| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,186,577 | G→C | D410H (GAT→CAT) | rpoC → | RNA polymerase, beta prime subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,186,577 | 0 | G | C | 98.2% | 199.7 / ‑5.9 | 57 | D410H (GAT→CAT) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (29/27); minor base T (0/1); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCA > NC_000913/4186434‑4186717 | gTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATc < 1:133247/149‑1 (MQ=255) ccGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCcgcg > 8:91500/1‑148 (MQ=255) gaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGa > 3:104834/1‑144 (MQ=255) gaaAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGa < 4:104834/144‑1 (MQ=255) aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAg > 5:109293/1‑125 (MQ=255) aCTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAg < 6:109293/125‑1 (MQ=255) gAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGaa > 4:141727/1‑149 (MQ=255) aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTg > 4:169678/1‑105 (MQ=255) aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTg < 3:169678/105‑1 (MQ=255) aaaCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCAcc > 1:98762/1‑149 (MQ=255) aCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGc > 5:95311/1‑144 (MQ=255) aCCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGc < 6:95311/144‑1 (MQ=255) gTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgc > 5:100864/1‑131 (MQ=255) gTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTActgc < 6:100864/131‑1 (MQ=255) gTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGActc < 7:91500/149‑1 (MQ=255) gTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGActc < 1:44372/149‑1 (MQ=255) tCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCAc > 2:44821/1‑149 (MQ=255) cTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTgg < 2:56747/95‑1 (MQ=255) cTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTgg > 1:56747/1‑95 (MQ=255) tACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGa > 7:78806/1‑95 (MQ=255) tACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGa < 8:78806/95‑1 (MQ=255) cGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCAcc < 1:44821/146‑1 (MQ=255) aGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTggg > 7:70507/1‑148 (MQ=255) gCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg < 8:99490/146‑1 (MQ=255) gCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg > 7:99490/1‑146 (MQ=255) aaCTGCGTGGTCTTGCTACGACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAg < 3:141727/149‑1 (MQ=255) cTGCGTTGGATTGCTACTACCATTTAATCTGCGTAGAAAATGGTTGTGCGCGAAGAAGCTGTCGTTTGGTATATCCTGGAAGAAGTTATCCGCGAACACCCGGTAGTGGTTAAACTTGCACCGACTCTTCAGCGTCTgg < 8:121046/139‑1 (MQ=255) cTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTTGCATATCCAGTACGAAGTTATCCGCGAACACCCCTTACGGCTGAACCGTTCACCGACTCTGCACCGTCggg > 7:121046/1‑139 (MQ=255) gTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGaa < 2:98762/149‑1 (MQ=255) tCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAt > 5:154322/1‑143 (MQ=255) tCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCAt < 6:154322/143‑1 (MQ=255) tCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAAc < 8:70507/149‑1 (MQ=255) gCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCgg > 4:84746/1‑148 (MQ=255) tACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTAc > 6:9750/1‑149 (MQ=255) accaccATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACAcc > 8:11632/1‑85 (MQ=255) accaccATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACAcc < 7:11632/85‑1 (MQ=255) ccaccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTAc > 5:139907/1‑147 (MQ=255) ccaccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTAc < 6:139907/147‑1 (MQ=255) caccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg < 5:31845/122‑1 (MQ=255) caccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTgg > 6:31845/1‑122 (MQ=255) ccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATcc < 4:92325/126‑1 (MQ=255) ccaTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATcc > 3:92325/1‑126 (MQ=255) aTTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATAACATGGACGAAGTTATCCGCGAACACCCGGTACAGCAGAACCGTGCACCGACTCAGCACCCTCTGGGTATCCAGGCATTTGAAAAGGAACTGATCg > 8:108767/1‑148 (MQ=255) gCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTaa < 5:9750/149‑1 (MQ=255) cTGCGAAGAAAATGGTTGCGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGAAGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGa > 2:121058/1‑142 (MQ=255) cTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGa < 1:121058/142‑1 (MQ=255) gCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTg > 6:112114/1‑135 (MQ=255) gCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTg < 5:112114/135‑1 (MQ=255) aaaaTGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCt < 3:94710/101‑1 (MQ=255) aaaaTGGTTGAGCGAGAAGAAGCTGTCGTTTGGCATATCCTGGACTAAGTTATCCTCGAACACACGGTACTGCTGAACCGTGCACCGACTCTGCACCGACt > 4:94710/1‑101 (MQ=255) aaTGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTc < 3:73882/98‑1 (MQ=255) aaTGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTc > 4:73882/1‑98 (MQ=255) aTGGTTGAGCGCGAAGAAGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCt > 7:57954/1‑149 (MQ=255) gaagaaGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCa < 2:63330/80‑1 (MQ=255) gaagaaGCTGTCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCa > 1:63330/1‑80 (MQ=255) tCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGc < 8:57954/148‑1 (MQ=255) tCGTTTGGCATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCa > 7:99162/1‑149 (MQ=255) | GTCTGCATCAGTGCGGTCTGCCGAAGAAAATGGCACTGGAGCTGTTCAAACCGTTCATCTACGGCAAGCTGGAACTGCGTGGTCTTGCTACCACCATTAAAGCTGCGAAGAAAATGGTTGAGCGCGAAGAAGCTGTCGTTTGGGATATCCTGGACGAAGTTATCCGCGAACACCCGGTACTGCTGAACCGTGCACCGACTCTGCACCGTCTGGGTATCCAGGCATTTGAACCGGTACTGATCGAAGGTAAAGCTATCCAGCTGCACCCGCTGGTTTGTGCGGCA > NC_000913/4186434‑4186717 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |