Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	84.2 / NA	28	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/14);  total (14/14)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132650‑2132926                                                                                                                                          |                                                                                                                                            tcgggcagagGGTAAACAGCTGATAGTTGTTGTTGGCGCGGTCGAAGTTGGCTGTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                        <  1:730959/137‑1 (MQ=255) tCTGGTAGAGGGTAAACAGCGTATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTATGCTTGCGGTAAAAGTGGAAGTACTCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                        <  2:333048/143‑1 (MQ=255)    gttAGATGGTAAACAGCTGATTTTTTTTGTTGGTGTGGTCGAATTTGTCTTTGTGCTTGCTGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                     <  2:220583/141‑1 (MQ=255)          gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctct                                                                                                                               >  1:276903/1‑143 (MQ=255)              aaaCAGCTGATATTGTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                           <  1:353045/143‑1 (MQ=255)                       atatTTTTTGCTGGCGCGGTTGGCTTTCCCTATATGCTTGCGGTAAAAGTTTAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg                                                                                                                  <  1:296943/143‑1 (MQ=255)                           tttttGCTGGCGCGGTCGAATTGGTCGTGGTGCTCGCGGTAAAAGTGGAAGGGCCCGGAGAATGTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTc                                                                                                              <  1:255407/143‑1 (MQ=255)                                 cTGGCGCGGTCGGGTGTGTCTGTGTGCTTGACGTAAGAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa                                                                                                        <  2:466628/143‑1 (MQ=255)                                     cgcgGTCGAATTTGTCTTTGTGCTTGCGGTATAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcc                                                                                                    <  1:403906/143‑1 (MQ=255)                                        ggTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTGCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacc                                                                                                 <  1:195218/143‑1 (MQ=255)                                         gTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca                                                                                                <  2:153572/143‑1 (MQ=255)                                            gAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCGGCGTCGCCTCTTCTTCTTTCCCCGGTTTGCCGTCCTccaccacca                                                                                             >  1:410018/1‑143 (MQ=255)                                                          gCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATg                                                                               <  2:249554/143‑1 (MQ=255)                                                                  tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGTAATc                                                                       >  1:23556/1‑143 (MQ=255)                                                                   aaaaGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTTGGTCACCACCCGCACGCACATGTCGAAATcc                                                                      >  2:562041/1‑143 (MQ=255)                                                                     aattgtaaGTACCCGGATAATTTTTTCGGTGACGAGGTGATCTGCATTGCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtg                                                                    <  1:273537/137‑1 (MQ=255)                                                                      aGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACGACCATCCGCAGGAAGATGTCGTACTCCtgt                                                                   >  1:623959/1‑143 (MQ=255)                                                                                   ggAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCACGAAGCTGTCGTAATCCTCTGCCCCCTTCAGtt                                                      >  1:462310/1‑143 (MQ=255)                                                                                       aaTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACGGTCCGCAGGAAGGTGTAGTAATCGTGTGCCGCTTGCAGGTCggg                                                  >  1:48295/1‑142 (MQ=255)                                                                                         ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGCTGTCGTAATCCTGTGCCGCTTTCAGGTCGGCAt                                                >  1:653201/1‑143 (MQ=255)                                                                                          tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                               >  1:707393/1‑143 (MQ=255)                                                                                              tCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGacc                                           >  1:747992/1‑141 (MQ=255)                                                                                                            cgcttcATTTCACGTTGCTTACTATTCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaa                             <  1:473207/138‑1 (MQ=255)                                                                                                                 cATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCAACACCATCCGCAGCAAGATGTCGTAATCCGGTGCCGCTTTCAGTTCGGTATCGAACACACACCCTTTGAAACgac                        >  1:750762/1‑141 (MQ=255)                                                                                                                     ttgccGTGCTTGCTAGTCAGGCTCTGCGTCGCCTCTTGTACTTTCCACGGTTCGCCGTACTCCACCATCATCCGCAGGAAGATGTCGGAATCCTGGGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGc                    <  2:312137/140‑1 (MQ=255)                                                                                                                             attgatATGCAGGCTCTGCGTCGCCTCTTCTACTGTCCACGGTTCGCCGTACTCCAACACCATCCGCAGGAAGATGTCGTCATCCTGTGCCGCGTTCAGTTCGGGCTCGAACAAACACTCTTTGAAACGCCATGAACAGGTac            >  2:437297/1‑142 (MQ=255)                                                                                                                                   atGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTGGAAACGCCATGCCCAGGTAAAGACCt      >  1:224460/1‑143 (MQ=255)                                                                                                                                       aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAACTGAtt  >  1:557706/1‑143 (MQ=255)                                                                                                                                          |                                                                                                                                            TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132650‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑