Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,316,679 A→G T169A (ACC→GCC)  rcsB → response regulator in two‑component regulatory system with RcsC and YojN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,316,6790AG98.4% 171.4 / NA 61T169A (ACC→GCC) rcsBresponse regulator in two‑component regulatory system with RcsC and YojN
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (28/32);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

CAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGAT  >  NC_000913/2316544‑2316816
                                                                                                                                        |                                                                                                                                         
ccgAAGGGGAAGAGATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACTGTGACATGCGTCTCTCGCCAAAAGGGAGTGAAGTTCTGCCAATGTTTGGTGATCGCTTCCTGGTGGCCGAGAt                                                                                                                                    <  2:488940/141‑1 (MQ=255)
  gagaGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCg                                                                                                                                  <  2:540168/140‑1 (MQ=255)
  gAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCg                                                                                                                                  >  1:745776/1‑143 (MQ=255)
  gAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCg                                                                                                                                  <  1:301723/143‑1 (MQ=255)
         aagaaATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTaaaaa                                                                                                                           <  2:19844/143‑1 (MQ=255)
         aagaaAGTTACCCTGGAGAGCGGTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTaaaaa                                                                                                                           <  2:107830/143‑1 (MQ=255)
           gaaATTTACGTCGGATAGCGTTGTTCGCCTGTTGGAATAGTTCAGTGCTGGTGGTTTCGGTGACAAGCGTCTCTCTCCATAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGc                                                                                                                         <  2:277125/143‑1 (MQ=255)
                 tACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAAcc                                                                                                                   >  1:514704/1‑143 (MQ=255)
                      cGGAAGGCGTTTCTCGGCGGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGt                                                                                                              <  2:444497/143‑1 (MQ=255)
                      cGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTGAAAGGCTGAAGCGCAGt                                                                                                              >  2:699636/1‑143 (MQ=255)
                        gAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTAt                                                                                                            <  1:426378/143‑1 (MQ=255)
                            gCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTaac                                                                                                        >  2:54731/1‑142 (MQ=255)
                            gCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGGCACTCGCCAAAAGCGAGTGAAGTTCTGCGCCTGTATGACGGAAGCTTCCTGGTGGCCGAGATCGCCAACCAGCTGCACAGCAGCATTaca                                                                                                        >  2:525143/1‑141 (MQ=255)
                               tttCTCGCTTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAAcc                                                                                                     <  2:38228/143‑1 (MQ=255)
                                 tctcGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCAt                                                                                                   >  1:576113/1‑143 (MQ=255)
                                   tcGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAGAGCTGAACCGCAGTATTAAAACCATCa                                                                                                 >  2:674227/1‑143 (MQ=255)
                                   tcGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGAGCGCTAAAAAGCTGAACCGCAGTATTAAGACCATCa                                                                                                 >  1:536397/1‑143 (MQ=255)
                                          ttGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCa                                                                                          >  2:254305/1‑143 (MQ=255)
                                             gAAATAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCagaa                                                                                       <  1:587530/143‑1 (MQ=255)
                                              aaaaaaTCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCagaag                                                                                      >  2:523005/1‑143 (MQ=255)
                                              aaaaaaTCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCAGGAGACAGCGTTCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCagaag                                                                                      >  1:181928/1‑143 (MQ=255)
                                               aaaaaTCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTACAACCATCTGTAGcccgccca                                                                                     >  1:169129/1‑137 (MQ=255)
                                                    tCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCGGCGCCGGTGTGCGGAAGGCTTCCTGGTGGCCGAGAGCGCTAAAAAGCTGAGCCGGCGACTTAGAACCATCAGTAGCCAGAAGACAtgt                                                                                >  2:614465/1‑141 (MQ=255)
                                                     tattGCTGGTGGTTACGGTTACAAGCGTCTCGCGCAATAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCGTCTTGGCGGCCGAGATCGCTAAAAAGCTGACCCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTg                                                                               <  2:48342/140‑1 (MQ=255)
                                                        tGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCga                                                                            >  2:673835/1‑143 (MQ=255)
                                                        ggcgcgtggtTACGGTGACTAGAGTCGCGCGCCGAAAGAGAGTGCAGTGCTGCGTCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCga                                                                            <  2:61242/138‑1 (MQ=255)
                                                         gCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGGGATTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCGAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCgat                                                                           <  2:30250/143‑1 (MQ=255)
                                                            ggtggtTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCCGTAGCCAGAAGAACTCTGCgatgat                                                                        >  2:132763/1‑143 (MQ=255)
                                                            ggtggtTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCgctgat                                                                        >  1:658428/1‑143 (MQ=255)
                                                               ggtTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGaa                                                                     <  1:673835/143‑1 (MQ=255)
                                                               gggtACGGTGGCAGTCGTCTCTCGCCAAGAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGaa                                                                     <  2:745776/140‑1 (MQ=255)
                                                                 ttACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGGATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGc                                                                   >  1:287592/1‑143 (MQ=255)
                                                                    gggTGACGAGCGGCTCTCGCCACAAGAGAGTGAGGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTgg                                                                <  2:479073/142‑1 (MQ=255)
                                                                         aCAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGACATCTGCGATGATGCAGCTGGGTGTc                                                           >  1:249432/1‑143 (MQ=255)
                                                                         aCAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTc                                                           >  2:341327/1‑143 (MQ=255)
                                                                          cAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCg                                                          <  2:576113/143‑1 (MQ=255)
                                                                          cAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTCCGATGATGGAGCTGGGTGTCg                                                          >  1:327348/1‑143 (MQ=255)
                                                                           aaGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTGAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGCGTCga                                                         >  2:459626/1‑143 (MQ=255)
                                                                           aaGCGTCTCTCGCCAAAAGAAAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGAAGAAGCTGGGGGTCga                                                         >  2:399479/1‑143 (MQ=255)
                                                                             gCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCgcga                                                       >  1:373820/1‑143 (MQ=255)
                                                                              cGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGAGGAAGACGGGTGTCGAGaa                                                      >  2:245906/1‑143 (MQ=255)
                                                                                           aaGAGAGTGAAGTTCTGCGCCGGTTGGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCctg                                         <  1:648285/143‑1 (MQ=255)
                                                                                             gagggggAAGTGCGGCGCCGGTTTGCGAGAGGCTTCTTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCGTCAGTAGCCAGAAGAAACCTGCGGTGATGAAGCTGGGTGTCGAGAACGATATCGCCctgct                                       <  2:173460/137‑1 (MQ=255)
                                                                                                agTGAGGTTCGGCGCCTGTCTGCGGAAGGCTTGATGGTGGGCGAGATCGCTAAAAAGCGGAACCGTAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGaa                                    <  2:98477/143‑1 (MQ=255)
                                                                                                 gTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAAt                                   <  1:19543/143‑1 (MQ=255)
                                                                                                  tggacttCTGCGCCTGTGTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAAtt                                  <  2:509618/138‑1 (MQ=255)
                                                                                                      gTTCTGCTCCTGTGTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGTGTGTCTAGAACGATATCGCCCTGCTGAATTAtct                              <  2:638044/143‑1 (MQ=255)
                                                                                                       ttCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGCACGATATCGCCCTGCTGTATTAtctc                             >  1:139216/1‑143 (MQ=255)
                                                                                                          tGCGCCTGTCTGCGGAAGGCTGCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATctctct                          <  2:464807/143‑1 (MQ=255)
                                                                                                          ggcgtCTGTTTGCGGGAGGCTTCCTGGTGGCCGAGATCGCTAAACAGCTGATCCGCAGTATTAAAGCCATCGGTAGCCAGGAGAAATCTTCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATctctct                          <  2:646526/142‑1 (MQ=255)
                                                                                                           gcgcCTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCtt                         >  2:548048/1‑143 (MQ=255)
                                                                                                              ccTGTTTGCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAACACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAAGTCTCTCTCTTccg                      >  2:359179/1‑141 (MQ=255)
                                                                                                               cTGTTTCCGGAAGGCCTCCTGGTGGCCGAGAGCGCAACAAAGCGGATCCGCAGTATTAACACCATCAGTAGCCAGAATAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGt                     <  1:295875/143‑1 (MQ=255)
                                                                                                                     gCGGACGGCGTCCTGGTTGCCTGGATCGCTAAAAAGCTGAACCGCAGTATTAAAGCCGTCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCCCTCTTCAGTGACCtt               <  2:680308/143‑1 (MQ=255)
                                                                                                                     gCGGAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCtt               <  1:523005/143‑1 (MQ=255)
                                                                                                                        ggAAGGCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCACAGTATTAAAACCATCAGTAGCAAGAAGAAATCTGCGATGATGAAGCTGGGTGTAGAGAAAGATATAGCCATGCGGAATTATCTGTCTTCAGTGCACTTaa             >  2:771742/1‑143 (MQ=255)
                                                                                                                        ggAAGGCGTTCTGGGGGCCGAGATCGCTAGAAATCTGAACCGCAGTATTAGAGCCATCAGTAGCCAGAAGAAATGTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTaa             <  1:169507/143‑1 (MQ=255)
                                                                                                                             gCTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGAAGTCTTAGAACTATCAGTAGCCAGAAGAAATCTGCGATGATGAAGATGGGTGACGAGAACGATATCGACCTGCTGACTTATCTCCCCTCCGTGACCTTAAgcgcg        >  2:56461/1‑139 (MQ=255)
                                                                                                                              cTTCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCgg       <  1:443437/143‑1 (MQ=255)
                                                                                                                                tCCTGGTGGCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCa     <  1:341327/143‑1 (MQ=255)
                                                                                                                                  cTGGTGGCCGAGATCGATAAAAAGCAGAACCGCAGTATCAAAACCATCAGTAGCCAGCAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGa   <  1:305526/143‑1 (MQ=255)
                                                                                                                                   tggtggCCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTCAGTCCGGCAGAt  >  1:526251/1‑143 (MQ=255)
                                                                                                                                        |                                                                                                                                         
CAGAAAGGGAAGAAATTTACCCCGGAAAGCGTTTCTCGCCTGTTGGAAAAAATCAGTGCTGGTGGTTACGGTGACAAGCGTCTCTCGCCAAAAGAGAGTGAAGTTCTGCGCCTGTTTGCGGAAGGCTTCCTGGTGACCGAGATCGCTAAAAAGCTGAACCGCAGTATTAAAACCATCAGTAGCCAGAAGAAATCTGCGATGATGAAGCTGGGTGTCGAGAACGATATCGCCCTGCTGAATTATCTCTCTTCAGTGACCTTAAGTCCGGCAGAT  >  NC_000913/2316544‑2316816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: