Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,316,681	A→G	T169A (ACC→GCC)	rcsB →	response regulator in two‑component regulatory system with RcsC and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,316,681	0	A	G	100.0%	188.5 / NA	64	T169A (ACC→GCC)	rcsB	response regulator in two‑component regulatory system with RcsC and YojN
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (29/35);  total (29/35)

CAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATA  >  NC_000913/2316546‑2316819                                                                                                                                        |                                                                                                                                           cAGAAAGGGAAGAAATTTACCCCGGAAAGCGGTGCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGAt                                                                                                                                     <  1:229876/143‑1 (MQ=255)  aGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATc                                                                                                                                    >  2:127513/1‑143 (MQ=255)   gAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCg                                                                                                                                   <  2:561185/143‑1 (MQ=255)         gaagaaATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTaaaa                                                                                                                             >  1:251437/1‑143 (MQ=255)          aagaaATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGCAGGCTTCCTGGTGGCCGAGATCGCTaaaaa                                                                                                                            >  1:542418/1‑143 (MQ=255)           agaaATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAACGAGCGTGCAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAg                                                                                                                           >  1:13825/1‑143 (MQ=255)                   aCCCCGGAGAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCg                                                                                                                   <  1:622284/143‑1 (MQ=255)                    tcccGGAAAGTGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGc                                                                                                                  <  1:17824/142‑1 (MQ=255)                      ccGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAACGCTTCCTGGTGGCTGAGCTCGCTAAAAAGCTGAACCGccg                                                                                                                >  2:517394/1‑141 (MQ=255)                         gAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTAt                                                                                                             >  1:311311/1‑143 (MQ=255)                           aaGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGACGAGATCGCTAAAAAGCTGAACCGCAGTATTa                                                                                                           >  2:570567/1‑143 (MQ=255)                              cGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGACGGCTTCCTGGTGGCCGATATCGCTTAAAAGCTGAACCGCATCATTaaaa                                                                                                        >  1:108351/1‑143 (MQ=255)                              cGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATCaaaa                                                                                                        >  2:708930/1‑143 (MQ=255)                                   ctcGCCTGGTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATc                                                                                                   <  1:661504/143‑1 (MQ=255)                                    tcGCCTGTTGGGAAAAATCAGGGCGGGTGTTTGTGGTGGGAACCCCCACACGCCAAAAGAGGGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCa                                                                                                  <  2:504712/143‑1 (MQ=255)                                     cGCCTGTTGGAAAGAAGCAGTGCTGGTGGTTACGGTGACAAGCGTCGCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGTAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAg                                                                                                 <  1:58423/143‑1 (MQ=255)                                         tGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGc                                                                                             <  1:295967/143‑1 (MQ=255)                                         tGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGc                                                                                             <  1:416254/143‑1 (MQ=255)                                             ggAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAACACCATCAGTAGCCaga                                                                                         >  1:181051/1‑143 (MQ=255)                                               aaaaaaTCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTAGTAAAACCATCAGTAGCCagaag                                                                                       >  2:480631/1‑143 (MQ=255)                                                aaaaaTCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCagaaga                                                                                      >  1:477575/1‑143 (MQ=255)                                                          gCTGGTGGTTACGGTGACCAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCgat                                                                            <  1:713226/143‑1 (MQ=255)                                                           cTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCgatg                                                                           >  2:521048/1‑143 (MQ=255)                                                           cTGGTGGTGACGGTGACAACCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCgatg                                                                           <  1:221957/143‑1 (MQ=255)                                                             gggggtTACGGTGGCAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCgatgat                                                                         <  2:386340/140‑1 (MQ=255)                                                                ggtTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGAtgatgat                                                                      >  2:420995/1‑142 (MQ=255)                                                                   taaggTGAAAAGCGTCTCTCGGCAAAAGAGAGGGAAGTACTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCt                                                                   <  1:40168/140‑1 (MQ=255)                                                                     cGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTACAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTgg                                                                 >  1:56530/1‑143 (MQ=255)                                                                       gTGACAAGCGTCTCTCGCCAAAATAGAGTGAACTTCTGCGCCTGGTTGGGGAAAGCGTCCTGGTTGCCGAGATCGCTAAAAAGCTGAACCGCATTATTAAAACCATCAGTAGCCAGAAGAAATCTGCTATGATGAAGCTGGgt                                                               <  2:44129/143‑1 (MQ=255)                                                                          aCAGGCGTCTCCCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTc                                                            <  1:397055/143‑1 (MQ=255)                                                                            aggCGTCTCTCTCCAAAAGAGAGTGAAGTGCTGCGCCTGTTTGCGGAAGGCGTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCga                                                          <  1:481540/141‑1 (MQ=255)                                                                            aaGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGCGGAAATCTGCGATGATGAAGCTGGGTGTCga                                                          >  1:89646/1‑143 (MQ=255)                                                                              gCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCgagc                                                        >  1:531717/1‑142 (MQ=255)                                                                                 gctcacGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACg                                                     <  2:583996/142‑1 (MQ=255)                                                                                    ctcGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAGAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAAAGata                                                  >  2:393422/1‑143 (MQ=255)                                                                                           aaaGAGCGTGAAGCTCTGCGCCTGTCTGCGGCAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGACGAAGCTGGGTGTCGAGAACGATATCGCCct                                           <  1:190094/143‑1 (MQ=255)                                                                                           aaaGAGAGTGAAGTTCTGCGCCTGTGTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCct                                           <  1:110262/143‑1 (MQ=255)                                                                                            cagagagTGAAGTTCGGCGCCTGTGTGCGGCAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTACAACCATCAGTAGCCAGAATATATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCctg                                          <  1:82024/142‑1 (MQ=255)                                                                                              gagagTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAACGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCccgct                                        >  2:346709/1‑143 (MQ=255)                                                                                                   tGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAGtt                                   >  1:672975/1‑143 (MQ=255)                                                                                                   tGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGAGGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAAtt                                   >  1:301710/1‑143 (MQ=255)                                                                                                     aaGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTAt                                 <  2:251437/143‑1 (MQ=255)                                                                                                      aGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTAtc                                <  1:415228/143‑1 (MQ=255)                                                                                                      aGTGCTGCGCCTGGTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTAtc                                <  1:657204/143‑1 (MQ=255)                                                                                                              ggcTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCa                        <  1:480631/141‑1 (MQ=255)                                                                                                              gcCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAACCAGAAGAAATCTGCGAAGATGAAACTGGGTGAAGAGAAAGATATCGAAATGCGGAATTAtgtctataca                        >  2:3906/1‑138 (MQ=255)                                                                                                                cTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGCAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGt                      <  1:527062/143‑1 (MQ=255)                                                                                                                cTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGt                      >  2:385132/1‑143 (MQ=255)                                                                                                                      gCGGAGTGCCTCCTGGTTGCCGAGATCGCTAAAACGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCtt                <  2:114097/143‑1 (MQ=255)                                                                                                                      gCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCCTCAGTGAccct                >  1:495728/1‑141 (MQ=255)                                                                                                                        ggAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCCCAGTTAACCCTCTTCAGTGACCTTaa              >  2:340783/1‑143 (MQ=255)                                                                                                                        ggAAGACTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGCATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGATGTCGAGAACGATAACGCGCTGCTGAATGAGAAATCTTCAATGACGTTaa              >  2:25195/1‑143 (MQ=255)                                                                                                                         gAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGATGAATAACACATATAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTCTCGAGAACGATATCGCCCTGCCGCATGATCTCTGTTCAGTGACCTTact             >  2:510753/1‑141 (MQ=255)                                                                                                                           aGGCGTCATGGTGGCCGAGGGCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGAGATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTc           <  1:170262/143‑1 (MQ=255)                                                                                                                             gCTTCCTGGTGGACGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCg         <  1:72179/143‑1 (MQ=255)                                                                                                                              tttCCTGGTGGCCGGGATAGGTAAAAAGCTTGACCGCAGTAATAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGTTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCgg        <  2:92746/142‑1 (MQ=255)                                                                                                                              cTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCgg        <  1:328121/143‑1 (MQ=255)                                                                                                                               ttCCTGGTGGCCTAGATCGCTAAAAAGCTGAACCGCAGTATTAAATCCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGc       <  2:328774/143‑1 (MQ=255)                                                                                                                               ttCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTCAGTCCGGc       <  2:477575/143‑1 (MQ=255)                                                                                                                               ttCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGc       <  1:702880/143‑1 (MQ=255)                                                                                                                               ttCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGc       <  2:569832/143‑1 (MQ=255)                                                                                                                                 ccTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAg     >  2:193368/1‑143 (MQ=255)                                                                                                                                  ggggTGGCCGAGATCGCTAAAAAGATAAAAAGCATTATTAATTCCTGCAGGAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGa    <  2:343814/141‑1 (MQ=255)                                                                                                                                    ggTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCGATGTAGCAGGATCTCTCTTCAGCGACCTTAAGTCAGGCAGATa  >  2:694695/1‑143 (MQ=255)                                                                                                                                        |                                                                                                                                           CAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGATA  >  NC_000913/2316546‑2316819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑