Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	702,066	G→C	P229R (CCT→CGT)	nagA ←	N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	702,066	0	G	C	100.0%	53.4 / NA	18	P229R (CCT→CGT)	nagA	N‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (11/7);  total (11/7)

TGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCA  >  NC_000913/701929‑702201                                                                                                                                          |                                                                                                                                        tGGCGGCAGTAACCAGACACAGTTGGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCa                                                                                                                                    <  1:85338/143‑1 (MQ=255)                  cagagttgctcgagtttCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACgg                                                                                                                   <  1:63264/129‑1 (MQ=255)                           tCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTACTCACCATGCAGGCCATCAGCAATAATACCGCAATAAATGTCCGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGCCCGGTAATATACGGCCTCGCGta                                                                                                          >  1:351554/1‑142 (MQ=255)                             gcgtgtCAGCCGTGTAGCGTTGCGAATGTTGGCGCAATCAACATGCAGGCCATCAGCAATAATACCGCAATCAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGt                                                                                                        <  2:189220/138‑1 (MQ=255)                                      aCGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTAGCGACCGGTAATATACGGCATCGCGGTGTGCAGCTggg                                                                                               >  2:27772/1‑143 (MQ=255)                                      aCGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTACACGACCGGTAATATACGGCATCGAGTTGTAGAGAgggg                                                                                               >  2:77435/1‑143 (MQ=255)                                       cGTTGATCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGCTCGCGCCCGCCAGGCTACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGt                                                                                              <  1:326656/143‑1 (MQ=255)                                                  gCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTaa                                                                                   <  1:502325/143‑1 (MQ=255)                                                   cGAATGTTGGCTTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAAt                                                                                  <  2:236556/143‑1 (MQ=255)                                                                     aCATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGGTGGGTGGCCAAGGTAATCCCCGCGCGGAAACCGGc                                                                >  2:70261/1‑143 (MQ=255)                                                                          cAGGCCATCAGCAATAATACCGCAATAAAGGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCAAGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAAGCCCCGCGCGGGAACCGGCTtgtg                                                           >  2:121677/1‑143 (MQ=255)                                                                             gCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGCAACCggagagtgatt                                                        >  1:484899/1‑134 (MQ=255)                                                                                  cAGCAACAATACCGCAATAAATGTCAGCTTCGTCGAGGTTCGCGCCCGCCAGGCCACGTTCACGACCGGTAAGATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTc                                                   <  2:443728/143‑1 (MQ=255)                                                                                              cGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTCCAGCTGGGTGGCAACGGCACTCCCCGCGCGGAAACCGGCTTCTGCTGCTTTCACCGTCGCGttc                                       >  1:297040/1‑142 (MQ=255)                                                                                                       tGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCg                              >  2:489279/1‑143 (MQ=255)                                                                                                         tCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGATGTACAGATGGGTGGTAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCCTCTTGCAACGGCGCGGTGGAGGGAcacgc                            >  2:506964/1‑139 (MQ=255)                                                                                                              ttCGTCGAGGATCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGaaa                       >  2:156070/1‑143 (MQ=255)                                                                                                                         tCGCGCCCGCCAGGCCACGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCCACCTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCg            >  1:375945/1‑143 (MQ=255)                                                                                                                                 gccgggccacGTTCACGACCGGTAATATTCGCCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTGGGTTTGGGTTTACCTACAAAAACCACAATCCCGGCATTTGc    <  1:288208/143‑1 (MQ=255)                                                                                                                                   ccggccacgTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCa  <  1:251872/141‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACCGCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGGCAAAGGTAATCCCCGCGCGGAAACCGGCTTTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCA  >  NC_000913/701929‑702201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑