Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 3,527,656 Δ5 bp coding (1190‑1194/2136 nt) yrfF → putative RcsCDB‑response attenuator, inner membrane protein

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3527656 3527660 5 27 [0] [0] 27 yrfF putative RcsCDB‑response attenuator, inner membrane protein

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 35276550 (0.000)27 (0.600) 23/284 1.1 100% coding (1189/2136 nt) yrfF putative RcsCDB‑response attenuator, inner membrane protein
?NC_000913 3527661 = 0 (0.000)coding (1195/2136 nt) yrfF putative RcsCDB‑response attenuator, inner membrane protein

ATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3527523‑3527655
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAAC  >  NC_000913/3527661‑3527793
                                                                                                                                                                                                                                                                          
ATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGGAGGCGATACATTGCAGCGGTACGG                                                                                                                             >  1:540663/1‑143
    TGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGTAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAAATGGCTGACGCTAGCGTGCGGATAGGCGATACATTGCAGCAGTACGGGAAT                                                                                                                         >  1:621881/1‑143
         ttttcGATCCCGATGGATCTCATCCTGAAATTCCCTCTCTCATGCATGAAAGGCGCGCAGACCATTGAACCCACCAGCGTAAAACAACTGCCTGACGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTA                                                                                                                    <  1:39885/138‑1
          TCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTAA                                                                                                                   <  2:366379/143‑1
            cGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTAATA                                                                                                                 <  1:247293/142‑1
                     CTGGATATGCCGCTGAAATTCACTCTCTCATGGGTGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGGTACATTGCAGCGGTACGGGAATGTGGAATATTCGAAgtg                                                                                                        >  2:31976/1‑140
                                      ATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGACACATCGCAGCGGTGCGGGAAGGTGTAATATTCCGACTTCCGGGACCTGGGGGGCG                                                                                       >  2:396494/1‑143
                                                   TGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCAC                                                                          <  1:611258/143‑1
                                                    GGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACC                                                                         <  1:341578/143‑1
                                                        GAAAGGCGCGTAGATCTTTGAAGTTATCAGAGTTAAACAACTGGCTGACGTGGGCGTGTGGGTAGGCGATACATTGCAGCGGTAAGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTT                                                                     <  2:405608/143‑1
                                                        GAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCGATTCACCTTTT                                                                     >  1:15808/1‑143
                                                        GAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTT                                                                     >  1:461636/1‑143
                                                             GCGCGCAGACCATTGAAGGCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACC                                                                <  2:508764/143‑1
                                                              CGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCG                                                               <  1:61305/143‑1
                                                                 GCAGACCAGTGAAGCCACCAGCGTAAAACACCTGGCGGACGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTT                                                            <  2:461636/143‑1
                                                                   AGACCAGTGAAGCCCCCAGCGTAAGACAACGGGCGGACGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGA                                                          <  2:668557/143‑1
                                                                              GCCCCCAGCGTACAACAACTGGCTGCCGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGA                                               <  2:597503/143‑1
                                                                                tgccgGCGTAAACCAACTGGCTGACGCGGGCGTGCGGGTAGGCGATACGTTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAACACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATC                                             <  2:33250/138‑1
                                                                                cagCAGCGTAACATAACGGGCTGCCGCTGGCGTGCGGGTAGGCGATACCTTGCAGTGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAACACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATC                                             <  2:275380/140‑1
                                                                                      CGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTTCTCtccggtcctccgg                                       >  1:414802/1‑130
                                                                                       GTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATCCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGA                                      >  2:267919/1‑143
                                                                                        TAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGCAGCGGTACGGGAATGTGTAATGTTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCCGATAATCAGGAA                                     >  2:232177/1‑143
                                                                                                         GCTGGCGTGCGGGTAGGCGATACACTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGC                    <  1:268506/143‑1
                                                                                                                   GGGTAGGCGCTACATTGCAGCGGTACGGGTATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGGTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGA          <  2:606611/143‑1
                                                                                                                      TAGGCGATACATTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGACCATCTGGAACGACGCCCGCTCATTGCCATTCCCAGAATC       >  2:373381/1‑143
                                                                                                                           GATACATTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCTTTGCGTTTGCCAGAATCTacac  >  1:322812/1‑139
                                                                                                                           GATACATTGCAGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAAC  >  1:414337/1‑143
                                                                                                                                                                                                                                                                          
ATGCTGTTATTCTGGATCCCGCTGGATATGCCGCTGAAATTCACTCTCTCATGGATGAAAGGCGCGCAGACCATTGAAGCCACCAGCGTAAAACAACTGGCTGACGCTGGCGTGCGGGTAGGCGATACATTGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3527523‑3527655
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCGGTACGGGAATGTGTAATATTCGAACTTCCGGAACCTGGAGCGCGAAAACCAATTCACCTTTTTTACCGTTTGACTGCTCGCAGATCATCTGGAACGACGCCCGCTCATTGCCATTACCAGAATCTGAAC  >  NC_000913/3527661‑3527793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.