Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,615,076	T→A	F91L (TTT→TTA)	yneJ →	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,615,076	0	T	A	100.0%	313.7 / NA	88	F91L (TTT→TTA)	yneJ	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (56/32);  total (56/32)

GGTTGATCTGTTTATTCGCGAGAATCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTTTCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTGGAGGGAAAACT  >  NC_000913/1614935‑1615219                                                                                                                                              |                                                                                                                                                ggTTGATCTGTTTATTCGCGAGAATCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGACGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTg                                                                                                                                          <  1:163049/149‑1 (MQ=255) ggTTGATCTGTTTATTCGCGAGAATCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTg                                                                                                                                          <  4:109505/149‑1 (MQ=255)  gTTGATCTGTTTATTCGCGAGAATCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTgg                                                                                                                                         >  4:246655/1‑149 (MQ=255)          gTTTATTCGCGAGAATCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTg                                                                                                                                  >  1:207019/1‑148 (MQ=255)           tttATTCGCGAGAATCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGGTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCt                                                                                                                                   <  8:375256/146‑1 (MQ=255)                tCGCGAGAATCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAg                                                                                                                             <  3:36059/147‑1 (MQ=255)                tCGCGAGAATCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAg                                                                                                                             >  4:36059/1‑147 (MQ=255)                  gcgAGAATCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCAcc                                                                                                                         >  2:269928/1‑149 (MQ=255)                  gcgAGAATCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCAcc                                                                                                                         >  1:435672/1‑149 (MQ=255)                  gcgAGAATCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCAc                                                                                                                          <  5:254161/148‑1 (MQ=255)                   cgAGAATCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCg                                                                                                                        >  8:290627/1‑149 (MQ=255)                    gagaATCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGc                                                                                                                       >  7:444816/1‑149 (MQ=255)                         tCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAg                                                                                                                  >  5:112834/1‑149 (MQ=255)                         tCAGCCTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACAGCTCCAg                                                                                                                  >  7:544877/1‑149 (MQ=255)                          cAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGt                                                                                                                 >  3:293030/1‑149 (MQ=255)                           aGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTg                                                                                                                >  7:232955/1‑149 (MQ=255)                            gCGTTTACGTCTCTCTCCTGCCGTGCATAACTTTTTACGCTATATCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTTGAAAGCACCGCTGCAGTgc                                                                                                               <  4:405961/149‑1 (MQ=255)                            gCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCAc                                                                                                                          <  6:428824/138‑1 (MQ=255)                            gCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCAc                                                                                                                          >  5:428824/1‑138 (MQ=255)                                ttACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCAcc                                                                                                                         >  7:32470/1‑135 (MQ=255)                                ttACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCAcc                                                                                                                         <  8:32470/135‑1 (MQ=255)                                 tACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCAt                                                                                                           <  8:137510/148‑1 (MQ=255)                                  aCGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTc                                                                                                         >  5:135957/1‑149 (MQ=255)                                    gTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCa                                                                                                       <  3:246655/149‑1 (MQ=255)                                     tctctctcCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAg                                                                                                      >  1:204858/1‑149 (MQ=255)                                      ctctctcCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGc                                                                                                     >  7:220836/1‑149 (MQ=255)                                       tctctcCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGcc                                                                                                    >  4:200003/1‑149 (MQ=255)                                         tctcCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCAc                                                                                                  >  8:162894/1‑149 (MQ=255)                                           tcCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGc                                                                                                >  6:339138/1‑149 (MQ=255)                                           tcCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGc                                                                                                >  3:438426/1‑149 (MQ=255)                                           tcCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGc                                                                                                >  3:464309/1‑149 (MQ=255)                                           tcCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGc                                                                                                >  4:470327/1‑149 (MQ=255)                                              tGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTgg                                                                                             >  5:233816/1‑149 (MQ=255)                                               gCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGc                                                                                            >  5:64252/1‑149 (MQ=255)                                                    gCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAAt                                                                                       <  7:290627/149‑1 (MQ=255)                                                      aTAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAAtt                                                                                      >  6:393794/1‑148 (MQ=255)                                                          cTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAAcc                                                                                 <  7:162894/149‑1 (MQ=255)                                                          cTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAAcc                                                                                 >  6:399033/1‑149 (MQ=255)                                                           tttttACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACcg                                                                                >  1:536318/1‑149 (MQ=255)                                                           tttttACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACcg                                                                                >  7:500281/1‑149 (MQ=255)                                                           tttttACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACcg                                                                                >  8:332758/1‑149 (MQ=255)                                                                    tataGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATcc                                                                       >  1:298661/1‑149 (MQ=255)                                                                         ccAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCaaaa                                                                  <  1:524367/149‑1 (MQ=255)                                                                         ccAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCaaaa                                                                  <  1:269928/149‑1 (MQ=255)                                                                         ccAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCaaaa                                                                  >  6:234788/1‑149 (MQ=255)                                                                          caacaaATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAAt                                                                 >  1:549391/1‑149 (MQ=255)                                                                          caacaaATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAAt                                                                 >  1:78453/1‑149 (MQ=255)                                                                             caaATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAAtt                                                                >  7:33581/1‑147 (MQ=255)                                                                                 ttCTCGCGTTATTGGATGAAGAGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAATCACCTCTGCAGTGCTCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGttt                                                          <  3:31022/149‑1 (MQ=255)                                                                                    tcGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCa                                                       >  5:406549/1‑149 (MQ=255)                                                                                        gTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACttt                                                   >  8:43852/1‑149 (MQ=255)                                                                                            gTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCa                                                <  6:111023/148‑1 (MQ=255)                                                                                            gTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCa                                                <  2:536318/148‑1 (MQ=255)                                                                                            gTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCAc                                               >  2:224754/1‑149 (MQ=255)                                                                                               gATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATc                                                                        <  8:226507/121‑1 (MQ=255)                                                                                               gATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATc                                                                        >  7:226507/1‑121 (MQ=255)                                                                                               gATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCgg                                            >  6:306187/1‑149 (MQ=255)                                                                                                aTGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGc                                           <  4:293030/149‑1 (MQ=255)                                                                                                   aaGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCt                                        >  4:349926/1‑149 (MQ=255)                                                                                                    aGCGCGGAGCGTAGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCTAcaaaaact                                        >  8:431322/1‑141 (MQ=255)                                                                                                     gcgcGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTc                                      <  3:200003/149‑1 (MQ=255)                                                                                                     gcgcGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTc                                      >  6:81291/1‑149 (MQ=255)                                                                                                      cgcgGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATctct                                                                                                                                            <  5:281170/46‑1 (MQ=255)                                                                                                      cgcgGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATctct                                                                                                                                            >  6:281170/1‑46 (MQ=255)                                                                                                      cgcgGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTcc                                     <  2:204858/149‑1 (MQ=255)                                                                                                       gcgGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCAGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCg                                    <  8:220836/149‑1 (MQ=255)                                                                                                        cgGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCgg                                   >  7:312716/1‑149 (MQ=255)                                                                                                              gTTGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTAt                             <  1:224754/149‑1 (MQ=255)                                                                                                               ttGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATg                            >  7:110970/1‑149 (MQ=255)                                                                                                               ttGTCGCTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTAAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATg                            >  2:43509/1‑149 (MQ=255)                                                                                                                     cTGGCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGAt                      <  8:33581/149‑1 (MQ=255)                                                                                                                        gCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATg                     <  8:444816/147‑1 (MQ=255)                                                                                                                        gCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGgt                   <  5:81291/149‑1 (MQ=255)                                                                                                                        gCGATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGgt                   <  1:43509/149‑1 (MQ=255)                                                                                                                          gATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAAGCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGgtg                  >  6:88590/1‑148 (MQ=255)                                                                                                                          gATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGgtg                  >  3:410409/1‑148 (MQ=255)                                                                                                                          gATGAGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGgtg                  >  2:440488/1‑148 (MQ=255)                                                                                                                              aGCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTg             >  3:361060/1‑149 (MQ=255)                                                                                                                               gCCGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTg             >  3:116574/1‑148 (MQ=255)                                                                                                                                ccGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTGGa           <  5:399033/149‑1 (MQ=255)                                                                                                                                ccGCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTACACTATCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTgg            <  6:233816/148‑1 (MQ=255)                                                                                                                                  gCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTGGAgg         >  4:2237/1‑149 (MQ=255)                                                                                                                                  gCAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTGGAgg         >  6:502151/1‑149 (MQ=255)                                                                                                                                   cAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTGGAggg        <  5:306187/149‑1 (MQ=255)                                                                                                                                   cAAGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTGGAgg         >  3:103654/1‑148 (MQ=255)                                                                                                                                    aaGGTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTGGAggg        >  7:243499/1‑148 (MQ=255)                                                                                                                                     tggTTTATTATGTCTTGGTTCGCTGGATAGCTCCGGTGCTGTGCGCAGTCCAGCCACGCTGGCGGATTTCAAGCGTCGTTATCCCATATTTCAGTTTTCACTTTGCTCCGGCCCTTCCGGCACTATGCTGGATGGTGTACTGGAGGGaa      <  2:435672/148‑1 (MQ=255)                                                                                                                                       gTTTATTATCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTGGAGGGaaaa    >  1:276332/1‑149 (MQ=255)                                                                                                                                          tattatCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTGGAGGGAAAACt  <  3:2237/148‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                GGTTGATCTGTTTATTCGCGAGAATCAGCGTTTACGTCTCTCTCCTGCCGGGCATAACTTTTTACGCTATAGCCAACAAATTCTCGCGTTAGTGGATGAAGCGCGGAGCGTTGTCGCTGGCGATGAGCCGCAAGGTTTATTTTCTCTTGGTTCGCTGGAAAGCACCGCTGCAGTGCGCATTCCAGCCACGCTGGCGGAATTCAACCGTCGTTATCCCAAAATTCAGTTTTCACTTTCCACCGGCCCTTCCGGCACTATGCTGGATGGTGTACTGGAGGGAAAACT  >  NC_000913/1614935‑1615219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑