Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	1,299,499	Δ1,199 bp	intergenic (+254/‑485)	ychE → / → oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	1299499	1300697–1299500	2–1199	46 [0]	[0] 46	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1299498	0 (0.000)	44 (0.980)	39/276	0.1	100%	intergenic (+253/‑1684)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
	?	NC_000913	1300698 =	0 (0.000)					intergenic (+1453/‑484)	ychE/oppA	UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein

GATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAC  >  NC_000913/1300556‑1300837                                                                                                                                               |                                                                                                                                            aattacagttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaa                                                                                                                                             <  1:549579‑M2/1‑1 (MQ=255)       agttagtcataagcatttccttagctgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaataataa                                                                                                                                       <  1:22877‑M2/7‑1 (MQ=255)         ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcctaatcctgggcttgctgacgaataattgaactgatattattaattacactccctctggtagaagaaagtgctaaATAATAATc                                                                                                                                     >  2:89687‑M2/135‑143 (MQ=255)         ttagttataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATc                                                                                                                                     >  1:621109‑M2/135‑143 (MQ=255)               ataaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGt                                                                                                                               >  1:516763‑M2/129‑143 (MQ=255)                taaggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGtt                                                                                                                              >  1:702938‑M2/128‑143 (MQ=255)                   ggatttccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTaaa                                                                                                                           >  2:189131‑M2/125‑143 (MQ=255)                    gatttccttaactgcttctcctcaccatcatgttattttcgccacatcctaatcctgggcttgctgacgaataattgaaatgatattattaattccactgcctttggtataggacagtgctaaATAATAATCAATTGTTAAat                                                                                                                          >  2:587571‑M2/124‑143 (MQ=255)                        tccttaactgcttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAattatt                                                                                                                      <  1:394923‑M2/24‑1 (MQ=255)                          cttaactggttctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATtgt                                                                                                                    <  2:250222‑M2/26‑1 (MQ=255)                                     ctcctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCacta                                                                                                         <  1:734359‑M2/37‑1 (MQ=255)                                        ctcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTg                                                                                                      >  2:311659‑M2/104‑143 (MQ=255)                                         tcaccatcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCACTTGTTAAATTATTGTGCATTTCACTACTgg                                                                                                     >  2:250722‑M2/103‑143 (MQ=255)                                            ccctcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAAc                                                                                                  <  1:59978‑M2/44‑1 (MQ=255)                                            ccatcatgttattttcgccacatcataatccttggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagttctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAAc                                                                                                  <  2:716987‑M2/44‑1 (MQ=255)                                            ccatcatgttattttcgccacatcataatcctgtgcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAAc                                                                                                  <  1:625546‑M2/44‑1 (MQ=255)                                             catcatgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctctggtagaggaaagtgctaaATAATAATCAATTGTTCAAATATTGCGTATCACACTACtgaaatt                                                                                                 >  2:310294‑M2/99‑141 (MQ=255)                                               tcatggtattttcgccacatgataatcctgggcttgctgaagaataagtgaaatgatattattaattccactgccgttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGt                                                                                               <  2:401044‑M2/47‑1 (MQ=255)                                                 ttgttattttcgcaacttcataatcctgtgcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTaa                                                                                             <  2:636037‑M2/49‑1 (MQ=255)                                                  tgttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTgtact                                                                                            >  1:411574‑M2/94‑141 (MQ=255)                                                    ttattttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCa                                                                                          >  1:638296‑M2/92‑143 (MQ=255)                                                        gttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaa                                                                                      <  1:772420‑M2/56‑1 (MQ=255)                                                         ttcgccacatcataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGaaaa                                                                                     <  2:179369‑M2/57‑1 (MQ=255)                                                                    ataatcctgggcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCGCTACTGGAACTGTAATCAGAAAAGATAGACATGc                                                                          >  1:238953‑M2/76‑143 (MQ=255)                                                                        tcctgggcttgctgaagaataattgaaatgatattattaattccactgcctatagaagaagaaagtgataaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAg                                                                      >  1:645672‑M2/72‑143 (MQ=255)                                                                              gcttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtc                                                                >  1:738819‑M2/66‑143 (MQ=255)                                                                                ttgctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAAtctc                                                              >  1:120290‑M2/64‑143 (MQ=255)                                                                                  gctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTa                                                            <  1:474592‑M2/82‑1 (MQ=255)                                                                                  gctgaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTa                                                            <  1:209138‑M2/82‑1 (MQ=255)                                                                                     gaagaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAttt                                                         >  1:432376‑M2/59‑143 (MQ=255)                                                                                        gaataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgat                                                      <  1:267154‑M2/88‑1 (MQ=255)                                                                                         aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTAtttgatc                                                     >  1:778528‑M2/55‑142 (MQ=255)                                                                                         aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                     <  1:347397‑M2/89‑1 (MQ=255)                                                                                         aataattgaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATttgatt                                                     >  1:93892‑M2/55‑143 (MQ=255)                                                                                                gaaatgatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGattgaattga                                              >  1:187379‑M2/48‑143 (MQ=255)                                                                                                 caatagtatgatcaattccactgcctttgctagaggaaagttctaaATAATAATCAATTGTTAAATTATTGTGCATTTTACTACTCTAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGAttgaattgaa                                             <  2:232060‑M2/97‑1 (MQ=255)                                                                                                     gatattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGAt                                         >  2:442268‑M2/43‑143 (MQ=255)                                                                                                      atattattaattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGatc                                        >  1:289798‑M2/42‑142 (MQ=255)                                                                                                              aattccactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTaa                                <  1:189131‑M2/110‑1 (MQ=255)                                                                                                               attacactgcctttggtagaggaaagtgctaaATAATAATCAAGTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAg                               <  2:768606‑M2/111‑1 (MQ=255)                                                                                                                   cactgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCAt                           <  2:621109‑M2/115‑1 (MQ=255)                                                                                                                      tgcctttggtagaggaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAACGATGTTTGTTAAGGCATGGa                        >  1:725650‑M2/26‑143 (MQ=255)                                                                                                                                    gaaagtgctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGAt          >  2:96978‑M2/12‑143 (MQ=255)                                                                                                                                         tgctaaATAATAATCAATTGTTAAATGATTGTGCATTTCACTACTGGAACGGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGa     <  1:89042‑M2/137‑1 (MQ=255)                                                                                                                                           ctaaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATa   <  2:187379‑M2/139‑1 (MQ=255)                                                                                                                                            taaATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAc  >  1:773675‑M2/4‑143 (MQ=255)                                                                                                                                               |                                                                                                                                            GATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATAATAATCAATTGTTAAATTATTGTGCATTTCACTACTGGAACTGTAATCAGAAAAGATAGACATGCTTAGCCAATCTCTATTTGATTGAATTGAAAGATGTTTGTTAAGGCATGGATGCAAGCTATAGATTCTGATAC  >  NC_000913/1300556‑1300837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑