Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,376,2650GC21.3% 101.2 / 26.7 47intergenic (+29/+14)dcuD/sspBputative transporter/ClpXP protease specificity enhancing factor
Reads supporting (aligned to +/- strand):  ref base G (19/18);  new base C (4/6);  total (23/24)
Fisher's exact test for biased strand distribution p-value = 7.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGC  >  NC_000913/3376120‑3376413
                                                                                                                                                 |                                                                                                                                                    
gtCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGc                                                                                                                                                   <  2:133203/149‑1 (MQ=255)
          cAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTa                                                                                                                                         >  2:123260/1‑149 (MQ=255)
            ggAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCCAGGCGGCCTGtttt                                                                                                                                            <  1:145819/144‑1 (MQ=255)
            ggAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCCAGGCGGCCTGtttt                                                                                                                                            >  2:145819/1‑144 (MQ=255)
             gAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCCAGGCGGCCTGTTTTGTCttt                                                                                                                                      <  4:45517/149‑2 (MQ=255)
                ttGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCCAGGCGGCCTGTTTTGTCttttta                                                                                                                                   <  4:43071/149‑5 (MQ=38)
                        gTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGGGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTg                                                                                                                                                      >  6:138391/1‑122 (MQ=255)
                            cGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAAcgcg                                                                                                                          <  1:138777/146‑1 (MQ=255)
                            cGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAAcgcg                                                                                                                          >  2:138777/1‑146 (MQ=255)
                               cTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGc                                                                                                                    >  7:139143/1‑149 (MQ=255)
                                ttttGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGcc                                                                                                                   >  7:90697/1‑149 (MQ=255)
                                  ttGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCg                                                                                                                  <  8:90697/148‑1 (MQ=255)
                                   tGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCgg                                                                                                                 <  1:123260/148‑1 (MQ=255)
                                       gtggtgAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTgg                                                                                                                                                     <  2:38680/108‑1 (MQ=255)
                                       gtggtgAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTgg                                                                                                                                                     >  1:38680/1‑108 (MQ=255)
                                          gtgAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCCAGGCGGCCTGTTTTGTCtttttaat                                                                                                                                 >  8:130489/1‑119 (MQ=37)
                                          gtgAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCCAGGCGGCCTGTTTTGTCtttttaat                                                                                                                                 <  7:130489/125‑7 (MQ=37)
                                            gAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTgg                                                                                                                                                     >  3:30712/1‑103 (MQ=255)
                                            gAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTgg                                                                                                                                                     <  4:30712/103‑1 (MQ=255)
                                             aaGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGt                                                                                                                >  2:146597/1‑139 (MQ=255)
                                             aaGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGt                                                                                                                <  1:146597/139‑1 (MQ=255)
                                               gCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCCAGGCGGCCTGtttt                                                                                                                                            >  4:8625/1‑109 (MQ=39)
                                               gCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCCAGGCGGCCTGtttt                                                                                                                                            <  3:8625/109‑1 (MQ=39)
                                                                    aGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAgg                                                                                      <  2:195508/142‑1 (MQ=255)
                                                                    aGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAgg                                                                                      >  1:195508/1‑142 (MQ=255)
                                                                        ggTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTc                                                                                                                                  <  1:63921/94‑1 (MQ=255)
                                                                        ggTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTc                                                                                                                                  >  2:63921/1‑94 (MQ=255)
                                                                              gtggtgAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATc                                                                                <  1:83292/138‑1 (MQ=255)
                                                                              gtggtgAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATc                                                                                >  2:83292/1‑138 (MQ=255)
                                                                                     atatGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGtcatc                                                              <  8:139143/149‑1 (MQ=255)
                                                                                              ccACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATc                                                                                >  5:90752/1‑122 (MQ=255)
                                                                                              ccACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATc                                                                                <  6:90752/122‑1 (MQ=255)
                                                                                               cacaATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGAt                                                      >  8:108130/1‑147 (MQ=255)
                                                                                               cacaATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGAt                                                      <  7:108130/147‑1 (MQ=255)
                                                                                                    tCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCtt                                               >  2:2869/1‑149 (MQ=255)
                                                                                                        gCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATc                                                     <  1:96968/139‑1 (MQ=255)
                                                                                                        gCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATc                                                     >  2:96968/1‑139 (MQ=255)
                                                                                                            ttttATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCAt                                       >  6:11426/1‑149 (MQ=255)
                                                                                                                    aGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGc                                                 >  2:31774/1‑131 (MQ=255)
                                                                                                                    aGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGc                                                 <  1:31774/131‑1 (MQ=255)
                                                                                                                    aGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAAcc                               <  5:11426/149‑1 (MQ=255)
                                                                                                                          aagtaataCAAAACAGGCCCAGGCGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAAc                                >  1:164406/8‑142 (MQ=38)
                                                                                                                          aagtaataCAAAACAGGCCCAGGCGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAAc                                <  2:164406/135‑1 (MQ=38)
                                                                                                                             cacgACAAAACAGGCCGCCTGGGCCGGGTTTGTATTACTTCACAACGCGTAAGGCAGGTCGACCTCCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCTTGATCGTGATCTGGCTTGTCGCCATCAATAACCGACAt                          <  8:36509/142‑1 (MQ=255)
                                                                                                                             aaaGACAAAACAGGCCGCCTGGGCCTGTTTAGTATTACTTCACAACGCGTAATGCCGGTCGACCCCCGCCTGGGGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCAGCGTGATCTGGCTTGTCGCCATCAATAACCGCCAt                          >  7:36509/1‑145 (MQ=255)
                                                                                                                                   aaaaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTc                 <  4:149043/148‑1 (MQ=255)
                                                                                                                                   aaaaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTc                 >  3:149043/1‑148 (MQ=255)
                                                                                                                                   aaaaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCg                >  6:42332/1‑149 (MQ=255)
                                                                                                                                     aaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATc                                      >  7:55553/1‑125 (MQ=255)
                                                                                                                                     aaCAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATc                                      <  8:55553/125‑1 (MQ=255)
                                                                                                                                        aGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTc           <  5:42332/149‑1 (MQ=255)
                                                                                                                                          gccgccTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTg         >  8:22943/1‑149 (MQ=255)
                                                                                                                                                 gggCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGc  >  7:79422/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GTCGTTACGTCAGGAATTGCAGGCGTTTCGCCTTTTGCGGTGGTGAAGCGGACAGCGATCCCCATGGCAGTCGGTTTCGTGGTGAATATGATTGCCACAATCACGCTATTTTATTAAGTCATTAAAAAGACAAAACAGGCCGCCTGGGCCTGTTTTGTATTACTTCACAACGCGTAATGCCGGTCGACCACCGCGTGGTGGCTGCGGAGGTTCATCGTCAGGATGAGTGTCATCATCGTGATCTGGCTTGTCGCCATCAATAACCGACATAACGGTTTCGTTGTCTGCCGATGC  >  NC_000913/3376120‑3376413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑