Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,635,061 C→T 5.8% T277I (ACC→ATC)  xseA → exonuclease VII, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,635,0610CT 5.8% 279.7 / 4.8 86T277I (ACC→ATC) xseAexonuclease VII, large subunit
Reads supporting (aligned to +/- strand):  ref base C (46/35);  new base T (2/3);  total (48/38)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

AGCCGCATTCCGGTTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCATGCTTGAGCGCCTGCAAAAGCGAA  >  NC_000913/2634913‑2635207
                                                                                                                                                    |                                                                                                                                                  
aGCCGCATTCCGGTTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGAc                                                                                                                                                    <  1:124570/149‑1 (MQ=255)
     cATTCCGGTTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGt                                                                                                                                                >  2:354322/1‑148 (MQ=255)
       ttCCGGTTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCaa                                                                                                                                             <  6:130505/149‑1 (MQ=255)
          cGGTTGTCAGCGCCGTCGGGCATGAGACGGATGTTACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATAAGAAAGAGTTACTGCGCCAGGTGCAATCGACACGTAAACgg                                                                                                                                          >  4:94974/1‑149 (MQ=255)
          cGGTTGGCAGCGCCGTCGGGCATGAGACGGATGTGACCTTTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACg                                                                                                                                           <  5:144545/148‑1 (MQ=255)
            gTTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTATGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCt                                                                                                                                        >  1:70628/1‑149 (MQ=255)
             ttGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTg                                                                                                                                       <  7:216100/149‑1 (MQ=255)
                 cAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTg                                                                                                                                       >  3:240571/1‑145 (MQ=255)
                  aGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGgaga                                                                                                                                   <  8:247636/148‑1 (MQ=255)
                   gcgcCGTCGGGCATGAGACGGATGTGACCATTGCAGATTTTGTTGCCTATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATg                                                                                                                                 <  2:59972/149‑1 (MQ=255)
                    cgcCGTCGGGCATGAGACGGATGGGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATgg                                                                                                                                <  8:18340/149‑1 (MQ=255)
                      ccGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGc                                                                                                                               >  1:21233/1‑148 (MQ=255)
                               aTGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGattat                                                                                                                     >  8:7601/1‑149 (MQ=255)
                                tGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGattat                                                                                                                     >  2:140178/1‑148 (MQ=255)
                                 gagaCGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGAttatta                                                                                                                   >  2:299870/1‑149 (MQ=255)
                                       gATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGc                                                                                                             >  5:296885/1‑149 (MQ=255)
                                         tgtgACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGc                                                                                                                                         >  6:155719/1‑119 (MQ=255)
                                         tgtgACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGc                                                                                                                                         <  5:155719/119‑1 (MQ=255)
                                               cATTTCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGc                                                                                                      >  1:223291/1‑148 (MQ=255)
                                               cATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGca                                                                                                     <  2:84420/149‑1 (MQ=255)
                                                    ccGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAAGCGACCCGTCAACGGCTGGAGATGGCGATGGGTTATTATCTCGCCCACCGCACACGt                                                                                                >  2:243029/1‑149 (MQ=255)
                                                      gagtttGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTACGCCAGGTGCAATCGACCCGTCAAAGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCg                                                                                              >  4:52870/4‑149 (MQ=255)
                                                      gATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCCACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCg                                                                                              <  7:249783/149‑1 (MQ=255)
                                                         tttGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGATCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCtt                                                                                           >  8:44836/1‑149 (MQ=255)
                                                         tttGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGATCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCtt                                                                                           >  8:50098/1‑149 (MQ=255)
                                                            gttgCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGATCCGTCAACGTCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTa                                                                                         <  1:144753/148‑1 (MQ=255)
                                                             ttgCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCCCACGTCGCTTTACg                                                                                       >  1:302455/1‑149 (MQ=255)
                                                             ttgCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTAc                                                                                        <  1:168191/148‑1 (MQ=255)
                                                             ttgCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTAc                                                                                        <  1:47396/148‑1 (MQ=255)
                                                             ttgCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACg                                                                                       >  3:317044/1‑149 (MQ=255)
                                                               gCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTCAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGc                                                                                      >  8:112147/1‑148 (MQ=255)
                                                                ccGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCa                                                                                     >  3:232848/1‑148 (MQ=255)
                                                                  gagcTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGAGGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAAt                                                                                  >  4:312456/4‑149 (MQ=255)
                                                                    gcggcgtgcgCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTCCTGCGCCAGGGGCAATCGACCCGTCAACGGCTGGAGAGGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTCCGCAAATtc                                                                                <  6:304392/146‑1 (MQ=255)
                                                                        cgtgcgCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAAt                                                                                  <  1:140178/143‑1 (MQ=255)
                                                                        cgtgcgCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATtcatca                                                                            <  8:74706/149‑1 (MQ=255)
                                                                        cgtgcgCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATtcat                                                                              <  1:354322/147‑1 (MQ=255)
                                                                                 aCGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGca                                                                                                     <  1:237817/115‑1 (MQ=255)
                                                                                 aCGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGca                                                                                                     >  2:237817/1‑115 (MQ=255)
                                                                                   gCCGTCTGCCGCCGCTGAAGTCGGGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTacagc                                                                 <  4:45428/149‑1 (MQ=255)
                                                                                     cGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTacagcaa                                                               >  1:186155/1‑149 (MQ=255)
                                                                                     cGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTacagcaa                                                               <  1:299870/149‑1 (MQ=255)
                                                                                     cGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTacagcaa                                                               <  2:223291/149‑1 (MQ=255)
                                                                                           ccgccgCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCAt                                                         >  6:201091/1‑149 (MQ=255)
                                                                                            cgccgcTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCACCGGCTGGAGATGGCGATGGATTATTATCTCGCCCACCGCACACGTCGCGTTACGCAAATTCATCACCGATTACAGCAACAGCATc                                                        >  5:340656/1‑149 (MQ=255)
                                                                                             gccgcTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATcc                                                       <  4:111678/149‑1 (MQ=255)
                                                                                                       tagtGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCg                                                                         <  1:14514/121‑1 (MQ=255)
                                                                                                       tagtGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCg                                                                         >  2:14514/1‑121 (MQ=255)
                                                                                                       tagtGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCgg                                             <  4:240571/149‑1 (MQ=255)
                                                                                                         gtGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTg                                                                                                                                       <  8:145718/57‑1 (MQ=255)
                                                                                                         gtGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTg                                                                                                                                       >  7:145718/1‑57 (MQ=255)
                                                                                                         gtGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGc                                            <  2:24774/148‑1 (MQ=255)
                                                                                                         gtGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGc                                            <  4:232848/148‑1 (MQ=255)
                                                                                                         gtGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCt                                           <  7:519/149‑1 (MQ=255)
                                                                                                          tGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTg                                          >  2:146171/1‑149 (MQ=255)
                                                                                                          tGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTg                                          >  6:186516/1‑149 (MQ=255)
                                                                                                           gAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTgg                                         >  2:19214/1‑149 (MQ=255)
                                                                                                            aGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTgg                                         <  5:186516/148‑1 (MQ=255)
                                                                                                             gCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGATCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCa                                       <  7:50098/149‑1 (MQ=255)
                                                                                                             gCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGATCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCa                                       <  7:44836/149‑1 (MQ=255)
                                                                                                             gCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGAAGCTCCGGCTGGCa                                       >  5:314207/1‑149 (MQ=255)
                                                                                                                gTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGc                                    <  2:70628/149‑1 (MQ=255)
                                                                                                                 tAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGc                                    >  4:237327/1‑148 (MQ=255)
                                                                                                                 tAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGc                                    >  2:54169/1‑148 (MQ=255)
                                                                                                                   aTCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCa                                  <  7:7601/148‑1 (MQ=255)
                                                                                                                     cAGCAAGAGTTACTGCGCCAGGTGCAATCGCCCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCCCGCCAGc                                >  2:114974/1‑148 (MQ=255)
                                                                                                                      aGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCa                               >  7:76695/1‑148 (MQ=255)
                                                                                                                        cAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAAc                            >  8:209833/1‑149 (MQ=255)
                                                                                                                                aCTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCaa                              <  3:65387/139‑1 (MQ=255)
                                                                                                                                aCTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCaa                              >  4:65387/1‑139 (MQ=255)
                                                                                                                                aCTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCATGCtt                     <  2:21233/148‑1 (MQ=255)
                                                                                                                                aCTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGAATACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCATGCtt                     >  5:9523/1‑148 (MQ=255)
                                                                                                                                 cTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCATGCtt                     >  3:261825/1‑147 (MQ=255)
                                                                                                                                    cgcCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCCTGCTTGAgcg                >  5:51368/1‑149 (MQ=255)
                                                                                                                                    cgcCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCATGCTTGAgc                 <  2:186155/148‑1 (MQ=255)
                                                                                                                                    cgcCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTAGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGCTTACAGCAACAGCAGCCGCAGCTCCGGCTGGCACTCCAGCAAACCATGCTTGAgc                 <  3:94974/148‑1 (MQ=255)
                                                                                                                                      ccAGGTGCAATCGCCCCGTCAACGGCTGGAGATGGCGAGGGATTATTGTCTCGCCAACCGCACCCGTCGCTTTACGCAAATTCCTGCCCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCCCGCCCGCAAACCATGCTTGAGCGcc              >  2:193865/1‑149 (MQ=255)
                                                                                                                                      ccAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGc                                            >  6:255596/1‑119 (MQ=255)
                                                                                                                                      ccAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCATGCTTGAGCGcc              <  3:312456/149‑1 (MQ=255)
                                                                                                                                       cAGGTGCAATCGCCCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACCGCACCAGCATACGCAGCTCCGGCTGGCCCGCCAGCAAACCATGCTTGAGCGCCt             >  8:240651/1‑149 (MQ=255)
                                                                                                                                       cAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCATGCTTGAGCGCCt             >  8:243964/1‑149 (MQ=255)
                                                                                                                                       cAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCATGCTTGAGCGCCt             <  3:237327/149‑1 (MQ=255)
                                                                                                                                        aGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGGTGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCATGCTTGAGCGCCTg            >  2:70499/1‑149 (MQ=255)
                                                                                                                                          gTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCCTGCTTGAGCGCCTGc           >  8:7545/1‑148 (MQ=255)
                                                                                                                                            gCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCATGCTTGAGCGCCTGCaa         >  6:324072/1‑148 (MQ=255)
                                                                                                                                                tCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCATGCTTGAGCGCCTGCAAAAGc     >  3:79653/1‑148 (MQ=255)
                                                                                                                                                  gACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCATGCTTGAGCGCCTGCAAAAGCGaa  >  8:153156/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AGCCGCATTCCGGTTGTCAGCGCCGTCGGGCATGAGACGGATGTGACCATTGCCGATTTTGTTGCCGATCTGCGTGCGCCAACGCCGTCTGCCGCCGCTGAAGTAGTGAGCCGTAATCAGCAAGAGTTACTGCGCCAGGTGCAATCGACCCGTCAACGGCTGGAGATGGCGATGGATTATTATCTCGCCAACCGCACACGTCGCTTTACGCAAATTCATCACCGATTACAGCAACAGCATCCGCAGCTCCGGCTGGCACGCCAGCAAACCATGCTTGAGCGCCTGCAAAAGCGAA  >  NC_000913/2634913‑2635207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: