Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 759,776 C→A 59.6% D357E (GAC→GAA)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913759,7760CA59.6% 29.7 / 93.5 64D357E (GAC→GAA) sucA2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base C (9/17);  new base A (17/21);  total (26/38)
Fisher's exact test for biased strand distribution p-value = 4.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.07e-01

AACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTCCGTAACACCTTTAAACGTGATGTCTTCATCGACCTG  >  NC_000913/759498‑760061
                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                             
aacgtgctggtgaacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTg                                                                                                                                                                                                                                                                                      >  2:37555/1‑288 (MQ=255)
          tgaacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGGGACGCCGCAGTGACCGGGCAggg                                                                                                                                                                                                                                                                           >  2:318578/1‑289 (MQ=255)
           gaacgtgctggGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAggg                                                                                                                                                                                                                                                                           <  1:103787/288‑1 (MQ=255)
             acgacctggGTAAAAAACCCCAAGCATTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTGGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGGGATTGTAAGCCCGGTAGTGATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCg                                                                                                                                                                                                                                                                         <  2:238075/283‑1 (MQ=255)
                     ggTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAgg                                                                                                                                                                                                                                                                >  1:134242/1‑289 (MQ=255)
                             aCCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAgg                                                                                                                                                                                                                                                                <  1:423599/281‑1 (MQ=255)
                             aCCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAgg                                                                                                                                                                                                                                                                >  2:423599/1‑281 (MQ=255)
                               cGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGaa                                                                                                                                                                                                                                                      <  1:81389/289‑1 (MQ=255)
                               cGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGaa                                                                                                                                                                                                                                                      <  1:313853/289‑1 (MQ=255)
                               cGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGa                                                                                                                                                                                                                                                       >  2:302974/1‑288 (MQ=255)
                               cGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGCAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGAAGCAACAAAGTGCTGCCAACCACCCTCTACGGTGAAGCCCCAGTCACCGGGCAGGGCGTGGGTCAGGAAACACTGa                                                                                                                                                                                                                                                       >  1:238085/1‑288 (MQ=255)
                                ggaagacagatgcgaggggatagCCGGTGAACATGAAGAGGCCCGCAGGACGGGTGAGCGGAAATAGCAGGTGGGGGTTGCGTCTAAGATCCAGAGTGAGGTCGGCGTGGAGCACCTGGCGCTGGAGTTGAACCCGGCTCACCTTGAGAGTGTGAGCCGGGTGGTTATCGGGTCGGTTC‑GTGAACGTCTAGACAGACTTGATGAGACGAGCAGCAACAACGTGCTGCCAATCACCGTCCAGGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGaa                                                                                                                                                                                                                                                      <  1:371430/266‑1 (MQ=255)
                                       ttGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGa                                                                                                                                                                                                                                              <  2:392939/289‑1 (MQ=255)
                                               cgggtgcGCCGGTAAACATAAAGAACACCGCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGcgcg                                                                                                                                                                                                                                       <  1:207846/282‑1 (MQ=255)
                                               cgaGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGcgcg                                                                                                                                                                                                                                       <  2:338460/288‑1 (MQ=255)
                                                                  aaaGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGcggta                                                                                                                                                                                                                   <  1:318578/289‑1 (MQ=255)
                                                                  aaaCAACACCCCGGCGCGGGGCACGAGAAGGTCCACATGGGCATCTCGTCGGAGGTCCGGAGTGATGGCGGCCTAGGGCACCTGGGCCTTGCTTTTAACCCGACGCAACTTGAAATTGTAATCCCGGGAGTTATCGGTTCTGTTC‑GTGCGCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCGGCCAAGGACCATCAACGGGGACGGCGCAGTGACCGAGCAGGGCGTGGTTCAGGAAACCCTGAACAGGTCGAAAGCGCGTGGTTAGGAAGTTGGcggta                                                                                                                                                                                                                   <  1:255413/289‑1 (MQ=255)
                                                                   aaGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGcggtac                                                                                                                                                                                                                  <  2:134242/289‑1 (MQ=255)
                                                                         acCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGgtacggtacg                                                                                                                                                                                                            >  1:361175/1‑289 (MQ=255)
                                                                           cTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTa                                                                                                                                                                                                          >  2:204322/1‑289 (MQ=255)
                                                                                cACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGcgcg                                                                                                                                                                                                                                       <  2:396565/255‑1 (MQ=255)
                                                                                cACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGcgcg                                                                                                                                                                                                                                       >  1:396565/1‑255 (MQ=255)
                                                                                    ggTGACGTGAAATACCCCATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATc                                                                                                                                                                                                  <  1:94185/288‑1 (MQ=255)
                                                                                     gTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATca                                                                                                                                                                                                 >  2:334868/1‑288 (MQ=255)
                                                                                             aaaaacaccaTGGGCTTCCGGTCTGACTTCCCGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGGCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAgg                                                                                                                                                                                        <  1:267940/281‑1 (MQ=255)
                                                                                                  ccACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGCCGAAAGCGCGTGGTTATGAAGTTGGCGGAACGGTACGTATCGTTATAAACCACCAggttggt                                                                                                                                                                                   >  1:221222/1‑289 (MQ=255)
                                                                                                    acaTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTgg                                                                                                                                                                                                                        <  2:119300/250‑1 (MQ=255)
                                                                                                    acaTGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTgg                                                                                                                                                                                                                        >  1:119300/1‑250 (MQ=255)
                                                                                                           cTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGTCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTcaccac                                                                                                                                                                           <  1:230525/288‑1 (MQ=255)
                                                                                                                    tGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATcc                                                                                                                                                                  >  2:259864/1‑288 (MQ=255)
                                                                                                                            aGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGc                                                                                                                                                         <  1:37555/289‑1 (MQ=255)
                                                                                                                                 gATGGCGGCCTGGTGCACCTGGCGCTGGCTTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGtt                                                                                                                                                    <  2:223980/289‑1 (MQ=255)
                                                                                                                                 gATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGtt                                                                                                                                                    <  2:427831/289‑1 (MQ=255)
                                                                                                                                  aTGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTCCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGt                                                                                                                                                     >  1:32079/1‑287 (MQ=255)
                                                                                                                                     gcggcCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTa                                                                                                                                                 <  1:203718/288‑1 (MQ=255)
                                                                                                                                       ggcCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGGGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACg                                                                                                                                               <  2:221222/288‑1 (MQ=255)
                                                                                                                                           tgggggaCCTGGGCCTGGCGGTTAACCCTGTTGACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCGAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCgta                                                                                                                                          <  1:452570/283‑1 (MQ=255)
                                                                                                                                           tGGTGCACCTGGCGCTGGCGTTTAACCCTTCTCACCTTGGGATTTTAAGCCCGGTGGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCgta                                                                                                                                          <  1:259864/289‑1 (MQ=255)
                                                                                                                                           tGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCgta                                                                                                                                          <  1:334868/289‑1 (MQ=255)
                                                                                                                                                       cgctggcgTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGata                                                                                                                               >  1:477770/1‑288 (MQ=255)
                                                                                                                                                       cgctggcgTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGAAAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACAGGGCGGGGCGTGGTTCAGGAAAACCTGAACATGGCGAAAGAGCGTGGTTATGAAGTTGGCGGTACGGGACGTATAGGTATCAACAACCAGGTTGGTTTCACCACCCCTCACCCGCTGGTTGCACGTCCTACGCAGtaatctactgct                                                                                                                                >  1:458757/1‑285 (MQ=255)
                                                                                                                                                             cgTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTaa                                                                                                                        >  2:288008/1‑289 (MQ=255)
                                                                                                                                                                 tAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACg                                                                                                                                               >  2:172339/1‑262 (MQ=255)
                                                                                                                                                                 tAACCCGTCTCACCTTGAGATTGGAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACg                                                                                                                                               <  1:172339/262‑1 (MQ=255)
                                                                                                                                                                                      tGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACg                                                                                                                                               <  2:365811/241‑1 (MQ=255)
                                                                                                                                                                                      tGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACg                                                                                                                                               >  1:365811/1‑241 (MQ=255)
                                                                                                                                                                                        tAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGt                                                                                             >  2:67883/1‑289 (MQ=255)
                                                                                                                                                                                        tAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGt                                                                                             >  2:194456/1‑289 (MQ=255)
                                                                                                                                                                                                     tATCGGTTCTGGTCAGTG‑CCGTCTGGACAGAATTGTTGAGCCGAGCAGCAACAAAGGGCTGCCAGTCACCATCCACGGTGAAGCCGACGTGACCGGGCGGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAGGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGAt                                                                                <  1:302974/289‑1 (MQ=255)
                                                                                                                                                                                                         ggTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCgg                                                                            <  1:204322/289‑1 (MQ=255)
                                                                                                                                                                                                                  c‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTg                                                                   <  1:67883/289‑1 (MQ=255)
                                                                                                                                                                                                                      gCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCt                                                                >  2:326308/1‑289 (MQ=255)
                                                                                                                                                                                                                          gTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTtgt                                                            <  2:361175/289‑1 (MQ=255)
                                                                                                                                                                                                                                              ccGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAACCCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGAt                                         <  2:238085/288‑1 (MQ=255)
                                                                                                                                                                                                                                              ccGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGAtt                                        >  2:330763/1‑289 (MQ=255)
                                                                                                                                                                                                                                              ccGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGAtt                                        <  1:194456/289‑1 (MQ=255)
                                                                                                                                                                                                                                                      caacaaAGGGCGGCCAATCACCATCCACGGTGAAGCCGCATTGCCCGGGCAGGTGGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTCCGTAac                                <  2:458757/289‑1 (MQ=255)
                                                                                                                                                                                                                                                              tgctgcCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCgg                                                                            >  1:150034/1‑237 (MQ=255)
                                                                                                                                                                                                                                                              tgctgcCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCgg                                                                            <  2:150034/237‑1 (MQ=255)
                                                                                                                                                                                                                                                                 tgcCAATCACCATCCACGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGCTTGGCGTCTGATTTCCGTAACACCTTTAAACg                     >  1:238350/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                             tCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTCCGTAACACCTTTAAACGTGATGTCTTCAt         >  1:434312/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                 cGGTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTCCGTAACACCTTTAAACGTGATGTCTTCATCGAc     <  1:326308/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  ggTGAAGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTCCGTAACACCTTTAAACGTGATGTCTTCATCGAcc    <  2:238350/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     gACGCCGAGGTGACCGGGCAGGGCGTGGTTCCGGAACCCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTCCGTAACACCTTTAAACGTGATGTCTTCATCGACCTg  <  1:288008/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                             
AACGTGCTGGTGAACGTGCTGGGTAAAAAACCGCAAGACTTGTTCGACGAGTTCGCCGGTAAACATAAAGAACACCTCGGCACGGGTGACGTGAAATACCACATGGGCTTCTCGTCTGACTTCCAGACCGATGGCGGCCTGGTGCACCTGGCGCTGGCGTTTAACCCGTCTCACCTTGAGATTGTAAGCCCGGTAGTTATCGGTTCTGTTC‑GTGCCCGTCTGGACAGACTTGATGAGCCGAGCAGCAACAAAGTGCTGCCAATCACCATCCACGGTGACGCCGCAGTGACCGGGCAGGGCGTGGTTCAGGAAACCCTGAACATGTCGAAAGCGCGTGGTTATGAAGTTGGCGGTACGGTACGTATCGTTATCAACAACCAGGTTGGTTTCACCACCTCTAATCCGCTGGATGCCCGTTCTACGCCGTACTGTACTGATATCGGTAAGATGGTTCAGGCCCCGATTTTCCACGTTAACGCGGACGATCCGGAAGCCGTTGCCTTTGTGACCCGTCTGGCGCTCGATTTCCGTAACACCTTTAAACGTGATGTCTTCATCGACCTG  >  NC_000913/759498‑760061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑