Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	98.6%	197.0 / NA	71	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (35/34);  total (36/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132647‑2132925                                                                                                                                             |                                                                                                                                           ggATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTc                                                                                                                                          <  1:1197140/143‑1 (MQ=255)   aTCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTg                                                                                                                                        >  2:555230/1‑143 (MQ=255)    tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGTTTGTTATGCAGGCTCTGc                                                                                                                                       >  1:84725/1‑143 (MQ=255)     cTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGGGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTggg                                                                                                                                      >  2:481958/1‑141 (MQ=255)       ggTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                    >  1:861624/1‑143 (MQ=255)        gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                   >  2:996851/1‑143 (MQ=255)        gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                   >  2:540334/1‑143 (MQ=255)         tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGATTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                  <  1:1031297/143‑1 (MQ=255)         tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAGTTTGTGTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGCGCATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTCATATGCAGGCCCTGCGTCGc                                                                                                                                  >  1:1253146/1‑143 (MQ=255)         gagagGGTAAAGAGCTGATCTTTTGTGCTGGCGCGGGCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                  <  2:1209350/142‑1 (MQ=255)          agagGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGcc                                                                                                                                 >  1:772326/1‑143 (MQ=255)                 aaaCAGCTGATCTTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTACAAGTGGCCGTACCCGGAGACTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCCCCccttctc                                                                                                                          >  2:125243/1‑142 (MQ=255)                  atcAGCTGTTAGTTTTTGCTGGCGGTGTTGAATTTGTCTTTGTGCTTGCGGTGAAAGTGGAAGTACCTGGATAATTTTTTCGGTGACGAGCTGATCTGCATTTCACCGTGATTGTTATGCAGGCTCTGCGTCGCCTCTTCTAc                                                                                                                         <  2:1246717/141‑1 (MQ=255)                  aaCAGCTGATATTTTTTGCTGGCGCAGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTAc                                                                                                                         <  1:217394/143‑1 (MQ=255)                        tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                   >  2:1317532/1‑143 (MQ=255)                        tGATATTTTCTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                   <  2:566698/143‑1 (MQ=255)                           tatTGTTTGCTGGCGCGGTCGGATTTGTCTTTGTGCTTGCAGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACgg                                                                                                                <  2:566867/143‑1 (MQ=255)                             ttttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                              <  2:469697/143‑1 (MQ=255)                             tgttttGCTGGCGCGGTCGAATTTGTCGCTTTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGGGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGtt                                                                                                              <  2:985364/141‑1 (MQ=255)                              tttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTACAAGTGGAAGTAACCGGAGAATTTTTTCGGTGATGAGGTGATCTGCATTTCAGCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGttt                                                                                                             >  2:257218/1‑142 (MQ=255)                                         gcgGTCGCATTTGTCTTTGTGCTTGCGGGAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcca                                                                                                  <  2:1295810/143‑1 (MQ=255)                                          cgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac                                                                                                 <  2:235461/143‑1 (MQ=255)                                            gTCGAATTTGTCTGTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGACCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca                                                                                               <  1:346665/143‑1 (MQ=255)                                              cGAATTTGGCTTTGTGCTTGCGGTCGCAGTGGCAGTAGCTGGAGAATTTTTTCGGTGGCGAGGTGGTCTGGATTTCACCGTGATTGATATGCAGGCTCTGCGACGCCTCTTCTACTTTCCACGGTTCGCCGTACTccaccacc                                                                                             <  2:269648/143‑1 (MQ=255)                                                  tttGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGCCGCCTCTGCGACTTTCCACGGTTCGCCGTGCTccaccaccagca                                                                                         >  1:110474/1‑140 (MQ=255)                                                    tGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGc                                                                                       <  1:540334/143‑1 (MQ=255)                                                    tGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGc                                                                                       <  1:996851/143‑1 (MQ=255)                                                     gTCTTTGTGCTTGCGGTAAAAGTGGGAGTACCCGGAGAATTTGTTCGGTGACGAGGTGGTCTGCATTTCGCCACCAGCTAGATGGAGGCTCTGCGTTGCCCCCTCTACCTCCGACGGGTAGCCGTACCCCTCCACCCTCCGcc                                                                                      >  2:277038/1‑142 (MQ=255)                                                        tttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCAACCACCATCAGCAGGa                                                                                   >  1:1189274/1‑143 (MQ=255)                                                         gtgtgATTGCGGGAAAAGCGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                                  <  2:1087830/142‑1 (MQ=255)                                                               gtGCGGAAAAAGTGGAAGCACCCGGAGAAGTTGTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGCACTCCACCACCATCCGCAGGAAGATGTc                                                                            <  1:1263371/142‑1 (MQ=255)                                                                tGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCg                                                                           <  1:1146305/143‑1 (MQ=255)                                                                   ggcaaaaGTGGATGTACCCGGAGATTTTTTTCGGTGACGAGGTGATCTGCGTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTaa                                                                        <  2:28471/140‑1 (MQ=255)                                                                    gTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGATGGCGTaag                                                                       >  2:307175/1‑142 (MQ=255)                                                                     tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGCGATTGATAGGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGCACTCCACCACCATCCGCAAGAAGATATCGCAATc                                                                      >  2:301215/1‑143 (MQ=255)                                                                      caaaGTGGAAGTACGCGGAGAATTTTTTCGGTAACGTTCTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATcc                                                                     <  2:270714/142‑1 (MQ=255)                                                                        aaGTGGAAGTACCCGGAGAATTTTTTCGGTGACAAGGCGGTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCCTCACCTCTTCTACATGCCACGGTTCGCCGTACTCCGCCACCATCCCCCCGAAGACGTCGTACTccct                                                                   >  2:23593/1‑141 (MQ=255)                                                                              tagcccccGGAGCATTTTGTCGGTGATGAGGTGCTCTGCATGTCACCGTGATTGAGATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGc                                                             <  2:551103/138‑1 (MQ=255)                                                                              aaGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTGCACTCTTCTCCTTTCCACGGCTCGCCGTACTCCACCGCCATCCGCACGAAGGTGTCGTCATGCTGTccccc                                                             >  2:941375/1‑141 (MQ=255)                                                                              aaGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGc                                                             <  1:335956/143‑1 (MQ=255)                                                                                   cccGGCGATGTTTTTCGGTGTGGTGGTGATCTGCAGTGTACCGTGATTGATATGCGGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGTCGCTTTCa                                                        <  2:1223261/143‑1 (MQ=255)                                                                                   cccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGGGCCGCCTCTTCTACTTTCCAAGGTTCGCCGTACTCCACCACCATCAGCAGGACGATGTCGTACGTCTGTGCCGCTTGCa                                                        >  2:205714/1‑143 (MQ=255)                                                                                    ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCCCAGGACCCTGTCGCACTCCTGTGCCCCtctccg                                                       >  2:828486/1‑141 (MQ=255)                                                                                       gtgtaTTCTTTCGGTGTCGGGGTGATCTGCATTTCACCGGGATTGATATGCAGGCTCTGCGTCGCCCCTTCTACCTTCCACGTTTCGTCGTACTCCACCACCTTCCGTTGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc                                                    <  2:657837/139‑1 (MQ=255)                                                                                       aagaATTTTTTCGGTGACGAGGTGACCGGCAGTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc                                                    <  1:416610/142‑1 (MQ=255)                                                                                         gaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCGGCGTCGACTCCTCTACTGTCTACGGTTCGACGTTGTGCACCACCATCGGCAGGAAGATGTCGTGAGCCTTGGCCGCTGTCAgggcgg                                                  >  2:155828/1‑138 (MQ=255)                                                                                            ttttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt                                               >  1:753128/1‑143 (MQ=255)                                                                                               tttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCCTACTCCACCACCGTCCGCAGGAAGGTGGCGTACTCCTGTGCCGCTTTCAGGTCGGTATCGa                                            >  2:21462/1‑143 (MQ=255)                                                                                                     tGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTGCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCCTTCAGTTCGGGACCCaacaaac                                      >  2:1251881/1‑143 (MQ=255)                                                                                                         gagaTGATCTGCATTTCACCGTGATTGATATGCAGGCTCAGCGTCGCATCTTCTACTATCCACGGTTCAACCTACTCCACCACCATCCGCAAGAAGATGACGTACTCCTGTACCGCATACCGTTCCGTATCGAACAAACAatc                                  >  2:890799/1‑143 (MQ=255)                                                                                                           ggTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCtt                                <  1:812131/143‑1 (MQ=255)                                                                                                            gtgcccTGCTTCTCCCCCTGATCGATATGCGTCCTCTTCGTCGCCTCTTCTTCTTTCCACGTTTCGCCGGACGCCACCACCACCATCAGGAAGATGTCGAAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt                               <  2:908284/138‑1 (MQ=255)                                                                                                             tGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTACTCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTg                              >  2:1076124/1‑143 (MQ=255)                                                                                                              gATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa                             <  1:635482/143‑1 (MQ=255)                                                                                                              gATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGa                             >  1:1124212/1‑143 (MQ=255)                                                                                                                tCTGCATTTCACCGGGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaaa                           <  1:224579/143‑1 (MQ=255)                                                                                                                  tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACg                         >  1:400998/1‑143 (MQ=255)                                                                                                                  tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGATTCGCCGTACTCCACCACCCTCCGCCGGAAGATGTCGTAAACCTGTGCCGCTTTCAGCTCGGTATCGAACAAACACTCGTTgcatcg                         >  2:1145915/1‑138 (MQ=255)                                                                                                                   aaaTTTCTCCGTGATTGATATGCAGGCTCTGCGACGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTATCAGTTCAGAATCGAACAAACAAACTTTGAAAcac                        >  2:31277/3‑141 (MQ=255)                                                                                                                      tttCGCCGTGATGGTTATGCAGGTTCGGCGGCGCCGCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATGCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCAt                     <  2:421701/143‑1 (MQ=255)                                                                                                                      tttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACGCCCAGCAGCAGGCAGATGTCGTAATCCTGTGCCGCGTTCAGCTCGGTATCGACCAAACACCCTTTGGCACGCCAt                     >  1:572868/1‑143 (MQ=255)                                                                                                                            cGTGATTGACATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAg               <  1:733823/143‑1 (MQ=255)                                                                                                                              tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGt             >  2:1120223/1‑143 (MQ=255)                                                                                                                                attgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACCCTCTTTGAAACGCCAGGCCCAGGCaa           >  2:119876/1‑143 (MQ=255)                                                                                                                                 ttgctATGCAGGCTCGGCGCCGCCTCTTCCACTTGCCACGGGCCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa          <  1:248575/143‑1 (MQ=255)                                                                                                                                  tgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAg         >  1:906457/1‑143 (MQ=255)                                                                                                                                   gatATGCAGGCTCTGCGTCGCCTCTTCTAGTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGa        <  2:127695/143‑1 (MQ=255)                                                                                                                                    atatGCAGGCTCTGCGTCGCCTCTTCTGCTTTCCCCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGAc       <  2:97669/143‑1 (MQ=255)                                                                                                                                      gtGCAGGCTCTGCGTCTCCTCTTCTACGTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCt     <  2:772326/142‑1 (MQ=255)                                                                                                                                         cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAAAGCCATGCCCAGGTAAAGACCTGGt  >  1:707292/1‑143 (MQ=255)                                                                                                                                         cAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATAGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGt  >  1:515821/1‑143 (MQ=255)                                                                                                                                             |                                                                                                                                           GGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGT  >  NC_000913/2132647‑2132925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑