New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1159325 =	53 (1.170)	5 (0.120)	5/262	5.5	9.3%	intergenic (+23/+37)	ptsG/fhuE	fused glucose‑specific PTS enzymes: IIB component/IIC component/ferric‑rhodotorulic acid outer membrane transporter
	?	NC_000913	1159347 =	48 (1.140)					intergenic (+45/+15)	ptsG/fhuE	fused glucose‑specific PTS enzymes: IIB component/IIC component/ferric‑rhodotorulic acid outer membrane transporter
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGA  >  NC_000913/1159186‑1159462                                                                                                                                            |                                                                                                                                          cTGAAGGAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAgg                                                                                                                                        <  2:215576/143‑1 (MQ=255)   gaagaaACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACCAAGGCa                                                                                                                                      <  1:105027/143‑1 (MQ=255)      gaaACTGTGCGCAGCGGGCGTAGTGGTTGCTAGTGCCGGTGGTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGcc                                                                                                                                   <  1:55339/143‑1 (MQ=255)           tGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATg                                                                                                                              >  2:40714/1‑143 (MQ=255)                ccAGAGGGCGCGGTGGTGGCTGGTTCTGGTGTTCAGGCGCTTTTCGGTACTACATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTCAGGCAGCCATCTGGCTGc                                                                                                                         <  2:710351/142‑1 (MQ=255)                   gCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCtt                                                                                                                      <  1:714691/143‑1 (MQ=255)                     ggggGTAGTGGTTGCGGGTTCGGGTGTTGAGGCGATGTGCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCtttt                                                                                                                    <  1:241308/143‑1 (MQ=255)                     gggCGTAGTGGTTGCTGGTTCTGGTGTTCAGGAGATATTGACGACTATATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCtttt                                                                                                                    <  2:802091/143‑1 (MQ=255)                      ggCGTAGTGGTGGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCttttt                                                                                                                   <  2:430016/143‑1 (MQ=255)                      agCGTAGTGGTTGCTGTTGTTCGTGTTCACGCGTTTTTCGCTACTAAATCTGATAACCTGAAAACCGAGAGGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCttttt                                                                                                                   <  2:40398/142‑1 (MQ=255)                       gCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGCGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGCCTAAGGCAGCCAGATgcctgcctctgtt                                                                                                                  >  1:166621/1‑138 (MQ=255)                          tAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACa                                                                                                               <  1:263958/143‑1 (MQ=255)                                    tGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTc                                                                                                     >  1:731553/1‑143 (MQ=255)                                          tGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGTGGTGGCTGACCACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAAtt                                                                                               <  1:603005/143‑1 (MQ=255)                                          tGGGGTTTAGGCGATTTTCGGTACTAAAGCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAAtt                                                                                               <  1:83482/143‑1 (MQ=255)                                            gtgtTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGa                                                                                             >  1:435688/1‑143 (MQ=255)                                            gggtTCAGGCGCTTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGa                                                                                             <  2:640535/141‑1 (MQ=255)                                                   ggCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTCTTTTACAGGTGTCATTCAGAATTGATACCTGc                                                                                      >  2:696568/1‑143 (MQ=255)                                                   cgCGATTGTCGGTGCTAGATCCGATAACCTGAACAGCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGc                                                                                      <  2:313938/142‑1 (MQ=255)                                                    gCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATGCGTAAGACGTTGGGGAGACTAAGGCAGCCAAGTGGCTGCCTTTGTCACAGGTGCTATGGAGAATTGATACGTGcc                                                                                     >  2:311639/1‑143 (MQ=255)                                                     cGATGTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCg                                                                                    <  1:114182/143‑1 (MQ=255)                                                         tttCGGTACTAAATCCGATAACCTGAACACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTaa                                                                                >  1:527382/1‑143 (MQ=255)                                                         tttCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTAACTTAACCAATGTGGTTATTCAGAATTGATAAGCGCCGGTaa                                                                                >  2:582198/1‑143 (MQ=255)                                                             ggTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGAGTTATTCAGAATTGATACGTGCCGGTAATGCt                                                                            <  2:433349/143‑1 (MQ=255)                                                               tACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTCATGCTGa                                                                          >  1:208012/1‑143 (MQ=255)                                                               tACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGa                                                                          <  1:483543/143‑1 (MQ=255)                                                               tACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGa                                                                          >  1:195608/1‑143 (MQ=255)                                                                aCTAAATCCTATAACCTGACAACCGAGATGGATGAGTACATCCGTAACCACTAATTCGTAAGACGTTGGGGAGACTAAGGCAGTCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGaa                                                                         <  1:232966/143‑1 (MQ=255)                                                                  tAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAGAt                                                                       >  1:363243/1‑143 (MQ=255)                                                                        cGATAACCTGAAAACCGAGATGGATGAGTAAATCCGTAACCACTAAGCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTAcgcg                                                                 <  2:70083/143‑1 (MQ=255)                                                                         gATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTAGGGGAGACTAAGGCAACCAGATGGCTGCCAGATTTAGAGGAGTTATTCAGAATTAATATGGTCCTGTAAAACCGAAATTACGCgg                                                                >  2:439805/1‑143 (MQ=255)                                                                          atgaCCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgt                                                               <  2:382578/140‑1 (MQ=255)                                                                          atatcttGACAACCGAGATGGATCAGTACAGCAGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGgt                                                               <  2:104420/137‑1 (MQ=255)                                                                              cccgAAAACCGAGATGGGTGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGCGACGAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATCCGTGCCGGTAATGCTGAAATTACGCGGTGTGc                                                           <  2:432367/140‑1 (MQ=255)                                                                               cTGAAAACCGAGATGGATGGGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGcc                                                          <  2:120454/143‑1 (MQ=255)                                                                               cTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGcc                                                          >  1:617414/1‑143 (MQ=255)                                                                               cTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTAATCAGAATTGATACGTGCCGGTAATGGTGAAATTAAGCGGAGCGcc                                                          >  2:778868/1‑143 (MQ=255)                                                                                    aaCCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGGCGTGGGGGAGACTAAGGCAGCCAGATGGCTGGCTTTTTTACAGGTGTTATTCAGAATTGGTACGTGCCGGTAAGGCGGAAATTACGCGGTGTGCCGTAGa                                                     >  1:404180/1‑143 (MQ=255)                                                                                    aaCCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACACGTGTGATTAAGAATTGATACGTGCCGGTAATGCTGACATTACGCGGTGTGCCggtga                                                     >  1:470120/1‑139 (MQ=255)                                                                                                       aTCCGTAACCACTTATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGtt                                  <  2:771017/143‑1 (MQ=255)                                                                                                        tCCGTAACCACTAATCCGGAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTg                                 <  1:122455/143‑1 (MQ=255)                                                                                                        gtggTAACCGCGAATGGGTAAGACGGTGGGGAGGCTGCGGCGGCCAGATGGCTGCCTTTTGTGCAGGTGTTATTCGGCATCGATCCGTGCGGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTg                                 <  2:291722/140‑1 (MQ=255)                                                                                                          cGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACggtggt                               >  1:33891/1‑143 (MQ=255)                                                                                                               ccACTAATCCGTAAGACGTTGGGGAGACGAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGt                          <  1:543084/143‑1 (MQ=255)                                                                                                                cACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTGGGTATCGTa                         >  2:38042/1‑143 (MQ=255)                                                                                                                 aCTAATCCGTAAGACGTTGGGGAGACTAAGTCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTTTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAg                        <  1:327476/143‑1 (MQ=255)                                                                                                                 aCTAATCCGTAAGACGGTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAg                        <  1:631834/143‑1 (MQ=255)                                                                                                                           aaGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGa              >  1:574699/1‑143 (MQ=255)                                                                                                                              aCGTTGGGGAGACTCAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTCATGCTGAAATTACGCGGTGTGCCGTAGCCGATAGAACCTTCCACGTTGGTATCGTCGGTTTTGTCGAACa           >  1:369164/1‑143 (MQ=255)                                                                                                                              aCGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACa           <  2:731553/143‑1 (MQ=255)                                                                                                                                 ttGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGt        >  1:800348/1‑143 (MQ=255)                                                                                                                                    gggAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGGGTTATTCAGATTTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGGAGGTTTTGTCGAACAGGTTAt     >  1:426037/1‑143 (MQ=255)                                                                                                                                       agaCTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGa  <  1:40714/143‑1 (MQ=255)                                                                                                                                            |                                                                                                                                          CTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAATCCGATAACCTGAAAACCGAGATGGATGAGTACATCCGTAACCACTAATCCGTAAGACGTTGGGGAGACTAAGGCAGCCAGATGGCTGCCTTTTTTACAGGTGTTATTCAGAATTGATACGTGCCGGTAATGCTGAAATTACGCGGTGTGCCGTAGACGATAGAACCTTCCACGTTGGTATCGTAGGTTTTGTCGAACAGGTTATTGA  >  NC_000913/1159186‑1159462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.