Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	3,992,523	(CGGGC)2→1	coding (1372‑1376/2547 nt)	cyaA →	adenylate cyclase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3992521 =	1 (0.020)	38 (0.890)	37/270	0.1	97.5%	coding (1370/2547 nt)	cyaA	adenylate cyclase
	?	NC_000913	= 3992515	1 (0.020)					coding (1364/2547 nt)	cyaA	adenylate cyclase

GGTGCGCGAGGTCAGCAGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3992666‑3992521 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcccggcGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGATATCCTG  <  NC_000913/3992515‑3992388                                                                                                                                                                                                                                                                                    GGTGCGCGAGGTCAGCACGCCGTTACACCATGCCCGCGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCC                                                                                                                                     <  2:579341/143‑1  GTGCGCGAGGTCAGCAGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCCACATGAACGGTTCGCCCG                                                                                                                                    >  1:756471/1‑143    GCGCGTGTTCAGCATGCGGTTAAACCTCGCCCACTCTACCAGTTTATTCAGGTAACTGTCATATTCCATCTGCTGTTGGCTGATTATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGC                                                                                                                                  <  2:388578/143‑1       CGAGGTCAGCAGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGC                                                                                                                               >  1:510090/1‑143        GAGGTCAGCAGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCA                                                                                                                              >  1:77639/1‑143                CAGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACGTAAATg                                                                                                                      >  1:304696/1‑142                 AGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTACAGCGGATGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATAAAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAA                                                                                                                     >  1:363094/1‑143                   GCCGTTAAACTATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAG                                                                                                                   <  1:393541/143‑1                     CGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGCTATATTCCAGCGGCTGATGGCTGATGATCGACTCACTATTTGGCGCGCGGTTATACACATACCAACCTGAACGGTTAGCCCGGCGGCACACAAAAAAAGaa                                                                                                                 >  2:51709/1‑141                        TAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAG                                                                                                              >  1:131505/1‑143                              tgGCCCGCGCCACCAGTTGATTCAGTTAACGTTTATATTCCATCGGCTGATGGCTGATGATCGACTCAAGATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGG                                                                                                        <  2:44065/141‑1                                  CCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCCACCGGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTGCC                                                                                                    >  2:555001/1‑143                                    ACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGA                                                                                                  <  1:522320/143‑1                                          gCAGTTGATTCGGGTACCGGTGATATTCCAGCGGCTGAGGGCTGATGATGGACTCAATAGTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATC                                                                                            <  2:487156/142‑1                                           CAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCG                                                                                           >  1:3607/1‑143                                            AGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTGAGCCCGGCGGCACATAAATAAAGGTCAGATGCGGTTCCGAGAGATCGG                                                                                          >  2:173612/1‑143                                              TTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGT                                                                                        <  1:713159/143‑1                                              TTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGACGCGCGGGTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCCGATTCCGATCCGAGAGGTCGGGg                                                                                        >  2:729998/1‑142                                                    tAGGTAACGGTTATGTTCCAGCGGCTGATGGCTGGTGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAACGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATC                                                                                  <  2:90848/142‑1                                                    CAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCAGAACGGTTAGCCCGGCGGCCCATAAATAAAGGGCAGATTCGGTTCCGAGAGATCGGGCGAAATC                                                                                  >  2:633471/1‑143                                                           CGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGGGAAATCTGCGGGT                                                                           >  2:510693/1‑143                                                                          GGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTT                                                            <  2:77639/143‑1                                                                              GATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCAGCGGCACATAAATAAAAGACAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACC                                                        >  1:306211/1‑143                                                                                       tgtTCGACTCAGTATTTGGCGCTCGGTAATCAAAGTACTAACCTGTGCGGTTAGCGCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGC                                               <  2:343963/140‑1                                                                                              CTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGG                                                                                         >  2:646402/1‑94                                                                                              CTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGG                                                                                         <  1:646402/94‑1                                                                                               TCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGCTCGGGAGAAATCTGCGGGTTTAACAGCGTCACTTTACCCGGTAATGCTTCAAACG                                       >  2:418713/1‑143                                                                                                 AATCTTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCG                                     <  1:467814/143‑1                                                                                                    ATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGATTGGCCGCATCACTGTACCTGGTAACGCTTCAAACCCGGCA                                  >  1:739861/1‑143                                                                                                         GCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCCGCGTACAG                             >  1:483984/1‑143                                                                                                         GCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGACAGATGCGGTACCGAGAGAGCGGGTGAAATGTGCGGGTTTACCAGCGTCGCGTTAGCTGGTAATGCTTCACACGCGGCgtgcgg                             >  1:93569/1‑137                                                                                                          CGCGCGGTGATACAGAGACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGC                            <  2:155577/143‑1                                                                                                            CGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGGCGCTTTACCTGGTAATGCTTCAAACGCGGCATACCGCTT                          >  2:109839/1‑143                                                                                                              CGGTTAGACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTAC                        <  1:460821/143‑1                                                                                                                 TTATACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCAGACAGCTCACGCG                     >  2:673538/1‑143                                                                                                                    TACAGATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGAGTGGGAGCCGAGATGTCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCA                  <  1:178213/143‑1                                                                                                                         ATACCAACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACG             >  1:398917/1‑143                                                                                                                               ACCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGATc       >  1:707732/1‑142                                                                                                                                CCTGAACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGGCAGCACGCCGATAa      >  2:542800/1‑142                                                                                                                                   gagCGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGATATCCT   <  2:363094/140‑1                                                                                                                                    AACGGTTAGCCCGGCGGCACATAAATAAAGGTCAGATGCGGTTCCGAGAGATCGGGTGAAATCTGCGGGGTTACCAGCGTCACTTTACCTGGGAATGCTTCAGACGCGGCGTACCGCTTACGCGTCAGCACGGCGATCTCCTG  >  2:78777/1‑143                                                                                                                                                                                                                                                                                    GGTGCGCGAGGTCAGCAGGCCGTTAAACCATGCCCACGCCACCAGTTTATTCAGGTAACGGTTATATTCCAGCGGCTGATGGCTGATGATCGACTCAATATTTGGCGCGCGGTTATACAGATACCAACCTGAACGGTTAGCCCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/3992666‑3992521 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcccggcGGCACATAAATAAAGGTCAGATTCGGTTCCGAGAGATCGGGTGAAATCTGCGGGTTTACCAGCGTCACTTTACCTGGTAATGCTTCAAACGCGGCATACAGCTTACGCGTCAGCACGCCGATATCCTG  <  NC_000913/3992515‑3992388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.