Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	150.4 / NA	49	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/18);  total (31/18)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG  >  NC_000913/2132650‑2132924                                                                                                                                          |                                                                                                                                          tCTGGTAGAGGGTAAACAGCTGATATTTCTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                      <  1:710197/143‑1 (MQ=255)   tcgTAGAGGGTAAACAGCTGATATTTCTTGCTGGCGCGGTCGCCTTTGTCTTCGTGCTTGCGGTAAGNGCGGAAGTACCCGGACAATTTCCTCGGCGACCAGGTGATCTGCATTCCACCGTCACTGACCTGCACGCTCCGCGt                                                                                                                                    >  2:575599/3‑143 (MQ=255)    ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                   <  1:676824/143‑1 (MQ=255)           ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt                                                                                                                            <  2:672792/143‑1 (MQ=255)                aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGTTCTGCATTTCACCGTGCTTGATATGCAGGCTCTACGTCGActcgcctagt                                                                                                                       >  1:84744/1‑136 (MQ=255)                aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATCTGCCGGCTCTGCGTCGCCTCGTCTGCt                                                                                                                       >  1:746880/1‑143 (MQ=255)                aCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACt                                                                                                                       >  1:708153/1‑143 (MQ=255)                 catgtgctatTTTTTGCTGGCGCGGGCGAATTTGGCTGTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt                                                                                                                      <  2:288458/136‑1 (MQ=255)                     tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                  >  1:396632/1‑143 (MQ=255)                          ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                             >  1:343253/1‑143 (MQ=255)                          ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                             <  2:889712/143‑1 (MQ=255)                           tttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTc                                                                                                            >  1:491030/1‑143 (MQ=255)                                        ggTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc                                                                                               >  1:778488/1‑143 (MQ=255)                                          tCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGCATTTTTTCGGTGCCGAGGTGAGCTGCATTTCACCGTGATTGATATGCAGGCGCTGAGTCGCCTCTTCTACTGTCCACGGTTCGCCCTACTctacgac                                                                                             >  2:736078/1‑143 (MQ=255)                                                      ttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGATGTTTGTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                                 <  2:604035/143‑1 (MQ=255)                                                        gtgCTCGCGGTAAAAGGGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa                                                                               <  1:895816/143‑1 (MQ=255)                                                              gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGAAGGCTCTGCGTCGCGTCTTATACGTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGt                                                                         >  2:212770/1‑143 (MQ=255)                                                              gCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCATGATTGATATGCAGGCTCTGCGTCGCCTCTTCGACTTTCCACGGTTCGCCGGACTCCCCCCACATCCGCAGGAAGCTGTCGt                                                                         >  2:326805/1‑143 (MQ=255)                                                                ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCATCTTCTACTTTCCACGGTTCGCAGTACTCCACAAACATCCGCAAGAAGATGtagaaa                                                                       >  2:287426/1‑138 (MQ=255)                                                                 gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCGTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGAGGTCGTAAt                                                                      >  2:641619/1‑143 (MQ=255)                                                                  tAAAAGTGGAAGTACCCGGAGACTTTTTACGGTGACGAGGTGATCTGAATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTACTACTATCGACGGATCGCGGTACTGCACCACACCCCGCACGACGATGGCGCAATc                                                                     >  2:403005/1‑143 (MQ=255)                                                                  tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTACTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGGTGTCGTAATc                                                                     >  2:717246/1‑143 (MQ=255)                                                                      aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                                 <  2:708153/143‑1 (MQ=255)                                                                       gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCCCTGCGTCGCCTCTTCTACTCCCCCCGGTCCGCCGTACTCCACCACCACCCGCCGGACGCCGCCGCAATCCtgtg                                                                >  1:941208/1‑143 (MQ=255)                                                                            aGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCt                                                           <  2:343253/143‑1 (MQ=255)                                                                                cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCa                                                       <  1:477912/143‑1 (MQ=255)                                                                                 ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGCTGTCGTCATCCTGTGCCGCTTTCAg                                                      >  1:636068/1‑143 (MQ=255)                                                                                    gttcaTTTTTTCGTTGACGAGGGGATCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc                                                   <  2:579520/139‑1 (MQ=255)                                                                                    gagaATTTTTTCGGTGACGAGGTGATCTGCATTGCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc                                                   <  2:809637/143‑1 (MQ=255)                                                                                          tttttCGGTGACGAGGTGATCTTCATTTCACCGTGATTGATATGCAGGCTCTGCGTTGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGGAGGAGGATGTCGTAATCCTGTACCGCTTTCCATTCGATATc                                             >  2:768188/1‑143 (MQ=255)                                                                                                        ggTGATCTGCATTGGAGCGGGCTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTGCCACGGTTCGCCGTACTCCACCAGCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt                               <  1:837860/143‑1 (MQ=255)                                                                                                         gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTTTCGAACAAACAGTCttt                              >  2:1014193/1‑143 (MQ=255)                                                                                                          tGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTg                             >  1:249511/1‑143 (MQ=255)                                                                                                             tgtGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                          <  2:743243/141‑1 (MQ=255)                                                                                                             tCTGCATTTCACCGTGATTGATCTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCTTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                          <  2:819461/143‑1 (MQ=255)                                                                                                              cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac                                                                                                >  2:393062/1‑72 (MQ=255)                                                                                                              cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac                                                                                                <  1:393062/72‑1 (MQ=255)                                                                                                              cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCAGGCAGATGTCGTACTCCTGTGCCGCCCTCAGCTCGGTCCCGACCAACCCCCCtttcagac                         >  1:447702/1‑138 (MQ=255)                                                                                                                gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCCCCCTACTATATCTGCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGc                       >  1:637899/1‑143 (MQ=255)                                                                                                                  aTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                         <  1:811112/107‑1 (MQ=255)                                                                                                                  aTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                         >  2:811112/1‑107 (MQ=255)                                                                                                                       aCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGACACGCCATGccc                >  1:677370/1‑143 (MQ=255)                                                                                                                             attgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACACACACTCTTTGAAACGACATTCCCAGGTaa          >  2:922060/1‑143 (MQ=255)                                                                                                                             attgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGCAACGCCATGCCCCGGTaa          >  2:154610/1‑143 (MQ=255)                                                                                                                               tgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGACCAAACACTCTTTGAAACGCCATGCCCAGGTAAAg        >  1:650061/1‑143 (MQ=255)                                                                                                                                gatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGa       >  2:184838/1‑143 (MQ=255)                                                                                                                                    tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATCCTCTGCCGCTTTCAGTTCGGTATCGAACAAACACCCTTTGAAACGCCCAGCCCAGGCaacgccccg   >  2:183638/1‑136 (MQ=255)                                                                                                                                    tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTg   >  1:561433/1‑143 (MQ=255)                                                                                                                                     gCAGGCGCTGCGTCGCCTCTGCCACTTTCCTCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGGAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTgg  <  2:396632/143‑1 (MQ=255)                                                                                                                                          |                                                                                                                                          TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGG  >  NC_000913/2132650‑2132924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑