Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,132,787	A→C	I204S (ATC→AGC)	wcaA ←	putative glycosyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,132,787	0	A	C	100.0%	49.5 / NA	21	I204S (ATC→AGC)	wcaA	putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/7);  total (14/7)

GGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTG  >  NC_000913/2132659‑2132923                                                                                                                                 |                                                                                                                                         gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTCTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGACTCTGCGTCGCctcg                                                                                                                            >  1:306355/1‑142 (MQ=255) gggTAAACAGCCGATATTCTTTGCTGGCGCGGTCGAATTTGTCTTGGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCctct                                                                                                                            <  2:69685/143‑1 (MQ=255)     aaacgtcTGTTAGTTTTTTGTTGCGCGGTCGAATTTGTCTGTGTTCTTGCCGTAATAGTGGCAGTACCCGGAGAATTTTTGCGGTGACGCGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                        <  1:470509/137‑1 (MQ=255)          gCTGATATTTTTTGCTGGCGCGGCCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc                                                                                                                   <  2:11940/143‑1 (MQ=255)                   ttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGGACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCGTGTCACCGTGATTGACATGCAGGCTCTGCGTCGCCTCTTCTACTTTGCTCGGTGCg                                                                                                          >  1:94817/1‑143 (MQ=255)                         tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCAGCGGTTCGCCGTAc                                                                                                    >  2:158225/1‑143 (MQ=255)                              cgGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccac                                                                                               >  2:394305/1‑143 (MQ=255)                                gTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacaa                                                                                             >  2:325961/1‑141 (MQ=255)                                                              gTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTGCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                               <  2:218871/143‑1 (MQ=255)                                                                        ccGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGAGAGATGTCGTAATCCTGTGCCGCTTTCAg                                                     >  2:454621/1‑143 (MQ=255)                                                                                  ttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCTATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTCTCg                                           >  2:24861/1‑143 (MQ=255)                                                                                             gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTACTCCTGTGCCGCTTTCCGTTTGGTAGCGAAAAAACAatc                                >  2:246069/1‑143 (MQ=255)                                                                                             gAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACACACActc                                >  1:562187/1‑143 (MQ=255)                                                                                                gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTAATCCCCCACCATCCGCAGGAAGATGTCGTAATCCTGAGCCGCGTGCAGTTCGGAACAAAAAAAACAGTCttt                             >  1:433797/1‑143 (MQ=255)                                                                                                 tGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCACGACGATGTCGTAATCCTGTGCCGCTCTCAGCTCGGTCTCGCACCAACCCTCcttc                            >  1:71547/1‑142 (MQ=255)                                                                                                                   gattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTACCGAACAAACACACTTTGAAACGCCATGCCCAGGTa          >  1:452400/1‑143 (MQ=255)                                                                                                                    ctttatatGCACGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAATATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaa         <  1:267367/139‑1 (MQ=255)                                                                                                                     ttgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGACGACGTCGTAGTCCTGTGCCGCTCTCAGTTCGGTATCGAACAAACACCCTTTGACACGCCATGCCCAGGTaca        >  1:104365/1‑141 (MQ=255)                                                                                                                      tgatATGCAGGCTCTGCGTCCCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAAAAAACACACTTACAAGCGCCATGCCCAGGTAAAg       <  1:410553/143‑1 (MQ=255)                                                                                                                          atGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCGGTCCTCCACCACCATCCGCAGGCAGATGTCGCCATGATGTCTCGTGTTCGGTACGGTATGGGACAAACACTATTGGCAACACCACGCGCAGGtcagtacct   >  1:323581/1‑135 (MQ=255)                                                                                                                           tGCCGGCTCTGCGGCGCCTCGTGTACTTTCCGCGGGTCGCCGTACTCGGCCTCCATCCGGAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTg  <  1:475649/143‑1 (MQ=255)                                                                                                                                 |                                                                                                                                         GGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTG  >  NC_000913/2132659‑2132923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑