cGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTg                                                                                                        >  5:335202/1‑149 (MQ=255)
                                             gTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTgg                                                                                                       >  7:349523/1‑149 (MQ=255)
                                                  cGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATc                                                                                                   <  4:215244/148‑1 (MQ=255)
                                                   gCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCCGCATCCGCCACGGGCATTGAt                                                                                                              <  3:208105/136‑1 (MQ=255)
                                                   gCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCCGCATCCGCCACGGGCATTGAt                                                                                                              >  4:208105/1‑136 (MQ=255)
                                                    cTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGt                                                                                                 >  3:124491/1‑148 (MQ=255)
                                                     tGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCa                                                                                               >  3:325738/1‑149 (MQ=255)
                                                      gAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCAt                                                                                              >  1:183731/1‑149 (MQ=255)
                                                       aGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCa                                                                                               <  4:146527/147‑1 (MQ=255)
                                                       aGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATc                                                                                             <  1:117249/149‑1 (MQ=255)
                                                       aGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATc                                                                                             <  8:349523/149‑1 (MQ=255)
                                                                cGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTc                                                                                    <  2:54078/149‑1 (MQ=255)
                                                                   tGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCt                                                                                  >  4:33420/1‑148 (MQ=255)
                                                                       cAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTAGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTAcgc                                                                             <  4:145899/149‑1 (MQ=255)
                                                                       cAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTAGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTAcgc                                                                             <  4:151026/149‑1 (MQ=255)
                                                                        aGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTAcgcg                                                                            <  6:335202/149‑1 (MQ=255)
                                                                          tGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATAAGGGAAATCGTCCACACATCCGGCGTTTCTTGCTTAATGTTATGAACCTGAATCCGCCACGGTCATTGATTCTTTGGCATCGTCATCTACAAACTCCTTAcgcgat                                                                          >  2:272281/1‑147 (MQ=255)
                                                                                ttGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAg                                                                    >  7:130406/1‑149 (MQ=255)
                                                                                  gCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGt                                                                   <  8:281045/148‑1 (MQ=255)
                                                                                     cGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCt                                                               >  7:270938/1‑149 (MQ=255)
                                                                                      ggCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCtt                                                              <  1:13786/149‑1 (MQ=255)
                                                                                       gcgcGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTc                                                             <  8:270938/149‑1 (MQ=255)
                                                                                         gcgATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCAt                                                           <  7:190708/149‑1 (MQ=255)
                                                                                           gATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCtt                                                                                 >  3:45652/1‑125 (MQ=255)
                                                                                           gATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCtt                                                                                 <  4:45652/125‑1 (MQ=255)
                                                                                             tatGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGtct                                                       >  1:57482/1‑149 (MQ=255)
                                                                                                  gtagtaATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTcttctt                                                   >  7:139343/1‑148 (MQ=255)
                                                                                                  gtagtaATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCttcttc                                                  <  3:104354/149‑1 (MQ=255)
                                                                                                   tagtaATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCttcttc                                                  >  2:322822/1‑148 (MQ=255)
                                                                                                   tagtaATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCttcttc                                                  >  3:282799/1‑148 (MQ=255)
                                                                                                          cGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAgtgg                                          >  7:11635/1‑149 (MQ=255)
                                                                                                              gCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGAt                                      <  2:183731/149‑1 (MQ=255)
                                                                                                                   tCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCACCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAAc                                 >  7:110919/1‑149 (MQ=255)
                                                                                                                      gtgAAATCGACCACACATCCGGCGTTTCTTGCGTAATGTTATGAACCTGCATCCTCCAAGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCa                              <  2:102592/147‑1 (MQ=255)
                                                                                                                      gggAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTTATGAACCTGCATCCGCCAATGGCATTGATTCGTTGTCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCa                              <  1:272281/149‑1 (MQ=255)
                                                                                                                      gggAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCa                              <  1:322822/149‑1 (MQ=255)
                                                                                                                        gAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAgtgg                                          <  2:358062/135‑1 (MQ=255)
                                                                                                                        gAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAgtgg                                          >  1:358062/1‑135 (MQ=255)
                                                                                                                         aaaTCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGc                           >  1:129336/1‑149 (MQ=255)
                                                                                                                                    acaTCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCg                <  8:11635/149‑1 (MQ=255)
                                                                                                                                       tCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTc             <  3:332472/149‑1 (MQ=255)
                                                                                                                                         cGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCa            <  4:325738/148‑1 (MQ=255)
                                                                                                                                              tttCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCg      >  7:298605/1‑149 (MQ=255)
                                                                                                                                              tttCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCg      >  7:85005/1‑149 (MQ=255)
                                                                                                                                                tCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGc     >  5:278855/1‑148 (MQ=255)
                                                                                                                                                tCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGc     <  4:124491/148‑1 (MQ=255)
                                                                                                                                                tCTGGCGTAATGGGATGAACCTGCATCCGCCACGGGCATTGATTCGGTGGCATCGTCATCGACAAACTCCTTCCGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGc     <  2:294164/148‑1 (MQ=255)
                                                                                                                                                 cTTGCGTAATGTGATGAACCTGCATCAGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTAAACAGTTGCTTCATGTCTTATTCAACAGTGGTTATAGAACGAATGCCGAATTTCTCGTTCAGCACCGc     >  6:145295/1‑147 (MQ=255)
                                                                                                                                                  ttGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCcag  >  5:290149/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TATTCACTCACGCCTGGCGTGGAGGAAATGGTGTAAGCACGCAGCGTTTCCGCTGAGTTTCGCACGCTGACCAGTGCATATTGCCCGGCGCGATATGGGTAGTAATCGTGGCAAATCAGGGAAATCGTCCACACATCCGGCGTTTCTTGCGTAATGTGATGAACCTGCATCCGCCACGGGCATTGATTCGTTGGCATCGTCATCGACAAACTCCTTACGCGCTCAACAGTTGCTTCATGTCTTCTTCAACAGTGGTGATAGAACGCAGGCCGAATTTCTCGTTCAGCACCGCCAG  >  NC_000913/911948‑912242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑