Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 704,083 C→A A47E (GCG→GAG)  nagE → N‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913704,0830CA93.2% 110.3 / NA 45A47E (GCG→GAG) nagEN‑acetyl glucosamine specific PTS enzyme IIC, IIB, and IIA components
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base A (21/20);  total (24/21)
Fisher's exact test for biased strand distribution p-value = 2.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGTAGGTTACTTTGTGTTAACCAAAGCGATGGTGACCAT  >  NC_000913/703942‑704212
                                                                                                                                             |                                                                                                                                 
aGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGAGACTCGGTCAGCCAGATTTACTTACCGTTGCGTTTATTGCCGAGCCGGGCGGTTCg                                                                                                                                  >  2:866044/1‑143 (MQ=255)
    gAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCCCTGTTGCTGCGATTCGGTCAGCCAGCTTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGAttt                                                                                                                              >  1:618856/1‑143 (MQ=255)
     aaTGTTTTGGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGACGCGGTCCGCCGGATTTTACAAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGAtttt                                                                                                                             <  2:741705/143‑1 (MQ=255)
            tAGGTTTTTTCCAGCGACGCGGGAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAAc                                                                                                                      <  1:879497/143‑1 (MQ=255)
                   tttCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCAt                                                                                                               <  1:761482/143‑1 (MQ=255)
                         gCGACGCGGTAGGGCGTTACCGCCCCCGATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTGGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCt                                                                                                         <  1:497263/143‑1 (MQ=255)
                           gACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTc                                                                                                       <  2:260687/143‑1 (MQ=255)
                             cTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATACCCTCGCCTTAATCTTCGc                                                                                                     >  1:349584/1‑143 (MQ=255)
                              tCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGCTTTACTTAACGTTGCGTTTATTGCCCCGCCGGGCGGTGCGGTTCTTGATAACCTCGCCTTCATCTTCgac                                                                                                    >  1:553494/1‑141 (MQ=255)
                                ggTAGGGCGTTACAGCTCCCTATAGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTGACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTGTTGATAAAGTCGCATTAATCTTCGccct                                                                                                  >  2:693860/1‑141 (MQ=255)
                                     ggCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGgtg                                                                                             >  1:524064/1‑143 (MQ=255)
                                       cGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTGACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCAGtgtg                                                                                           >  2:805128/1‑143 (MQ=255)
                                         tgaCAGCGCCCTGTCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGc                                                                                         <  1:150279/141‑1 (MQ=255)
                                          tACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCa                                                                                        >  1:780324/1‑143 (MQ=255)
                                            cAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATc                                                                                      <  2:518718/143‑1 (MQ=255)
                                            cAGCTCCCTAGCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATc                                                                                      <  2:254460/143‑1 (MQ=255)
                                             aGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACTTCGCATTAATCTTCGCCACCGGCGTGGCATcc                                                                                     >  2:468012/1‑143 (MQ=255)
                                              gCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAACCTTCGCCCTCGGTGTGGCTTCCa                                                                                    >  1:248898/1‑143 (MQ=255)
                                               cTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACATTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAg                                                                                   <  1:552034/143‑1 (MQ=255)
                                                    tATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGt                                                                              >  1:737903/1‑143 (MQ=255)
                                                         cgctgctgcCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGaaa                                                                         <  2:530571/140‑1 (MQ=255)
                                                         cgGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGaaa                                                                         <  1:264678/143‑1 (MQ=255)
                                                         cgGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGaaa                                                                         <  2:770689/143‑1 (MQ=255)
                                                         cgGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCAGTAATCTGCGCCATCGGTGTGGCATCCAGCTGGTCGaaa                                                                         <  2:600829/143‑1 (MQ=255)
                                                            tgctgcCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGAc                                                                      <  1:820474/143‑1 (MQ=255)
                                                                         ggcACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTAGACATGCGTGTGTCGGCCAGCTGGTCGAAAGACAGCGCTGGTgcgg                                                         >  1:855306/1‑143 (MQ=255)
                                                                         ggcACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTGTTGATAACCTCGCATTAATCCTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGcggcgg                                                         >  2:395640/1‑143 (MQ=255)
                                                                                   ctgcGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGc                                               >  1:225350/1‑143 (MQ=255)
                                                                                     gcGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCACAGGCGGGTGGTGAGATTTTTGATAACCTCGCAGTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGACAGACAGCGCCGGTGCGGGGGCGCTGGCgg                                             >  1:905691/1‑143 (MQ=255)
                                                                                      cGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCggg                                            >  1:178380/1‑143 (MQ=255)
                                                                                         tgcGGTCAGCCAGAGTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGc                                         <  2:618856/141‑1 (MQ=255)
                                                                                            ggTGAGCCGTATTTGCTCTACGTTGCGTTTAGTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTGCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGt                                      <  2:895464/143‑1 (MQ=255)
                                                                                             gTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGTa                                     >  1:534908/1‑143 (MQ=255)
                                                                                                    aGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTCTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGCGCGCGGTACGTTACt                              >  2:339506/1‑143 (MQ=255)
                                                                                                       tttACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGTAGGTTACTTtg                           >  1:680042/1‑143 (MQ=255)
                                                                                                        ttccTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGTGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCAGCCAGCTGGTCGAGAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGTAGGTTACTTtgt                          <  2:53644/140‑1 (MQ=255)
                                                                                                         tACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGTAGGTTACTTtgtg                         >  1:555657/1‑143 (MQ=255)
                                                                                                            ttATCGTTGCCTTGATGGCCCAGGCGGGCTGTGATATTTTTGATCGCCTCGCATTACTCTTCGCCATCGGTGTGGCATCCAGCTGGGCGAAAGACAGCGCTGGGGCGGCGGCGCTGGCGTGTGCGGTAGGTTACTTTGTGTTa                      <  2:831786/143‑1 (MQ=255)
                                                                                                            ttAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGTAGGTTACTTTGTGTTa                      >  1:216509/1‑143 (MQ=255)
                                                                                                             tagcGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTCTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGACAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGTATGTTACTTTGTGTTaa                     <  1:866044/140‑1 (MQ=255)
                                                                                                             tAACGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGTAGGTTACTTGGTGTTaa                     >  1:534797/1‑143 (MQ=255)
                                                                                                              aaCGTTGCGTTTATTGCCCAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGTAGGTTACTTTGTGTTAAc                    >  1:122633/1‑143 (MQ=255)
                                                                                                                        tttttGCCCAGGCGGGCGGTCAGATTGTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGTAGGTTACTTTGTGTTAACCAAAGCGATg          <  2:820903/140‑1 (MQ=255)
                                                                                                                               ccAGGCGGGCGGTGAGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGTAGGTTACTTTGTGTTAACCAAAGCGATGGTGACCa   >  1:514003/1‑143 (MQ=255)
                                                                                                                                cAGGCGGGCGGTTAGATTTGTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGTAGGTTACTTTGTGTTAACCAAAGCGATGGTGACCAt  <  2:720967/143‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                 
AGATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCACTGTTGCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCATTAATCTTCGCCATCGGTGTGGCATCCAGCTGGTCGAAAGACAGCGCTGGTGCGGCGGCGCTGGCGGGTGCGGTAGGTTACTTTGTGTTAACCAAAGCGATGGTGACCAT  >  NC_000913/703942‑704212

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑