Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	1 (0.020)	88 (1.610)	56/284	0.0	98.8%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	1 (0.020)	85 (1.560)	52/284	0.1	98.8%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

AAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGA  >  NC_000913/2289976‑2290255                                                                                                                                          |                                                                                                                                               aattctcTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAAc                                                                                                                                           <  2:711256/140‑1 (MQ=18)     ctctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTg                                                                                                                                       >  1:98109/1‑143 (MQ=35)      tctTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGACTCCAGATGACAAACATGATCGCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGt                                                                                                                                      >  1:532771/1‑143 (MQ=255)       gttttCTGGTCTGGCGGCGCTTGCTGGTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTa                                                                                                                                     <  2:901741/142‑1 (MQ=255)               gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTGTGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGccaccac                                                                                                                             >  1:849217/1‑142 (MQ=255)                  tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAAc                                                                                                                          <  1:702072/143‑1 (MQ=255)                  tGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAAc                                                                                                                          <  2:861739/143‑1 (MQ=255)                   gACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGcaca                                                                                                                         >  1:697534/1‑143 (MQ=255)                    aCGGGGCTTCCTGCTGACTTGCCTGGCGGCGGAGGTGAGTGGATGACTCATGATGAGCCCTGGTCTATGGCTCCAGATGACAAGCATGATGTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAg                                                                                                                        <  2:237042/143‑1 (MQ=17)                           ttACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGc                                                                                                                 <  2:713333/143‑1 (MQ=255)                               tgctgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGaa                                                                                                             >  1:463253/1‑143 (MQ=255)                                   gAATCCCCTGTCGGCGCAGGTACGTTTATGACTCATGATGAACCCTCTTCTATGCCTCCAGATGACAAACATCATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGAc                                                                                                         <  2:147745/143‑1 (MQ=18)                                      tgcACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCATGCTCTATTGCTCCAGATGACAAACATAATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACaaa                                                                                                      <  1:269873/141‑1 (MQ=255)                                        cACTGTCTGCGAAGGTAAGTTGATGACTCATGATGAGCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCAGATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAaga                                                                                                    <  2:905291/143‑1 (MQ=255)                                         aCTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGCAACGACAAagag                                                                                                   >  2:715079/1‑143 (MQ=255)                                           tGTCGGCGAAGGTCAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGACGTAGCCACCAGAACAGATATTGCGCAACGCCACAGAGaa                                                                                                 >  2:928846/1‑143 (MQ=17)                                             tCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAAc                                                                                               <  1:69280/143‑1 (MQ=255)                                                  gAAGGTAAGTTGATGACTAATGATGAAACCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTAGCACCGTCCATAACGCTGTAGCCACCAGAACAAAAATTGCAGAACGACAAAGAGAAACAGAAc                                                                                          >  2:266158/1‑143 (MQ=21)                                                         aGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACCAAGCGAAACAGAACCAGAGTg                                                                                   >  2:691504/1‑143 (MQ=255)                                                           ttGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGAt                                                                                 <  1:113919/143‑1 (MQ=255)                                                             gatgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCCGATTGATGc                                                                               >  1:418297/1‑143 (MQ=255)                                                               tgaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGAACTCATATCAGGGACTTGTTCGCACCCTCCATAACGCTGTAGCCACCAGAACAGATATTGCCGAACGACAAAGAGAAACAGAACCGAATTGATggcg                                                                             >  2:662105/1‑140 (MQ=25)                                                                gaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAtt                                                                            >  1:925756/1‑143 (MQ=255)                                                                gaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCAtt                                                                            <  1:910065/143‑1 (MQ=255)                                                                gaCTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTGCCATAACGCTGTAGCCACCAGAACAGGTCTTGCGGAACGGCCACGAGAAACAGAAACAGATTGCTGCAtg                                                                            >  2:20991/1‑142 (MQ=21)                                                                   tCATGATGAACCCTGTACTATGCCTCGAGATGAAAAGAATGATCTCATATCCGGCAGTGCTCCGCACCTTCGATATTCTATTAGCAACGACAATGGATATTCCGGTACGACAAAGTGAAACAGAACCAGATTGATGCATTGat                                                                         >  2:438632/1‑142 (MQ=255)                                                                     atgatgAACCCTGTTCTAAGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAAAGCTGTAGCCACCAGAACAGATAATGCGGAAAGACAAAGAGAAAAAGAACCAGCATGAAGCaaagaacc                                                                       >  2:310462/1‑136 (MQ=21)                                                                         tgaACCCTGTTCAATCGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCCCCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATTCATCGAGCCTTCa                                                                   >  2:305953/1‑143 (MQ=25)                                                                            aCCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACAAGCTGGAAGCAGTGCGCTCGCATca                                                                >  1:978776/1‑142 (MQ=25)                                                                             cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGCTTGCTGCTTTGAGCTTTCATCCt                                                               >  1:28378/1‑143 (MQ=255)                                                                             cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCt                                                               >  1:383546/1‑143 (MQ=255)                                                                              ccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCCGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGCACCAGATTGATGCATTGAGCTTTCATCCTa                                                              >  1:919031/1‑143 (MQ=255)                                                                                  ttCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGCACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGCGCTTTCATCCTATGaa                                                          >  1:165510/1‑143 (MQ=255)                                                                                       tGGCTCCCGATGCCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAaattaa                                                     <  2:138808/143‑1 (MQ=255)                                                                                            tcAGATGACAAACATGATCTCAGATCAGTGACTTGTTCGCACCTTCTATAACGCTGTAGCGACCAGAACCGATATTGCGGAAGGCCAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATtgcttgct                                                <  2:173445/142‑1 (MQ=37)                                                                                            ccAGATGACAAACATGATCTCATAGCAGGTACTTGAACTCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCt                                                <  1:671762/143‑1 (MQ=255)                                                                                             cAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTg                                               <  2:572897/143‑1 (MQ=255)                                                                                                   aCAAACACGATCTCATATCAGGGACTTGCTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATGGCTGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTaaaa                                         <  2:595854/143‑1 (MQ=255)                                                                                                    cacaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAg                                        <  1:311415/140‑1 (MQ=255)                                                                                                     aaaCATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGc                                       >  1:350025/1‑143 (MQ=255)                                                                                                        cATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCAtt                                    <  1:258035/143‑1 (MQ=255)                                                                                                          tGATCTCATATCAGTGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGCACAGATATTGCGGAACGACAAACAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTgg                                  <  2:493466/143‑1 (MQ=255)                                                                                                           gATCTCATATCAGGGACTTGTGCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCCTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTggg                                 <  1:489054/143‑1 (MQ=255)                                                                                                               tcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTTGGTACa                             >  1:513952/1‑143 (MQ=255)                                                                                                               tcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACa                             >  1:325450/1‑143 (MQ=255)                                                                                                                 atatCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGa                           >  1:652344/1‑143 (MQ=255)                                                                                                                    tCAGCGACTCGTTCGCTCCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGACCCAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGaaaa                        <  1:932011/143‑1 (MQ=255)                                                                                                                        ggACGTGGTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATAcc                    <  1:595526/143‑1 (MQ=255)                                                                                                                          aCTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAAATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCa                  >  2:183673/1‑143 (MQ=255)                                                                                                                            ttGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATa                >  1:317973/1‑143 (MQ=255)                                                                                                                             cgTTCGCACGTTCCATAACGATGTAGCCAACAGAACAGGTATGGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTGTGGAATTAATAACTATTAAAAGCATTGGGTACAGAAAATACCCATAg               <  2:441424/142‑1 (MQ=255)                                                                                                                                          cATAACGCTGTAGCCACCAGAACAGATATTGCGGTACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGa  <  1:297731/143‑1 (MQ=255)                                                                                                                                          cATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGa  <  1:797861/143‑1 (MQ=255)                                                                                                                                          |                                                                                                                                               AAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGA  >  NC_000913/2289976‑2290255

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.