Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,890,075	IS5 (–) +4 bp	coding (1346‑1349/1416 nt)	tnaA →	tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2290113	NA (NA)	58 (1.600)	39/240	0.1	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	3890075 =	0 (0.000)					coding (1346/1416 nt)	tnaA	tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
*	?	NC_000913	3652036 =	NA (NA)	70 (1.930)	56/240	0.0	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	= 3890078	0 (0.000)					coding (1349/1416 nt)	tnaA	tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent

CGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2289966‑2290113 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTC  >  NC_000913/3890075‑3890219                                                                                                                                                                                                                                                                                                       CGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCTCTGTAGGGGAAGGTAAGTTGATGTCTCATGATGAACCCTGTTCTCTGGCTCCAGATGACAAACATGATCTCATATCAGGGACGTGTTCGCACCTTCCT                                                                                                                                                  <  7:161851/149‑1  GGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                  >  7:164792/1‑148      CAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAG                                                                                                                                             <  5:148645/149‑1                TCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAA                                                                                                                                              >  6:19203/1‑138                TCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAA                                                                                                                                              <  5:19203/138‑1                  TTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                  <  2:167832/132‑1                  TTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                  >  1:167832/1‑132                                GGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGATCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAA                                                                                                                   <  4:83758/149‑1                                         TGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATT                                                                                                                                         <  4:30956/118‑1                                         TGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATT                                                                                                                                         >  3:30956/1‑118                                              AATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAGCATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATT                                                                                                              >  1:116722/1‑140                                              AATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAGCATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATT                                                                                                              <  2:116722/140‑1                                                     TGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                  >  1:61359/1‑97                                                     TGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                  <  2:61359/97‑1                                                              AGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACC                                                                                                 >  6:176506/1‑137                                                              AGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACC                                                                                                 <  5:176506/137‑1                                                                       GATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAA                                                                                                                   <  1:108169/110‑1                                                                       GATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAA                                                                                                                   >  2:108169/1‑110                                                                             TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                  <  2:133663/73‑1                                                                             TCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                  >  1:133663/1‑73                                                                             TCATGATGAACCCTGTTCTATGGCACCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                  <  8:82580/73‑1                                                                             TCATGATGAACCCTGTTCTATGGCACCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCT                                                                                                                                                  >  7:82580/1‑73                                                                               ATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAAC                                                                                                                        >  8:66196/1‑97                                                                               ATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAAC                                                                                                                        <  7:66196/97‑1                                                                               ATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT                                                                                                                                                 <  2:56592/72‑1                                                                               ATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTT                                                                                                                                                 >  1:56592/1‑72                                                                                TGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACTGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAG                                                                                 >  6:106207/1‑135                                                                                TGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACTGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAG                                                                                 <  5:106207/135‑1                                                                                   TGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTA                                                                   >  4:159289/1‑146                                                                                    GAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTA                                                                                                                <  3:140505/100‑1                                                                                    GAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTA                                                                                                                >  4:140505/1‑100                                                                                     AACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCACACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATT                                                                                                              >  6:189366/1‑101                                                                                     AACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCACACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATT                                                                                                              <  5:189366/101‑1                                                                                        CCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAA                                                                                     >  7:90991/1‑123                                                                                        CCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAA                                                                                     <  8:90991/123‑1                                                                                                ATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTCGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAG                                                    >  6:185326/1‑148                                                                                                   GCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAG                                                              <  6:70486/135‑1                                                                                                   GCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAG                                                              >  5:70486/1‑135                                                                                                    CTCCAGATGACAAACATGATCTCATATCAGGGACTCGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGT                                               <  5:185326/149‑1                                                                                                       CAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTAC                                                                                                                            <  4:93781/69‑1                                                                                                       CAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTAC                                                                                                                            >  3:93781/1‑69                                                                                                          ATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCA                                         >  1:133823/1‑149                                                                                                          ATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCA                                         >  8:126885/1‑149                                                                                                               AAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTC                                    >  2:86383/1‑149                                                                                                               AAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTC                                    >  2:89734/1‑149                                                                                                                       TCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCat                             >  3:38808/1‑146                                                                                                                       TCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGAT                                                 >  1:116482/1‑128                                                                                                                       TCTCATATCAGGGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGAT                                                 <  2:116482/128‑1                                                                                                                                  GGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTT                                                                              >  1:92967/1‑88                                                                                                                                  GGACTTGTTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTT                                                                              <  2:92967/88‑1                                                                                                                                         TTCGCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAA          >  7:20678/1‑149                                                                                                                                            GCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAG       >  4:84037/1‑149                                                                                                                                            GCACCTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATA        <  4:38808/148‑1                                                                                                                                                CTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCTCTTCACCGCAATACTTAAAGAAGTTTAATTAATTCTACAGAGTTGCTATAATGATGTTAGACACTCTCTTACCCTACATCCTCAATAt                <  1:107840/136‑2                                                                                                                                                CTTCCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAA                >  2:107840/1‑136                                                                                                                                                   CCTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTC  <  8:20678/147‑1                                                                                                                                                    CTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTC  >  1:53170/1‑146                                                                                                                                                    CTTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTC  <  2:53170/146‑1                                                                                                                                                                                                                                                                                                       CGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2289966‑2290113 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAAAGGATTAACCTTTACGTACGAACCGAAAGTATTGCGTCACTTCACCGCAAAACTTAAAGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGCCTTC  >  NC_000913/3890075‑3890219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.