Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,968,509 Δ1 bp coding (594/1104 nt) wecA → UDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,968,5090A.95.5% 177.4 / 0.9 45coding (594/1104 nt)wecAUDP‑GlcNAc:undecaprenylphosphate GlcNAc‑1‑phosphate transferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base . (17/25);  total (19/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCAAAACCCATCCCATCAGCC  >  NC_000913/3968375‑3968645
                                                                                                                                      |                                                                                                                                        
aTGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGGCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATg                                                                                                                                 <  1:623718/143‑1 (MQ=255)
 tggtggATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGTAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGc                                                                                                                                <  1:62939/143‑1 (MQ=255)
 tGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGc                                                                                                                                >  1:721499/1‑143 (MQ=255)
   gTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCtt                                                                                                                              >  1:311588/1‑143 (MQ=255)
    ttGATGGCAGTGATGGCTTGTTGGGTGGGTTGTCCTGCGACTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTa                                                                                                                             <  1:690394/143‑1 (MQ=255)
         gtcATTGATGGCTTGCTGGGGGGGTTGTCCTGCGTCGAGGTAGCAGCAATCGGTATGATTGTGTGGTCCAACTTGCAAACCGGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCtt                                                                                                                        <  1:234966/141‑1 (MQ=255)
         ggCAGTGATGGCGTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCtt                                                                                                                        <  2:48761/143‑1 (MQ=255)
            aTTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAACAGGGACGATTTTGTGGTTCGGCGGGCAAACCAGCCTCGCAAGCTGGTGCTTTGCGCAGCTCGCCGCCGTCATGCACCCATCATCGTTAACCGTggg                                                                                                                     >  2:848755/1‑142 (MQ=255)
                  ggCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCCGCAATCGGTCTGCTTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCGTTGCGATGATCGCCGCCATCCTCCCTACATCACGCGTGACCTCGGTACCCTg                                                                                                               >  1:327155/1‑143 (MQ=255)
                   gCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTgg                                                                                                              <  2:626465/143‑1 (MQ=255)
                          ggggggGTTGTCGGGCGCCTCGTCTGGAGCAATGGGTATGATTTTGTGGGTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTcgccg                                                                                                       <  2:609794/143‑1 (MQ=255)
                              gggTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCACTCTTAACCTTCGTCTCCTGGGTCGCCGCCAc                                                                                                   >  1:411280/1‑143 (MQ=255)
                                 ttGTCCTGCGTCTAGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACaaa                                                                                                <  1:161755/143‑1 (MQ=255)
                                 ttGTCCTGCGTCGCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACaaa                                                                                                <  1:46667/143‑1 (MQ=255)
                                 ttGTCCGGCGTCTAGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCGTGGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACaaa                                                                                                <  1:166509/143‑1 (MQ=255)
                                     ccTGCGGCTCGTTTGCCGCCAGCCGGATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCt                                                                                            <  2:397597/143‑1 (MQ=255)
                                        gCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTa                                                                                         >  2:963944/1‑143 (MQ=255)
                                         cGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGAGGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGCATCCTGGGTCGCCGCTACAAACGCTTTAt                                                                                        >  1:328448/1‑143 (MQ=255)
                                          gTCTCGTTTGCACCAATCGGGATGATTTTGGCGTGCGTTGGGCAAACCGTCCTCGCGATCTGGTGCTTAGAGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTAGCCTGGGTCGCCGCTACAAAGTCTTTATg                                                                                       <  2:247862/143‑1 (MQ=255)
                                                    cagcaATCGGTATGACTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCgg                                                                             <  1:765814/143‑1 (MQ=255)
                                                     agcaATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGAGATGCggg                                                                            >  1:831314/1‑143 (MQ=255)
                                                     agcaATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCGCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCggg                                                                            <  1:161082/143‑1 (MQ=255)
                                                         aTCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTTACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGt                                                                        <  1:828816/143‑1 (MQ=255)
                                                                tGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAACGTCTTTATGGGTGATGCGGGCAGTACGCTgc                                                                 >  1:538532/1‑142 (MQ=255)
                                                                tGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGa                                                                 >  1:730695/1‑143 (MQ=255)
                                                                           ttCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTAcc                                                      <  1:848932/143‑1 (MQ=255)
                                                                               aCGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGAGGGCAGTACGCTGACTGGGGTTACCGttc                                                  >  2:699563/1‑142 (MQ=255)
                                                                                cGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTTGGTTGCCGCTACAAAGTCTTTATGTGGGATGCGGGTATTACGCTGATTGGTTTTACCGTTAt                                                 <  1:555017/143‑1 (MQ=255)
                                                                                   gCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTg                                              >  1:183499/1‑143 (MQ=255)
                                                                                      aaCCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGAt                                           <  1:514794/143‑1 (MQ=255)
                                                                                             gtCGCTATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTc                                    <  2:171075/142‑1 (MQ=255)
                                                                                             cTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTc                                    <  2:183499/143‑1 (MQ=255)
                                                                                              tCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGCTCCTGCTCg                                   >  1:302105/1‑143 (MQ=255)
                                                                                                          gCTTTGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAgg                       <  1:568537/143‑1 (MQ=255)
                                                                                                           cTTTGCGATGATCGCCGCCATCCTGCCGACACGAGGCATAAGCGTGGGAACGTGGGTCGCCGCTACGAAGTGTTTATGGGGGAGGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAggg                      <  1:228858/143‑1 (MQ=255)
                                                                                                            tttGCGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTCCCGTTATCTGGCTCCTTCTCGTAATGAccccgtgc                     >  1:41321/1‑138 (MQ=255)
                                                                                                                cgctgatCGCGGCCGTCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTGTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCaaaa                 <  2:387908/140‑1 (MQ=255)
                                                                                                                cGATGATCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGGTGCGGGCCGTACGCTGATTGGTTTTCCCGTTATCTGGATCCTGCCCGAAACGACCCAGCGCaaaa                 >  1:564100/1‑143 (MQ=255)
                                                                                                                cGATGATCGCCGCCATCCTGCAGACTTCATGCTGAACCGGTGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCaaaa                 <  1:375019/143‑1 (MQ=255)
                                                                                                                  atgatCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTCATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGTTCCTGCTCGAAACGACCCAGAGCCAAAcc               >  2:785890/1‑143 (MQ=255)
                                                                                                                  atgatCGCCGCCATCCTGCCTACATCATGCTTAACATTCATCGACTACCAAGCGCCTACTAAGTCTGTATGGGTGATGCGGGCAGTACGCCGACGGGTAGCAGCGTTATCTGGATCCTGCTCGAAACGACACAGGGCAAAAcc               >  2:662708/1‑143 (MQ=255)
                                                                                                                      tCGCCGCCATCCTGCCTACATCATGCTTAACCTTGGGATCCTGGGTCCCCGCTACAAAGTCTTTATGTGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTCCTCGAAACGACCCAGGGCAAAAcccatc           <  1:662708/143‑1 (MQ=255)
                                                                                                                       cgccgcCATCCTGCCTACCTCATGCTTAACCTTGGCATCCTGTGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCAAAAcccatcc          <  1:53399/143‑1 (MQ=255)
                                                                                                                            ccATCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTGTTACCGTTATCTGGATCATGCTCGAAGCGACCCAGCGCCAAACCCATCCCATCa     >  2:909629/1‑143 (MQ=255)
                                                                                                                               tCCTGCCTACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATTCGGGCAGTACGCTGCTTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCCCGACCACTCCCATCAGca  >  2:816139/1‑142 (MQ=255)
                                                                                                                                      |                                                                                                                                        
ATGGTTGATGGCATTGATGGCTTGCTGGGCGGGTTGTCCTGCGTCTCGTTTGCAGCAATCGGTATGATTTTGTGGTTCGACGGGCAAACCAGCCTCGCAATCTGGTGCTTTGCGATGATCGCCGCCATCCTGCCATACATCATGCTTAACCTTGGTATCCTGGGTCGCCGCTACAAAGTCTTTATGGGTGATGCGGGCAGTACGCTGATTGGTTTTACCGTTATCTGGATCCTGCTCGAAACGACCCAGGGCAAAACCCATCCCATCAGCC  >  NC_000913/3968375‑3968645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: