| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,527,589 | C→T | Q375* (CAG→TAG) | yrfF → | putative RcsCDB‑response attenuator, inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,527,589 | 0 | C | T | 94.6% | 104.5 / NA | 37 | Q375* (CAG→TAG) | yrfF | putative RcsCDB‑response attenuator, inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base T (20/15); total (22/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCT‑‑GGAGCGCGAAAACCAATTCACCTTTTTTA > NC_000913/3527450‑3527729 | aGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGa < 1:482773/143‑1 (MQ=255) aaaaaCTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTGTATGCTATTATTCTGGATCCCGCTGGATATGCCGCTGGAATTCACTCTCTCATGGATCAAAGGCGCGCAGACAAt > 2:716977/1‑143 (MQ=255) gcatcttgcggTTACAGCACTGGCTGCGCCGTACGATTCTCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATGCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCAtt < 2:403169/133‑1 (MQ=255) aCTTTCCGTTACAGCACTGGCTGCGCAGTACGAGTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGaa < 1:734171/143‑1 (MQ=255) gTTACAGCACTGGCTGCGCATTACGATTATCGCAAAGGGCTCGCTGCTGGTGCAGATTATGCTGTTATACTGGATCCCGATGGATATACCGCAAAAATGAACTCTCTCATGGATGAAAGGCGCGTAGACAATTCAAGccacca > 2:400718/1‑143 (MQ=255) gTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGccacca < 1:164966/143‑1 (MQ=255) gTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGccacca < 1:324773/143‑1 (MQ=255) tACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTCTGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTAATGGATGAAAGGCGCGTAGAGCATTGCAGCCACCAGc > 1:494968/1‑143 (MQ=255) tcTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTaaaa < 1:366110/142‑1 (MQ=255) gcAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCt > 1:692905/1‑143 (MQ=255) gTACGGTTATCGCTGCGGGATCGCTGCTGGTGCTGTTTGTGCTGTTATGCTGGATCCCGCTGGATATGCCGCTGAAACTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAACACAACTGGCTGAc < 1:102277/143‑1 (MQ=255) gATTATCGCTGCGGGCTCGCTGCAGGTGCAAATTAAGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGGAAGGCGCGTAGACCATTGAAGCCAACAGCGTAAAACAACTGGCTGACGcag > 2:238300/1‑141 (MQ=255) tCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGa < 1:309482/143‑1 (MQ=255) gctgctGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGGAGGCGATa > 1:148006/1‑143 (MQ=255) cttgTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATAGGCCGCTGAAATTCACTCTCTCATGGAGGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACAtt < 1:207455/140‑1 (MQ=255) gCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGGTACATTGCGTa > 2:451145/1‑143 (MQ=255) gtctATGCGGTGATTCTGGATCCCGCTGGATATGCCGCGGAAATACACTCTCTCATGGATGAAAGGCGCGTAGACCATTGACGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGGAGGCGATACATTGCGTATTa < 2:418792/140‑1 (MQ=255) gTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAGCAACCGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTa > 1:761404/1‑143 (MQ=255) tGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGt > 2:81308/1‑143 (MQ=255) gCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATCCATTGCGTACTAGCGGtc > 1:743044/1‑142 (MQ=255) gCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGCATTAGCGGTa > 1:781132/1‑143 (MQ=255) tGTTACTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACg < 1:213969/143‑1 (MQ=255) gTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACgg > 2:16927/1‑143 (MQ=255) tgcaTCCCGCTGGAGAGGCCGCGGAAATTCACTCGCTCATGGATGAAAGGCGCGTAGGCCATTGACGCCCCCAGCGTAAAACATCTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAAtgtg < 1:505279/140‑1 (MQ=255) tGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAGtgtg > 1:729320/1‑143 (MQ=255) gATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGAGTa > 2:122235/1‑143 (MQ=255) tCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCAACCGCGTACAACAACTTGCTGACGCTGGCGTGCGTGTAGGCGATACATTGCGTATTAGCGGTACTGGTATGTGTAat > 2:653419/1‑143 (MQ=255) cTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTCGCGGTACGGGAATGGGTAATATTCGAACTTCCGGAAc > 2:362908/1‑143 (MQ=255) cTGAAATTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAAc < 1:487973/143‑1 (MQ=255) aaaTTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCT‑‑g > 1:771077/1‑143 (MQ=255) aTTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGaa < 2:160601/128‑1 (MQ=255) aTTCACTCTCTCATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGaa > 1:160601/1‑128 (MQ=255) tcATGGCTGAAAGGCGCGTAGACCATTGAAGCCACTAGCGTAAAACAACTGGCTGACGATGGCGTGCGGGTAGGCGATACATTTCGTATTAGGGGTAAGGGAATGTGTACCATTCGTACTTCTGTAACCTAGGGAGCGAGaaa > 2:602446/1‑143 (MQ=255) tcATGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCT‑‑GAAGCGCGaatga > 1:211305/1‑140 (MQ=255) aTGGATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCT‑‑GGAGCGCGAAAACCa > 1:251000/1‑143 (MQ=255) ggATGAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGCATGTGTAATATTCGAACTTCAGCAACCT‑‑GGACCGCGAAACCAAAt > 2:675835/1‑143 (MQ=255) gAAAGGCGCGTAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCT‑‑GGAGCGCGAAAACCAATTCAc < 1:735384/143‑1 (MQ=255) cgcAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACACTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGACCCT‑‑GGAGCGCGAAAACCAATTCACCTTTTTTa < 1:249092/143‑1 (MQ=255) | AGTAAAAAACTTTCCGTTACAGCACTGGCTGCGCAGTACGATTATCGCTGCGGGCTCGCTGCTGGTGCTGTTTATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCGTATTAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCT‑‑GGAGCGCGAAAACCAATTCACCTTTTTTA > NC_000913/3527450‑3527729 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |