BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,186,607	C→T	P420S (CCG→TCG)	rpoC →	RNA polymerase, beta prime subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,186,607	0	C	T	100.0%	388.1 / NA	107	P420S (CCG→TCG)	rpoC	RNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (49/58); total (49/58)

Only 100 of 107 total aligned reads displayed.

AAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGAT  >  NC_000913/4186461‑4186746

aaaTGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCg                                                                                                                                           <  3:342985/149‑1 (MQ=255)

   tGGCACTGGAGCTGTTCAAACGGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTa                                                                                                                                        <  2:408960/149‑1 (MQ=255)

      cACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTActg                                                                                                                                     <  3:335496/149‑1 (MQ=255)

      cACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTActg                                                                                                                                     <  3:232871/149‑1 (MQ=255)

       aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTActgc                                                                                                                                    >  3:110798/1‑149 (MQ=255)

            aGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGCAGCTCTCGTTTGGGATATCCTGGACGAAGTTATCCGCTAACACTCGCTACTGCTGAAc                                                                                                                               >  6:221860/1‑149 (MQ=255)

                     aaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCAcc                                                                                                                       >  4:242440/1‑148 (MQ=255)

                      aCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGa                                                                                                                     >  6:323628/1‑149 (MQ=255)

                      aCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTCGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGa                                                                                                                     <  1:144488/149‑1 (MQ=255)

                          ttCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGActct                                                                                                                 <  5:461604/149‑1 (MQ=255)

                            cATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTg                                                                                                                <  6:451111/148‑1 (MQ=255)

                             aTCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCa                                                                                                              >  7:428191/1‑149 (MQ=255)

                             aTCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCAATAAAGCTGCGAAG‑AAATGGATGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGTACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCAc                                                                                                             >  4:475723/1‑149 (MQ=255)

                              tCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCAc                                                                                                             >  1:214623/1‑149 (MQ=255)

                              tCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCAc                                                                                                             >  2:371119/1‑149 (MQ=255)

                              tCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCAc                                                                                                             >  2:364114/1‑149 (MQ=255)

                                    gCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTg                                                                                                       >  5:350986/1‑149 (MQ=255)

                                     cAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAATCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGGTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                                                                                      <  5:221860/149‑1 (MQ=255)

                                     cAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCAc                                                                                                                        >  6:268237/1‑131 (MQ=255)

                                     cAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                                                                                      <  1:309959/149‑1 (MQ=255)

                                     cAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                                                                                      <  4:110798/149‑1 (MQ=255)

                                          tGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATc                                                                                                 >  3:67552/1‑149 (MQ=255)

                                            gAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCTCCGACTCTGCACCGTCTGGGTATCCa                                                                                               <  8:292524/149‑1 (MQ=255)

                                            gAACGGCGTGGGCTTGCTCCCACCAATAAAGCTGCGAACAAAATGGTTGAGCGCGAAGAAGCTGTCGTTGGGGATAACCTGGACGACGGTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATc                                                                                                 <  1:140568/147‑1 (MQ=255)

                                                  cGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAAcc                                                                                                                              >  6:101757/1‑112 (MQ=255)

                                                  cGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAAcc                                                                                                                              <  5:101757/112‑1 (MQ=255)

                                                    tGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTg                                                                                       <  7:384909/149‑1 (MQ=255)

                                                      gTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGaa                                                                                     >  3:393658/1‑149 (MQ=255)

                                                      gTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCAAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGaa                                                                                     <  6:119391/149‑1 (MQ=255)

                                                       tCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAc                                                                                    <  5:330698/149‑1 (MQ=255)

                                                       tCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAc                                                                                    <  5:323628/149‑1 (MQ=255)

                                                       tCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAc                                                                                    <  3:242440/149‑1 (MQ=255)

                                                         ttGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                                                                                      >  1:257634/1‑129 (MQ=255)

                                                         ttGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                                                                                      <  2:257634/129‑1 (MQ=255)

                                                         ttGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGATTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACAGTGCACCGACTCTCCACCGTCTGGGTATCAAGGCATTTGAACCg                                                                                  >  2:91522/1‑149 (MQ=255)

                                                               ccaccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTActgc                                                                                                                                    <  1:452487/93‑1 (MQ=255)

                                                               ccaccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTActgc                                                                                                                                    >  2:452487/1‑93 (MQ=255)

                                                                caccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                                                                                      <  8:210107/122‑1 (MQ=255)

                                                                caccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                                                                                      >  7:210107/1‑122 (MQ=255)

                                                                caccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGa                                                                           >  5:37162/1‑149 (MQ=255)

                                                                caccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGa                                                                           <  2:214623/149‑1 (MQ=255)

                                                                caccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAACGGTACTGa                                                                           >  4:51186/1‑149 (MQ=255)

                                                                 accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCgg                                                                                                                                          >  6:482712/1‑85 (MQ=255)

                                                                 accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCgg                                                                                                                                          <  5:482712/85‑1 (MQ=255)

                                                                 accaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGAt                                                                          <  4:393658/149‑1 (MQ=255)

                                                                  ccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATc                                                                         >  7:25216/1‑149 (MQ=255)

                                                                   caTTAAAGCTGCGAAGAAAATGGTTGAGCGCGATGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATc                                                                         >  1:166810/1‑148 (MQ=255)

                                                                    aTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGt                                                                                                                            <  2:300556/96‑1 (MQ=255)

                                                                    aTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGt                                                                                                                            >  1:300556/1‑96 (MQ=255)

                                                                    aTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCg                                                                        >  5:134175/1‑148 (MQ=255)

                                                                    aTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCg                                                                        >  5:109957/1‑148 (MQ=255)

                                                                     ttAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTTTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCg                                                                        >  8:297114/1‑147 (MQ=255)

                                                                        aaGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAgg                                                                    <  2:277501/148‑1 (MQ=255)

                                                                        aaGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGt                                                                   <  8:428191/149‑1 (MQ=255)

                                                                          gCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTT‑‑GATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAg                                                               <  1:512892/149‑1 (MQ=255)

                                                                             gCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGc                                                              >  3:98584/1‑149 (MQ=255)

                                                                              cGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCt                                                             <  8:25216/149‑1 (MQ=255)

                                                                               gaagaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAg                                                               >  7:180869/1‑146 (MQ=255)

                                                                               gaagaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAg                                                               <  8:180869/146‑1 (MQ=255)

                                                                               gaagaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTa                                                            >  4:439130/1‑149 (MQ=255)

                                                                                 agaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAATACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGAt                                                                          >  3:230939/1‑133 (MQ=255)

                                                                                 agaaAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAATACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGAt                                                                          <  4:230939/133‑1 (MQ=255)

                                                                                   aaaaTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAGCCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATc                                                          >  4:49312/1‑147 (MQ=255)

                                                                                      aTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTTTCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCt                                                     <  1:91522/149‑1 (MQ=255)

                                                                                      aTGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCt                                                     <  3:162930/149‑1 (MQ=255)

                                                                                        ggTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTg                                                    >  7:341603/1‑148 (MQ=255)

                                                                                             aGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCt                                                     >  6:281443/1‑142 (MQ=255)

                                                                                             aGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCt                                                     <  5:281443/142‑1 (MQ=255)

                                                                                               cgcgAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCt                                            >  2:341115/1‑149 (MQ=255)

                                                                                                  gaagtaTCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCTAACAATCGGTACTGCTTAACCGTGCACCGACTCTGCACAGTCTTGTTATCCAGGCATTTGAACCGGTACTGATCGAAGTTAAAGCTATCCAGCTTCACCCGCTTGt                                         <  4:98584/149‑1 (MQ=255)

                                                                                                  gaagaaGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGt                                         <  8:421581/149‑1 (MQ=255)

                                                                                                  gaagaaGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGt                                         >  6:351788/1‑149 (MQ=255)

                                                                                                    agaagCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGttt                                       >  4:97905/1‑149 (MQ=255)

                                                                                                       agCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg                                                                     <  2:248827/116‑1 (MQ=255)

                                                                                                       agCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAg                                                                     >  1:248827/1‑116 (MQ=255)

                                                                                                        gCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGc                                   <  4:67552/149‑1 (MQ=255)

                                                                                                         cTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCg                                  <  1:341115/149‑1 (MQ=255)

                                                                                                          tGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGGCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCgg                                 <  6:109957/149‑1 (MQ=255)

                                                                                                            tCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCgg                                 <  3:97905/147‑1 (MQ=255)

                                                                                                               tttGGGATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCata                             >  5:487902/1‑148 (MQ=255)

                                                                                                                  gggATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg                                                                                                      <  7:491340/72‑1 (MQ=255)

                                                                                                                   ggATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACg                        >  8:256122/1‑149 (MQ=255)

                                                                                                                   ggATATCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGATGCACCAGATGGTTTGTTCGGCATATaaag                        >  3:286937/1‑147 (MQ=255)

                                                                                                                     atatCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc                                                                                   >  5:143726/1‑88 (MQ=255)

                                                                                                                     atatCCTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAcc                                                                                   <  6:143726/88‑1 (MQ=255)

                                                                                                                        tCCTGGACGAAGTTATCCTCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGa                    <  3:475723/148‑1 (MQ=255)

                                                                                                                          cTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAAc                         <  5:281485/141‑1 (MQ=255)

                                                                                                                          cTGGACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAAc                         >  6:281485/1‑141 (MQ=255)

                                                                                                                             gACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCg               <  6:37162/148‑1 (MQ=255)

                                                                                                                             gACGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGa              <  6:350986/149‑1 (MQ=255)

                                                                                                                              aCGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGAt             <  1:364114/149‑1 (MQ=255)

                                                                                                                              aCGAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGGCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGAt             <  1:371119/149‑1 (MQ=255)

                                                                                                                               cGAAGTTATCCGCGAACACTCGGTACTGCTGAGCCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATg            <  3:49312/149‑1 (MQ=255)

                                                                                                                                gAAGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATgg           <  3:439130/149‑1 (MQ=255)

                                                                                                                                  aGTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTg         <  5:351788/149‑1 (MQ=255)

                                                                                                                                   gTTATCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGa        >  7:154017/1‑149 (MQ=255)

                                                                                                                                      aTCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATg            >  2:328347/1‑142 (MQ=255)

                                                                                                                                      aTCCGCGAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATg            <  1:328347/142‑1 (MQ=255)

                                                                                                                                         cgcgAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGAt  <  7:297114/149‑1 (MQ=255)

                                                                                                                                         cgcgAACACTCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGAt  <  8:252338/149‑1 (MQ=255)

AAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCATATAACGCCGACTTCGATGGTGACCAGAT  >  NC_000913/4186461‑4186746

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`