Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,806,971 A→C 18.9% Q201P (CAG→CCG)  pfkB → 6‑phosphofructokinase II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,806,9710AC18.9% 164.5 / 35.5 85Q201P (CAG→CCG) pfkB6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base A (33/36);  new base C (7/9);  total (40/45)
Fisher's exact test for biased strand distribution p-value = 7.89e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

ATTTCCGCTGCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGA  >  NC_000913/1806826‑1807117
                                                                                                                                                 |                                                                                                                                                   
aTTTCCGCTGCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCCGcc                                                                                                                                                  >  3:245181/1‑149 (MQ=255)
       cTGCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGAt                                                                                                                                           <  8:181405/149‑1 (MQ=255)
           gcAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTcc                                                                                                                                       >  3:43874/1‑149 (MQ=255)
           gcAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTcc                                                                                                                                       <  4:88403/149‑1 (MQ=255)
            cAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGGTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCg                                                                                                                                      <  4:199240/149‑1 (MQ=255)
                 aCAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAg                                                                                                                                 >  4:167129/1‑149 (MQ=255)
                   aaGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGcc                                                                                                                               >  6:31872/1‑149 (MQ=255)
                        aTCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCa                                                                                                                          <  8:33803/149‑1 (MQ=255)
                                cATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCGGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAAt                                                                                                                    <  2:110143/147‑1 (MQ=255)
                                cATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAAt                                                                                                                    <  2:104423/147‑1 (MQ=255)
                                cATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCg                                                                                                                  <  1:76878/149‑1 (MQ=255)
                                 aTCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGt                                                                                                                 <  4:316150/149‑1 (MQ=255)
                                 aTCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGAGAACTCACCCAGCCGGACTATGTCCGCAAAGCCGCGCAGGAAATCGt                                                                                                                 <  7:65341/149‑1 (MQ=255)
                                    gtcgACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTaa                                                                                                              <  4:121728/149‑1 (MQ=255)
                                     tcgACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAAt                                                                                                             <  7:88199/149‑1 (MQ=255)
                                       gACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTACGCCTAACCAAAAAGAACTCTGTGCGCTGGTGAATCGCGAACTCAGCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATa                                                                                                            <  5:314727/148‑1 (MQ=255)
                                       gACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATa                                                                                                            <  7:324367/148‑1 (MQ=255)
                                         cAGTTCTGGCGAAGCGGTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCg                                                                                                         <  2:273539/149‑1 (MQ=255)
                                           gTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGc                                                                                                       <  5:204722/149‑1 (MQ=255)
                                           gTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGc                                                                                                       <  5:46465/149‑1 (MQ=255)
                                                 gCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCTAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCCGCCGGTCGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGc                                                                                                  >  4:142418/1‑148 (MQ=255)
                                                 gCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAgg                                                                                                   <  2:78054/147‑1 (MQ=255)
                                                    aaGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCaaa                                                                                              >  7:345947/1‑149 (MQ=255)
                                                    aaGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCaaa                                                                                              <  4:43874/149‑1 (MQ=255)
                                                     aGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTCGTTAAGCCTAACCAAAAAGAACTCAGAGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGACCGC‑AAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCaaa                                                                                              <  1:344301/147‑1 (MQ=255)
                                                       cGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACgg                                                                                           <  5:70370/149‑1 (MQ=255)
                                                        gTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACggg                                                                                          <  6:273394/149‑1 (MQ=255)
                                                             gTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTc                                                                                     <  4:245181/149‑1 (MQ=255)
                                                             gTGCAGCACTGGCAATGGGTACCATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTc                                                                                     <  1:59929/149‑1 (MQ=255)
                                                              tGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGGGAATCGCGAACTCACCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCg                                                                                    <  8:115193/149‑1 (MQ=255)
                                                                     cTGGCTATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGTTGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTccca                                                                             <  2:80259/149‑2 (MQ=255)
                                                                     cTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGt                                                                                                                 >  7:299928/1‑113 (MQ=255)
                                                                     cTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGt                                                                                                                 <  8:299928/113‑1 (MQ=255)
                                                                     cTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTcc                                                                               >  5:236041/1‑147 (MQ=255)
                                                                     cTGGCAATTGGTAACATCGAGTTGGTTAAGACTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCTCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTcc                                                                               >  4:61305/1‑147 (MQ=255)
                                                                      tGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTTAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGTGTTGTCGTTTcccag                                                                            >  5:7167/1‑147 (MQ=255)
                                                                          aaTTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGT‑c                                                                       >  7:228528/1‑149 (MQ=255)
                                                                          aaTTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑c                                                                       >  7:53600/1‑149 (MQ=255)
                                                                               gTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCATTGCGCTTGTGAATCGCGAACTCACCCAGCCGCACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCTTTTCCCAGGGT‑CCACaa                                                                  <  4:13221/149‑1 (MQ=255)
                                                                               gTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACaa                                                                  <  8:345947/149‑1 (MQ=255)
                                                                               gTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATAGCGAACTCACCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCGTGGT‑ccca                                                                    >  7:273902/1‑145 (MQ=255)
                                                                                  aCATCGAGTTGGTTAAGCCTAACCAATAAGATCTCAGTGGGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGTAAATCTTTAATAGCGGCTATGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACATgg                                                                <  4:175475/148‑1 (MQ=255)
                                                                                  aCATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAg                                                                 >  1:90075/1‑147 (MQ=255)
                                                                                  aCATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAg                                                                 >  3:309895/1‑147 (MQ=255)
                                                                                    aTCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAgc                                                             >  2:269499/1‑149 (MQ=255)
                                                                                       gAGTTGGATAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGCCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTGTCCCAGGGT‑CCACAAGGAGCGCt                                                          <  8:309972/149‑1 (MQ=255)
                                                                                        aGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCCCCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGT‑CCACAAGGAGCGCTg                                                         >  2:295540/1‑149 (MQ=255)
                                                                                         gTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTgg                                                        <  5:31872/149‑1 (MQ=255)
                                                                                            ggTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGCTGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAACCGGGTTGTCGTTTCCCTGGGT‑CCACAAGGAGCGCTggggg                                                     >  2:46010/1‑147 (MQ=255)
                                                                                            ggTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGT‑CCACAAGGAGCGCTGGgtg                                                     >  4:250882/1‑149 (MQ=255)
                                                                                             gTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGgtgt                                                    >  8:61607/1‑149 (MQ=255)
                                                                                              ttAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGT‑CCACAAGGAGCGCTGGGTGtt                                                   >  6:257683/1‑149 (MQ=255)
                                                                                              ttAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCTCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGtt                                                   >  5:97836/1‑149 (MQ=255)
                                                                                              ttAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGtt                                                   >  5:78190/1‑149 (MQ=255)
                                                                                              ttAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGtt                                                   >  7:138699/1‑149 (MQ=255)
                                                                                                aaGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTg                                                  <  8:53600/148‑1 (MQ=255)
                                                                                                 aGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTGa                                                 <  7:61607/148‑1 (MQ=255)
                                                                                                  gCCTAACCAAAAAGAACTCAGGGCGCTGGGTAATCGCGAACTCACCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTAGTTTCCCTGGGT‑ACACAAGGAGCGCTGGGTGTTGATa                                               <  3:12729/149‑1 (MQ=255)
                                                                                                     tAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTccca                                                                             <  1:84060/117‑2 (MQ=255)
                                                                                                     tAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTccca                                                                             >  2:84060/1‑116 (MQ=255)
                                                                                                     tAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTCGGTGTTGATAGt                                             >  1:176157/1‑148 (MQ=255)
                                                                                                      aaCCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTg                                            >  7:327517/1‑148 (MQ=255)
                                                                                                         cataaaGAACTCAGTGAGCTGGTGAATCGCGAACTCACCCCTCCGGACGATGTCCGCAAATCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAATCGGGTTGTCGTTTCCCTGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                        <  3:230627/146‑1 (MQ=255)
                                                                                                         cAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                        >  7:262756/1‑149 (MQ=255)
                                                                                                         cAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGAAGGAAAACGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                        >  2:23003/1‑149 (MQ=255)
                                                                                                          aaaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGAAAAc                                       >  7:57000/1‑149 (MQ=255)
                                                                                                          aaaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGAAAAc                                       >  1:266271/1‑149 (MQ=255)
                                                                                                           acaaGACCTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCTACCGCGTGGGCGTGTCCCAGGGTACCAC‑AGCAGCGCGGGGGGTTGATAGTGatcact                                      >  8:239513/3‑144 (MQ=255)
                                                                                                           aaaaGAACTCAGTGCGCTGGTGAATCGCGAACTCCCCCAGCCGGACGATGTCCGCAAAGCCGCGAAGGAAAACGTTAATAGCGTCAAGGCCAAACGGGTTGTCGTTGCCCAGGGT‑CCACACGGAGCGCTGGGTGTTGAAATTGaaaaat                                      >  8:239514/1‑147 (MQ=255)
                                                                                                             aaGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGt                                    <  4:309895/149‑1 (MQ=255)
                                                                                                                   tCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGT‑CCACAAGGAGCGCTGGGTGTTGa                                                 >  6:163216/1‑130 (MQ=255)
                                                                                                                   tCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGT‑CCACAAGGAGCGCTGGGTGTTGa                                                 <  5:163216/130‑1 (MQ=255)
                                                                                                                            tGGTGAATCGCGAACTCACCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGT‑CCCCAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGcca                     >  7:166956/1‑149 (MQ=255)
                                                                                                                                 aaTCGCGAACTCACCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGGCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCcaccac                 <  3:142418/148‑1 (MQ=255)
                                                                                                                                 aaTCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCaccacc                >  5:237023/1‑149 (MQ=255)
                                                                                                                                 aaTCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAACGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGGTGATAGTGAAAACTGTATTCAGGTGGTGCCaccacc                <  1:323891/149‑1 (MQ=255)
                                                                                                                                   tCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCgg              <  6:97836/149‑1 (MQ=255)
                                                                                                                                      cgAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGTCCAAACCGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGCTGCCACCACCGGTg            >  4:296480/1‑148 (MQ=255)
                                                                                                                                         aCTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGaaaa        <  6:128120/149‑1 (MQ=255)
                                                                                                                                           tCACCCCGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGc      >  4:196370/1‑149 (MQ=255)
                                                                                                                                           tCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGc      >  7:175413/1‑149 (MQ=255)
                                                                                                                                               ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCaga  <  2:266271/149‑1 (MQ=255)
                                                                                                                                               ccAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCAGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCaga  <  6:236041/149‑1 (MQ=255)
                                                                                                                                                cAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                        <  6:250392/110‑1 (MQ=255)
                                                                                                                                                cAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGaaaa                                        >  5:250392/1‑110 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
ATTTCCGCTGCGCAAAAACAAGGGATCCGCTGCATCGTCGACAGTTCTGGCGAAGCGTTAAGTGCAGCACTGGCAATTGGTAACATCGAGTTGGTTAAGCCTAACCAAAAAGAACTCAGTGCGCTGGTGAATCGCGAACTCACCCAGCCGGACGATGTCCGCAAAGCCGCGCAGGAAATCGTTAATAGCGGCAAGGCCAAACGGGTTGTCGTTTCCCTGGGT‑CCACAAGGAGCGCTGGGTGTTGATAGTGAAAACTGTATTCAGGTGGTGCCACCACCGGTGAAAAGCCAGA  >  NC_000913/1806826‑1807117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑