Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,316,391	T→C	Y73H (TAC→CAC)	rcsB →	response regulator in two‑component regulatory system with RcsC and YojN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,316,391	0	T	C	100.0%	136.8 / NA	50	Y73H (TAC→CAC)	rcsB	response regulator in two‑component regulatory system with RcsC and YojN
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/18);  total (32/18)

AGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGATCTGCCGAAA  >  NC_000913/2316254‑2316528                                                                                                                                          |                                                                                                                                          aGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTATCCATACCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATc                                                                                                                                      >  1:219412/1‑143 (MQ=255)      gTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAgcg                                                                                                                                 >  1:414352/1‑143 (MQ=255)       tGAATGTTGTCGGCGAATTTGAAGACGCGACAGCACTGATCAACCTCCTGCCGAAACTGGATGCGCATGTGTGGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAgcgc                                                                                                                                <  2:751688/143‑1 (MQ=255)         aaTGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGATACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAACGccc                                                                                                                              >  2:418359/1‑142 (MQ=255)             ttgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGGAACTGGATGCGCATGTGTTGAAGACCGACCGCTCCATGCATGCCGATAAGTACGGCGATGGCATTACCTCAAGCAGGCGCAGCAAGCGCCACTTc                                                                                                                          >  2:51451/1‑143 (MQ=255)             ttgtCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTc                                                                                                                          <  1:419603/143‑1 (MQ=255)                  ggCGAATTTGAAGACTTTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAg                                                                                                                     <  2:716212/143‑1 (MQ=255)                   gCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGCTGGCATTACCTTAATCAAGCACATCACGCGCCATTTCCCAAGc                                                                                                                    >  2:542463/1‑143 (MQ=255)                   gCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGc                                                                                                                    >  1:253619/1‑143 (MQ=255)                    cGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCACGcc                                                                                                                   >  1:320461/1‑143 (MQ=255)                     gAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCt                                                                                                                  >  2:579488/1‑143 (MQ=255)                       aTTTGAAGACTCGACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGt                                                                                                                <  2:180343/143‑1 (MQ=255)                               acgctACAGCACTGATCAAGAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCAtt                                                                                                        <  1:222464/140‑1 (MQ=255)                                 tctACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGAATGACCCTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGt                                                                                                      >  1:570940/1‑143 (MQ=255)                                 tctACAGCACTGATCAACAACCTGCCAAAACTGGATGCGCATGTGTTGATGACCGATCTCTCCATGCCAGGCGATAAGTACGGCGATGACACTACCTTAATCACGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCAtggc                                                                                                      >  2:72385/1‑140 (MQ=255)                                  ctACAGCACGGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGtt                                                                                                     <  1:295505/143‑1 (MQ=255)                                   tACAGCGCTGATCAAGAACGTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGAGCCATTTCCCAAGCCTGTCGATCATTGTTc                                                                                                    <  2:280169/143‑1 (MQ=255)                                    aCAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGGTAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCt                                                                                                   >  1:416894/1‑143 (MQ=255)                                    aCAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATACGTACGGCGCTGGCATTACCTTAATCAAGCACATCAGGCGCCATTTCCCAAGCCTGTCGAGCCTTGTTCt                                                                                                   >  2:102430/1‑143 (MQ=255)                                         aCTGATCAACACCCTGCCGAGACTGGATGCGCATCTGTTGATTACCGCTCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTa                                                                                              <  2:518977/143‑1 (MQ=255)                                         aCTGATCAAAAGCCGGCCGAAACGGGATGCGCATGGGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTa                                                                                              <  2:247163/143‑1 (MQ=255)                                           tgacccacagcCCGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTCCCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATg                                                                                            <  2:33870/137‑1 (MQ=255)                                             aTCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGCCGATCATTGTTCTGACtctcaa                                                                                          >  2:81446/1‑138 (MQ=255)                                                          cGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCg                                                                             >  2:18962/1‑143 (MQ=255)                                                           gAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCCCCTCAGGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAATCCGgaga                                                                            >  1:91781/1‑143 (MQ=255)                                                           gAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCCAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGAAAAAAAACAACAACACGGCGa                                                                            >  1:448242/1‑143 (MQ=255)                                                             aaCTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGCTGGCCTTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGAta                                                                          >  1:494833/1‑142 (MQ=255)                                                              aCTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTACTCACGCACCTCCAGCGCCATTTCCCCAGCCTGTCGATCATTGTTCTGCCTACGAACAACAACCCGGCGAtcc                                                                         >  2:251883/1‑141 (MQ=255)                                                                 ggATGCGGATGTGTTGATCACCGATCGCTCCATGCCTGGCGATAGGTACGGCGATGGCATTACCTTAACCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTa                                                                      <  1:144856/143‑1 (MQ=255)                                                                   aTGCGCATGTGTTGATTACCGATCTCTCCATGTCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCAAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCcgacgacactcatt                                                                    >  2:71532/1‑135 (MQ=255)                                                                    ggcgcGTGTGTTGATGACCGCTCGCTCCCTGCCTGGCGATAAGTACGGCGATGGCATTAGCTTCATCAAGCACATCGAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTg                                                                   <  2:320705/142‑1 (MQ=255)                                                                       gcATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCCGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTAAGTGCgg                                                                >  1:630062/1‑143 (MQ=255)                                                                           gtgtTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTAtt                                                            <  2:317741/143‑1 (MQ=255)                                                                            tgtTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAGGCCGGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTg                                                           >  1:292068/1‑143 (MQ=255)                                                                               tGATTACCGATCTCTCCATGCCTGGCGATAAGTAAGGCGATGGCATTACGTGAATCAAGCAGATCAAGCGCCAATTGCCAAGCCTGTCGATCATTGTGCTGACTATGAACAACGAACCGGGGATTCTTAGTGCGGTATTggct                                                        >  1:301132/1‑141 (MQ=255)                                                                                  ttACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGACATTACCTTAATACTGCATATCTTGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTg                                                     <  1:780026/143‑1 (MQ=255)                                                                                     ccGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACAGCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACGACAACCCGGCGATTCTTAGTGAGGTATGGGGTCTGGat                                                  >  2:284355/1‑143 (MQ=255)                                                                                      cGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTCTTGGATCTGGgta                                                 >  1:344288/1‑143 (MQ=255)                                                                                        aTCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACCACACACCGGCGATTCTTAGTGCGGTATTGGATCAGGGTATc                                               >  2:714774/1‑143 (MQ=255)                                                                                           tctcCATGCCTGGCGATAAGTACGTCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGaa                                            <  1:261736/143‑1 (MQ=255)                                                                                           tctcCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCGTTGTTGTAACTATGAACAACAACCCGGCGCTTCTTAGTGCGGTATTGGCTCTGGAGACCGaa                                            >  2:700443/1‑143 (MQ=255)                                                                                               cATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGa                                        >  2:530161/1‑143 (MQ=255)                                                                                                 tGCCTGGCGATAAGTTCGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACACCCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATc                                      <  1:579488/143‑1 (MQ=255)                                                                                                  gCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGAGCATTGTTCTGACTATGAACAACAACCCGGCGCTTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCg                                     >  1:582521/1‑143 (MQ=255)                                                                                                               tACGGCGATGGCATTCCCTTAATCAAGCACATCAAGCGCCATTTCCCAAACCTGTCGCTGATTGTTCAGAATATGAACAACAACCAGGCGATTCTTCGTGCGGTATTGGAACTGGATATCAAAGGGATCGGGCGGaagaacgg                        >  2:663452/1‑137 (MQ=255)                                                                                                                 cGGCGATGGCATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACACggcg                      >  1:764343/1‑141 (MQ=255)                                                                                                                          cATTACCTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCaccgaccg             <  2:486433/143‑1 (MQ=255)                                                                                                                          cATTACCTTAATCAAGCACATAAAGAGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGAGATTCTTAGTGCGGTATTGGATCCAGATATCGAAGGGATCGTGCTGAAACAAGGTGCaccgaccg             <  2:626847/143‑1 (MQ=255)                                                                                                                                cTTAATCAAGCACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGACGATTCTTAGTGCGGTATTGTAACTGAATATCGAAGGGAACGTGCTGAAAAAAGGTGAACCGACCGATCTGc       >  2:70319/1‑143 (MQ=255)                                                                                                                                     tCAAGCACATCAAGCGCCATTTCCCAAGCATGTCGATCATTGTTCTGACTATGAACAACAACACGGCGAATCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAACCAAGGTGCACCGCCCGATCTGCCGaaa  >  2:459742/1‑143 (MQ=255)                                                                                                                                          |                                                                                                                                          AGTGGGTGAATGTTGTCGGCGAATTTGAAGACTCTACAGCACTGATCAACAACCTGCCGAAACTGGATGCGCATGTGTTGATTACCGATCTCTCCATGCCTGGCGATAAGTACGGCGATGGCATTACCTTAATCAAGTACATCAAGCGCCATTTCCCAAGCCTGTCGATCATTGTTCTGACTATGAACAACAACCCGGCGATTCTTAGTGCGGTATTGGATCTGGATATCGAAGGGATCGTGCTGAAACAAGGTGCACCGACCGATCTGCCGAAA  >  NC_000913/2316254‑2316528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑