New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1428264NA (NA)17 (0.420) 3/272 6.3 100% coding (463/981 nt) insH1 IS5 transposase and trans‑activator
?NC_000913 = 2066866 0 (0.000)coding (456/1017 nt) insH1 IS5 transposase and trans‑activator
Rejected: Coverage evenness skew score above cutoff.

CATGCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTcttggt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1428123‑1428264
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTTGGTCGATGCCACCATCATTGAGGCACCCAGCTCGACCAAGAACAAAGAGCAGCAACGCGATCCGGAGATGCATCAGACCAAGAAAGGCAATCAGTGGCACTTTGGCATGAAGGCCCACATTGGTGTCGATGCCAAGAGCGGCCTG  <  NC_000913/2066866‑2066719
                                                                                                                                                                                                                                                                                                  
CATGCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCTGATCGCGTTGCTGCTCTTTGTTC                                                                                                                                                     <  2:322811/143‑1
CATGCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTC                                                                                                                                                     <  1:566110/143‑1
CATGCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTC                                                                                                                                                     <  2:547799/143‑1
CATGCTCGTTGGCCGCGGGGGTGCCCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTC                                                                                                                                                     <  1:666912/143‑1
 gTGCGCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCT                                                                                                                                                    <  1:206126/142‑1
 cTGCTCGTTGGCCGCCGTGGTGACCAGGCTGTGTGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGGCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCT                                                                                                                                                    <  2:160053/142‑1
  tggTCGTTGGGCGCGGTGGCGACCAGGCTGTCGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTT                                                                                                                                                   <  1:354927/140‑1
  tcCTCGTGGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTT                                                                                                                                                   <  1:694016/141‑1
  TGCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTn                                                                                                                                                   <  2:199388/143‑2
  TGCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTT                                                                                                                                                   <  1:723805/143‑1
  TGCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTT                                                                                                                                                   <  1:310370/143‑1
  TGCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTT                                                                                                                                                   <  2:190328/143‑1
  TGCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTT                                                                                                                                                   <  2:4123/143‑1
  TGCTCGTTGGCCGCGGTGGTGACCAGGCTGGGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCCCCGGATCGCGTTGCTTCTCTTTGTTCTT                                                                                                                                                   <  1:91581/143‑1
  TGCTCGTTGGCCGCGGGGGTGACCAGGCTGTGGGTCAGGCCACTCTTTGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTT                                                                                                                                                   <  1:436226/143‑1
  TGCTCGGTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCGCTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTT                                                                                                                                                   <  2:252347/143‑1
   tCTCGTTGGCCGCGGTGGTGACGAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTG                                                                                                                                                  <  1:525763/142‑1
   gcgCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTG                                                                                                                                                  <  2:680435/140‑1
   GCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTTGCATCGACACCAATGTGGACCTTCATGCCAAAGTGCCACTGGTTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCCTTGCTGCTCTTTGTTCTTG                                                                                                                                                  >  2:231335/1‑143
   GCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTGATGCCAACGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTG                                                                                                                                                  <  1:10571/143‑1
   GCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTG                                                                                                                                                  <  1:417139/143‑1
   GCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTG                                                                                                                                                  <  2:712342/143‑1
   GCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTG                                                                                                                                                  >  1:83232/1‑143
    CTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCCAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGG                                                                                                                                                 >  2:423659/1‑143
    CTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGG                                                                                                                                                 >  2:507302/1‑143
    CTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGG                                                                                                                                                 <  2:426609/143‑1
    CTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCAACTCTTGGCATCGACACCAATGTGGGCCGTCATGCCAAAGTGCCACTGCTCGCCTTTCGTGGTCTGGTGCGGCTACGGGTGGCGTTGCTGCTCGCTGGTCTTGG                                                                                                                                                 >  1:370732/1‑143
    CTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGGCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGAATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGG                                                                                                                                                 >  2:486943/1‑143
     TCGTTGGCCTCTGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGGT                                                                                                                                                <  2:233223/143‑1
     TCGTTGGCCGCGGTGGTGCCCCGGCTGTGGGTCAGGCCCCTCTGGGCATCGACACCAATGGCGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGGT                                                                                                                                                <  1:255306/143‑1
     TCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCAAAGGCGCCACTGATTGCCTTTCTTCGTCCGATGCATCTCCGGATCGCGTTGATGCTCTTTGTTCTTGGT                                                                                                                                                >  1:181268/1‑143
      cgcTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAACGTGCCACTTATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGGTC                                                                                                                                               <  2:461610/140‑1
      CGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTTCTGCTCTTTGTTCTTGGTC                                                                                                                                               <  2:730726/143‑1
      CGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGGTC                                                                                                                                               >  2:547308/1‑143
      CGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGGTC                                                                                                                                               <  1:128697/143‑1
      CGTTGGCCGCGGTGGTGACCAGGCTGTGCGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGGTC                                                                                                                                               <  2:401941/143‑1
       GTTGGCCGGGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGGTCG                                                                                                                                              <  1:106135/143‑1
       GTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTGGTTCTTGGTCG                                                                                                                                              >  2:70503/1‑143
       GTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGCTCTGATGCATCTCCGGATCGCGTTGCTGCTATTTGATCTTGGTCG                                                                                                                                              >  2:408777/1‑143
        ttcGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCACCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGGTCGA                                                                                                                                             <  2:12829/140‑1
        TTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGGTCGA                                                                                                                                             >  2:152235/1‑143
          GGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGCTGCATCTCCGGATCGCGTTGCTGCTCCTTGTTCTTGGTCGAcc                                                                                                                                           >  2:659656/1‑141
              GCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTACTGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATACATCACCGGATCGCGTTGCTGCTCGGTGTTCTTGGTCacgccggg                                                                                                                                       >  1:456535/1‑135
                                           ACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCAGCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGGTCGATGCCACCATCATTGAGGCACCCAGCTCGACCAAGA                                                                                                          <  2:72491/143‑1
                                            CTCTTGGCATCGACACCAATGTGGGCCTTCATGGCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCACCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGGTCGATGCCACCATCATTGAGGCACCCAGCTCGACCAAGAA                                                                                                         <  1:353532/143‑1
                                            CTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGGTCGATGCCACCATCATTGAGGCACCCAGCTCGACCAAGAA                                                                                                         <  1:658759/143‑1
                                            CTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTCTTGGTCGATGCCACCATCATTGAGGCACCCAGCTCGACCAAGAA                                                                                                         <  1:656987/143‑1
                                                TGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCGTTGTTCTTGGTCGATGCCACCATCATTGAGGCACCCAGCTCGACCAAGAACAAA                                                                                                     <  2:546481/143‑1
                                                                                                                                   gctcagggcgccgTGGTCGAGGCCACCGTGATTGAGGCACCCAGCTCGGCCAAGAACAAAGAGCAGCAACGCGATCCGGAGATGCATCAGACCAAGAAAGGCAATCAGTGGCACTTTGGCATGAAGGCCCACATTGGTGTCGA                  <  1:330588/130‑1
                                                                                                                                              CTTGGTCGATGCCACCATCATTGAGGCACCCAGCTCGACCAAGAACAAAGAGCAGCAACGCGATCCGGAGATGCATCAGACCAAGAAAGGCAATCAGTGGCACTTTGGCATGAAGGCCCACATTGGTGGCGATGCCACGAGag       >  1:724071/1‑141
                                                                                                                                                   TCGATGCCACCATCATTGAGGCACCCAGCTCGACCAAGAACAAAGAGCAGCAACGCGATCCGGAGATGCATCAGACCAAGAAAGGCAATCAGTGGCACTTTGGCATGAAGGCCCACACTGGTGACGATGCAAAGAGCGGCCcg  >  1:401053/1‑141
                                                                                                                                                   TCGATGCCACCATCATTGAGGCACCCAGCTCGACAAAGAACAAAGAGCAGCAACGCGATCCGGAGATGCGTCAGACCAAGAAAGGCAACCAGTGGCACTTTGGCATGACGGCCCACATTCGCGCCGATGCCAAGAGCGGCCTG  >  2:105603/1‑143
                                                                                                                                                   TCGATGCCACCATCATTGAGGCACCCAGCGCGACCAAGAACAAAGAGCAGCAACGCGATCCGGAGATGCATCAGACCAAGAAAGGCAATCAGAGGCACTCTGGCACGAAGGCCCACACCGGCGCCGATGCCAAGAGCGGCCcg  >  1:496292/1‑141
                                                                                                                                                                                                                                                                                                  
CATGCTCGTTGGCCGCGGTGGTGACCAGGCTGTGGGTCAGGCCACTCTTGGCATCGACACCAATGTGGGCCTTCATGCCAAAGTGCCACTGATTGCCTTTCTTGGTCTGATGCATCTCCGGATCGCGTTGCTGCTCTTTGTTcttggt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/1428123‑1428264
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTTGGTCGATGCCACCATCATTGAGGCACCCAGCTCGACCAAGAACAAAGAGCAGCAACGCGATCCGGAGATGCATCAGACCAAGAAAGGCAATCAGTGGCACTTTGGCATGAAGGCCCACATTGGTGTCGATGCCAAGAGCGGCCTG  <  NC_000913/2066866‑2066719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.