Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	130.9 / NA	44	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (23/21);  total (23/21)

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132650‑2132926                                                                                                                                          |                                                                                                                                            tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                        >  1:81775/1‑143 (MQ=255)    ggatgcgggTTATCAGCGTATATTTTTTGGTGGCGGGGTCGAATGTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTCATATGCAGGCTCTGCGTc                                                                                                                                     <  2:599918/137‑1 (MQ=255)     gTAGAGCGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGTGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                    <  1:762916/143‑1 (MQ=255)      tAGAGGGTAAGCAGCTGATATTTGTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                   <  2:635275/143‑1 (MQ=255)         agGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTGCACCGTGATTGATATGCAGGCTCTGCGTCGccca                                                                                                                                >  1:542620/1‑141 (MQ=255)         agGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCAGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctc                                                                                                                                >  1:338475/1‑143 (MQ=255)            gTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCAGTGCACCGTGATTGATATGCAGGCTCTGCGTCGCCccttc                                                                                                                             >  1:358368/1‑143 (MQ=255)              aaaCAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTCTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                           >  2:633598/1‑143 (MQ=255)                   gCTGTTATTTTTGGCTGGCGCTGTCGTGGTTGTCGTTGTGCTGGCGGTGAGAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCGCTTCTACTTTc                                                                                                                      <  2:730419/143‑1 (MQ=255)                     tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                    >  2:624099/1‑143 (MQ=255)                     tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGCCGCCTCTTCCACTCTCCa                                                                                                                    >  2:3368/1‑143 (MQ=255)                           tttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTGCACGGTTc                                                                                                              >  1:96669/1‑143 (MQ=255)                              tggctggctgagtggAACTTGTTTTTGTGCTGGCGGTAAAAGTGGAAGTATCTGGAGAATTTCTTCGGTGACGAGTTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGcc                                                                                                           <  2:322762/129‑1 (MQ=255)                                      gcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcca                                                                                                   >  1:519210/1‑143 (MQ=255)                                      gcgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcca                                                                                                   <  2:222870/143‑1 (MQ=255)                                       cgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac                                                                                                  <  1:16446/143‑1 (MQ=255)                                              ttttGTCTTTGTGCTGGCGGTAAAAGTGGAAGTCCCCTGCGAATTTTTGCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCCCATACGTTCCACGTGTCGCCGTACTCCACCACCATc                                                                                           <  2:241912/142‑1 (MQ=255)                                              gtgtGTCTTTGTGCGTGCGGTAGAGGTGGAAGTACTCGGAGAAGTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATc                                                                                           <  2:633676/140‑1 (MQ=255)                                                      ttGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                                   <  1:523928/143‑1 (MQ=255)                                                      ctgtgCTTGCGGCAAAAGTGGAAGTACCCGGACAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGCTTGATACGCCGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGaa                                                                                   <  1:218573/142‑1 (MQ=255)                                                           cTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGGTATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAGTCCCCCACCATCCGCAGCAAGCTGt                                                                              >  1:761792/1‑143 (MQ=255)                                                                       gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                  <  2:338475/143‑1 (MQ=255)                                                                             gTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCtt                                                            >  2:168683/1‑143 (MQ=255)                                                                              ttccttcagtagttttgCGTTGCCTAGGTGATCTGCCTTTCACCGTGATTGTTTTGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTGCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCttt                                                           <  2:703729/131‑1 (MQ=255)                                                                                 ccGGAGCATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAg                                                        <  1:450809/143‑1 (MQ=255)                                                                                 ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCCTTCAg                                                        >  2:166259/1‑143 (MQ=255)                                                                                           ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCCGGGAGATGTCGGACTCCTGTGCCGCTTCCAGTTCGCTATCg                                              >  1:582796/1‑143 (MQ=255)                                                                                           ttgtgggTGACGAGGTGATCTGGATTTCTCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCg                                              <  2:641831/138‑1 (MQ=255)                                                                                             ttCGGTGACGAGGTGATCTGCATTTCACCGTGATCGACATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa                                            <  1:452769/143‑1 (MQ=255)                                                                                                 ttggcggggtcgtCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaa                                        <  2:241101/131‑1 (MQ=255)                                                                                                   gactatgTGATGTGCATTTCTCCGTGATTGAGATGCATGCTTTGCGTCGCCTCTTCTACTTTCCGCGGTTTGCCGTACTTGCCCACCTTCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                      <  2:381405/137‑1 (MQ=255)                                                                                                    aCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTCTCAGTGCGGTATCGAACAAacac                                     >  2:647849/1‑143 (MQ=255)                                                                                                              cTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCTAACAAACACTCTTTGAAAc                           >  1:488316/1‑143 (MQ=255)                                                                                                               tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCTACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCACTTCGGGCTCGAACAAACACTCTTTATAACg                          >  2:152706/1‑143 (MQ=255)                                                                                                                gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGc                         >  1:309873/1‑143 (MQ=255)                                                                                                                gCATTTCACCGGGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTGGCCGTGCGCCCCCACTATCCGCAGGAAGATGTCGTAATCCTGTGGAGCATTAAGTTCGGTAGCGAAGAAagacgggttggaacga                         >  1:171727/1‑131 (MQ=255)                                                                                                                    ttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGGCGTACTCCACCAACATCTGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGCACAGACACTGTTTGAGAGGCCATg                     >  1:109345/1‑143 (MQ=255)                                                                                                                        ccGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGACACGCCATGCCCa                 >  1:726541/1‑143 (MQ=255)                                                                                                                         gttgattgctATGCTGTCTCTGCTTCGCTTCTTCTACTTTCCTCGGTTCGCCGTACGCCACCTCCATCGGCATGACGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACATTCTTTGAAACGCCATGCCCAg                <  2:394093/141‑1 (MQ=255)                                                                                                                          tcgatcggtctGCAGGCTCCGCGCCGCCTTTTCTACTTTCCACGGGTCGCCGTACTCCACCACCATCCTCAGGCAGATGTCGTAATCCTGTGCCGCTTCCAGTGCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAgg               <  2:566124/133‑1 (MQ=255)                                                                                                                               tgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGATCGCCGTACTCCACCACCATCCGCAGGAAAATGTCGTAATACTGTGCCGCTTTCAGTTCGGTATCGACCAAACACTATTTGAAACGCCATGCCCAGGTaagg          >  2:24610/1‑141 (MQ=255)                                                                                                                                   atGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGTTATCGAACAAACACTCTTTGAAACGCTATGCCCAGGTAAAGACCt      >  1:612083/1‑143 (MQ=255)                                                                                                                                    tGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTg     >  1:625109/1‑143 (MQ=255)                                                                                                                                       aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGtt  <  1:624099/143‑1 (MQ=255)                                                                                                                                          |                                                                                                                                            TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132650‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑