New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3224520 =	48 (1.250)	3 (0.080)	3/276	5.5	6.1%	coding (1890/3093 nt)	ebgA	evolved beta‑D‑galactosidase, alpha subunit
	?	NC_000913	3224534 =	45 (1.210)					coding (1904/3093 nt)	ebgA	evolved beta‑D‑galactosidase, alpha subunit
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGG  >  NC_000913/3224380‑3224659                                                                                                                                             |                                                                                                                                             tcggATCAGCCGCCGGGGCCGGGCTTGAAAGAGTACAAACAGGTTATCGCGCGGGTAAAAATCCACGCGCGGGAGCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCa                                                                                                                                            <  2:215899/140‑1 (MQ=255) tCCGAGCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGATTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCa                                                                                                                                            <  2:136702/143‑1 (MQ=255)    gATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGc                                                                                                                                         >  1:564330/1‑143 (MQ=255)        ggACGCCGGGACGGGGCGTGAAGGAGTACATACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCagag                                                                                                                                     <  1:75269/142‑1 (MQ=255)        aGACGCCGGGACCGGGCCTGAAAGAGTACAGACAGGTTATCGCGCCGGTAAACATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCagag                                                                                                                                     <  2:609543/143‑1 (MQ=255)        aGACGCCGGGACCGGGCCTGAAAGAGTACACACAGGTTATCACGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCatag                                                                                                                                     >  2:530265/1‑143 (MQ=255)                ggACCGGGCCGGAAAGGGTGGCACAAGGTTAGCGCGGCGGTAAAACTCCAGACGCGGGATCTGAGTCGCCTGGAGTTGAAAGTCGAAAATAGACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTgcgcgc                                                                                                                             <  1:656249/143‑1 (MQ=255)                     ggtccTGAAAGAGTACGAACAGGTGATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAg                                                                                                                        <  1:608860/140‑1 (MQ=255)                     gggCCTGAACGAGTACAAACAGTTTATCGAGCAATCCACAATCCACTCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAg                                                                                                                        <  1:295351/143‑1 (MQ=255)                        cccgAAAGAGTACTACCAGGTTATCGCGCCGCTAAGTATCCACGCGCGTGTGCTGACTCGCGGCGAGTGCAAACTCGAAAATCCACCTTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTg                                                                                                                     <  1:79998/140‑1 (MQ=255)                        ccTGAAAGAGTACAAGCAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTGGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTg                                                                                                                     <  1:147385/143‑1 (MQ=255)                         cTGAAAGAGTACAAACAGGTTAGCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGa                                                                                                                    <  2:364875/143‑1 (MQ=255)                           gAAAGAGTACAAACAGGTTATGGCGCCGGTAAAAAGCCACGCGCGGGATCGGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGaaa                                                                                                                  <  2:347111/143‑1 (MQ=255)                           gAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGaac                                                                                                                  >  2:621348/1‑142 (MQ=255)                             aaGAGTACAAACAGGTGATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACg                                                                                                                <  1:343776/143‑1 (MQ=255)                                      aaCAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCCGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTAGAAGACTACGCCCTGCACGCCGAGGCGCGCGCAGAAGGTGAAAGGCCGGCGACg                                                                                                       >  1:45068/1‑143 (MQ=255)                                           gTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGCGTTGAACGTCGAAACTAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCCACccagcg                                                                                                  >  1:605739/1‑142 (MQ=255)                                             tATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAACATAAACTGTGGGTTACCACGCTTGATGACTACACCCTGCAAGGAGAGGGGACCCCCCGAGGCGAAACGCTCGCGGCGCAGCAGa                                                                                                >  1:652501/1‑143 (MQ=255)                                              aTCGCGTCGGTAAAAATCCACGCGCGGGCTCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGAt                                                                                               <  1:274955/143‑1 (MQ=255)                                                  cgcCGGTAAAAATCCACGCGCGGGTTCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTaaa                                                                                           <  2:170488/143‑1 (MQ=255)                                                             acgcaggcgcgGCACGTGGCTCGAGGGGGGTTGAAGGGCGAAAATAAGCTGTGGTTTACCACGCTTGATGACTACCCCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGt                                                                                <  1:136206/137‑1 (MQ=255)                                                                  cgcgcgGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTgcgc                                                                           <  2:232549/143‑1 (MQ=255)                                                                   gcgcgGGATCTAACTCGCGGCGAGATGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATACCTACACCCTACACGCAGAGGTACGCGCCGAAGGAGAAACACTCGCGACGAAGCAGAACAAACAgcgccccctacacac                                                                          >  1:186433/1‑132 (MQ=255)                                                                         gctCTGACTCGCGGCGAGGTGAAAGTCGAGTATACACTCGGGCGTACCGCGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAg                                                                    <  1:137839/141‑1 (MQ=255)                                                                         gATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAg                                                                    <  2:564330/143‑1 (MQ=255)                                                                                 tCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGCGCAGCGAAGccc                                                            >  2:356451/1‑143 (MQ=255)                                                                                 tCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGccc                                                            >  2:552630/1‑143 (MQ=255)                                                                                    cggcgAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGCACAGCGAAccccccc                                                         >  1:28819/1‑142 (MQ=255)                                                                                           ttGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTACACTGCGCGACGTGGCGCCGAACAGCGAAGcccccacgccgac                                                  >  1:305306/1‑135 (MQ=255)                                                                                                  tCGAAACTAAACTGTGGTTTACTACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTg                                           <  1:571019/143‑1 (MQ=255)                                                                                                   cGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGc                                          <  2:374693/143‑1 (MQ=255)                                                                                                   cGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGACCGCCGAAGGTGAACCGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGc                                          >  2:155447/1‑143 (MQ=255)                                                                                                    gtaagtAAACTGTGCTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGcc                                         <  2:119479/138‑1 (MQ=255)                                                                                                              tgtgGGTGACCGCTCTTGGTGACTATACCCTGCGCGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGAc                              <  1:602125/143‑1 (MQ=255)                                                                                                                            cTTGATTACTACACCCTGCACGCATAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGtt                <  2:345342/143‑1 (MQ=255)                                                                                                                               gaggaCTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCt             <  1:149032/140‑1 (MQ=255)                                                                                                                                  gaCTACACCCTGCTCGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGGTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCaa          <  2:593409/143‑1 (MQ=255)                                                                                                                                  gaCTACACCCTGCACGCATAGGTGCGCGCCGAAGGCGCAACGCTCGCGACGCAGCAGATGAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCTCCTTGCAGATCTCGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCaa          <  1:46793/143‑1 (MQ=255)                                                                                                                                  gaCTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CCGCTGGACGCCCGCGACGCGTTCCTCaa          >  2:575095/1‑143 (MQ=255)                                                                                                                                   aCTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGAACACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGCTCCTCAAc         >  1:131105/1‑143 (MQ=255)                                                                                                                                    cTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACa        <  2:42899/143‑1 (MQ=255)                                                                                                                                      acacCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCCAACAGCGAAGCCCACTTATAAAAC‑CATTAACGTCAGCTGGACGCCCGCGAAGCGTTCCTCAACAtt      >  2:488098/1‑143 (MQ=255)                                                                                                                                       cacCCTGCACGCAGAGGTGCGCGCCGAAGGCAAGAATCCCGCTGCGCAGCAGATTAAACTGCGCGGCGATGCGCCGAACAGCGCACCCCCCTCGCAGATCACGCTGCCC‑CAGCCGCACGCCCGCCAAGCGATCCTCAACCTTa     >  1:171377/1‑143 (MQ=255)                                                                                                                                        acCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAACCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGCAGCGGTCCTCAACAttgc    >  2:294303/1‑141 (MQ=255)                                                                                                                                         ccccGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCGCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACg   <  2:453768/143‑1 (MQ=255)                                                                                                                                         cccTGCACGCAGAGGTGCGCGCCGAAGGTGAACCGCTCGCGACGCAGCAGATTAAACTGCGAGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACg   <  1:202055/143‑1 (MQ=255)                                                                                                                                         cccTCCCCGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACg   <  2:17241/143‑1 (MQ=255)                                                                                                                                          ccTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAGCCGTTCCTCAACATTACgg  >  2:675969/1‑143 (MQ=255)                                                                                                                                          ccTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACgg  <  1:494082/143‑1 (MQ=255)                                                                                                                                             |                                                                                                                                             TCCGATCAGACGCCGGGACCGGGCCTGAAAGAGTACAAACAGGTTATCGCGCCGGTAAAAATCCACGCGCGGGATCTGACTCGCGGCGAGTTGAAAGTCGAAAATAAACTGTGGTTTACCACGCTTGATGACTACACCCTGCACGCAGAGGTGCGCGCCGAAGGTGAAACGCTCGCGACGCAGCAGATTAAACTGCGCGACGTTGCGCCGAACAGCGAAGCCCCCTTGCAGATCACGCTGCCG‑CAGCTGGACGCCCGCGAAGCGTTCCTCAACATTACGG  >  NC_000913/3224380‑3224659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.